High Glucose Concentrations Suppress the Proliferation of Human Periodontal Ligament Stem Cells and Their Differentiation Into Osteoblasts.

Abstract:

BACKGROUND:Diabetes mellitus (DM) is a major risk factor for periodontal disease and affects various cellular functions. Periodontal ligament stem cells (PDLSCs) play an important role in periodontal tissue regeneration; however, the effect of hyperglycemia on PDLSCs is unclear. The aim of this study is to investigate whether hyperglycemia affects periodontal tissue regeneration, using human PDLSCs and high-glucose medium as a model of DM. METHODS:PDLSCs were obtained from healthy adult human mandibular third molars. Cell proliferation, osteoblastic differentiation, and proinflammatory cytokine expression were investigated by culturing PDLSCs in media supplemented with four different glucose concentrations representative of control patients (5.5 mM), patients with postprandial or controlled DM (8.0 mM), and patients with uncontrolled DM (12.0 and 24.0 mM). The molecular effects of hyperglycemia on PDLSC physiology were examined with a focus on the nuclear factor (NF)-(κB signaling pathway. The involvement of NF-κB was investigated with a specific NF-κB inhibitor in PDLSCs under hyperglycemic conditions. RESULTS:High glucose levels inhibited PDLSC proliferation and differentiation into osteoblasts but induced NF-κB activation and subsequent interleukin (IL)-6 and IL-8 expression. Treatment with an NF-κB inhibitor rescued the defects in cell proliferation and osteoblastic differentiation and inhibited the IL-6 expression caused by the high-glucose environment. CONCLUSION:The results of this study demonstrate that hyperglycemia inhibits human PDLSC proliferation and osteoblastic differentiation.

journal_name

J Periodontol

authors

Kato H,Taguchi Y,Tominaga K,Kimura D,Yamawaki I,Noguchi M,Yamauchi N,Tamura I,Tanaka A,Umeda M

doi

10.1902/jop.2015.150474

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

e44-51

issue

4

eissn

0022-3492

issn

1943-3670

journal_volume

87

pub_type

杂志文章
  • Effects of occlusal overload on peri-implant tissue health: a systematic review of animal-model studies.

    abstract:BACKGROUND:This study systematically evaluates the effect of occlusal overload (OV) on peri-implant tissue health in animal studies. METHODS:MEDLINE, EMBASE, and LILACS databases were searched for articles published up to and including January 2010. Studies that reported outcomes of non-splinted titanium dental implan...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,评审

    doi:10.1902/jop.2010.100176

    authors: Chambrone L,Chambrone LA,Lima LA

    更新日期:2010-10-01 00:00:00

  • Altered cell motility and attachment with titanium surface modifications.

    abstract:BACKGROUND:Titanium implants are widely used in dentistry to replace lost teeth. Various surface modifications have been used to improve implant retention and osseointegration. This study is designed to compare the ability of three titanium surfaces to promote cell attachment and cell motility of cells relevant to peri...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2011.100733

    authors: Palaiologou A,Stoute D,Fan Y,Lallier TE

    更新日期:2012-01-01 00:00:00

  • Fiber glass splints.

    abstract::Temporary splints to immobilize periodontally diseased teeth were made by fiber glass bonded and attached to the teeth with acid-etch technique with BIS-GMA resin. Twenty-one splints were made. Observation time ranged from 4 to 14 months. During the observation only one splint fractured and was repaired while the othe...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1979.50.4.193

    authors: Friskopp J,Blomlöf L,Söder PO

    更新日期:1979-04-01 00:00:00

  • Effect of restraint stress on the progression of experimental periodontitis in rats.

    abstract:BACKGROUND:A hypothesis of an increased risk for destructive periodontal diseases due to psychological stress has long been promoted. However, the research on stress and periodontal disease is still in its infancy. One of the reasons is thought to be that there is no suitable animal model for investigating the relation...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2004.75.2.306

    authors: Takada T,Yoshinari N,Sugiishi S,Kawase H,Yamane T,Noguchi T

    更新日期:2004-02-01 00:00:00

  • Histopathological investigation of gingival tissue from patients with rapidly progressive periodontitis.

    abstract::In this study, fine structural features of the pocket walls in rapidly progressive periodontitis (RPP) and adult periodontitis (AP) in 20 cases were compared using light and transmission electron microscopy. Gingiva was also obtained from a control group of periodontally healthy teeth. Clinical parameters were assesse...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1998.69.2.195

    authors: Hillmann G,Dogan S,Geurtsen W

    更新日期:1998-02-01 00:00:00

  • Effective periodontal treatment in a patient with type IIA von Willebrand's disease: report of a case.

    abstract::von Willebrand's disease (vWD) is one of the most common hereditary hemorrhagic disorders. A mild to moderate deficiency of factor VIII and von Willebrand factor (vWf) often is associated with gingival bleeding. In this case report, the periodontal treatment of a patient with vWD is described. A 45-year-old woman with...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1999.70.5.548

    authors: Izumi Y,Taniguchi T,Maruyama Y,Sueda T

    更新日期:1999-05-01 00:00:00

  • Boric acid irrigation as an adjunct to mechanical periodontal therapy in patients with chronic periodontitis: a randomized clinical trial.

    abstract:BACKGROUND:The purpose of this single-masked, randomized, controlled clinical trial was to evaluate the effects of boric acid irrigation as an adjunct to scaling and root planing (SRP) on clinical and microbiologic parameters and compare this method with chlorhexidine irrigation and SRP alone in patients with chronic p...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,随机对照试验

    doi:10.1902/jop.2012.120467

    authors: Sağlam M,Arslan U,Buket Bozkurt Ş,Hakki SS

    更新日期:2013-09-01 00:00:00

  • Oral food consumption and subgingival microorganisms: subgingival microbiota of gastrostomy tube-fed children and healthy controls.

    abstract::This study examined the effect of oral food consumption on the prevalence and levels of subgingival bacteria and yeasts in 20 gastrostomy tube-fed children and 24 healthy controls. Microbial identification was carried out using anaerobic culture and 16S rRNA-based PCR identification methods. Streptococcal and Actinomy...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1997.68.12.1163

    authors: Chen C,Ashimoto A,Sangsurasak S,Flynn MJ,Slots J

    更新日期:1997-12-01 00:00:00

  • Spontaneous pigmentation of non-pigmented palatal tissue after periodontal surgery.

    abstract:BACKGROUND:A 22-year-old African American female was referred for augmentation of keratinized gingiva around implants at the right and left maxillary second premolar sites. Presurgical evaluation revealed generalized melanosis of the buccal gingiva and a lack of keratinized tissue around implants at sites #4 and #13. N...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2009.090289

    authors: Holtzclaw D,Toscano NJ,Tal H

    更新日期:2010-01-01 00:00:00

  • A new hypothesis of periodontal homeostasis.

    abstract::A review of the experimental evidence evidence on marrow inhibition by implanted roots has shown that an inhibitory factor is present in all root surfaces. It is most marked in periodontally diseased roots and least in periodontally healthy roots. It is hypothesized that a "marked" amount would inhibit periodontal cel...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1980.51.1.49

    authors: Morris ML

    更新日期:1980-01-01 00:00:00

  • Treatment of dehiscence-type defects with collagen matrix and/or enamel matrix derivative: Histomorphometric study in minipigs.

    abstract:BACKGROUND:This study aimed to evaluate, histomorphometrically, the use of collagen matrix (CM) and/or enamel matrix derivative (EMD) for the treatment of dehiscence-type recession defects in minipigs. METHODS:Eight healthy, male, young BR-1 minipigs, with no periodontal disease were treated. Bilateral dehiscence-type...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1002/JPER.19-0107

    authors: França-Grohmann IL,Sangiorgio JPM,Bueno MR,Casarin RCV,Silvério Ruiz KG,Nociti FH Jr,Casati MZ,Sallum EA

    更新日期:2020-07-01 00:00:00

  • Early periodontal disease in the Syrian hamster.

    abstract::An investigation into periodontal disease in the Syrian Golden hamster was conducted over a period of six months. With the periodontitis-producing Diet 2000 only mild destruction of the periodontium was observed. The interproximal area between M1 and M2 may be a better area for analysis of early periodontal disease in...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1975.46.6.368

    authors: Miller WA,Ripley JF

    更新日期:1975-06-01 00:00:00

  • Periodontal status and serum antibody responses to oral microorganisms in Sjögren's syndrome.

    abstract::Sjögren's syndrome is an autoimmune disease characterized by keratoconjunctivitis sicca and xerostomia. Rapid bacterial plaque accumulation occurs in Sjögren's syndrome patients due to decreases in salivary flow rate. The purpose of this study was to evaluate the periodontal status of patients with Sjögren's syndrome ...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1998.69.5.571

    authors: Celenligil H,Eratalay K,Kansu E,Ebersole JL

    更新日期:1998-05-01 00:00:00

  • Systematic errors in estimating prevalence and severity of periodontal disease.

    abstract::Partial-mouth scores are often used in epidemiologic studies to estimate the prevalence and severity of periodontal disease. Such estimates systematically underestimate the prevalence of disease, but the bias can be in either direction for disease severity. Three large data sets containing full-mouth examinations (mes...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1988.59.11.707

    authors: Kingman A,Morrison E,Löe H,Smith J

    更新日期:1988-11-01 00:00:00

  • Bacterial invasion in experimental gingivitis in man.

    abstract::Gingival biopsies of the mesial papilla area of the first molar were obtained from each patient at 0-, 14- and 21-day intervals during plaque formation. The biopsies were fixed, serially sectioned, and Gram-stained. The incidence and distribution of the bacteria-like structures were studied by microscopy. In all the s...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1987.58.12.837

    authors: Saglie FR,Pertuiset JH,Rezende MT,Sabet MS,Raoufi D,Carranza FA Jr

    更新日期:1987-12-01 00:00:00

  • The influence of interleukin gene polymorphism on expression of interleukin-1beta and tumor necrosis factor-alpha in periodontal tissue and gingival crevicular fluid.

    abstract:BACKGROUND:A specific composite genotype of the polymorphic interleukin-1 (IL-1) gene cluster has recently been associated with severe periodontitis. One polymorphism of the composite periodontitis-associated genotype (PAG) has been functionally linked with expression of high levels of IL-1. The purpose of this study w...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1999.70.6.567

    authors: Engebretson SP,Lamster IB,Herrera-Abreu M,Celenti RS,Timms JM,Chaudhary AG,di Giovine FS,Kornman KS

    更新日期:1999-06-01 00:00:00

  • Phenytoin-induced gingival overgrowth resulting in delayed eruption of the primary dentition. A case report.

    abstract::A case of delayed eruption of the primary dentition secondary to phenytoin therapy in a 4-year-old child is reported. The patient had been on phenytoin therapy since the first months of life. Radiographs revealed that alveolar but not gingival emergence had occurred. Surgical excision of the excess gingival tissue was...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1984.55.1.19

    authors: Church LF Jr,Brandt SK

    更新日期:1984-01-01 00:00:00

  • Distribution and readability of periodontal health education literature.

    abstract::THIS INVESTIGATION was undertaken to determine the distribution pattern and readability level of 20 widely distributed printed items containing periodontal health education literature. Average annual distribution was obtained from distributors of the material. The Minnesota Interactive Readability Approximation Progra...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1983.54.9.538

    authors: Bakdash MB,Odman PA,Lange AL

    更新日期:1983-09-01 00:00:00

  • Osteopromotion: a soft-tissue exclusion principle using a membrane for bone healing and bone neogenesis.

    abstract::The research reviewed in this paper constitutes a series of investigations intended to develop and evaluate a new membrane technique, which provides improved conditions for osteogenesis during healing of bone defects and restitution of earlier existing bone. The technique has also been shown to aid in bone grafting as...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,评审

    doi:10.1902/jop.1993.64.11s.1116

    authors: Linde A,Alberius P,Dahlin C,Bjurstam K,Sundin Y

    更新日期:1993-11-01 00:00:00

  • Oral manifestations of hereditary hemorrhagic telangiectasia (Osler-Weber-Rendu disease). Review and case reports.

    abstract::A review of hereditary hemorrhagic telangiectasia (Osler-Weber-Rendu Disease) is presented. Special emphasis is placed on the oral manifestations which include hemorrhagic vesicles, nodules, papules and ulcers that involve the gingiva and other areas of the mucosa. Two cases are included which were seen in a periodont...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1982.53.3.163

    authors: Bartolucci EG,Swan RH,Hurt WC

    更新日期:1982-03-01 00:00:00

  • Laser irradiation of bone: III. Long-term healing following treatment by CO2 and Nd:YAG lasers.

    abstract:BACKGROUND:Relative few reports exist concerning healing of laser created osteotomies over an extended period of time. The purpose of this study was to evaluate long-term healing, from 21 to 63 days, of osteotomy defects in the rat tibia created with the Nd:YAG and CO2 in the presence of a surface cooling spray of air/...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2001.72.2.174

    authors: McDavid VG,Cobb CM,Rapley JW,Glaros AG,Spencer P

    更新日期:2001-02-01 00:00:00

  • Early soft tissue healing around one-stage dental implants: clinical and microbiologic parameters.

    abstract:BACKGROUND:Despite the potential significance of early soft tissue healing to long-term outcomes, this aspect of one-stage dental implants has not been investigated. The purpose of this prospective study was to characterize clinical and microbiologic parameters of early soft tissue healing around dental implants placed...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2007.070122

    authors: DeAngelo SJ,Kumar PS,Beck FM,Tatakis DN,Leblebicioglu B

    更新日期:2007-10-01 00:00:00

  • Increased Interleukin-1β Concentration in Gingival Crevicular Fluid as a Characteristic of Periodontitis.

    abstract:BACKGROUND:The levels of interleukin-1β (IL-1β) have been reported to be higher in sites with periodontitis than in healthy controls. This may be the result of a more severe inflammation and/or constitutional differences in IL-1β production. Our aim was to test the hypothesis that the level of IL-1β in gingival crevicu...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1999.70.12.1457

    authors: Figueredo CMS,Ribeiro MSM,Fischer RG,Gustafsson A

    更新日期:1999-12-01 00:00:00

  • Surgical versus non-surgical periodontal treatment: psychosocial factors and treatment outcomes.

    abstract:BACKGROUND:This research explored how patients with surgical versus non-surgical periodontal treatment differ in trait anxiety, depression, perceived stress, and well-being on the day of surgery and in their reported pain and use of pain medication 2 and 4 weeks after treatment. In addition, it was investigated how psy...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2006.050302

    authors: Kloostra PW,Eber RM,Wang HL,Inglehart MR

    更新日期:2006-07-01 00:00:00

  • Human periodontal ligament cell responses to recombinant human bone morphogenetic protein-2 with and without bone allografts.

    abstract:BACKGROUND:Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been found to promote the osteoblastic differentiation of human periodontal ligament cells. Its effect depends on the delivery system used. In this study we examined the effect of rhBMP-2 on the proliferation and osteoblastic differentiation of hum...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2003.74.7.982

    authors: Markopoulou CE,Vrotsos IA,Vavouraki HN,Dereka XE,Mantzavinos ZS

    更新日期:2003-07-01 00:00:00

  • Healing responses of human intraosseous lesions following the use of debridement, grafting and citric acid root treatment. I. Clinical and histologic observations six months postsurgery.

    abstract::Three block sections of treated human intraosseous lesions within one individual are presented. These lesions were debrided, filled with autogenous grafts and at two sites the root was treated with citric acid. The treated sites were lightly root planed once a month and the blocks were removed 6 months after surgery. ...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1983.54.2.67

    authors: Froum SJ,Kushner L,Stahl SS

    更新日期:1983-02-01 00:00:00

  • Clinical evaluation of acellular allograft dermis for the treatment of human gingival recession.

    abstract:BACKGROUND:Periodontal root coverage procedures to treat recession areas are indicated for unesthetic, exposed, and/or painful root surfaces. Many methods, most using autogenous soft tissue grafts, have been utilized, but with associated morbidity at the donor sites. An alternative donor material would reduce the morbi...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章

    doi:10.1902/jop.2001.72.8.998

    authors: Aichelmann-Reidy ME,Yukna RA,Evans GH,Nasr HF,Mayer ET

    更新日期:2001-08-01 00:00:00

  • A comparison of the efficacy of 2 powered toothbrushes in affecting plaque accumulation, gingivitis, and gingival bleeding.

    abstract:BACKGROUND:The purpose of this 30-day blinded, parallel-design study was to compare the effect of 2 powered toothbrushes, the Rowenta MH700 and the Braun Plak Control Ultra, on reducing plaque accumulation, gingivitis, and gingival bleeding in a cohort of 60 healthy adults. METHODS:After baseline evaluation of plaque,...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1902/jop.1999.70.8.840

    authors: Barnes CM,Russell CM,Weatherford TW 3rd

    更新日期:1999-08-01 00:00:00

  • Buccal bone thickness adjacent to virtual dental implants following guided bone regeneration.

    abstract:BACKGROUND:Crestal bone thickness is a critical determinant of peri-implant tissue stability. This retrospective observational study sought to quantify the buccal bone thickness achieved adjacent to virtual dental implants following guided bone regeneration (GBR) surgery and evaluate the influence of patient- and proce...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1002/JPER.18-0304

    authors: Phillips DJ,Swenson DT,Johnson TM

    更新日期:2019-06-01 00:00:00

  • Differential effects of amelogenin on mineralization of cementoblasts and periodontal ligament cells.

    abstract:BACKGROUND:Amelogenin is a major component of developing extracellular enamel matrix proteins and plays a crucial role during the formation of tooth enamel. In addition, amelogenins are suggested to exert biologic functions as signaling molecules through cell-surface receptors. The purpose of this study is to examine t...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2011.110408

    authors: Tanimoto K,Kunimatsu R,Tanne Y,Huang YC,Michida M,Yoshimi Y,Miyauchi M,Takata T,Tanne K

    更新日期:2012-05-01 00:00:00