Adjuvant activities of saponins from the root of Polygala senega L.

Abstract:

:Eight pure triterpenoid saponin compounds isolated from the root of Polygala senega L., a plant indigenous to the Canadian prairies, were evaluated for their immunological activity in mouse models. The specific antibody responses of the IgG2a subclass increased significantly when isolated P. senega saponins were used as adjuvants in the immunization of mice with OVA antigen. In addition, increased IL-2 levels were observed in spleen cell cultures from P. senega saponin-immunized mice after in vitro secondary antigen stimulation. The saponins were tested for their toxicity in mice by using a haemolytic activity assay and found to be less toxic than Quillaja saponaria saponins that have long been used as adjuvants in vaccine formulations. This study has shown the potential of P. senega saponins to be considered as a natural source of vaccine adjuvants with biological activity equivalent to the current commercially available saponin adjuvants.

authors

Katselis GS,Estrada A,Gorecki DK,Barl B

doi

10.1139/Y07-109

subject

Has Abstract

pub_date

2007-11-01 00:00:00

pages

1184-94

issue

11

eissn

0008-4212

issn

1205-7541

pii

y07-109

journal_volume

85

pub_type

杂志文章
  • Implication of multiple signaling pathways in the regulation of angiotensin II induced enhanced expression of Giα proteins in vascular smooth muscle cells.

    abstract::We have previously shown that A10 vascular smooth muscle cells (VSMC) exposed to angiotensin II (Ang II) exhibited overexpression of Giα proteins. In the present study, we examined the involvement of different signaling pathways in regulating Ang II induced enhanced expression of Giα proteins in VSMC by using pharmaco...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y2012-042

    authors: Li Y,Anand-Srivastava MB

    更新日期:2012-08-01 00:00:00

  • Involvement of insulin-like growth factor 1 receptor transactivation in endothelin-1-induced signaling in vascular smooth muscle cells.

    abstract::Endothelin-1 (ET-1) is a potent vasoactive peptide that exerts hypertrophic, migratory, and mitogenic effects in vascular smooth muscle cells. ET-1-induced activation of several signaling events has been shown to mediate the cellular effects of ET-1. In the past several years, transactivation of growth factor receptor...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/y10-030

    authors: Bouallegue A,Vardatsikos G,Srivastava AK

    更新日期:2010-05-01 00:00:00

  • The role of damage- and pathogen-associated molecular patterns in inflammation-mediated vulnerability of atherosclerotic plaques.

    abstract::Atherosclerosis is a chronic inflammatory disease resulting in the formation of the atherosclerotic plaque. Plaque formation starts with the inflammation in fatty streaks and progresses through atheroma, atheromatous plaque, and fibroatheroma leading to development of stable plaque. Hypercholesterolemia, dyslipidemia,...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/cjpp-2016-0664

    authors: Rai V,Agrawal DK

    更新日期:2017-10-01 00:00:00

  • Effect of lithium chloride on ornithine decarboxylase activity in rat adrenal.

    abstract::The effects of lithium chloride on ornithine decarboxylase (ODC) activity were compared in the adrenal and kidney of control (saline treated) and prolactin-treated rats. ODC activity was decreased in kidney of both groups of animals, the magnitude of the effect of lithium in the hormone-treated group varying with the ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y92-180

    authors: Strümpfer A,Hsiao J,Peng T,Richards J

    更新日期:1992-09-01 00:00:00

  • Distribution and metabolism of corticotropin-releasing factor in the rat.

    abstract::To develop a mathematical model of the distribution and metabolism of rat corticotropin-releasing factor (rCRF), the time course of 125I-labelled rCRF in plasma was measured in male Sprague-Dawley rats (i) following a rapid injection of 24 ng rCRF/100 g body weight (BW), or (ii) following a rapid injection of 424 ng r...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y86-113

    authors: Candas B,Lalonde J,Normand M

    更新日期:1986-06-01 00:00:00

  • Oxidative stress burden inhibits spermatogenesis in adult male rats: testosterone protective effect.

    abstract::In this study, we aimed to investigate the protective effects of androgens, using letrozole (LET; an aromatase inhibitor), grape seed extract (GSE; a naturally occurring aromatase inhibitor and antioxidant), and testosterone propionate (Tp), against methotrexate (MTX)-induced testicular toxicity in adult male rats. MT...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/cjpp-2017-0459

    authors: Makary S,Abdo M,Fekry E

    更新日期:2018-04-01 00:00:00

  • Channels activated by stretch in neurons of a helix snail.

    abstract::Single-channel recordings from central neurons of the helix snail, Cepaea nemoralis, revealed two types of channels that could be activated by stretch (i.e., by the membrane deformation produced when suction is applied to the patch pipette). One, a K+ channel (58 pS in physiological solution), was evident in excised a...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y92-025

    authors: Bédard E,Morris CE

    更新日期:1992-02-01 00:00:00

  • Protein tyrosine phosphorylation, cellular Ca2+, and Ca2+ sensitivity for contraction of smooth muscle.

    abstract::Our studies are guided by the novel hypothesis that protein tyrosine phosphorylation is an important mechanism for regulating contraction of smooth muscle. Several lines of evidence are reviewed which suggest that enhanced tyrosine phosphorylation participates in mechanisms that regulate cytosolic Ca2+ and Ca2+ sensit...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/y94-207

    authors: Di Salvo J,Pfitzer G,Semenchuk LA

    更新日期:1994-11-01 00:00:00

  • Local antinociceptive action of fluoxetine in the rat formalin assay: role of l-arginine/nitric oxide/cGMP/KATP channel pathway.

    abstract::The present study was conducted to evaluate the local antinociceptive actions of fluoxetine, a selective serotonin reuptake inhibitor, and the possible involvement of the l-arginine/NO/cGMP/KATP channel pathway in this effect using the formalin test in rats. To elucidate the underlying mechanisms, animals were pre-tre...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/cjpp-2017-0003

    authors: Ghorbanzadeh B,Mansouri MT,Naghizadeh B,Alboghobeish S

    更新日期:2018-02-01 00:00:00

  • The 1991 Borden Award Lecture. Selected aspects of intraluminal and intracellular phases of intestinal fat absorption.

    abstract::The recognition of chylomicrons as dietary lipid transporters dates back to more than 70 years and marks a milestone in lipoprotein history. Conventionally, three phases constitute the process of absorption of exogenous fat: intraluminal, intestinal, and delivery. The intraluminal phase includes chemical hydrolysis by...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/y92-052

    authors: Levy E

    更新日期:1992-04-01 00:00:00

  • Arterial remodeling: relation to hemodynamics.

    abstract::The structure of the artery wall is exquisitely sensitive to the hemodynamic forces imposed on it by blood pressure and blood flow. This sensitivity regulates growth and remodeling of arteries during development, and it regulates long-term adaptive restructuring of mature vessels. Since many vascular pathologies invol...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:

    authors: Langille BL

    更新日期:1996-07-01 00:00:00

  • Prevention of reperfusion-induced ventricular arrhythmias in isolated rat heart with magnesium.

    abstract::The effects of magnesium (from 1.2 to 7.2 mM) were investigated in isolated perfused rat heart subjected to coronary artery ligation and reperfusion. Increasing magnesium concentrations, of the medium containing 3.00 mM of calcium, induced a significant bradycardia and a protective effect towards reperfusion arrhythmi...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y90-105

    authors: Bril A,Rochette L

    更新日期:1990-06-01 00:00:00

  • Role of nitric oxide in mediating cardiovascular alterations accompanying heart failure in rats.

    abstract::The present study was designed to evaluate the role of endothelial NO in the hemodynamics and vascular changes that occur in heart failure following myocardial infarction in rats. Left ventricular systolic pressure (LVSP), mean blood pressure (MBP), aortic morphology (media thickness) and reactivity were evaluated in ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y04-038

    authors: da Cunha V,Stefanon I,Mill JG

    更新日期:2004-06-01 00:00:00

  • Epileptiform seizures in domestic fowl. V. The anticonvulsant activity of delta9-tetrahydrocannabinol.

    abstract::The anticonvulsant activity of delta9-tetrahydrocannabinol (delta9-THC) has been determined against seizures induced in epileptic chickens by intermittent photic stimulation (IPS) and in epileptic and nonepileptic chickens by Metrazol. Intravenous administration of the drug reduced both the severity and incidence of s...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y75-140

    authors: Johnson DD,McNeill JR,Crawford RD,Wilcox WC

    更新日期:1975-12-01 00:00:00

  • Short-term fluctuations in the concentration of cortisol and progesterone in fetal plasma, maternal plasma, and amniotic and allantoic fluids from sheep during late pregnancy.

    abstract::Fluctuations in the concentrations of cortisol and progesterone in fetal plasma, maternal plasma, and amniotic and allantoic fluids were measured in samples taken at 10-min intervals over a 90-min period from three groups of sheep sampled at different times during late pregnancy. During the last 30 days of gestation t...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y81-041

    authors: Challis JR,Patrick JE,Cross J,Workewych J,Manchester E,Power S

    更新日期:1981-03-01 00:00:00

  • Mode of action of ergonovine on isolated porcine coronary artery: the role of Ca2+.

    abstract::Coronary vasoconstrictor responses to ergonovine were examined in helical coronary arterial strips of young swine. Both ergonovine and serotonin (5-hydroxytryptamine) produced dose-dependent contractions of the strips. The distal region (less than 1.00 mm outer diameter) of the circumflex coronary artery was most sens...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y83-106

    authors: Rosenberger LB,Griffin MA,Stanton HC

    更新日期:1983-07-01 00:00:00

  • Fatty acid synthesis and generation of glycerol-3-phosphate in brown adipose tissue from rats fed a cafeteria diet.

    abstract::In vivo fatty acid synthesis and the pathways of glycerol-3-phosphate (G3P) production were investigated in brown adipose tissue (BAT) from rats fed a cafeteria diet for 3 weeks. In spite of BAT activation, the diet promoted an increase in the carcass fatty acid content. Plasma insulin levels were markedly increased i...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y08-052

    authors: Chaves VE,Frasson D,Martins-Santos ME,Navegantes LC,Galban VD,Garófalo MA,Kettelhut IC,Migliorini RH

    更新日期:2008-07-01 00:00:00

  • Dissociation constants (KA) and relative efficacies of sympathomimetic amines in isolated atria during hypothermia-induced supersensitivity.

    abstract::Hypothermia increases the sensitivity of isolated cardiac muscle to stimulation by beta-adrenoceptor agonists. The purpose of this study was to determine pharmacologically whether this supersensitivity is associated with a change in the affinity of agonists for the receptor. The positive inotropic and chronotropic res...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y83-088

    authors: Broadley KJ,McNeill JH

    更新日期:1983-06-01 00:00:00

  • Reciprocal regulation of cholesterol and beta amyloid at the subcellular level in Alzheimer's disease.

    abstract::Since the discovery that apolipoprotein E, a cholesterol transport protein, is a major risk factor for Alzheimer's disease (AD) development, there has been a remarkable interest in understanding the many facets of the relationship between cholesterol and AD. Several lines of evidence have demonstrated the importance o...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章,评审

    doi:10.1139/y2012-076

    authors: Posse de Chaves E

    更新日期:2012-06-01 00:00:00

  • Hydrogen peroxide induces heat shock protein and proto-oncogene mRNA accumulation in Xenopus laevis A6 kidney epithelial cells.

    abstract::In this study, we examined the effect of hydrogen peroxide on the accumulation of various mRNAs encoding heat shock proteins (hsps) and proto-oncogenes in Xenopus A6 kidney epithelial cells. Hydrogen peroxide treatment enhanced the accumulation of hsp90, hsp70, hsp30, c-jun, c-fos, and actin mRNAs with distinct tempor...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y04-059

    authors: Muller M,Gauley J,Heikkila JJ

    更新日期:2004-07-01 00:00:00

  • Different sites of membrane action for tetrodotoxin and lipid-soluble anesthetics.

    abstract::1. To examine whether different local anesthetics act on different or similar sites in the nerve membrane, the effects of combinations of lipid-soluble anesthetics and tetrodotoxin (TTX) were studied on the compound action potential of the frog sciatic nerve. 2. The nerve-blocking potency of TTX was potentiated 5-fold...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y75-073

    authors: Staiman AL,Seeman P

    更新日期:1975-08-01 00:00:00

  • Prior glucose deprivation increases the first phase of glucose-induced insulin response: possible involvement of endogenous ATP and (or) ADP.

    abstract::A possible implication of endogenously released ATP and (or) ADP in insulin response to glucose stimulation was investigated in the isolated rat pancreas. The first phase of insulin response to the same glucose concentration (8.3 mM) was much higher in pancreas previously perfused in the absence of glucose than in pan...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y93-086

    authors: Chapal J,Bertrand G,Hillaire-Buys D,Gross R,Loubatières-Mariani MM

    更新日期:1993-08-01 00:00:00

  • Posttetanic potentiation and skeletal muscle fatigue: interactions with caffeine.

    abstract::The purpose of this study was to determine the interaction of three factors that modify twitch contraction amplitude in the rat gastrocnemius muscle in situ: posttetanic potentiation, fatigue, and caffeine. Posttetanic (200 Hz for 1 s) twitch responses were observed before and after 15 Hz stimulation for 6 min (group ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y87-046

    authors: MacIntosh BR,Gardiner PF

    更新日期:1987-02-01 00:00:00

  • Inhibitory effects of tetramethyl and tetraethyl derivatives of pyrazine on dog saphenous vein.

    abstract::The effects of two structurally similar pyrazine derivatives, tetramethylpyrazine (TMP) and tetraethylpyrazine (TEP) on the contractile responses of dog saphenous vein to KCl (via membrane depolarization), phenylephrine (PHE, alpha 1-adrenergic agonist), and B-HT 920 (alpha 2-adrenergic agonist) were investigated. The...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y91-173

    authors: Wang ZL,Kwan CY,Ohta A,Chen MC

    更新日期:1991-08-01 00:00:00

  • Immediate hypersensitivity reactions in the guinea-pig conjunctiva: studies with a second-generation leukotriene D4 receptor antagonist, MK-571.

    abstract::The role of leukotriene D4 (LTD4) as a mediator of immediate hypersensitivity reactions in the guinea-pig conjunctiva was examined using a potent, second-generation LTD4 receptor antagonist, MK-571 (also known as L-660,711). The microvascular permeability changes in the guinea-pig conjunctiva following challenge with ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y89-132

    authors: Chan CC,Dagenais F,Firby P,Foster A,Ford-Hutchinson AW

    更新日期:1989-08-01 00:00:00

  • Differences in myocardial ischemic tolerance between 1- and 7-day-old rabbits.

    abstract::Between 1 and 7 days of life, the newborn rabbit heart shifts from predominantly using carbohydrates to predominantly using fatty acids as an energy substrate. We therefore used isolated working hearts from 1- or 7-day-old rabbits to determine the effects of fatty acids on myocardial glucose use and the ability of hea...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y92-184

    authors: Lopaschuk GD,Spafford MA

    更新日期:1992-10-01 00:00:00

  • ATP-MgCl2 restores renal microcirculation following trauma and severe hemorrhage.

    abstract::Although ATP-MgCl2 enhances the recovery of renal function after ischemia and reperfusion, it is not known whether this agent has any beneficial effects on renal microcirculation and function in a nonheparinized model of trauma and severe hemorrhage. To study this, a midline laparotomy was performed (i.e., trauma indu...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y92-044

    authors: Wang P,Zhou M,Rana MW,Singh G,Ba ZF,Ayala A,Chaudry IH

    更新日期:1992-03-01 00:00:00

  • Role of I(K) and I(f) in the pacemaker mechanisms of sino-atrial node myocytes.

    abstract::The role of I(K) (delayed rectifier current) and I(f) (hyperpolarization-activated current) in dominant and subsidiary pacemaker ranges was studied in single myocytes isolated from the guinea pig sino-atrial node by means of a perforated patch-clamp technique. In the dominant pacemaker range (approx. -55 to -40 mV), I...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:

    authors: Zhang H,Vassalle M

    更新日期:2001-12-01 00:00:00

  • The effect of ouabain on the guinea pig ileum longitudinal smooth muscle: 1. ATPase activities in a sarcolemma-enriched fraction prepared with the aid of divalent cation depletion of the intact muscle.

    abstract::Depletion of divalent cations before fractionation of the longitudinal muscle of the guinea pig ileum yielded a sarcolemma-enriched microsomal fraction free of mitochondria. A major portion of the ATPase activity in the presence of Mg, Na, and K was due to stimulation by Na alone. A further small stimulation by K was ...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y77-162

    authors: James MR,Roufogalis BD

    更新日期:1977-10-01 00:00:00

  • Forskolin and phosphodiesterase inhibitors release adenosine but inhibit morphine-evoked release of adenosine from spinal cord synaptosomes.

    abstract::The effects of forskolin, Ro 20-1724, rolipram, and 3-isobutyl-1-methylxanthine (IBMX) on morphine-evoked release of adenosine from dorsal spinal cord synaptosomes were evaluated to examine the potential involvement of cyclic AMP in this action of morphine. Ro 20-1724 (1-100 microM), rolipram (1-100 microM), and forsk...

    journal_title:Canadian journal of physiology and pharmacology

    pub_type: 杂志文章

    doi:10.1139/y91-133

    authors: Nicholson D,White TD,Sawynok J

    更新日期:1991-06-01 00:00:00