Abstract:
BACKGROUND:Amelogenin is a major component of developing extracellular enamel matrix proteins and plays a crucial role during the formation of tooth enamel. In addition, amelogenins are suggested to exert biologic functions as signaling molecules through cell-surface receptors. The purpose of this study is to examine the effect of recombinant human full-length amelogenin (rh174) on the mineralization of human cementoblasts (HCEMs) and human periodontal ligament cells (HPDLs). METHODS:HCEMs, namely, a cell line immortalized by transfection of human telomerase reverse transcription gene, and HPDLs isolated from human first premolars were cultured and treated with 0 to 1,000 ng/mL rh174. The messenger ribonucleic acid (mRNA) levels of alkaline phosphatase (ALP), osteocalcin (OCN), and bone sialoprotein (BSP) were examined by real-time polymerase chain reaction analysis. The protein levels of OCN and BSP were examined by Western blot analysis. ALP activity and calcium deposition of cell cultures were also determined. Mineralization of cells was evaluated by red dye staining. RESULTS:The treatment of HCEMs with rh174 upregulated the ALP, OCN, and BSP mRNA levels. In addition, the protein levels of OCN and BSP, ALP activity, and calcium deposition were enhanced, resulting in enhanced mineralization. Conversely, there were no significant effects of rh174 on the mineralization of HPDLs. CONCLUSION:The present study shows that rh174 enhances mineralization accompanied by upregulation of mineralization markers in HCEMs, whereas it has no effect on that in HPDLs, suggesting different effects of amelogenin on PDL and cementum.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Tanimoto K,Kunimatsu R,Tanne Y,Huang YC,Michida M,Yoshimi Y,Miyauchi M,Takata T,Tanne Kdoi
10.1902/jop.2011.110408subject
Has Abstractpub_date
2012-05-01 00:00:00pages
672-9issue
5eissn
0022-3492issn
1943-3670journal_volume
83pub_type
杂志文章abstract::Periodontal disease is a common infection-induced inflammatory disease among individuals suffering from diabetes mellitus. The purpose of this study was to assess the effects of treatment of periodontal disease on the level of metabolic control of diabetes. A total of 113 Native Americans (81 females and 32 males) suf...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.1997.68.8.713
更新日期:1997-08-01 00:00:00
abstract:BACKGROUND:Areca quid chewers have a higher prevalence of periodontal disease than non-chewers. Little is known about the influence of areca quid on the immune system. This study was to determine the possible effects of the areca nut on phagocytic activity of human neutrophils. METHODS:Aqueous extracts of ripe areca n...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2005.76.3.373
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:Crohn's disease is a chronic inflammatory bowel disease characterized by uncertainty in etiology and pathogenesis occasionally with manifestations in oral mucous membranes. This report reviews the literature on Crohn's disease and presents a patient with Crohn's disease on continuous anti-inflammatory and im...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.2003.74.4.537
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:There is a large body of evidence using cells and animal models demonstrating the effectiveness of growth factors in periodontal regeneration. However, there appears to be minimal literature comparing the efficacy of growth factors in human periodontal regeneration compared to other techniques and procedures...
journal_title:Journal of periodontology
pub_type: 杂志文章,meta分析,评审
doi:10.1902/jop.2012.120145
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:Reports from studies of twins, disease aggregation in families, animal models for periodontal disease, and various genetic-analysis studies have determined that genetics plays a role in the susceptibility to periodontal disease. The purpose of this pilot study is to evaluate the effect of genetics on periodo...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2010.100189
更新日期:2011-04-01 00:00:00
abstract::The interpretation of dental radiographs for the diagnosis of periodontal disease conditions poses several difficulties. These include the inability to adequately reproduce the projection geometry and optical density of the exposures. In order to improve the ability to extract accurate quantitative information from a ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1983.54.7.435
更新日期:1983-07-01 00:00:00
abstract:BACKGROUND:Non-surgical periodontal treatment with an Er:YAG laser has been shown to result in significant clinical attachment level gain; however, clinical results have not been established on a long-term basis following Er:YAG laser treatment. Therefore, the aim of the present study was to present the 2-year results ...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.2003.74.5.590
更新日期:2003-05-01 00:00:00
abstract::To date, there are no ultrastructural studies that have examined untreated chronic periodontal pockets immediately following dental debridement with an oral irrigation device. This study used both scanning electron microscopic and transmission electron microscopic methodology to examine previously untreated human peri...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1988.59.3.155
更新日期:1988-03-01 00:00:00
abstract::The analgesic activity of single doses of meclofenamate (100 mg) was compared to aspirin (500 mg) and to placebo on 99 outpatients with moderate to severe pain following periodontal surgery under double-blind conditions. Pain intensity differences scores (PID) at the first, second, and third hour after the ingestion o...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
doi:10.1902/jop.1992.63.3.166
更新日期:1992-03-01 00:00:00
abstract::Subtraction radiography requires radiographs made with identical projection geometry. The purpose of the present investigation was to develop and evaluate a new in vivo method to measure angular radiographic projection error. This included the development of a system for accurate control of the x-ray beam angulation r...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1991.62.11.674
更新日期:1991-11-01 00:00:00
abstract::A review of the 2006 and past American Dental Association (ADA) studies on the distribution of dentists provides information on the evolving number of periodontists. Despite an overall increase in the number of periodontists, major differences continue in practitioner/population ratios in different geographic areas. A...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080592
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate the efficacy of deproteinized bovine bone mineral with 10% collagen (DBBM-C) soaked with hyaluronic acid (HA) for ridge preservation in compromised extraction sockets. METHODS:Bilateral third, fourth premolars and first molar were hemisected, distal roots were extracted...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.20-0832
更新日期:2021-01-23 00:00:00
abstract:BACKGROUND:Neopterin, a marker of cellular immune activation, is produced by human macrophages after induction by interferon gamma that is secreted by T lymphocytes. Neopterin concentrations in diverse body fluids have been reported to increase in parallel with bacteria in the clinical course of infections. Therefore, ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2002.73.7.720
更新日期:2002-07-01 00:00:00
abstract::The purpose of this study was to evaluate the effects of the Nd:YAG laser on in vitro fibroblast attachment to endotoxin-treated root surfaces and to describe any laser-induced cementum surface alterations. Thirty 4 mm x 4 mm cementum segments were obtained from unerupted third molars. The treatment groups were as fol...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1992.63.7.626
更新日期:1992-07-01 00:00:00
abstract::The authors review patient-reported outcome (PRO) metrics for dentistry, and in particular, periodontics. The PRO commentary for periodontics includes a review of split-mouth, randomized, controlled clinical trial results that specifically tracked pain at different sites over time after intervention and provided guide...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.2014.130693
更新日期:2014-10-01 00:00:00
abstract::Few scientific investigations have addressed the ability of mouthrinses to reduce oral malodor for periods longer than 3 hours. In the present report, we have employed simple, recently described techniques to assess the day-long reduction in oral malodor of a novel 2-phase oil:water mouthrinse (TPM), as compared to a ...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.1992.63.1.39
更新日期:1992-01-01 00:00:00
abstract::Chlorhexidine mouthrinse is a widely used adjunct in periodontal therapy due to its bactericidal effects. The effect of this agent on chronic gingivitis and wound healing following surgical therapy in animals and humans has been favorable. The re-establishment of lost connective tissue attachment to the root surface f...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1991.62.7.434
更新日期:1991-07-01 00:00:00
abstract::Cyclosporine A (CsA) and verapamil are two agents used in renal transplantation, both of which are suspected of inducing gingival overgrowth. This study was conducted to investigate the effect of verapamil on the severity and prevalence of CsA-induced gingival overgrowth. Fifty-one (51) renal transplant recipients (to...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1996.67.11.1201
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:The levels of interleukin-1β (IL-1β) have been reported to be higher in sites with periodontitis than in healthy controls. This may be the result of a more severe inflammation and/or constitutional differences in IL-1β production. Our aim was to test the hypothesis that the level of IL-1β in gingival crevicu...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1999.70.12.1457
更新日期:1999-12-01 00:00:00
abstract:BACKGROUND:Periodontitis patients harboring Actinobacillus actinmycetemcomitans (Aa) are prime candidates for systemic antibiotic therapy. Besides tetracycline and the combination of metronidazole and amoxicillin the fluoroquinolones are also believed to have antibacterial activity against Aa. The aim of the present st...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
doi:10.1902/jop.2000.71.2.202
更新日期:2000-02-01 00:00:00
abstract:BACKGROUND:Periodontal disease is a major complication of type 1 diabetes mellitus (T1DM). The aim of the present study is to investigate the relationship between glycated hemoglobin and circulating levels of interleukin (IL)-6, IL-8, and C-X-C motif chemokine ligand 5 (CXCL5) in non-smoking patients suffering from T1D...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2015.150149
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:This updated Cochrane systematic review (SR) evaluated the efficacy of different root coverage (RC) procedures in the treatment of single and multiple gingival recessions (GR). METHODS:We included randomized controlled trials (RCTs) only of at least 6 months' duration evaluating Miller's Class I or II GR (≥...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.19-0079
更新日期:2019-12-01 00:00:00
abstract::Greater cell attachment to demineralized dentin has been associated with subsequent development of a fiber attachment system. The purpose of this study was to evaluate tissue interactions to dentin demineralized with different concentrations of tetracycline solution. Dentin specimens were obtained from beneath root su...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1988.59.11.714
更新日期:1988-11-01 00:00:00
abstract::Invasion of gingival tissues by bacteria is thought to be a major factor in development of periodontal lesions. Morphologic studies have revealed bacteria within the pocket epithelium, gingival connective tissues, alveolar bone, and oral epithelium. The current studies are intended to determine whether they are presen...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1990.61.1.35
更新日期:1990-01-01 00:00:00
abstract::The use of antibiotics in periodontal therapy has received increasing attention in recent years. Although generally safe, certain antibiotics, including tetracycline and ampicillin, have been shown to interact indirectly with oral contraceptives, with a resultant decrease in oral contraceptive efficacy. This paper rev...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1985.56.1.18
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND:This preliminary in vitro study, presented at the General Session of the International and American Association for Dental Research in 2010 (Barcelona, Spain), evaluates whether three-dimensional (3D) optical scanning and subsequent superimposing procedures could be used for reproducible volumetric evaluatio...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2011.110143
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Autoinducer (AI)-2 has an important role in biofilm formation in the oral environment. Mature biofilms formed as a result of the cell-to-cell communication make it difficult to overcome periodontitis with the use of antibiotics. Previous in vitro studies suggest that quorum-sensing inhibitors (QSIs) interfer...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2016.160070
更新日期:2016-09-01 00:00:00
abstract::Studies of the inductive properties of human dentin and cementum have yielded differing results. Comparisons are difficult to draw because of variations in time, donor tissue, host immunologic status, implant site, decalcifying agents and degree of decalcification. Analysis of the many experiments intimates that the a...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1985.56.11.699
更新日期:1985-11-01 00:00:00
abstract::Oral malodor was measured using a portable sulphide monitor in 2,672 individuals aged 18 to 64 years. In addition, dental (DMFT) and periodontal conditions (CPITN and attachment loss), dental plaque, and tongue coating status were assessed. Before clinical examination, subjects were interviewed about their oral health...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1995.66.8.679
更新日期:1995-08-01 00:00:00
abstract::T-cell subsets extracted from chronically inflamed periodontal tissues were identified using monoclonal antibodies, and their functional activity was analysed using the autologous mixed lymphocyte reaction (AMLR). Tissue was obtained from a total of 33 adult periodontitis (AP) patients and 6 normal/marginal gingivitis...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1987.58.8.569
更新日期:1987-08-01 00:00:00