Abstract:
MAIN CONCLUSION:We introduced a novel combination of chromatographic techniques for the purification and analysis of a new UV-sunscreen mycosporine-like amino acid (MAA) in the terrestrial green alga Prasiola calophylla. Prasiola calophylla (Carmichael ex Greville) Kützing (Trebouxiophyceae, Chlorophyta) is a typical member of terrestrial algal communities in temperate Europe, where it regularly experiences various stress conditions including strong diurnal and seasonal fluctuations in ultraviolet radiation (UVR). As a photoprotective mechanism Prasiola species and other related Trebouxiophycean taxa synthesize a mycosporine-like amino acid (MAA) as natural sunscreen whose chemical structure was unknown so far. In the present study a new methodological approach is described for the isolation, purification and structural elucidation of this novel sunscreen in P. calophylla. The new compound exhibits an absorption maximum at 324 nm (in the short ultraviolet-A), a molecular weight of 333 and a molecular extinction coefficient of 12.393 M(-1) cm(-1), and could be identified as N-[5,6 hydroxy-5(hydroxymethyl)-2-methoxy-3-oxo-1-cycohexen-1-yl] glutamic acid using one- and two-dimensional (1)H and (13)C-NMR spectroscopy. As trivial name for this novel MAA we suggest 'prasiolin'. The ecologically essential function of prasiolin for UVR-protection in terrestrial algae of the Trebouxiophyceae is discussed.
journal_name
Plantajournal_title
Plantaauthors
Hartmann A,Holzinger A,Ganzera M,Karsten Udoi
10.1007/s00425-015-2396-zsubject
Has Abstractpub_date
2016-01-01 00:00:00pages
161-9issue
1eissn
0032-0935issn
1432-2048pii
10.1007/s00425-015-2396-zjournal_volume
243pub_type
杂志文章相关文献
PLANTA文献大全abstract::In both the seminal axis and lateral roots of Cucurbita pepo L. the formation of large central xylem elements and the commencement of secondary cambial activity occur 10-20 cm from the root tip. Concomitant with or slightly preceding these developments there are changes in the structure of the walls of endodermal cell...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00390280
更新日期:1973-03-01 00:00:00
abstract::Biphenyls and dibenzofurans are the phytoalexins of the Maloideae, a subfamily of the economically important Rosaceae. The carbon skeleton of the two classes of antimicrobial secondary metabolites is formed by biphenyl synthase (BIS). A cDNA encoding this key enzyme was cloned from yeast-extract-treated cell cultures ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-006-0435-5
更新日期:2007-05-01 00:00:00
abstract::Tobacco leaves (Nicotiana tabacum var. Wisconsin 38) submitted to anaerobic conditions behave in a manner similar to that of maize, sugarcane, or sorghum leaves (C4-plants); more precisely, a lag time in O2 release is exhibited when the leaves are exposed to light after treatment in the dark under pure nitrogen. Altho...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387973
更新日期:1977-01-01 00:00:00
abstract::The effect of low concentrations of 3,5-diiodo-4-hydroxybenzoic acid (DIHB) in promoting the elongation of light-exposed cress (Lepidium sativum L.) roots has been further examined. Aeration of the DIHB solution in which the roots were grown largely removed the growth promotion. The addition of ethylene or the ethylen...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00388968
更新日期:1975-01-01 00:00:00
abstract::Measurements of net CO2 exchange, malate accumulation, properties and capacity of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) in leaves of different ages of two short-day dependent Crassulacean acid metabolism (CAM) plants (Kalanchoe blossfeldiana v. Poelln. Tom thumb and K. velutina Welw.) show that, in both ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00393911
更新日期:1982-05-01 00:00:00
abstract::In the genome of Nostoc punctiforme PCC 73102, three functional β-carotene ketolase genes exist, one of the crtO and two of the crtW type. They were all expressed and their corresponding enzymes were functional inserting 4-keto groups into β-carotene as shown by functional pathway complementation in Escherichia coli. ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-013-1846-8
更新日期:2013-05-01 00:00:00
abstract::Experiments have been carried out to determine the basis for the dependence of chloroplast pigment synthesis on protein synthesis in dark-grown cells of Euglena gracilis greening in the light. The complete inhibition of chlorophyll synthesis brought about by actidione (10 μg/ml) when added half way through the greenin...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00406651
更新日期:1967-06-01 00:00:00
abstract::Localisation of α-amylase (EC 3.2.1.1) in barley aleurone cells treated with gibberellic acid has been achieved using protein A-gold-labelled polyclonal antibodies. Gold particles were located almost exclusively over the lumen of the rough endoplasmic reticulum and cisternae of the Golgi apparatus. The label was most ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392263
更新日期:1986-09-01 00:00:00
abstract::De-etiolation involves a number of phenotypic changes as the plants shift from a dark-grown (etiolated) to a light-grown (de-etiolated) morphology. Whilst these light-induced, morphological changes are thought to be mediated by plant hormones, the precise mechanism/s are not yet fully understood. Here we provide furth...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-007-0685-x
更新日期:2008-04-01 00:00:00
abstract::Cutin synthesis of Clivia miniata Reg. was studied by using intact leaves. Tritium-labelled hexadecanoic acid was used as precursor and was administered as droplets of micellar solutions to the upper surface of expanding leaves. Radiolabel was incorporated rapidly. Within 2 h, up to 10% of the label administered had b...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00395405
更新日期:1983-06-01 00:00:00
abstract::Changes in percentage organic carbon content were assessed during the first five weeks of growth of Uniculm barley (Hordeum vulgare) and Brussels sprouts (Brassica oleracea) plants grown in controlled-environment conditions at two constant temperatures, 16° and 22°C. Foliage (leaf laminae), stem, and root material was...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00392856
更新日期:1984-02-01 00:00:00
abstract::Canavanine is an arginine analogue found in the seeds of many common legumes and is known to inhibit protein synthesis and growth in a number of organisms. Yet canavanine may comprise as much as 4% of the seed dry weight of the jackbean (Canavalia ensiformis).Canavanine is accumulated during earlier development in the...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387456
更新日期:1967-06-01 00:00:00
abstract::Pea plants grown under different conditions of cultivation, and eight different plant species with variegated leaves were used to study the intracellular localization of shikimate oxidoreductase (EC 1.1.1.25), the marker enzyme of the pre-chorismate pathway. The two series of experiments indicated an intra-and an extr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00397408
更新日期:1983-07-01 00:00:00
abstract::Gibberellic acid (GA3) enhances ultrastructural morphogenesis of plastids in greening cereals whilst abscisic acid (ABA) and CCC have the reverse effect over a shorter period. GA3 application counteracts the ABA and CCC inhibition of membrane development and, over longer periods of greening, the fastest rate of chloro...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00385553
更新日期:1973-12-01 00:00:00
abstract::A β-amylase (EC 3.2.1.2) was identified in the outer pericarp (βP) of developing seeds of wheat (Triticum aestivum L.) and compared with the well known β-amylase which is synthesized during seed development in the starchy endosperm (βE). The enzyme βP already exists in the tissues before anthesis and vanishes at the t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00193003
更新日期:1990-07-01 00:00:00
abstract::The giant cells of the petiolar hairs of Heracleum mantegazzianum contain cytoplasmic fibres that are readily visible with phase contrast or interference contrast optics. These fibres are associated with saltatory particle movement and protoplasmic streaming in this cell and are believed to be the living image of simi...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386612
更新日期:1970-03-01 00:00:00
abstract::In maize and other grasses there is a developmental gradient from the meristematic cells at the base of the stalk to the differentiated cells at the leaf tip. This gradient presents an opportunity to investigate changes in mitochondrial DNA (mtDNA) that accompany growth under light and dark conditions, as done previou...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-012-1802-z
更新日期:2013-02-01 00:00:00
abstract:MAIN CONCLUSION:This review highlights 50 years of research on the fungal diterpene fusicoccin, during which the molecule went from a tool in plant physiology research to a pharmacological agent in treating animal diseases. Fusicoccin is a phytotoxic glycosylated diterpene produced by the fungus Phomopsis amygdali, a p...
journal_title:Planta
pub_type: 杂志文章,评审
doi:10.1007/s00425-018-3051-2
更新日期:2019-01-01 00:00:00
abstract::Synthesis of anthocyanin pigments in etiolated cabbage seedlings is influenced by ethylene at concentrations higher than 10 ppb, and etiolated seedlings produce sufficient ethylene to influence their anthocyanin synthesis. When escape of endogenous ethylene from this tissue is enhanced by means of hypobaric treatment,...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387635
更新日期:1973-09-01 00:00:00
abstract::Under long hydroxyurea treatments, evidence was obtained for the sequential activation of four checkpoints located between the onset of S phase and mitosis in Allium cepa L. root meristems. Biparametric flow cytometry (Br-DNA/total DNA) showed that cells initially accumulated at early S phase but, after a delay, they ...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250000415
更新日期:2001-02-01 00:00:00
abstract::Three cDNA clones encoding lipid transfer proteins (LTPs) were isolated by applying the rapid amplification of cDNA ends (RACE) protocol to imbibed seeds and germinating seedlings Brassica napus. The deduced amino-acid sequences show a great degree of homology and they exhibit the common features shared by all LTPs. T...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00196563
更新日期:1996-01-01 00:00:00
abstract::The cell-wall formation in the egg of Pelvetia fastigiata (J.G. Agardh) DeToni (Fucaceae) was studied with freeze-fracture. 1. The wall is lamellated with microfibrils approximately parallel in each lamella. The average orientation of microfibrils turns about 35° in each subsequent lamella. This slow turn gives rise t...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00386007
更新日期:1976-01-01 00:00:00
abstract::Conjugation was studied in Schizosaccharomyces pombe using liquid media. Nitrogen, which was growth-limiting in a synthetic medium, had to be consumed completely before conjugation could start. Conjugation was preceded by sexual agglutination. Agglutinability was not constitutive in heterothallic strains. It only deve...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00387025
更新日期:1971-03-01 00:00:00
abstract::Past genetic studies have shown three independent loci designated O, R and M control spathe color in Anthurium andraeanum (Hort.). To evaluate the genetic model and to understand the control of anthocyanin biosynthesis at the molecular level, the expression of the anthocyanin biosynthetic genes, CHS, F3H, DFR, ANS and...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-013-2007-9
更新日期:2014-03-01 00:00:00
abstract::The effects of (+)-8',8',8'-trifluoroabscisic acid (trifluoro-ABA) on alpha-amylase expression were studied in rice embryoless half-seeds, scutella, and suspension-cultured cells derived from the embryo, and the effects of the analog on sugar accumulation were also studied in scutella and suspension-cultured cells. Tr...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s004250050326
更新日期:1998-07-01 00:00:00
abstract::Glycosylation of sterols, catalysed by sterol glycosyltransferases (SGTs), improves the sterol solubility, chemical stability and compartmentalization, and helps plants to adapt to environmental changes. The SGTs in medicinal plants are of particular interest for their role in the biosynthesis of pharmacologically act...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-014-2046-x
更新日期:2014-06-01 00:00:00
abstract::Tobacco mesophyll protoplasts cultivated in vitro do not synthesize a measurable quantity of chloroplastic ribosomal RNA, but actively synthesize cytoplasmic ribosomal RNA, polyadenylated RNA, and proteins. These syntheses are essentially independent of the presence of hormones in the culture medium and are thus relat...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00384976
更新日期:1981-05-01 00:00:00
abstract:MAIN CONCLUSION:In this genome-wide association study, we obtained novel insights into the genetic basis of the effect of herbivory or drought stress on the level of resistance against the fungus Botrytis cinerea. In nature, plants function in complex environments where they encounter different biotic and abiotic stres...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-018-3065-9
更新日期:2019-04-01 00:00:00
abstract:MAIN CONCLUSION:Bignoniaceae species have conserved chloroplast structure, with hotspots of nucleotide diversity. Several genes are under positive selection, and can be targets for evolutionary studies. Bignoniaceae is one of the most species-rich family of woody plants in Neotropical seasonally dry forests. Here we re...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/s00425-020-03498-9
更新日期:2020-10-24 00:00:00
abstract::Several lines of evidence indicate that the conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by microsomal membranes from carnation flowers is attributable to hydroperoxides generated by membrane-associated lipoxygenase (EC 1.13.11.12). As the flowers senesce, the capability of isolated microsomal...
journal_title:Planta
pub_type: 杂志文章
doi:10.1007/BF00391036
更新日期:1985-05-01 00:00:00