Abstract：
:The effects of surface properties of Saccharomyces cerevisiae strains 468/pGAC9 and 468 on adhesion to polyethyleneimine (PEI) and/glutaraldehyde (GA) pre-treated cotton (CT), polyester (PE), polyester+cotton (PECT), nylon (NL), polyurethane foam (PUF), and cellulose re-enforced polyurethane (CPU) fibers were investigated. Process parameters (circulation velocity, pH, ionic strength, media composition and surfactants) were also examined. 80%, 90%, and 35% of the cells were adsorbed onto unmodified CT, PUF, and PE, respectively. PEI-GA pre-treated CT and alkali treated PE yielded 25% and 60% cell adhesion, respectively. Adsorption rate (K(a)) ranged from 0.06 to 0.17 for CT and 0.06-0.16 for PE at varied pH. Adhesion increased by 15% in the presence of ethanol, low pH and ionic strength, and decreased by 23% in the presence of yeast extract and glucose. Shear flow and 1% Triton X-100 detached 62% and 36% nonviable cells from PE and CT, respectively, suggesting that cell immobilization in fibrous-bed bioreactors can be controlled to optimize cell density for long-term stability.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kilonzo P,Margaritis A,Bergougnou Mdoi
10.1016/j.biortech.2010.11.055subject
Has Abstractpub_date
2011-02-01 00:00:00pages
3662-72issue
4eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01858-4journal_volume
102pub_type
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