Serum metabolomics analysis for early detection of colorectal cancer.


BACKGROUND:Although colorectal cancer (CRC) is one of the most common causes of cancer mortality, early-stage detection improves survival rates dramatically. Because cancer impacts important metabolic pathways, the alteration of metabolite levels as a potential biomarker of early-stage cancer has been the focus of many studies. Here, we used CE-TOFMS, a novel and promising method with small injection volume and high resolution, to separate and detect ionic compounds based on the different migration rates of charged metabolites in order to detect metabolic biomarkers in patients with CRC. METHODS:A total of 56 patients with CRC (n = 14 each of Stages I-IV), 60 healthy controls, and 59 patients with colonic adenoma were included in this study. Metabolome analysis was conducted by CE-TOFMS on serum samples of patients and controls using the Advanced Scan package (Human Metabolome Technologies). RESULTS:We obtained 334 metabolites in the serum, of which 139 were identified as known substances. Among these 139 known metabolites, 16 were correlated with CRC stage by upregulation and 44 by downregulation, with benzoic acid (r = -0.649, t = 11.653, p = 6.07599E-24), octanoic acid (r = 0.557, t = 9.183, p = 7.9557E-17), decanoic acid (r = 0.539, t = 8.749, p = 1.24352E-15), and histidine (r = -0.513, t = 8.194, p = 3.90224E-14) exhibiting significant correlation. CONCLUSIONS:To the best of our knowledge, this is the first report to determine the correlation between serum metabolites and CRC stage using CE-TOFMS. Our results show that benzoic acid exhibited excellent diagnostic power and could potentially serve as a novel disease biomarker for CRC diagnosis.


J Gastroenterol


Uchiyama K,Yagi N,Mizushima K,Higashimura Y,Hirai Y,Okayama T,Yoshida N,Katada K,Kamada K,Handa O,Ishikawa T,Takagi T,Konishi H,Kuriu Y,Nakanishi M,Otsuji E,Itoh Y,Naito Y




Has Abstract


2017-06-01 00:00:00














  • STAT3 signaling within hepatocytes is required for anemia of inflammation in vivo.

    abstract:BACKGROUND:Anemia of inflammation, commonly observed in patients with chronic diseases, is associated with decreased serum iron. Hepcidin, mainly produced by hepatocytes in a STAT3- and/or SMAD-dependent manner, is involved in iron homeostasis. What remains to be established is whether or not the hepatic IL-6/STAT3 sig...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Sakamori R,Takehara T,Tatsumi T,Shigekawa M,Hikita H,Hiramatsu N,Kanto T,Hayashi N

    更新日期:2010-02-01 00:00:00

  • Long-term follow-up study of Crohn's disease in Japan. The Research Committee of Inflammatory Bowel Disease in Japan.

    abstract::Two hundred and three patients with Crohn's disease seen at Kyushu University, Fukuoka University, and their affiliated centers from 1973 to 1988 were followed for 4.4 +/- 3.2 years (mean +/- SD), to evaluate the prognosis relative to life span and surgery. Cumulative survival rate and cumulative operation rate were c...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章,多中心研究


    authors: Iida M,Yao T,Okada M

    更新日期:1995-11-01 00:00:00

  • Vector manometric study of the sphincter of Oddi in the dog: functional and morphological correlation.

    abstract::The relationship between sphincter of Oddi pressure and the morphological structure of the sphincter was studied in eight dogs prepared with a duodenal cannula. Sphincter of Oddi manometry was performed in awake animals in three directions, ventral, left dorsal, and right dorsal, using a catheter with three radial sid...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Takeda T,Tohma H,Yoshida J,Naritomi G,Konomi H,Deng ZL,Kinukawa N,Tanaka M

    更新日期:1998-12-01 00:00:00

  • Comparison of standard-dose and low-dose gemcitabine regimens in pancreatic adenocarcinoma patients: a prospective randomized trial.

    abstract:BACKGROUND:A prospective, randomized study was performed to determine whether gemcitabine infusion at a low dose (250 mg/m2) is comparable or superior to the standard-dose infusion (1000 mg/m2) in terms of the survival period, clinical benefit, and frequency of adverse effects in patients with advanced pancreatic adeno...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章,随机对照试验


    authors: Sakamoto H,Kitano M,Suetomi Y,Takeyama Y,Ohyanagi H,Nakai T,Yasuda C,Kudo M

    更新日期:2006-01-01 00:00:00

  • Surveillance programmes for neoplasia in colitis.

    abstract::Patients with longstanding extensive colitis, both ulcerative colitis and Crohn's colitis, have an increased risk of developing colorectal cancer. Because of this risk, colonoscopic surveillance for neoplasia is recommended by most authorities. Although there has been no randomised controlled trial to demonstrate surv...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Rutter MD

    更新日期:2011-01-01 00:00:00

  • Gastrointestinal stem cells in self-renewal and cancer.

    abstract::The gastrointestinal epithelium is a unique model for the study of mammalian stem cells. Not only does it have a highly stereotypical organization, its remarkable rate of self-renewal provides a daily readout of stem cell activity. The past decade has seen a major investment in developing technologies dedicated to rev...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Lin SA,Barker N

    更新日期:2011-09-01 00:00:00

  • Nitric oxide: a regulator of mucosal defense and injury.

    abstract::There is an abundance of evidence that nitric oxide plays a critical role in regulating several components of gastrointestinal mucosal defense. Suppression of nitric oxide synthesis increases susceptibility to injury, while administration of nitric oxide donors increases resistance to injury. On the other hand, nitric...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Elliott SN,Wallace JL

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  • Serum leptin level can be a negative marker of hepatocyte damage in nonalcoholic fatty liver.

    abstract:BACKGROUND:The aim of this study was to determine whether leptin and insulin resistance (IR) showed differences between steatotic patients with and without elevated serum transaminases. METHODS:The study included 32 patients with fatty liver and high serum transaminase level (group I), 31 patients with fatty liver and...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Serin E,Ozer B,Gümürdülü Y,Kayaselçuk F,Kul K,Boyacioğlu S

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  • Correlations between lymph node metastasis and depth of submucosal invasion in submucosal invasive colorectal carcinoma: a Japanese collaborative study.

    abstract:BACKGROUND:Depth of submucosal invasion (SM depth) in submucosal invasive colorectal carcinoma (SICC) is considered an important predictive factor for lymph node metastasis. However, no nationwide reports have clarified the relationship between SM depth and rate of lymph node metastasis. Our aim was to investigate the ...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章,多中心研究


    authors: Kitajima K,Fujimori T,Fujii S,Takeda J,Ohkura Y,Kawamata H,Kumamoto T,Ishiguro S,Kato Y,Shimoda T,Iwashita A,Ajioka Y,Watanabe H,Watanabe T,Muto T,Nagasako K

    更新日期:2004-06-01 00:00:00

  • Spontaneous regression of focal nodular hyperplasia of the liver.

    abstract::Focal nodular hyperplasia (FNH) of the liver is a rare benign lesion that probably reflects a local hyperplastic response of hepatocytes to a vascular abnormality. Currently, the natural history of the disease remains largely unknown. We present a patient with FNH of the liver who was followed up for 4 years. A 22-yea...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Ohmoto K,Honda T,Hirokawa M,Mitsui Y,Iguchi Y,Kuboki M,Yamamoto S

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  • Cardiac metastasis from squamous cell carcinoma of gallbladder.

    abstract::A 65-year-old woman was admitted to our hospital with the diagnosis of gallbladder tumor. Right extended hepatic lobectomy plus lymph node dissection of the hepatoduodenal ligament and left hepaticojejunostomy with Roux-en-Y reconstruction was performed in July, 1993. The gallbladder tumor was histologically proven to...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Suganuma M,Marugami Y,Sakurai Y,Ochiai M,Hasegawa S,Imazu H,Matsubara T,Funabiki T,Kuroda M

    更新日期:1997-12-01 00:00:00

  • Effects of epidermal growth factor and insulin on migration and proliferation of primary cultured rabbit gastric epithelial cells.

    abstract::We assessed the influence of epidermal growth factor (EGF) and insulin on gastric epithelial restoration in vitro. Rabbit gastric epithelial cells were cultured and formed a complete monolayer cell sheet in 2 days. We created a wound (1.8 +/- 0.05 mm2) by denuding an area of cells, and EGF (0.1-30 ng/ml) and/or insuli...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Maehiro K,Watanabe S,Hirose M,Iwazaki R,Miwa H,Sato N

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  • Loss of diurnal variation in ornithine decarboxylase and apoptosis in small intestine of Mongolian gerbils.

    abstract::The aim of this study was to examine the diurnal variations in ornithine decarboxylase (ODC) activity and apoptosis in the small intestinal mucosa of Mongolian gerbils. First, the feeding, drinking, and ambulatory patterns of the gerbils were recorded. The results indicated that Mongolian gerbils lost diurnal cyclicit...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Kashiwagi Y,Fujimoto K,Iwakiri R,Yoshida T,Noda T,Aw TY,Niho Y,Sakata T

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  • Resection depth and layer of cold snare polypectomy versus endoscopic mucosal resection.

    abstract:BACKGROUND:Cold snare polypectomy (CSP) has not undergone sufficient histopathological evaluation. This study aimed to clarify the histopathological features of CSP specimens, including resection depth and layer, as compared with endoscopic mucosal resection (EMR). METHODS:Polyps were recruited retrospectively. Sessil...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Ito A,Suga T,Ota H,Tateiwa N,Matsumoto A,Tanaka E

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  • Intrahepatic cholangiocarcinoma with increased serum CYFRA 21-1 level.

    abstract::CYFRA 21-1 is a fragment of cytokeratin 19 (CK 19). Four patients with large intrahepatic (or peripheral) cholangiocarcinoma (CC) and high serum levels of CYFRA 21-1 (normal, < or = 2 ng/ml) are reported. CYFRA 21-1 levels exceeded 9 ng/ml in all 4 patients. Carcinoembryonic antigen (CEA), was high in 1 (CEA; normal r...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Kashihara T,Ohki A,Kobayashi T,Sato T,Nishizawa H,Ogawa K,Tako H,Kawakami F,Tsuji M,Tamaoka K

    更新日期:1998-06-01 00:00:00

  • Epidemiological study of gastroenteropancreatic neuroendocrine tumors in Japan.

    abstract:BACKGROUND:There have been few epidemiological studies on gastroenteropancreatic neuroendocrine tumors (GEP-NETs) in Japan. METHODS:We examined the epidemiology of GEP-NETs [pancreatic endocrine tumors (PETs) and gastrointestinal neuroendocrine tumors (GI-NETs)] in Japan in 2005 using a nationwide stratified random sa...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Ito T,Sasano H,Tanaka M,Osamura RY,Sasaki I,Kimura W,Takano K,Obara T,Ishibashi M,Nakao K,Doi R,Shimatsu A,Nishida T,Komoto I,Hirata Y,Nakamura K,Igarashi H,Jensen RT,Wiedenmann B,Imamura M

    更新日期:2010-02-01 00:00:00

  • Longitudinal association of fatty pancreas with the incidence of type-2 diabetes in lean individuals: a 6-year computed tomography-based cohort study.

    abstract:BACKGROUND:Only a few studies have longitudinally evaluated whether fatty pancreas increases the risk of type-2 diabetes (T2D), and their results were inconsistent. Fatty pancreas is closely linked to overweight and obesity, but previous studies did not exclude overweight or obese individuals. Therefore, in this cohort...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Yamazaki H,Tauchi S,Wang J,Dohke M,Hanawa N,Kodama Y,Katanuma A,Saisho Y,Kamitani T,Fukuhara S,Yamamoto Y

    更新日期:2020-07-01 00:00:00

  • Influence of anti-ulcer drugs used in Japan on the result of (13)C-urea breath test for the diagnosis of Helicobacter pylori infection.

    abstract:BACKGROUND:The (13)C-urea breath test (UBT) is a simple breath test for the diagnosis of Helicobacter pylori infection, but several factors have been reported to affect the results of this test. In this study, the effects of the antiulcer drugs used in Japan on the results of UBT were determined. METHODS:The subjects ...

    journal_title:Journal of gastroenterology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Murakami K,Sato R,Okimoto T,Watanabe K,Nasu M,Fujioka T,Kodama M,Kagawa J

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  • Recent clinical features of Wilson's disease with hepatic presentation.

    abstract:BACKGROUND:We carried out this study to evaluate recent clinical features of Wilson's disease (WD) with hepatic presentation, especially in terms of age, degree of liver injury, and association with hepatocellular carcinoma (HCC). METHODS:Sixteen patients with hepatic manifestations were diagnosed with WD in the perio...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Kumagi T,Horiike N,Michitaka K,Hasebe A,Kawai K,Tokumoto Y,Nakanishi S,Furukawa S,Hiasa Y,Matsui H,Kurose K,Matsuura B,Onji M

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  • Histological evaluation of obliterative phlebitis for the diagnosis of autoimmune pancreatitis.

    abstract:BACKGROUND:Obliterative phlebitis is a useful pathological finding for the diagnosis of lymphoplasmacytic sclerosing pancreatitis (LPSP), or type 1 autoimmune pancreatitis. The present study evaluated histological findings of obliterative phlebitis, including the significance of adding Elastica van Gieson stain (EVG) i...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Miyabe K,Notohara K,Nakazawa T,Hayashi K,Naitoh I,Okumura F,Shimizu S,Yoshida M,Yamashita H,Takahashi S,Ohara H,Joh T

    更新日期:2014-04-01 00:00:00

  • Pancreatic ductal adenocarcinoma associated with Potter type III cystic disease.

    abstract::Although polycystic liver disease (PLD) is known to be associated with autosomal dominant polycystic kidney disease, a finding of PLD with pancreatic ductal adenocarcinoma is extremely rare. We have experienced one such case of a ductal adenocarcinoma of the pancreas in a patient with Potter type III cystic disease of...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Sakurai Y,Shoji M,Matsubara T,Ochiai M,Funabiki T,Urano M,Mizoguchi Y,Fuwa N

    更新日期:2001-06-01 00:00:00

  • The effect of etanercept on hepatic fibrosis risk in patients with non-alcoholic fatty liver disease, metabolic syndrome, and psoriasis.

    abstract:BACKGROUND:Patients with psoriasis show a greater prevalence of non-alcoholic fatty liver disease (NAFLD) and metabolic syndrome than the general population. Moreover, patients with NAFLD and psoriasis are at higher risk of severe liver fibrosis than their counterparts with NAFLD and without psoriasis. The link between...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Campanati A,Ganzetti G,Di Sario A,Damiani A,Sandroni L,Rosa L,Benedetti A,Offidani A

    更新日期:2013-07-01 00:00:00

  • Effects of submucosal administration of endothelin-3 on rat gastric mucosa.

    abstract::The present study was carried out to examine the effects of submucosal administration of endothelin on gastric mucosal integrity in rats. Injection of endothelin-3 into the submucosal space of the stomach induced gastric mucosal damage dose-dependently and site-specifically. The gastric injury was localized only at th...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Watanabe Y,Okumura T,Harada K,Kohgo Y

    更新日期:1997-04-01 00:00:00

  • Computed tomography in the diagnosis of adult midgut rotational anomalies: a report of two cases.

    abstract::Adult midgut rotational anomalies are difficult to diagnose. Any symptoms present in adults may be the result of intermittent volvulus of the midgut. Successful outcome of this clinical condition depends on prompt diagnosis and timely surgical intervention. Computed tomography (CT) is useful in early identification of...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Wang TK,Yeh CH

    更新日期:1998-02-01 00:00:00

  • Pancreas head carcinoma with total fat replacement of the dorsal exocrine pancreas.

    abstract::We report a case of pancreas head carcinoma associated with fat replacement of the body and tail. A 68-year-old man presented with obstructive jaundice and was admitted to our hospital. Ultrasonography and computed tomography showed pancreas head tumor with a neighboring cystic lesion and fatty replacement of parenchy...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Toyama N,Kamiyama H,Suminaga Y,Namai K,Ota M,Konishi F

    更新日期:2004-01-01 00:00:00

  • Characterization of polyethylene glycol-grafted polyethylenimine and superparamagnetic iron oxide nanoparticles (PEG-g-PEI-SPION) as an MRI-visible vector for siRNA delivery in gastric cancer in vitro and in vivo.

    abstract:BACKGROUND:Gene therapy is a promising therapeutic method but is severely hampered due to its lack of an ideal delivery system. Therefore, in this study, a nonviral and magnetic resonance imaging (MRI) visible vector, polyethylene glycol-grafted polyethylenimine and superparamagnetic iron oxide nanoparticles (PEG-g-PEI...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Chen Y,Lian G,Liao C,Wang W,Zeng L,Qian C,Huang K,Shuai X

    更新日期:2013-07-01 00:00:00

  • Circular RNA TMEM87A promotes cell proliferation and metastasis of gastric cancer by elevating ULK1 via sponging miR-142-5p.

    abstract:BACKGROUND:Circular RNAs (circRNAs) act as vital regulators of gene expression in a variety of cancers. However, the role of circRNAs in gastric cancer (GC) remains largely unexplored. Herein, we identified that circTMEM87A sponges miR-142-5p to promote GC progression through up-regulating ULK1 expression. METHODS:The...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Wang H,Sun G,Xu P,Lv J,Zhang X,Zhang L,Wang S,Cao J,Xia Y,Xuan Z,Li B,Huang X,Jiang T,Fang L,Xu Z

    更新日期:2020-11-06 00:00:00

  • Significance of variants associated with resistance to NS5A inhibitors in Japanese patients with genotype 1b hepatitis C virus infection as evaluated using cycling-probe real-time PCR combined with direct sequencing.

    abstract:BACKGROUND:Dual oral therapy with daclatasvir plus asunaprevir yielded an SVR rate of 85% among patients with genotype 1b HCV. Treatment failure mainly occurred in patients with pre-existing HCV with NS5A-Y93H mutation. The significance of the mutation was evaluated. METHODS:The percent of serum NS5A-Y93H strains rela...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Uchida Y,Kouyama J,Naiki K,Sugawara K,Ando S,Nakao M,Motoya D,Inao M,Imai Y,Nakayama N,Mochida S

    更新日期:2016-03-01 00:00:00

  • Nationwide epidemiological survey of autoimmune pancreatitis in Japan in 2016.

    abstract:BACKGROUND:To further clarify the clinico-epidemiological features of autoimmune pancreatitis (AIP) in Japan, we conducted the fourth nationwide epidemiological survey. METHODS:This study consisted of two stage surveys; the number of AIP patients was estimated by the first survey and their clinical features were asses...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Masamune A,Kikuta K,Hamada S,Tsuji I,Takeyama Y,Shimosegawa T,Okazaki K,Collaborators.

    更新日期:2020-04-01 00:00:00

  • Nationwide epidemiological survey of chronic pancreatitis in Japan: introduction and validation of the new Japanese diagnostic criteria 2019.

    abstract:OBJECTIVES:To provide updated clinico-epidemiological information on chronic pancreatitis (CP) in Japan. METHODS:We conducted a two-stage nationwide epidemiological survey; the number of CP patients was estimated in the first-stage survey, and their clinical features were examined in the second-stage survey. We survey...

    journal_title:Journal of gastroenterology

    pub_type: 杂志文章


    authors: Masamune A,Kikuta K,Kume K,Hamada S,Tsuji I,Takeyama Y,Shimosegawa T,Okazaki K,Japan Pancreas Society.

    更新日期:2020-11-01 00:00:00