Abstract:
:Fluorescence measurements of 1,6-diphenyl-1,3,5-hexatriene (DPH) in large unilamellar phospholipid vesicles were performed to characterize the influence of the membrane physical properties on the short-lived lifetime component of the fluorescence decay. We have found that the short-lived component of DPH significantly shortens when the membrane undergoes a temperature-induced phase transition as it is known for the long-lived component of DPH. We induced membrane phase transitions also by alcohols, which are reported to be distributed different way in the membrane--ethanol close to the membrane-water interface and benzyl alcohol in the membrane core. A different effect of the respective alcohol on the short and long decay component was observed. Both the time-resolved fluorescence spectra of DPH taken during lipid vesicle staining and the lifetime dependences caused by changes of temperature and/or induced by the alcohols show that the short-lived fluorescence originates from the population of dye molecules distributed at the membrane-water interface.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Konopásek I,Vecer J,Strzalka K,Amler Edoi
10.1016/j.chemphyslip.2004.02.005subject
Has Abstractpub_date
2004-07-01 00:00:00pages
135-44issue
2eissn
0009-3084issn
1873-2941pii
S0009308404000271journal_volume
130pub_type
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