Abstract:
:Bisphenol A (BPA) is shown to be the endocrine disruptor that induces reproductive dysfunction in male animals. In this study, we aim to probe the effects of BPA exposure on induction of autophagy in goat Sertoli Cells (gSCs), as well as the relationship between autophagy and apoptosis. Results indicated that exposure to BPA (100, 200, 300, 400, 500 and 600μM) decreased the cell viability in a concentration-dependent manner. Exposure of gSCs to 500μM BPA for 12h resulted in in vitro triggered loss of mitochondrial membrane potential (ΔΨm) and increased reactive oxygen species (ROS) production. Apoptosis with an increase in Bax:Bcl-2 ratio and higher rates of autophagy, such as autophagosome formation and increased expression of autophagy-related markers were also induced in gSCs exposed to 500μM BPA. Furthermore, treatment with 350nM Rapamycin (Rap, autophagy activator) alleviated a decrease in cell viability, intracellular ROS production, and reduction of ΔΨm, as well as decreasing apoptosis. Collectively, our results indicated that gSCs viability was disrupted after BPA treatment through affecting ROS production, mitochondrial membrane potential and inducing autophagy/apoptosis.
journal_name
Environ Toxicol Pharmacoljournal_title
Environmental toxicology and pharmacologyauthors
Zhang Y,Han L,Yang H,Pang J,Li P,Zhang G,Li F,Wang Fdoi
10.1016/j.etap.2017.07.014subject
Has Abstractpub_date
2017-10-01 00:00:00pages
137-147eissn
1382-6689issn
1872-7077pii
S1382-6689(17)30203-Xjournal_volume
55pub_type
杂志文章abstract::Exposure of HL-60 cells, a human myeloid cell line, to 500μM etomidate for 24h reduced cell viability and increased nitric oxide production and mitochondrial permeability transition pore (mPTP) opening. Preconditioning (1h) with 1μM etomidate 4h before exposure to the 500μM dose of etomidate attenuated those detriment...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2010.02.003
更新日期:2010-05-01 00:00:00
abstract::To address the growing need for scientifically valid and humane alternatives to developmental neurotoxicity testing (DNT), we propose that basic research scientists in developmental neurobiology be brought together with mechanistic toxicologists and policy analysts to develop the science and policy for DNT alternative...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2004.12.035
更新日期:2005-05-01 00:00:00
abstract::The Cd has been recognized as one of the most toxic environmental and industrial pollutants due to its ability to induce disturbances in several organs and tissues following either acute or chronic exposure. This review accounts for the recent evidence on its mechanisms to induce neurotoxicity, the role of the blood-b...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2006.11.009
更新日期:2007-05-01 00:00:00
abstract::Recently, electromagnetic fields around ultra-high voltage transmission lines have received considerable attentions for their potential biological effects. This study aimed to investigate the effects of static electric field (SEF) and power frequency electric field (PFEF) on cognition. Mice were exposed to SEF and PFE...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2019.01.001
更新日期:2019-02-01 00:00:00
abstract::17-beta-Estradiol is naturally converted in numerous organisms to various derivatives/metabolites, which may be excreted from the organism into its immediate external environment. There is a paucity of data regarding the biological effects of these derivatives/metabolites on aquatic organisms. Male channel catfish (20...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/s1382-6689(01)00062-x
更新日期:2001-03-01 00:00:00
abstract::The metabolites of oestrogenic substances of plant origin, phyto-oestrogens, have been proposed as cancer-protective agents in Asian and vegetarian populations. The two principle classes of these weak oestrogens are isoflavonoids and mammalian lignans. The former is derived from soya-based foods and the latter from oi...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/s1382-6689(99)00018-6
更新日期:1999-07-01 00:00:00
abstract::We previously reported that Astragalus polysaccharide (APS) extracted from Chinese medicine Astragalus membranaceus (Fisch.) Bge, attenuates hypertrophy of neonatal rat ventricular myocytes (NRVMs) induced by isoproterenol (Iso). The present study was designed to investigate the effects and the possible mechanism of A...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2015.03.014
更新日期:2015-05-01 00:00:00
abstract::Indole-3-carbinol (I3C) is extracted from cruciferous vegetables and is well known for its anti-cancer, antioxidant and anti-inflammatory effects. This study investigated the protective effect of I3C in hyperlipidemia zebrafish larvae and early life stage toxicity of I3C on zebrafish embryos/larvae. Zebrafish larvae w...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2019.103205
更新日期:2019-08-01 00:00:00
abstract::The protective effects of lobenzarit disodium against the toxicity of allyl alcohol were investigated in vitro using isolated rat hepatocytes and in vivo using mice. In mice, at i.p doses of 25, 50 and 100 mg/kg lobenzarit significantly decreased the activity of alanine amino transferase (ALT) in serum and the concent...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/s1382-6689(97)00149-x
更新日期:1997-06-06 00:00:00
abstract::The present study was undertaken to scientifically evaluate, validate and compare the cardioprotective effects of lisinopril (Lsp), an angiotensin converting enzyme (ACE) inhibitor and vitamin E (Vit E), an antioxidant in the setting of ischemia and reperfusion (I-R) injury. An open chest left anterior descending coro...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2012.12.003
更新日期:2013-03-01 00:00:00
abstract::The free radicals within the body, produced by metabolic activities or derived from environmental sources are relatively related to hepatoxicity. Since heavy metals including iron have the ability to produce free radicals, the liver glutathione system neutralizes them to protect cells against any damage. The objective...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2013.12.007
更新日期:2014-01-01 00:00:00
abstract::Paraquat (PQ) is a relatively safe and effective herbicide used all over the world. PQ is very toxic to all living organisms; and many cases of acute poisoning and death have been reported over the past decade. The main suggested potential mechanism for PQ toxicity is the production of superoxide radicals from the met...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2008.02.009
更新日期:2008-07-01 00:00:00
abstract::Acetylcholinesterase (AChE) is an important part of cholinergic nerves where it participates in termination of neurotransmission. AChE can be inhibited by e.g. some Alzheimer disease drugs, nerve agents, and secondary metabolites. In this work, metal salts aluminum chloride, calcium chloride, cupric chloride, ferric c...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2014.01.001
更新日期:2014-01-01 00:00:00
abstract::This study aimed to observe the therapeutic effects of magnesium lithospermate B on acute and chronic colitis induced by dextran sodiumsulfate (DSS) and the role of inflammasome complex (NOD-like receptor protein, NLRP; apoptosis-associated speck-like protein containing, ASC; caspase-1). Establishment of acute and chr...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2015.10.009
更新日期:2016-01-01 00:00:00
abstract::Effects of lethal and sublethal concentrations 15, 20, 26 and 35 μg/l of zeta cypermethrin on the gills of common guppies (L. reticulatus) were examined by light microscopy during 96 h exposure. The most common changes at all doses of zeta cypermethrin are the lifting of epithelial layer from gill lamellae and some ne...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/S1382-6689(03)00046-2
更新日期:2003-09-01 00:00:00
abstract::Long non-coding RNAs (LncRNAs) are a category of non-coding RNAs (ncRNAs) with a length of 200nt-100kb lacking a significant open reading frame. The study of lncRNAs is a newly established field, due in part to their capability to act as the novel biomarkers in disease. A growing body of research shows that lncRNAs ma...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.etap.2017.08.014
更新日期:2017-10-01 00:00:00
abstract::To evaluate the genotoxic effects of formaldehyde, acetaldehyde, acrolein and benzene on A549 cells, the in vitro γH2AX assay was used in combination with high content screening (HCS) technology. All aldehydes showed a significant genotoxicity in a dose/time-dependent effect on the induction of γH2AX. Benzene failed t...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2017.07.005
更新日期:2017-10-01 00:00:00
abstract::Exposure to lead (Pb) or polychlorinated biphenyls (PCBs) during early development has been associated with deficits in cognitive function in children (Pediatrics 87 (1991) 219; N. Engl. J. Med. 335 (1996) 783). These effects persist in the child long after exposure has ceased and body burdens have diminished. Despite...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/s1382-6689(02)00029-7
更新日期:2002-09-01 00:00:00
abstract::This study was aimed at documenting the kinetic time courses of selenium (Se) in accessible biological matrices of volunteers following controlled applications of therapeutic shampoo containing Se, to better elucidate the mechanisms by which shampoo-Se accumulates in hair and hence estimate the contribution of this so...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2010.02.001
更新日期:2010-05-01 00:00:00
abstract::Taurine (TAU) has protective effects on experimental liver fibrosis. The present study investigates whether benefits of TAU are mediated through attenuation of oxidative and nitrosative stresses. Liver fibrosis was induced in male Wistar rats by simultaneous administration of iron (0.5%, w/w) and ethanol (6g/kg/day) f...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2009.11.005
更新日期:2010-03-01 00:00:00
abstract::A thiamine deficiency disease termed M74 syndrome affects the yolk-sac fry of wild and feral Baltic salmon. However, very little is known about the pharmacokinetics of thiamine in salmon. This study examined the pharmacokinetics of thiamine hydrochloride injected intraperitoneally into feral and farmed Baltic salmon f...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2004.06.001
更新日期:2005-01-01 00:00:00
abstract::The present study examined the potential toxicity of 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and 1-butyl-3- methylimidazolium tetrafluoroborate ([Bmim]BF4) in the liver cells of zebrafish (Danio rerio) with different doses (20-160mg/L) on 7 and 14days. The effects of [Bmim]Cl and [Bmim]BF4 on acute toxicity, r...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2017.02.018
更新日期:2017-04-01 00:00:00
abstract::The aliphatic ether 1,6-dimethoxyhexane (DMH) was previously identified as a testicular toxicant. Testis protein extracts from control and DMH-treated rats were subjected to two-dimensional gel electrophoresis for comparison of protein expression profiles. MALDI-ToF peptide mass fingerprinting of differentially expres...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2007.04.001
更新日期:2007-09-01 00:00:00
abstract::This study was to investigate the combined toxicity of silica nanoparticles (SiNPs) and methylmercury (MeHg) on cardiovascular system in zebrafish (Danio rerio) embryos. Ultraviolet absorption analysis showed that the co-exposure system had high absorption and stability. The dosages used in this study were based on th...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2016.05.004
更新日期:2016-06-01 00:00:00
abstract::A water extract of the lyophilised fresh-water alga Anabaena flos-aquae enhanced substantially the release of [(3)H]acetylcholine ([(3)H]acetylcholine and [(3)H]choline) from cholinergic nerves of rat bronchi. Parallel experiments performed with the related species Anabaena lemmermannii did not demonstrate this effect...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/s1382-6689(97)10007-2
更新日期:1998-01-01 00:00:00
abstract::Diethylnitrosamine (DEN) treatment increases the generation of reactive oxygen species (ROS), apoptosis, necrosis and proliferation in the liver. Blueberries (BB; Vaccinium corymbosum L.) contain polyphenols and other active components and have high antioxidant capacities. We investigated the effect of BB pretreatment...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2013.05.014
更新日期:2013-09-01 00:00:00
abstract::Earthworms are the key soil organisms, contribute to many positive ecological services that could be degraded by pesticides and other soil pollutants such as heavy metals. Chemicals usually occur as mixtures in the environmental systems which can lead synergistic effects. The assessment and characterization of soil po...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.etap.2017.08.001
更新日期:2017-10-01 00:00:00
abstract::The aim of this work was to evaluate the Ascidian Embryo Teratogenicity assay (AET) as new alternative invertebrate model to test the developmental effects of the co-exposure to ethanol and fluconazole. Ciona intestinalis embryos were exposed to the azolic fungicide fluconazole, (FLUCO, 7.8-250μM), to ethanol (Eth, 0....
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2017.11.014
更新日期:2018-01-01 00:00:00
abstract::In order to better inform scientific decision making in the occupational environment, we need a better understanding of the toxicology of mixed exposures. In particular, we need an understanding of the dose-response relationship from the level of individual or population exposure down to the molecular level (and then ...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2004.01.014
更新日期:2004-12-01 00:00:00
abstract::Cadmium (Cd) is a major pollutant of environment. It can be fatal to human. In spite of bulk of research and literatures, the mechanism of a fatality against human is still not understood completely. Toxic and carcinogenic effects of Cd in rodents and humans are well known. However, effects of Cd on induction of apopt...
journal_title:Environmental toxicology and pharmacology
pub_type: 杂志文章
doi:10.1016/j.etap.2013.09.006
更新日期:2013-11-01 00:00:00