Applicability of upflow anaerobic sludge blanket and dynamic membrane-coupled process for the treatment of municipal wastewater.

abstract：:The Escherichia coli RNase G is known as an endoribonuclease responsible for the 5'-end maturation of 16S rRNA and degradation of several specific mRNAs such as adhE and eno mRNAs. In this study, we found that an RNase G mutant derived from the MC1061 strain did not grow on a glucose minimal medium. Genetic analysis r...

journal_title：Applied microbiology and biotechnology

authors： Sakai T,Nakamura N,Umitsuki G,Nagai K,Wachi M

更新日期：2007-08-01 00:00:00

abstract：:Carotenoids such as beta-carotene, lycopene, and antheraxanthin have plenty of scientific and commercial value. The comprehensive investigation of carotenoids drives people to improve and develop all kinds of analytical techniques to approach or even achieve "versatile" analysis. The metabolic engineering efforts in p...

journal_title：Applied microbiology and biotechnology

authors： Liu GN,Zhu YH,Jiang JG

更新日期：2009-07-01 00:00:00

abstract：:A recombinant form of the sweet-tasting protein thaumatin has been produced in the filamentous fungus Aspergillus niger var. awamori. Expression cassettes containing a synthetic gene encoding thaumatin II were prepared and used to transform Aspergillus niger var. awamori strain NRRL312. Several fungal strains capable ...

journal_title：Applied microbiology and biotechnology

authors： Faus I,del Moral C,Adroer N,del Río JL,Patiño C,Sisniega H,Casas C,Bladé J,Rubio V

更新日期：1998-04-01 00:00:00

abstract：:Phytases are phosphohydrolases that initiate the sequential hydrolysis of phosphate from phytate, which is the main storage form of phosphorous in numerous plant seeds, especially in cereals and grains. Phytate is indigestible for most monogastric animals, such as poultry, swine, fish, and humans; therefore, microbial...

journal_title：Applied microbiology and biotechnology

authors： Körfer G,Novoa C,Kern J,Balla E,Grütering C,Davari MD,Martinez R,Vojcic L,Schwaneberg U

更新日期：2018-11-01 00:00:00

abstract：:Oleaginous yeasts (18 strains) were grown in ethanol media under various cultivation conditions (33 biomass samples). It was found that lipid and lipid-free fractions of dry biomass have elemental composition and biomass reductivity very close to values which can be considered as biological constants. The energy conte...

journal_title：Applied microbiology and biotechnology

authors： Minkevich IG,Dedyukhina EG,Chistyakova TI

更新日期：2010-10-01 00:00:00

abstract：:A reactive iron barrier in a contaminated aquifer with low pH was found to dechlorinate 1,2-dichloroethane (1,2-DCA) in situ. This chlorinated ethane is known to resist abiotic reduction by zero valent iron. Samples taken up-gradient and within the barrier were used to inoculate anaerobic batch cultures amended with v...

journal_title：Applied microbiology and biotechnology

authors： Zemb O,Lee M,Low A,Manefield M

更新日期：2010-09-01 00:00:00

abstract：:Fatty acid desaturases play vital roles in the synthesis of unsaturated fatty acids. In this study, Δ12 and Δ12/Δ15 fatty acid desaturases of the oleaginous yeast Lipomyces starkeyi, termed LsFad2 and LsFad3, respectively, were identified and characterized. Saccharomyces cerevisiae expressing LsFAD2 converted oleic ac...

journal_title：Applied microbiology and biotechnology

authors： Matsuzawa T,Maehara T,Kamisaka Y,Ara S,Takaku H,Yaoi K

更新日期：2018-10-01 00:00:00

abstract：:Understanding the effect of conserved domains reconstruction of alginate lyases on action mode is essential for their application and in-depth study. We report the expression and action mode of recombinant alginate lyase (AlyM) and its conserved domain reconstruction forms (AlyMΔCBM, cAlyM, and AlyMΔ58C). The enzymati...

journal_title：Applied microbiology and biotechnology

authors： Yang M,Li N,Yang S,Yu Y,Han Z,Li L,Mou H

更新日期：2019-01-01 00:00:00

abstract：:To investigate how the microbial community in activated sludge responded to high antibiotic levels, a bench-scale aerobic wastewater treatment system was used to treat oxytetracycline (OTC) mother liquor (OTC-ML). Removal efficiency of chemical oxygen demand decreased from 64.9 to 51.0 % when the OTC level increased f...

journal_title：Applied microbiology and biotechnology

authors： Liu M,Zhang Y,Ding R,Gao Y,Yang M

更新日期：2013-10-01 00:00:00

abstract：:River-bay system is a transitional zone connecting land and ocean and an important natural source for methane emission. Methanogens play important roles in the global greenhouse gas budget and carbon cycle since they produce methane. The abundance and community assemblage of methanogens in such a dynamic system are no...

journal_title：Applied microbiology and biotechnology

authors： Zhang CJ,Chen YL,Pan J,Wang YM,Li M

更新日期：2020-05-01 00:00:00

abstract：:Smokeless tobacco (ST) products are used worldwide and are a major public health concern. In addition to harmful chemicals found in these products, microbes found in ST products are believed to be responsible for generating harmful tobacco-specific nitrosamines (TSNAs), the most abundant carcinogens in ST. These micro...

journal_title：Applied microbiology and biotechnology

authors： Tyx RE,Rivera AJ,Keong LM,Stanfill SB

更新日期：2020-01-01 00:00:00

abstract：:We report an immuno-magnetic separation system developed by the immobilization of pathogen-specific antibodies on the core-shell magnetic beads. The magnetic beads were grafted with glycidylmethacrylate (GMA) using surface-initiated atom transfer radical polymerization (SI-ATRP). For immuno-magnetic separation (IMS) o...

journal_title：Applied microbiology and biotechnology

authors： Ozalp VC,Bayramoglu G,Arica MY,Oktem HA

更新日期：2013-11-01 00:00:00

abstract：:Agar is a major cell wall carbohydrate of red macroalgae (Rhodophyta). Sugars derived from agar, such as agarooligosaccharides (AOSs), neoagarooligosaccharides (NAOSs), neoagarobiose (NAB), and 3,6-anhydro-L-galactose (L-AHG), possess various physiological activities. These agar-derived sugars can be produced by hydro...

journal_title：Applied microbiology and biotechnology

authors： Yun EJ,Yu S,Kim KH

更新日期：2017-07-01 00:00:00

abstract：:Nitrite-dependent anaerobic methane oxidation (N-DAMO), which couples anaerobic methane oxidation and nitrite reduction, is a recently discovered bioprocess coupling microbial nitrogen and carbon cycles. The discovery of this microbial process challenges the traditional knowledge of global methane sinks and nitrogen l...

journal_title：Applied microbiology and biotechnology

authors： Wang J,Shen L,He Z,Hu J,Cai Z,Zheng P,Hu B

更新日期：2017-11-01 00:00:00

abstract：:A key factor contributing to the variability in the microbial kinetic parameters reported from batch assays is parameter identifiability, i.e., the ability of the mathematical routine used for parameter estimation to provide unique estimates of the individual parameter values. This work encompassed a three-part evalua...

journal_title：Applied microbiology and biotechnology

authors： Seagren EA,Kim H,Smets BF

更新日期：2003-05-01 00:00:00

abstract：:The behaviour of dry baker's yeast ( Saccharomyces cerevisiae type II, Sigma) used as biocatalyst without preliminary growth for the synthesis of 2-heptanol from 2-heptanone in a biphasic system is presented. Cells undergo intracellular trehalose consumption with a stoichiometric ethanol production during the first 15...

journal_title：Applied microbiology and biotechnology

authors： Cappaert L,Larroche C

更新日期：2004-06-01 00:00:00

abstract：:Cupriavidus necator JMP134 utilizes meta-nitrophenol (MNP) as a sole source of carbon, nitrogen, and energy. The metabolic reconstruction of MNP degradation performed in silico suggested that the mnp cluster might have played important roles in MNP degradation. In order to experimentally confirm the prediction, we hav...

journal_title：Applied microbiology and biotechnology

authors： Yin Y,Xiao Y,Liu HZ,Hao F,Rayner S,Tang H,Zhou NY

更新日期：2010-08-01 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is widely used for fermentative production of amino acids. The world production of L-lysine has surpassed 2 million tons per year. Glucose uptake and phosphorylation by C. glutamicum mainly occur by the phosphotransferase system (PTS) and to lesser extent by inositol permea...

journal_title：Applied microbiology and biotechnology

authors： Pérez-García F,Peters-Wendisch P,Wendisch VF

更新日期：2016-09-01 00:00:00

abstract：:κ-Carrageenases exhibit apparent distinctions in gene sequence, molecular weight, enzyme properties, and posttranslational processes. In this study, a new κ-carrageenase gene named cgkZ was cloned from the marine bacterium Zobellia sp. ZM-2. The gene comprised an open reading frame of 1,638 bp and encoded 545 amino ac...

journal_title：Applied microbiology and biotechnology

authors： Liu Z,Li G,Mo Z,Mou H

更新日期：2013-12-01 00:00:00

abstract：:Glucose 6-phosphate dehydrogenase (G6PDH) is one of the most important dehydrogenases responsible for generating reduced NADPH for anabolism and is also the rate-limiting enzyme in the Entner-Doudoroff pathway. For in vitro biocatalysis, G6PDH must possess both high activity and good thermostability due to requirement...

journal_title：Applied microbiology and biotechnology

authors： Huang R,Chen H,Zhou W,Ma C,Zhang YP

更新日期：2018-04-01 00:00:00

abstract：:C-phycocyanin (C-PC) was extracted from fresh Spirulina platensis by deploying a species of non-pathogenic nitrogen-fixing bacteria, namely, Klebsiella pneumoniae. The algal slurry was neither washed nor centrifuged; the bacterial culture was poured into the slurry, the vessel sealed, and crude C-PC extracted after ab...

journal_title：Applied microbiology and biotechnology

authors： Zhu Y,Chen XB,Wang KB,Li YX,Bai KZ,Kuang TY,Ji HB

更新日期：2007-02-01 00:00:00

abstract：:Guanosine 5'-triphosphate (GTP) is the key substrate for biosynthesis of guanosine 5'-diphosphate (GDP)-L-fucose. In this study, improvement of GDP-L-fucose production was attempted by manipulating the biosynthetic pathway for guanosine nucleotides in recombinant Escherichia coli-producing GDP-L-fucose. The effects of...

journal_title：Applied microbiology and biotechnology

authors： Lee WH,Shin SY,Kim MD,Han NS,Seo JH

更新日期：2012-03-01 00:00:00

abstract：:The long-term usage of antibiotics has resulted in the evolution of multidrug-resistant bacteria. Unlike antibiotics, anti-virulence approaches target bacterial virulence without affecting cell viability, which may be less prone to develop drug resistance. Staphylococcus aureus is a major human pathogen that produces ...

journal_title：Applied microbiology and biotechnology

authors： Lee K,Lee JH,Kim SI,Cho MH,Lee J

更新日期：2014-11-01 00:00:00

abstract：:Biosurfactant rhamnolipids have been claimed to show biological activities of inhibiting the proliferation of cancer cells. In this study, the cytotoxicity of rhamnolipids was examined on four cancer cells (HepG2, Caco-2, Hela, MCF-7 cells) and two normal cells (HK-2 cell, primary hepatocyte). Interestingly, both canc...

journal_title：Applied microbiology and biotechnology

authors： Jiang L,Shen C,Long X,Zhang G,Meng Q

更新日期：2014-12-01 00:00:00

abstract：:Biosynthesis of Ds(+)-threo-isocitric acid from ethanol in the Yarrowia lipolytica batch and repeated-batch cultures was studied. Repeated-batch cultivation was found to provide for a good biosynthetic efficiency of the producer for as long as 748 h, probably due to maintenance of high activities of enzymes involved i...

journal_title：Applied microbiology and biotechnology

authors： Morgunov IG,Kamzolova SV,Karpukhina OV,Bokieva SB,Inozemtsev AN

更新日期：2019-04-01 00:00:00

abstract：:In recent years, a number of studies have been done on the physiological effects of water-soluble polysaccharides (WSPS) and their antitumor and immuno-enhancing properties. Many edible mushrooms, in particular those rich in WSPS, not only taste good but also contain ingredients beneficial to the physiology of the hum...

journal_title：Applied microbiology and biotechnology

authors： Wang JC,Hu SH,Liang ZC,Yeh CJ

更新日期：2005-06-01 00:00:00

abstract：:The TonB system functions in iron transport and has been identified in certain Gram-negative bacteria. Recently, we reported three TonB systems in the Aeromonas hydrophila Chinese epidemic strain NJ-35, but the functions of these systems have not been thoroughly elucidated to date. In this study, we investigated the r...

journal_title：Applied microbiology and biotechnology

authors： Dong Y,Geng J,Liu J,Pang M,Awan F,Lu C,Liu Y

更新日期：2019-05-01 00:00:00

abstract：:Nonribosomal peptides (NRPs), a large family of natural products, possess numerous pharmaceutically significant bioactivities. However, many native microbial producers of NRPs are not cultivable or have low production yields making mass production infeasible. The recombinant production of natural products in a surroga...

journal_title：Applied microbiology and biotechnology

authors： Li J,Jaitzig J,Hillig F,Süssmuth R,Neubauer P

更新日期：2014-01-01 00:00:00

abstract：:In this study, we employed TiO₂ enrichment and high accuracy liquid chromatography-mass spectrometry-mass spectrometry to identify the phosphoproteome of Clostridium acetobutyicum ATCC824 in acidogenesis and solventogenesis. As many as 82 phosphopeptides in 61 proteins, with 107 phosphorylated sites on serine, threoni...

journal_title：Applied microbiology and biotechnology

authors： Bai X,Ji Z

更新日期：2012-07-01 00:00:00

abstract：:Respiration measurement in shake flasks is introduced as a new method to characterize the metabolic activity of microorganisms during and after stress exposure. The major advantage of the new method is the possibility to determine the metabolic activity independent of manual sampling without the necessity to change th...

journal_title：Applied microbiology and biotechnology

authors： Seletzky JM,Noack U,Fricke J,Hahn S,Büchs J

更新日期：2006-10-01 00:00:00