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Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus.

Abstract：

:During dengue outbreaks, acute diagnosis at the patient's point of need followed by appropriate supportive therapy reduces morbidity and mortality. To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of dengue virus (DENV). We used a quencher to reduce nonspecific amplification. The assay does not require expensive thermocyclers, utilizing a simple water bath to maintain the reaction at 63 °C. Results can be visualized using UV fluorescence, handheld readers, or lateral flow immunochromatographic tests. We report a sensitivity of 86.3% (95% confidence interval [CI], 72.7-94.8%) and specificity of 93.0% (95% CI, 83.0-98.1%) using a panel of clinical specimens characterized by DENV quantitative reverse transcription-polymerase chain reaction. This pan-serotype DENV RT-LAMP can be adapted to field-expedient formats where it can provide actionable diagnosis near the patient's point of need.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Dauner AL,Mitra I,Gilliland T Jr,Seales S,Pal S,Yang SC,Guevara C,Chen JH,Liu YC,Kochel TJ,Wu SJ

doi

10.1016/j.diagmicrobio.2015.05.004

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

30-6

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(15)00157-1

journal_volume

83

pub_type

杂志文章

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