A second generation global analysis program for the recovery of complex inhomogeneous fluorescence decay kinetics.


:A fluorescence spectroscopy global data analysis environment is described. Within this analysis environment, multidimensional fluorescence decay data (time and frequency domains) can be analyzed in terms of a wide variety of photophysical models. A generalized compartmental analysis structure is utilized, where one can specify the functions used to link the various compartments together. All fitting parameters may be characterized by either discrete or distributed values. Applications of these new analysis programs to the examination of phase transitions in lipid/membrane systems are described.


Chem Phys Lipids


Beechem JM




Has Abstract


1989-06-01 00:00:00














  • Tailoring lipid nanoconstructs for the oral delivery of paliperidone: Formulation, optimization and in vitro evaluation.

    abstract:PURPOSE:The present research work involves Quality by Design (QbD)-based fabrication of lipid nanoconstructs (LNC) of paliperidone (PPD) bearing superior biopharmaceutical attributes. METHODS:LNC of paliperidone was prepared by melt emulsification-probe sonication and high-pressure homogenization method followed by op...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Rehman S,Nabi B,Baboota S,Ali J

    更新日期:2021-01-01 00:00:00

  • The phase transition behavior of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) model membrane influenced by 2,4-dichlorophenol--an FT-Raman spectroscopy study.

    abstract::The effect of 2,4-dichlorophenol (DCP) on the structures and phase transitions of fully hydrated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes was studied using FT-Raman spectroscopy. Whereas the Raman frequency shifts of the most frequently investigated bands of C-C and C-H stretching regions only indi...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Csiszár A,Koglin E,Meier RJ,Klumpp E

    更新日期:2006-02-01 00:00:00

  • Vitamin E - phosphatidylethanolamine interactions in mixed membranes with sphingomyelin: Studies by 2H NMR.

    abstract::Among the structurally diverse collection of lipids that comprise the membrane lipidome, polyunsaturated phospholipids are particularly vulnerable to oxidation. The role of α-tocopherol (vitamin E) is to protect this influential class of membrane phospholipid from oxidative damage. Whether lipid-lipid interactions pla...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Cavazos AT,Kinnun JJ,Williams JA,Wassall SR

    更新日期:2020-09-01 00:00:00

  • Calcium-induced aggregation of phosphatidylcholine vesicles containing free oleic acid.

    abstract::Small unilamellar vesicles (SUV) formed by egg yolk phosphatidylcholine (PC) and free oleic acid (OA) undergo aggregation induced by Ca2+ at pH greater than 7.0. The rate of the process, as monitored by turbidity changes, presents a linear dependence on phospholipid concentration and a hyperbolic dependence on Ca2+ co...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Ortiz A,Gomez-Fernandez JC

    更新日期:1988-04-01 00:00:00

  • Effect of membrane phase transition on long-time calcium-induced fusion of phosphatidylserine vesicles.

    abstract::Dynamic light scattering has been used to study the temperature dependence of the extent of long-time calcium-induced fusion of sonicated vesicles composed of various natural and synthetic phosphatidylserine with different acyl chains. The vesicles of each composition are found to exhibit a peak temperature in the vic...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Leung SO,Ho JT

    更新日期:1991-01-01 00:00:00

  • Infrared spectroscopy of glycolipids.

    abstract::The application of infrared spectroscopy to the physicochemical characterization of glycolipids as biologically important membrane constituents is described. In this contribution, the analysis of diacyl- and sphingosine-based (cerebroside, ganglioside) oligosaccharides and the class of lipid A-anchored lipooligo- and ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Brandenburg K,Seydel U

    更新日期:1998-11-01 00:00:00

  • Monolayer study of mixtures of diacetylenic phosphatidylcholine and phospholipids containing metal-chelating iminodiacetic acid headgroup.

    abstract::The mixing behavior of polymerizable diacetylenic phosphatidylcholine (1) with two diacetylenic phospholipids containing the iminodiacetic acid (IDA) functionality in the headgroup region was studied at the air-water interface. All three phospholipids contained tricosa-10,12-diynoyl acyl chains. In phospholipid 2, the...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Markowitz MA,Puranik DB,Singh A

    更新日期:1995-05-22 00:00:00

  • Combined force spectroscopy, AFM and calorimetric studies to reveal the nanostructural organization of biomimetic membranes.

    abstract::In this work we studied a binary lipid matrix of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG), a composition that mimics the inner membrane of Escherichia coli. More specifically, liposomes with varying fractions of POPG were analys...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Suárez-Germà C,Morros A,Montero MT,Hernández-Borrell J,Domènech Ò

    更新日期:2014-10-01 00:00:00

  • Effect of lipid environment on amyloid fibril formation of human serum amyloid A.

    abstract::Human serum amyloid A (SAA) is a precursor protein of AA amyloidosis and a component of high-density lipoproteins (HDLs), thus it is essential to investigate the amyloid fibril formation of SAA under a lipid environment. We used synthetic fragment peptides corresponding to the N-terminal (residues 1-27) and central (r...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Tanaka M,Nishimura A,Takeshita H,Takase H,Yamada T,Mukai T

    更新日期:2017-01-01 00:00:00

  • Activation of lipoprotein lipase by apolipoprotein C-II is modulated by the COOH terminal region of apolipoprotein C-III.

    abstract::The in vitro effect of apolipoprotein C-II (apo C-II) on the apolipoprotein C-III (apo C-III) induced activation of bovine milk lipoprotein lipase (LPL) was studied in vitro using a synthetic substrate. Apo C-III effectively inhibited, in a dose-dependent manner, the activation of lipoprotein lipase induced by apo C-I...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Catapano AL

    更新日期:1987-10-01 00:00:00

  • Diffusion of hydrophilic probes in bicontinuous lipidic cubic phase.

    abstract::The lipidic cubic phase was prepared by mixing monoolein (monooleoyl-rac-glycerol, MO) with water in 64:36% ratio and applied to the solid support-glassy carbon or platinum electrodes. Highly viscous, homogeneous and transparent cubic phase film remained stable and firmly attached to the electrode surface. In order to...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Rowiński P,Korytkowska A,Bilewicz R

    更新日期:2003-07-01 00:00:00

  • The effect of aggregation state of amphotericin-B on its interactions with cholesterol- or ergosterol-containing phosphatidylcholine monolayers.

    abstract::Amphotericin B (AmB) is the most effective antibiotic used in the treatment of systemic fungal infections. It is generally thought that the activity of this drug results from its interaction with ergosterol, the main sterol of fungi membranes. However, AmB also interacts with cholesterol, the major sterol of mammal me...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Barwicz J,Tancrède P

    更新日期:1997-02-28 00:00:00

  • A particle concentration fluorescence immunoassay for Lp(a).

    abstract::The quantitation of Lp(a) by immunoassay presents a major technical problem, because the molecular mass of the (a) protein of Lp(a) can vary between 419,000 and 838,000 Da (Gaubatz et al. (1990) J. Lipid Res. 31, 603-612), and this variability is determined by at least 24 alleles of the (a) gene. In an attempt to over...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Kottke BA,Bren ND

    更新日期:1994-01-01 00:00:00

  • Involvement of sphingomyelinases in TNF signaling pathways.

    abstract::Sphingomyelin (N-acylsphingosin-1-phosphorylcholine) is a phospholipid preferentially found in the plasma membrane of mammalian cells. Signaling through the sphingomyelin pathway is associated with generation of ceramide, which acts as a second messenger in activating a variety of cellular functions. Ceramide belongs ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Krönke M

    更新日期:1999-11-01 00:00:00

  • NMR-based molecular ruler for determining the depth of intercalants within the lipid bilayer: Part III: studies on keto esters and acids.

    abstract::The development of "molecular rulers" would allow one to quantitatively locate the penetration depth of intercalants within lipid bilayers. To this end, an attempt was made to correlate the (13)C NMR chemical shift of polarizable "reporter" carbons (e.g., carbonyls) of intercalants within DMPC liposomal bilayers - wit...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Afri M,Alexenberg C,Aped P,Bodner E,Cohen S,Ejgenburg M,Eliyahu S,Gilinsky-Sharon P,Harel Y,Naqqash ME,Porat H,Ranz A,Frimer AA

    更新日期:2014-12-01 00:00:00

  • Production of conjugated linoleic acids through KOH-catalyzed dehydration of ricinoleic acid.

    abstract::Production of conjugated linoleic acids (CLA) using castor oil as starting material involves conversion of ricinoleic acid to methyl 12-mesyloxy-octadec-9-enoate (MMOE) followed by dehydration. This process usually uses 1,8-diazabicyclo-(5.4.0)-undec-7-ene (DBU) as an expensive dehydrating reagent. The present study r...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Yang L,Huang Y,Wang HQ,Chen ZY

    更新日期:2002-10-01 00:00:00

  • Reprint of: "Synthetic lipids and their role in defining macromolecular assemblies".

    abstract::Lipids have a variety of physiological roles, ranging from structural and biophysical contributions to membrane functions to signaling contributions in normal and abnormal physiology. This review highlights some of the contributions made by Robert Bittman to our understanding of lipid assemblies through the production...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Parrill AL

    更新日期:2016-01-01 00:00:00

  • Characterization of liposomes and other lipid assemblies by multiprobe fluorescence polarization.

    abstract::This minireview describes the use of fluorescence polarization to characterize the dynamics and organization of biological membranes, lipoproteins, liposomes and other lipid-based assemblies. The advantages, disadvantages and limitations of this method are discussed. There is special emphasis on the multiprobe approac...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Borenstain V,Barenholz Y

    更新日期:1993-09-01 00:00:00

  • Genetic control of glycolipid expression.

    abstract::A polymorphic variation of sialic acid species of sialosyllactosylceramide was found in dog erythrocytes. The analysis of the glycolipids in the erythrocytes of the individual dogs in a family of a Japanese breed of dog, Shiba-Inu, showed that the expression of sialosyllactosylceramide containing N-glycolylneuraminic ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Yamakawa T,Suzuki A,Hashimoto Y

    更新日期:1986-12-15 00:00:00

  • Carbohydrate liquid crystals: synthesis and characterisation of the methyl-6-O-(n-acyl)-α-D-glucopyranosides.

    abstract::Seven members of the methyl-6-O-(n-acyl)-α-D-glucopyranosides have been synthesised and their transitional properties determined. The undecanoyl and octadecanoyl members do not exhibit liquid crystallinity while the members having chain lengths between dodecanoyl and hexadecanoyl exhibit a monotropic smectic A phase. ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Cook AG,Wardell JL,Imrie CT

    更新日期:2011-02-01 00:00:00

  • Bicontinuous cubic phase: a model for investigating the effects on a lipid bilayer due to a foreign substance.

    abstract::Biological effects of foreign substances are usually caused by their interaction within lipid bilayers with the membrane lipids. It is therefore desirable in many situations to determine the effects and the partition of xenobiotic substances, as well as drugs, added to amphiphile-water systems. Bicontinuous cubic liqu...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Engblom J

    更新日期:1996-12-20 00:00:00

  • Mitochondrial alterations in apoptosis.

    abstract::Besides their conventional role as energy suppliers for the cell, mitochondria in vertebrates are active regulators of apoptosis. They release apoptotic factors from the intermembrane space into the cytosol through a mechanism that involves the Bcl-2 protein family, mediating permeabilization of the outer mitochondria...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Cosentino K,García-Sáez AJ

    更新日期:2014-07-01 00:00:00

  • Cholesterol-induced suppression of membrane elastic fluctuations at the atomistic level.

    abstract::Applications of solid-state NMR spectroscopy for investigating the influences of lipid-cholesterol interactions on membrane fluctuations are reviewed in this paper. Emphasis is placed on understanding the energy landscapes and fluctuations at an emergent atomistic level. Solid-state (2)H NMR spectroscopy directly meas...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Molugu TR,Brown MF

    更新日期:2016-09-01 00:00:00

  • Phase state and surface topography of palmitoyl-ceramide monolayers.

    abstract::In cell biology (and in many biophysical) studies there is a natural tendency to consider ceramide as a highly condensed, solid-type lipid conferring rigidity and close packing to biomembranes. In the present work we advanced the understanding of the phase behavior of palmitoyl-ceramide restricted to a planar interfac...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Fanani ML,Maggio B

    更新日期:2010-06-01 00:00:00

  • Diffusion of hydrogen peroxide across DPPC large unilamellar liposomes.

    abstract::The decomposition of hydrogen peroxide catalyzed by catalase entrapped in the pool of dipalmitoylphosphatidyl choline unilamellar liposomes has been studied. The rate of the process was evaluated by following the production of oxygen as a function of time. Under the experimental conditions employed the rate of oxygen ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Abuin E,Lissi E,Ahumada M

    更新日期:2012-09-01 00:00:00

  • Lipolytic activity of porcine pancreas lipase on fatty acid esters of dialkylglycerols: a structural basis for the design of new substrates for the assay of pancreatic lipases activity.

    abstract::For the design of new synthetic substrates for the assay of pancreatic lipases activity, acyl dialkylglycerols of variable chain length were prepared. Titrimetric assay of these substrates showed the highest lipolytic activity of porcine pancreas lipase (pPL) with butanoyl dibutylglycerol. The activity is lower but co...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Ciuffreda P,Loseto A,Manzocchi A,Santaniello E

    更新日期:2001-06-01 00:00:00

  • Lipid microdomains in dimyristoylphosphatidylcholine-ceramide liposomes.

    abstract::Binary membranes composed of dimyristoylphosphatidylcholine (DMPC) and natural ceramide (up to a mole fraction Xcer = 0.25) were investigated by measuring the excimer:monomer fluorescence emission intensity ratio IE:IM for the pyrene labeled phospholipid probe 1-palmitoyl-2[(pyren-1-yl)]decanoyl-sn-glycero-3-phosphoch...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Holopainen JM,Lehtonen JY,Kinnunen PK

    更新日期:1997-08-08 00:00:00

  • The role of the ganglioside lipid moiety in the process of ganglioside-cell interactions.

    abstract::The role of the ceramide moiety of gangliosides, together with the deriving aggregative properties of ganglioside in solution, in the process of ganglioside-cell interactions was studied. The natural GM1(stearoyl) and the synthetic GM1(acetyl), containing the stearoyl and acetyl groups as the acyl moiety, respectively...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Bassi R,Sonnino S

    更新日期:1992-07-01 00:00:00

  • NMR and calorimetric studies of changes in phase transition of head group modified phospholipids.

    abstract::The 13C- and 31P-NMR spectra and DSC thermograms of unsonicated dispersions consisting of phospholipids of varying methylation stages of the propanolamine head group were investigated. Methylation of the head group was accompanied by a corresponding reduction in the phase transition temperatures. In the liquid crystal...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Gawrisch K,Arnold K,Rüger HJ,Kertscher P,Nuhn P

    更新日期:1977-12-01 00:00:00

  • Raman spectroscopy investigation of various saturated monoacid triglycerides.

    abstract::A study of the vibrational behavior of five saturated monoacid triacylglycerides is performed by Raman spectroscopy at room temperature in the 3100-500 cm(-1) spectral range. The splitting of the CO stretching mode leads to conclude on the existence of two or three geometries of CO in the "knot" group OCO. The CO stre...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Bresson S,El Marssi M,Khelifa B

    更新日期:2005-04-01 00:00:00