Abstract：
:It is commonly thought that central nervous system depressant drugs exert their actions through enhancement of gamma-aminobutyrate (GABA)-mediated mechanisms. Recently, the cellular electrophysiological evidence from this laboratory and others suggests that both sedative hypnotics and general anaesthetics inhibit central neurons by increasing potassium conductance (GK). We have utilized the mammalian in vitro hippocampal and cerebellar slice preparations at 34-36 degrees C. Intracellular recordings from CA1, CA3, and cerebellar Purkinje cells were obtained. Low dose (sedative) concentrations of ethanol (less than or equal to 20 mM), two different benzodiazepines (midazolam and clonazepam in low nanomolar concentrations), and pentobarbital (10(-6) to 10(-4) M) were applied by pressure ejection or were bath perfused. All drugs caused a hyperpolarization with decreased spontaneous activity, and enhanced post spike afterhyperpolarizations (AHPs). These long-lasting AHPs are presumably due to enhanced calcium-mediated GK. Increased responsiveness to focally applied GABA was only seen at higher doses (ethanol, 100 mM; midazolam, 10(-7) M; pentobarbital, 10(-4) M). These data suggest that the above neurodepressant drugs, when applied at sedative doses to hippocampal pyramidal cells, enhance GK and not the actions of GABA.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Carlen PL,Gurevich N,Davies MF,Blaxter TJ,O'Beirne Mdoi
10.1139/y85-137subject
Has Abstractpub_date
1985-07-01 00:00:00pages
831-7issue
7eissn
0008-4212issn
1205-7541journal_volume
63pub_type
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journal_title：Canadian journal of physiology and pharmacology
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pub_type： 杂志文章
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章
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更新日期：1987-03-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
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更新日期：2007-11-01 00:00:00
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更新日期：1987-04-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type：
doi：
更新日期：1996-03-01 00:00:00
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journal_title：Canadian journal of physiology and pharmacology
pub_type： 杂志文章,评审
doi：
更新日期：1997-05-01 00:00:00
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