Abstract:
:A binding protein is described for certain oxygenated derivatives of cholesterol which suppress 3-hydroxy-3-methylglutaryl coenzyme A reductase and cholesterol synthesis in cultured mammalian cells. This protein is found in the cytosolic fraction of many cell types and is distinct from cytosolic proteins which bind cholesterol. The relative binding affinity of a wide variety of oxysterols correlates with their ability to suppress reductase and it is proposed that the binding protein functions as a receptor for endogenous regulatory oxysterols. The binding protein from cultured mouse fibroblasts (L cells) has been partially purified and characterized. Changes in its molecular form occur when a ligand is bound and further changes in form and binding kinetics occur at acid pH and in the presence of urea. Based on these changes a subunit model for the binding protein is presented.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Taylor FR,Kandutsch AAdoi
10.1016/0009-3084(85)90066-0subject
Has Abstractpub_date
1985-08-30 00:00:00pages
187-94issue
1-2eissn
0009-3084issn
1873-2941pii
0009-3084(85)90066-0journal_volume
38pub_type
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
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更新日期:1998-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2013.12.010
更新日期:2014-02-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2017.11.003
更新日期:2018-01-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
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