Abstract：
:In situ lipolysis and synchrotron small-angle X-ray scattering (SAXS) were used to directly detect and elucidate the solid-state form of precipitated fenofibrate from the digestion of a model lipid-based formulation (LBF). This method was developed in light of recent findings that indicate variability in solid-state form upon the precipitation of some drugs during the digestion of LBFs, addressing the need to establish a real-time technique that enables solid-state analysis during in vitro digestion. In addition, an ex situ method was also used to analyse the pellet phase formed during an in vitro lipolysis experiment at various time points for the presence of crystalline drug. Fenofibrate was shown to precipitate in its thermodynamically stable crystalline form upon digestion of the medium-chain LBF, and an increase in scattering intensity over time corresponded well to an increase in concentration of precipitated fenofibrate quantified from the pellet phase using high-performance liquid chromatography. Crossed polarized light microscopy served as a secondary technique confirming the crystallinity of the precipitated fenofibrate. Future application of in situ lipolysis and SAXS may focus on drugs, and experimental conditions, which are anticipated to produce altered solid-state forms upon the precipitation of drug (i.e., polymorphs, amorphous forms, and salts).
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Khan J,Hawley A,Rades T,Boyd BJdoi
10.1002/jps.24634subject
Has Abstractpub_date
2016-09-01 00:00:00pages
2631-2639issue
9eissn
0022-3549issn
1520-6017pii
S0022-3549(15)00066-0journal_volume
105pub_type
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journal_title：Journal of pharmaceutical sciences
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journal_title：Journal of pharmaceutical sciences
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