Abstract：
:Surface adsorption of two monoclonal antibodies (mAb1 and mAb2), with widely different hydrophobicity and self-association behavior in solution, was examined by quartz crystal microbalance with dissipation monitoring to understand how adsorption and protein self-interactions near the surface are impacted by their intrinsic properties. The dependence of mass and viscoelastic properties of the adsorbed protein layer on the type of surface, presence of a surfactant, protein concentration, and pH were examined. Adsorption was significantly reduced in the presence of surfactant for both proteins, but for the more hydrophobic mAb2, residual adsorption remained on polystyrene (PS) and Teflon surfaces. Protein concentration had little impact on the adsorbed protein mass for silicon dioxide surface but had a significant impact for PS and Teflon surfaces. At high protein concentrations, an irreversible layer formed first upon which a reversible layer builds. Reversible adsorption was significantly greater at higher protein concentrations and significantly higher for mAb2, consistent with its higher propensity to reversibly self-associate in solution. The viscoelastic properties suggest that adsorbed protein layer at high protein concentrations is more hydrated. The adsorbed protein layer at lower pH was more hydrated, and possibly more unfolded, consistent with the behavior of the antibody in bulk solution.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Oom A,Poggi M,Wikström J,Sukumar Mdoi
10.1002/jps.22771subject
Has Abstractpub_date
2012-02-01 00:00:00pages
519-29issue
2eissn
0022-3549issn
1520-6017pii
S0022-3549(15)31700-7journal_volume
101pub_type
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