Microfluidic perfusion bioreactor for optimization of microalgal lipid productivity.

Abstract:

:Nutrient deprivation in a batch process induces microbes to produce secondary metabolites while drastically constraining cellular growth. A microfluidic continuous perfusion system was designed and tested to culture microalgae, Chlamydomonas reinhardtii, under constant nutrient concentration slightly lower than normal condition. When cultured in 7.5%/7.5% of NH4+/PO42-, C. reinhardtii showed a 2.4-fold increase in TAG production with a 3.5-fold increase in biomass compared to level obtained under an only NH4+ depleted condition. The microfluidic continuous perfusion bioreactor with steady continuous nutrient flow can be used to optimize conditions for enhancing secondary metabolite production and increasing microbial biomass.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Paik SM,Sim SJ,Jeon NL

doi

10.1016/j.biortech.2017.02.050

subject

Has Abstract

pub_date

2017-06-01 00:00:00

pages

433-437

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(17)30173-6

journal_volume

233

pub_type

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