Human milk oligosaccharides in premature infants: absorption, excretion, and influence on the intestinal microbiota.


BACKGROUND:Human milk oligosaccharides (HMOs) shape the intestinal microbiota in term infants. In premature infants, alterations in the intestinal microbiota (dysbiosis) are associated with risk of necrotizing enterocolitis (NEC) and sepsis, and the influence of HMOs on the microbiota is unclear. METHODS:Milk, urine, and stool specimens from 14 mother-premature infant dyads were investigated by mass spectrometry for HMO composition. The stools were analyzed by next-generation sequencing to complement a previous analysis. RESULTS:Percentages of fucosylated and sialylated HMOs were highly variable between individuals but similar in urine, feces, and milk within dyads. Differences in urine and fecal HMO composition suggest variability in absorption. Secretor status of the mother correlated with the urine and fecal content of specific HMO structures. Trends toward higher levels of Proteobacteria and lower levels of Firmicutes were noted in premature infants of nonsecretor mothers. Specific HMO structures in the milk, urine, and feces were associated with alterations in fecal Proteobacteria and Firmicutes. CONCLUSION:HMOs may influence the intestinal microbiota in premature infants. Specific HMOs, for example those associated with secretor mothers, may have a protective effect by decreasing pathogens associated with sepsis and NEC, while other HMOs may increase dysbiosis in this population.


Pediatr Res


Pediatric research


Underwood MA,Gaerlan S,De Leoz ML,Dimapasoc L,Kalanetra KM,Lemay DG,German JB,Mills DA,Lebrilla CB




Has Abstract


2015-12-01 00:00:00














  • Selective impairment of taurine transport by cyclosporin A in a human placental cell line.

    abstract::We investigated, using a human placental choriocarcinoma cell line as a model, the effects of the immunosuppressive drug cyclosporin A on several placental transport systems mediating the transfer of glucose and amino acids from mother to fetus. The results of the investigation show that the transport system responsib...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Ramamoorthy S,Leibach FH,Mahesh VB,Ganapathy V

    更新日期:1992-07-01 00:00:00

  • Endogenous opioids do not mediate HCl-induced myocardial dysfunction.

    abstract::We evaluated the hypothesis that increased endogenous opioid activity mediates part or all of the left ventricular contractile and pump dysfunction previously demonstrated in HCl-induced metabolic acidemia. Eighteen Western newborn lambs were catheterized and instrumented; pacing wires were sutured to the right atrial...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Fisher DJ,Dreyer WJ,Tate C,Michael L,Smith EO,Entman M

    更新日期:1988-06-01 00:00:00

  • aEEG and neurologic exam findings correlate with hypoxic-ischemic brain damage severity in a piglet survival model.

    abstract:BACKGROUND:Newborn pigs offer theoretical advantages for studying newborn hypoxic-ischemic (HI) brain damage because of a development and structure similar to the human brain. However, the correlation between functional features and actual HI brain damage has not been reported. METHODS:Newborn pigs were examined daily...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Barata L,Cabañas A,Lafuente H,Vargas C,Ceprián M,Campa L,Jiménez-Sánchez L,Pazos MR,Alvarez FJ,Martínez-Orgado J

    更新日期:2019-03-01 00:00:00

  • Alpha-lactalbumin in human milk alters the proteolytic degradation of soluble CD14 by forming a complex.

    abstract::Mother's milk represents a foundational step in the proper development of newborn immunity. This is achieved, in part, through the action of numerous regulatory proteins such as soluble cluster of differentiation 14 (sCD14) found in significant quantities in human milk (~25-50 μg/mL). In adults, CD14 stimulates cytoki...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Spencer WJ,Binette A,Ward TL,Davis LD,Blais DR,Harrold J,Mack DR,Altosaar I

    更新日期:2010-12-01 00:00:00

  • Inhibitory effect of 5β-pregnane-3α,20β-diol on transcriptional activity and enzyme activity of human bilirubin UDP-glucuronosyltransferase.

    abstract::Prolonged unconjugated hyperbilirubinemia in infants associated with breast milk feeding is a common pediatric problem known as breast milk jaundice (BMJ). A polymorphic mutation (G71R) of bilirubin UDP-glucuronosyltransferase (UGT1A1) is a known cause of BMJ on the infantile side, but the responsible components of br...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Ota Y,Maruo Y,Matsui K,Mimura Y,Sato H,Takeuchi Y

    更新日期:2011-11-01 00:00:00

  • Changes in 5alpha-pregnane steroids and neurosteroidogenic enzyme expression in fetal sheep with umbilicoplacental embolization.

    abstract::Pregnane steroids have sedative and neuroprotective effects on the brain, due to interactions with the steroid-binding site of the GABAA receptor. In the adult brain, synthesis of the pregnane steroids is increased in response to stress. Therefore, we have used umbilicoplacental embolization to mimic chronic placental...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Nguyen PN,Billiards SS,Walker DW,Hirst JJ

    更新日期:2003-12-01 00:00:00

  • Cerebral metabolic response to neonatal hypoglycemia in growth-retarded dogs.

    abstract::The cerebral metabolic effects of hypoglycemia due to intrauterine growth retardation were studied in newborn dogs. Intrauterine growth retardation was induced in newborn dogs after 3 days of maternal nutritional deprivation (birth weight 251 +/- 7 versus 227 +/- 7 g, p less than 0.01). After birth, growth retarded pu...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Kliegman RM

    更新日期:1988-11-01 00:00:00

  • Effect of human milk or formula on gastric function and fat digestion in the premature infant.

    abstract::The effect of diet, human milk or formula, on gastric function (lipase and pepsin activity, pH, and volume) and intragastric digestion of fat was assessed in 28 appropriate for gestational age preterm infants (gestational age, 28.9 +/- 1.4, 29.1 +/- 0.9, 29.5 +/- 0.6 wk; birth weight, 1.00 +/- 0.14 to 1.18 +/- 0.07 kg...

    journal_title:Pediatric research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Armand M,Hamosh M,Mehta NR,Angelus PA,Philpott JR,Henderson TR,Dwyer NK,Lairon D,Hamosh P

    更新日期:1996-09-01 00:00:00

  • Effects of maternal diabetes or in vitro hyperglycemia on uptake of palmitic and arachidonic acid by rat embryos.

    abstract::Altered transfer of nutrients from mother to conceptus may be involved in the pathogenesis of the developmental disturbances in offspring of diabetic mothers. In our study, the embryonic uptake of a saturated (palmitic acid) and a nonsaturated (arachidonic acid) fatty acid was evaluated in a normal and a diabetes-like...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Engström E,Haglund A,Eriksson UJ

    更新日期:1991-08-01 00:00:00

  • Effect of ventilation on mechanical properties and pressure-flow relationships of immature airways.

    abstract::Ventilation of immature airways has been shown to result in pressure-induced deformation and alteration of mechanical properties. These changes in mechanical properties may alter the effect of compressive pressures on pressure-flow relationships. To test this hypothesis, unventilated (Group I, n = 8) and ventilated (g...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Penn RB,Wolfson MR,Shaffer TH

    更新日期:1988-05-01 00:00:00

  • Residual CD95-pathway function in children with autoimmune lymphoproliferative syndrome is independent from clinical state and genotype of CD95 mutation.

    abstract::Human autoimmune lymphoproliferative syndrome (ALPS) is caused by defective CD95-mediated apoptosis of lymphocytes. In most patients, heterozygous mutations within the CD95 gene are found. Mutated proteins interfere with CD95-signaling in a dominant-negative way. However, the penetrance of clinical disease is variable...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Fuchs H,Posovszky C,Lahr G,van der Werff ten Bosch J,Boehler T,Debatin KM

    更新日期:2009-02-01 00:00:00

  • Cerebrovasodilatory contribution of endogenous carbon monoxide during seizures in newborn pigs.

    abstract::Carbon monoxide (CO) and the excitatory amino acid glutamate both dilate cerebral arterioles in newborn pigs. The key enzyme in CO synthesis is heme oxygenase, which is highly expressed in neurons with glutamatergic receptor activity as well as cerebral microvessels. During seizures the extracellular level of glutamat...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Pourcyrous M,Bada HS,Parfenova H,Daley ML,Korones SB,Leffler CW

    更新日期:2002-05-01 00:00:00

  • Thyroid hemiagenesis is a rare variant of thyroid dysgenesis with a familial component but without Pax8 mutations in a cohort of 22 cases.

    abstract::Thyroid hemiagenesis is a rare form of thyroid dysgenesis of which some familial cases have been reported, including one associated with a heterozygous mutation in the Pax8 gene. However, the physiopathology remains not well known. The objectives of this study were 1) to describe the clinical features, 2) to look for ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Castanet M,Leenhardt L,Léger J,Simon-Carré A,Lyonnet S,Pelet A,Czernichow P,Polak M

    更新日期:2005-06-01 00:00:00

  • Myocardial dysfunction in group B streptococcal shock.

    abstract::A rabbit model of group B Streptococcal (GBS) shock was used to determine if myocardial dysfunction contributes to GBS shock and, if so, to ascertain if prostaglandins modulate this dysfunction. The infusion of heat-killed GBS (group I) produced a dramatic decrease in the first derivative of left ventricular pressure ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Peevy KJ,Chartrand SA,Wiseman HJ,Boerth RC,Olson RD

    更新日期:1985-06-01 00:00:00

  • Surface and cytoplasmic immunoglobulin expression in circulating B-lymphocytes in acute Kawasaki disease.

    abstract::Kawasaki disease (KD) is an acute vasculitis of young childhood predominantly affecting the coronary arteries. IgA plasma cells have been found to infiltrate vascular and nonvascular tissues in fatal acute KD. To determine whether IgA B-lymphocytes were increased in the peripheral blood of patients with KD, we perform...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Shingadia D,O'Gorman M,Rowley AH,Shulman ST

    更新日期:2001-10-01 00:00:00

  • Human milk cell migration and production of monocyte chemotactic factor: lack of activity.

    abstract::The lymphocytes of human breast milk have been previously shown to be immunologically competent while the monocytes have been described as actively motile in some reports but not in another. Cells were separated from milk samples collected by lactating women 2 to 9 days postpartum. Milk cell migration and production o...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Hawes CS,Jones WR

    更新日期:1985-10-01 00:00:00

  • Prostaglandin E1 opens the ductus venosus in the newborn lamb.

    abstract::There is in vitro evidence that the ductus venosus of the fetal and newborn lamb relaxes to administration of E-series prostaglandins. We performed this study to determine whether the ductus venosus of the newborn lamb could be reopened by an infusion of prostaglandin E1. Fifteen lambs between 4 and 28 h of life and s...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Morin FC 3rd

    更新日期:1987-03-01 00:00:00

  • Biochemical manifestations of oxygen toxicity in the newborn lamb.

    abstract::The purpose of this project was to study the role of lipid peroxidation in oxygen-induced lung injury in the newborn lamb. It was our hypothesis that injury to the microvascular bed of the lung by oxygen would coincide with a burst of peroxidative activity and would be accompanied by an increased rate of excretion of ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Hansen TN,Smith CV,Gest AL,Smith HW,Giesler M

    更新日期:1990-12-01 00:00:00

  • Hypoxia induces alteration of bone morphogenetic protein receptor signaling in pulmonary artery endothelial cell.

    abstract::Reduced expression of bone morphogenetic protein receptors (BMPR) has been implicated in the pathogenesis of pulmonary hypertension (PH), but changes in the intracellular signaling pathway of BMPR have not been fully understood. We hypothesized that BMPR signaling in pulmonary endothelial cells is altered during the d...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Takahashi K,Kogaki S,Matsushita T,Nasuno S,Kurotobi S,Ozono K

    更新日期:2007-04-01 00:00:00

  • Modest hypothermia provides partial neuroprotection for ischemic neonatal brain.

    abstract::Hypothermia is a frequent occurrence in newborns, and thermoregulatory management is a fundamental part of medical stabilization. Although modest reduction in brain temperature (2-3 degrees C) before ischemia provides neuroprotection in adults, the effect of modest hypothermia on immature brain has not been examined. ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Laptook AR,Corbett RJ,Sterett R,Burns DK,Tollefsbol G,Garcia D

    更新日期:1994-04-01 00:00:00

  • Perspectives from the Society for Pediatric Research: Decreased Effectiveness of the Live Attenuated Influenza Vaccine.

    abstract::The intranasal live attenuated influenza vaccine (LAIV), FluMist, has been widely appreciated by pediatricians, parents, and children alike for its ease of administration. However, concerns regarding lack of effectiveness in recent influenza seasons led to the CDC Advisory Committee on Immunization Practices (ACIP) re...

    journal_title:Pediatric research

    pub_type: 杂志文章,评审


    authors: Gill MA,Schlaudecker EP

    更新日期:2018-01-01 00:00:00

  • Mesenteric blood flow velocity and its relation to transitional circulatory adaptation in appropriate for gestational age preterm infants.

    abstract::We investigated the early postnatal changes of the mesenteric circulation and its relation to the systemic circulation in 15 preterm infants. The infants were studied before the first feeding on d 1 and pre- and postprandially on d 3, 4, 5, and 7. Blood flow velocity was measured by ultrasound Doppler in the superior ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Martinussen M,Brubakk AM,Vik T,Yao AC

    更新日期:1996-02-01 00:00:00

  • Purine dysfunction in cells from patients with adenosine deaminase deficiency.

    abstract::Conversion of adenosine to inosine is decreased in adenosine deaminase (ADA)-deficient fibroblasts at all concentrations of adenosine tested. Adenosine is not differentially toxic to ADA-deficient fibroblasts except at very high (5 X 10(-4) -1 X 10(-3) M) adenosine levels. Conversion of [14C] adenosine to GTP is not d...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Benke PJ,Dittmar D

    更新日期:1976-07-01 00:00:00

  • Fibroblasts expressing PDGF-receptor-alpha diminish during alveolar septal thinning in mice.

    abstract::In mice, secondary alveolar septal formation primarily occurs during a brief postnatal period and is accompanied by transient expansion of the interstitial lung fibroblast (LF) population. PDGF-A, which solely signals through PDGF-receptor-alpha (PDGF-Rα), is required for expansion, but the receptor's relevant downstr...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: McGowan SE,McCoy DM

    更新日期:2011-07-01 00:00:00

  • Receptor-mediated cAMP production in adult and infant ferret tracheal epithelium.

    abstract::Tracheal epithelial cells obtained from adult and infant ferrets were grown in primary culture in vitro. Cells from adult animals grew readily, and many ciliated cells were observed in the cultures. Successful cultures were derived from infant animals, but cell number in infant and adult cultures began to decrease aft...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Kercsmar CM,Chung Y,Davis PB

    更新日期:1991-07-01 00:00:00

  • Regional metabolic assessment of human brain during development by proton magnetic resonance spectroscopy in vivo and by high-performance liquid chromatography/gas chromatography in autopsy tissue.

    abstract::To study the course of regional metabolite concentrations during early brain development, we measured in vivo metabolites [N-acetyl-aspartate (NAA), choline-containing compounds, and myoinositol (M-Ino)] in the precentral area of the cerebrum by short echo-time single volume proton magnetic resonance spectroscopy and ...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Hüppi PS,Fusch C,Boesch C,Burri R,Bossi E,Amato M,Herschkowitz N

    更新日期:1995-02-01 00:00:00

  • Body composition during the first 2 years of life: an updated reference.

    abstract::Normative body composition during the first 2 y of life was derived from a prospective study of 76 children. We present 1) fat free mass (FFM) and its components, and fat mass (FM), 2) incremental growth rates partitioned into chemical components, and 3) age-specific and gender-specific constants for converting chemic...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Butte NF,Hopkinson JM,Wong WW,Smith EO,Ellis KJ

    更新日期:2000-05-01 00:00:00

  • Effect of insulin with concurrent amino acid infusion on protein metabolism in rapidly growing pubertal children with type 1 diabetes.

    abstract::Insulin treatment of prepubertal children with insulin-dependent diabetes improves body protein balance by decreasing the rate of protein degradation without stimulating protein synthesis. However, insulin also causes hypoaminoacidemia, so the inability of insulin to stimulate protein synthesis may have been limited b...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Godil MA,Wilson TA,Garlick PJ,McNurlan MA

    更新日期:2005-08-01 00:00:00

  • Renal hemodynamic responses to hypoxemia during development: relationships to circulating vasoactive substances.

    abstract::Chronically catheterized fetal lambs (n = 11, gestational age 111-139 days) and neonatal lambs (n = 20, postnatal age 4-30 days) were studied to explore during development the relationship of renal hemodynamic responses during hypoxemia to plasma epinephrine concentration (E), plasma norepinephrine concentration (NE),...

    journal_title:Pediatric research

    pub_type: 杂志文章


    authors: Weismann DN,Robillard JE

    更新日期:1988-02-01 00:00:00

  • Preventing injuries must be a priority to prevent disease in the twenty-first century.

    abstract::Injuries continue to be the leading cause of morbidity and mortality for children, adolescents, and young adults aged 1-24 years in industrialized countries in the twenty-first century. In this age group, injuries cause more fatalities than all other causes combined in the United States (U.S.). Importantly, many of th...

    journal_title:Pediatric research

    pub_type: 杂志文章,评审


    authors: Dorney K,Dodington JM,Rees CA,Farrell CA,Hanson HR,Lyons TW,Lee LK,and the Injury Free Coalition for Kids®.

    更新日期:2020-01-01 00:00:00