Abstract:
:Thio analogs of platelet activating factor (PAF) are of great interest because they exhibit antineoplastic properties both in vitro and in vivo. In contrast to most known anticancer agents, these lipids appear not to act through the synthesis and function of DNA and, therefore, offer a new avenue of approaching cancer chemotherapy. We have examined the conformational properties of 1-thiohexadecyl-2-O-methyl-S-glycero-3-phosphocholine (ET-S-16-OCH3) in organic solvents and in micelles. The conformational analysis was based on a combination of 1D, 2D NMR spectroscopy and molecular graphics. 1H and 13C spin lattice relaxation time (T1) experiments were also performed to study the dynamic properties of this molecule. The picture emerging from these studies is as follows. The alkyl chain of ET-S-16-OCH3 is the most mobile part of the molecule both in CDCl3/CD3OD and in micelles and exists as a mixture of interconverting conformers including an extended all trans and several low energy conformers with one or more gauche segments. This creates a twisting of the chain and facilitates a spatial communication between the alkyl chain and the glycerol backbone as well as between the alkyl chain and the headgroup. The methylene groups of the thioglycerol backbone and the headgroup are the least mobile while the methine group of the thioglycerol backbone appears to have an intermediate mobility. The conformation of the thioether lipid in the two media may be of relevance during its interaction with its site of action, the cellular membrane. Such a conformation may also play an important role in determining the selectivity of this interaction with different cell membranes.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Mavromoustakos T,Theodoropoulou E,Yang DP,Lin SY,Koufaki M,Makriyannis Adoi
10.1016/s0009-3084(96)02615-1subject
Has Abstractpub_date
1996-11-01 00:00:00pages
21-34issue
1eissn
0009-3084issn
1873-2941pii
S0009308496026151journal_volume
84pub_type
杂志文章abstract::Low density lipoprotein (LDL) cholesterol is known to be oxidized both in vitro and in vivo giving rise to oxygenated sterols. Conflicting results, however, have been reported concerning both the nature and the relative concentrations of these compounds in oxidized human LDL. We examined the extracts obtained from Cu(...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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journal_title:Chemistry and physics of lipids
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更新日期:2008-06-01 00:00:00
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
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doi:10.1016/0009-3084(90)90119-c
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/j.chemphyslip.2018.09.013
更新日期:2018-11-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(96)02535-2
更新日期:1996-06-17 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(78)90030-0
更新日期:1978-10-01 00:00:00
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journal_title:Chemistry and physics of lipids
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doi:10.1016/j.chemphyslip.2010.04.008
更新日期:2010-06-01 00:00:00
abstract::New 1,20-substituted eicosanes carrying phosphate headgroups and readily derivatizable thiol, maleimido, and activated carboxylic ester moieties were prepared. The C20-backbone of these molecules was assembled by a halopolycarbon homologation from 1,8-dichlorooctane and 1,6-dibromohexane. 20-Mercapto- and 20-maleimido...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2004.09.016
更新日期:2005-01-01 00:00:00
abstract::A binding protein is described for certain oxygenated derivatives of cholesterol which suppress 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol synthesis in cultured mammalian cells. This protein is found in the cytosolic fraction of many cell types and is distinct from cytosolic proteins which bind ch...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(85)90066-0
更新日期:1985-08-30 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(88)90115-6
更新日期:1988-01-01 00:00:00
abstract::Age-related fluorescent and cross-linked proteins increase with lipid oxidation of tissues. The fluorophores and cross-links have been considered to be conjugated Schiff bases between amino groups of proteins and malonaldehyde. Our recent studies showed that the fluorophores produced in the in vitro reaction of protei...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/0009-3084(87)90054-5
更新日期:1987-07-01 00:00:00
abstract::Lipids play an important role in the maturation, viability and function of sperm cells. In this study, we examined the neutral and polar lipid composition of boar spermatozoa by thin-layer chromatography/mass spectrometry. Main representatives of the neutral lipid classes were diacylglycerols containing saturated (myr...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2013.11.001
更新日期:2014-01-01 00:00:00
abstract::Monolactone (NeuAc1-9NeuAc linkage) formation of a ganglioside, GD1b, was observed at 20 degrees C on solutions ranging in concentration from 0.008 to 1.7 mM and in pH from 2 to 8.1, by following the change of ellipicity at 235 nm, where lactonized gangliosides showed a strong Cotton effect. The lactonization was foun...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(94)90051-5
更新日期:1994-03-31 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/s0009-3084(00)00123-7
更新日期:2000-04-01 00:00:00
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journal_title:Chemistry and physics of lipids
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更新日期:1997-08-08 00:00:00
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更新日期:2014-02-01 00:00:00
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journal_title:Chemistry and physics of lipids
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更新日期:2002-12-01 00:00:00
abstract::The work reported herein deals with the synthesis and the aggregation behavior studies of synthetic lipids bearing a non-ionic polar head made up of a tris(hydroxymethyl) aminomethane (tris) moiety linked with an aminoglycerol interface. The hydrophobic chains with variable lengths were grafted onto the hydroxyl funct...
journal_title:Chemistry and physics of lipids
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doi:10.1016/j.chemphyslip.2005.03.006
更新日期:2005-07-01 00:00:00
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journal_title:Chemistry and physics of lipids
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doi:10.1016/j.chemphyslip.2013.12.010
更新日期:2014-02-01 00:00:00
abstract::A new polymerizable surfactant, 12-methacryloyl dodecylphosphocholine (MDPC), has been synthesized using a three-step procedure in moderate yield. Phase transitions were characterized by DSC and phase behavior in water was determined by surface tension and polarizing microscopy. MDPC showed typical surfactant behavior...
journal_title:Chemistry and physics of lipids
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doi:10.1016/j.chemphyslip.2010.03.001
更新日期:2010-05-01 00:00:00
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journal_title:Chemistry and physics of lipids
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doi:10.1016/j.chemphyslip.2017.12.004
更新日期:2018-05-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(86)90116-7
更新日期:1986-03-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2005.02.015
更新日期:2005-06-01 00:00:00
abstract::Oxidation of low density lipoprotein (LDL) has been proposed to be involved in the pathogenesis of atherosclerosis. We have previously shown that LDL can be oxidised by iron in lysosomes. As the iron-storage protein ferritin might enter lysosomes by autophagy, we have investigated the ability of ferritin to catalyse L...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2018.09.016
更新日期:2018-12-01 00:00:00
abstract::The activity of phytosterols on human organism includes the ability of these compounds to incorporate into membranes. In the consequence the plant sterols are able to increase total sterol concentration in membrane or/and to replace cholesterol molecules. The aim of this work was to compare the influence of both these...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2010.07.001
更新日期:2010-09-01 00:00:00
abstract::Enveloped viruses, which include many medically important viruses such as human immunodeficiency virus, influenza virus and hepatitis C virus, are intracellular parasites that acquire lipid envelopes from their host cells. Success of replication is intimately linked to their ability to hijack host cell mechanisms, par...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/j.chemphyslip.2010.03.002
更新日期:2010-06-01 00:00:00