Abstract:
:Divalent cation (Ca2+ and Mg2+) and hydrogen ion-induced fusions of small unilamellar phosphatidic acid vesicles were studied by the use of fluorescence fusion assay. These fusogenic ions also increased interfacial tension and reduced the surface dielectric constant of phosphatidic acid membranes as the ion concentration increased. A good correlation was found between the threshold of vesicle fusion and the degree of changes in such membrane properties induced by these fusogenic ions. At the fusion threshold point, the increase in interfacial tension and the decreased surface dielectric constant of the membrane were approximately 6 and 14 dyn/cm, respectively, regardless of Ca2+, Mg2+ or H+. Such changes correspond to the increases in surface hydrophobicity of the membrane. As these fusogenic ionic concentrations increased, the electrophoretic mobility of the phosphatidic acid vesicle decreased, because of the binding of ions to negatively charged sites of the membranes. However, the quantities of the reduced negative surface charges were not necessarily in good correlation with the threshold of vesicle fusion. It is suggested that the complexes of the phosphate group with these fusogenic ions are responsible for increases in the surface hydrophobicity of the membrane, which is an important factor for membrane fusion.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Ohki S,Zschörnig Odoi
10.1016/0009-3084(93)90017-wsubject
Has Abstractpub_date
1993-10-01 00:00:00pages
193-204issue
3eissn
0009-3084issn
1873-2941pii
0009-3084(93)90017-Wjournal_volume
65pub_type
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journal_title:Chemistry and physics of lipids
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更新日期:2014-10-01 00:00:00
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journal_title:Chemistry and physics of lipids
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更新日期:2014-01-01 00:00:00
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journal_title:Chemistry and physics of lipids
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更新日期:2007-12-01 00:00:00
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更新日期:2008-04-01 00:00:00
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journal_title:Chemistry and physics of lipids
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pub_type: 杂志文章,评审
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更新日期:1993-09-01 00:00:00
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更新日期:2018-07-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
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更新日期:1991-03-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:1993-09-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2017.02.003
更新日期:2017-04-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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更新日期:1992-11-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/0009-3084(91)90084-o
更新日期:1991-03-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(88)90001-1
更新日期:1988-12-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2008.03.001
更新日期:2008-06-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(90)90080-b
更新日期:1990-08-01 00:00:00
abstract::Phospholipases are ubiquitous in nature and the target of significant research aiming at both their physiological roles and technical applications in e.g. the food industry. In the search for sensitive and selective phospholipase assays, we have focused on synthetic FRET (Förster resonance energy transfer) substrates....
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2016.12.002
更新日期:2017-01-01 00:00:00