Protein adsorption, desorption, and aggregation mediated by solid-liquid interfaces.

Abstract:

:Adsorption of proteins to solid-fluid interfaces is often empirically found to promote formation of soluble aggregates and larger, subvisible, and visible particles, but key stages in this process are often difficult to probe directly. Aggregation mediated by adsorption to water-silicon oxide (SiOx) interfaces, akin to hydrated glass surfaces, was characterized as a function of pH and ionic strength for alpha-chymotrypsinogen (aCgn) and for a monoclonal antibody (IgG1). A flow cell permitted neutron reflectivity for protein layers adsorbed to clean SiOx surfaces, as well as after successive "rinse" steps. Aggregates recovered in solution after gently "rinsing" the surface were characterized by neutron scattering, microscopy, and fluorescence spectroscopy. IgG1 molecules oriented primarily "flat" against the SiOx surface, with the primary protein layer desorbed to a minimal extent, whereas a diffuse overlayer was easily rinsed off. aCgn molecules were resistant to desorption when they appeared to be unfolded at the interface, but were otherwise easily removed. For cases where strong binding occurred, protein that did desorb was a mixture of monomer and small amounts of HMW aggregates (for aCgn) or subvisible particles (for IgG1). Changes in adsorption and/or unfolding with pH indicated that electrostatic interactions were important in all cases.

journal_name

J Pharm Sci

authors

Perevozchikova T,Nanda H,Nesta DP,Roberts CJ

doi

10.1002/jps.24429

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

1946-1959

issue

6

eissn

0022-3549

issn

1520-6017

pii

S0022-3549(15)30083-6

journal_volume

104

pub_type

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