Abstract:
CONTEXT:Velvet antler (VA) is recognized as one of the most important Chinese traditional medical herbs. To date, the immunoactivity of the single component of VA is rarely studied though its compound extracts have been well analyzed. OBJECTIVE:The current study was designed to evaluate the immunomodulatory effects of a recombinant polypeptide (rVAP32) based on the VA of the sika deer by comparison with its natural counterpart (nVAP32). MATERIALS AND METHODS:Splenocytes proliferation and NK-cell cytotoxicity assay was evaluated by the WST-8 colorimetric method. CD4+/CD8+ cell subpopulations regulation was screened by the flowcytometry method and the Th1 or Th2-related cytokine production was measured by ELISA. RESULTS:In vitro tests showed that both rVAP32 and nVAP32 could significantly stimulate splenocytes proliferation and enhance the NK-cell cytotoxicity and CD4+/CD8+ cell subpopulations when compared with the irrelevant peptide and blank control groups. Also, they demonstrated a significant capacity in up- and down-regulating the expression of Th1- and Th2-related cytokines, respectively. There is no statistically significant difference found between the rVAP32 tested group and nVAP32 control group. DISCUSSION AND CONCLUSION:The results obtained herein indicate that rVAP32 has the similar immunomodulatory effects on the immune system of mice as its counterpart nVAP32 in vitro. The further test in vivo is qualified and rVAP32 is promised for developing a new biopharmaceutical product as a substitute for nVAP32.
journal_name
Immunopharmacol Immunotoxicoljournal_title
Immunopharmacology and immunotoxicologyauthors
Zha E,Dandan L,Bai X,Zhou T,Li Y,Shenyang G,Yue Xdoi
10.1080/08923973.2016.1233978subject
Has Abstractpub_date
2016-12-01 00:00:00pages
385-389issue
6eissn
0892-3973issn
1532-2513journal_volume
38pub_type
杂志文章abstract::Contribution of cytotoxic T lymphocytes (CTL) to experimental autoimmune thyroiditis (EAT) was well defined (Speidel et al., Eur. J. Immunol. 1997, 27, 2391-2399, Ref. 7). The native porcine thyroglobulin (pTg) showed high sensitivity to endo-o-N-acetylglucosaminidase F (Endo F) and its molecular weights, correspondin...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章,收录出版
doi:10.1081/iph-200026872
更新日期:2004-08-01 00:00:00
abstract::Context: Tamoxifen (TAM) ameliorates D-galactosamine/lipopolysaccharide (Gal/LPS)-induced acute liver failure (ALF) through its antioxidative effect; thus, this study was designed to determine whether the effectiveness of TAM is related to nuclear factor-κB (NF-κB) reactivation. Materials and methods: Experimental mic...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2019.1569044
更新日期:2019-04-01 00:00:00
abstract::The induction of T-cell responses involves the recognition of extrinsic antigens in association with antigens of the major histocompatibility complex (MHC). The present results demonstrate that the lateralized control exerted by the brain neocortex on T-cell activities extends to the expression of MHC antigens, yet di...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923978809014334
更新日期:1988-01-01 00:00:00
abstract::Protective effect of a Japanese traditional herbal medicine, Juzen-taiho-to (TJ-48), which was recently reported to augment host-mediated antifungal actions, in Candida albicans-infected mice was further studied. TJ-48, given orally once daily for 5 consecutive days in a dose of 2 g/kg after intravenous infection of C...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979609007111
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abstract::Langerhans cells (LC) are immature dendritic cells (DC) present in the skin epithelium. To understand the molecular and cellular mechanisms governing the inflammatory reaction in atopic dermatitis (AD), the expression of the LC specific marker CD1a, a member of major histocompatibility (MHC)-like glycoproteins, and th...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970701675101
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abstract::Myricetin, a common dietary flavonoid, is widely distributed in fruits and vegetables. It is known to be a food supplement contributing to human health because of its immune modulatory function, and its antioxidation, antitumor, and anti-inflammatory properties. In the present study, myricetin was shown to directly in...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2015.1096942
更新日期:2015-01-01 00:00:00
abstract::Host-parasite interactions and their outcome constitute a critical and challenging step in disease establishment in cutaneous leishmaniasis. In the present in vitro study we investigated the possible modulating effects of both sensory and autonomic neuropeptides that normally exist in human and mouse skin, on the upta...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-100107339
更新日期:2001-08-01 00:00:00
abstract::Ethanol has been recognized as an immunosuppressive agent for many years. Effects of high levels of ethanol consumption on immune functions have been extensively studied, but little is known about the effects of low levels (scuh as 5% ethanol) of ethanol consumption. Herein we report that exposure of mice to 5% ethano...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120003408
更新日期:2002-02-01 00:00:00
abstract::Objective: Apigenin is a natural flavonoid compound extracted from Matricaria chamomilla. We evaluated the anti-inflammatory effects of apigenin in this study using the Lipopolysaccharide (LPS)-stimulated BV2 microglia.Methods: BV2 cells were treated with apigenin for 1 h and then treated with LPS. The inflammatory cy...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923973.2019.1688345
更新日期:2020-02-01 00:00:00
abstract::The effects of anti-thyroid drug, methimazole (MMI), on haematopoiesis in inbred C57BL/6 mice were studied. The in vitro proliferative response of bone marrow (BM) cells to interleukin-3 (IL-3) was significantly increased when mice were provided with 0.1% MMI in water (w/v) ad librium for 4 to 6 weeks. Using soft agar...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979509037193
更新日期:1995-11-01 00:00:00
abstract::Arecoline, a major alkaloid of arecanut was screened to explore its modulatory influence on cell-mediated immune response in a murine model system. The in vivo and in vitro effects were evaluated at subtoxic concentrations of arecoline. Delayed type hypersensitivity (DTH) reactions to sheep red blood cells (SRBC) were...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979109019706
更新日期:1991-01-01 00:00:00
abstract::The protective effects of baicalin (BA), a major flavone from Scutellaria radix, on acetaminophen (AP)-induced hepatotoxicity and the possible mechanism(s) of its protective action were investigated in mice. Treatment with BA (300 mg/kg, p.o.) 0.5 h after AP administration significantly prevented an increase in plasma...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120026443
更新日期:2003-11-01 00:00:00
abstract::Recently, a number of studies have focused on the secondary effects of rifampicin. In the present study, we assessed whether rifampicin influences the rapamycin-induced autophagy of RAW 264.7 cells. Here, we demonstrate that the rapamycin-induced autophagy is dependent on protein phosphatase (PP) 2A activity and rifam...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970802135732
更新日期:2008-01-01 00:00:00
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journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923973.2012.685630
更新日期:2012-12-01 00:00:00
abstract:CONTEXT:Disease Modifying Anti-Rheumatic Drugs (DMARDs) are aimed to interfere with rheumatoid arthritis (RA) progression and reduce the joint damage; however, not all patients respond alike. Killer-cell immunoglobulin-like receptors (KIR) and their ligands, human leucocyte antigen class I (HLA-I), have been associated...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 临床试验,杂志文章
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更新日期:2016-08-01 00:00:00
abstract::The recruitment of eosinophils from the circulation into the airway is a prominent feature of allergic asthma. Persistent inflammatory responses may arise from inefficient mechanisms for resolution of inflammation, including delayed apoptosis. Several studies suggest that eosinophil apoptosis is delayed in asthma. Sia...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章,评审
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abstract::The aim of the present investigation was to evaluate the efficacy of Withania somnifera on tricarboxylic acid (TCA) cycle enzymes and electron transport chain in azoxymethane-induced experimental colon cancer in mice. Azoxymethane at the dose of 15 mg/kg body weight was induced intraperitoneally once in a week for 28 ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923970903581540
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abstract:OBJECTIVES:Abnormal microglia secrete neuroinflammatory factors that play a pivotal role in neurodegenerative-disorder development. Thus, regulating abnormal microglia-activation could be a promising therapeutic strategy. The purposes of this study included investigating the effect of Petatewalide B on lipopolysacchari...
journal_title:Immunopharmacology and immunotoxicology
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doi:10.1080/08923973.2018.1434791
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abstract::Several abnormalities of the immune system have been reported in association with clinical and experimental iron overload. To dissect further such abnormalities, changes in lymphocyte subsets were evaluated in iron-loaded male Sprague-Dawley rats. The iron-loading protocol consisted of a total dose of irondextran (1.5...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979709038534
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abstract::Atractylenolide I (ATL-I) is a bioactive component of Rhizoma Atractylodis macrocephalae. Although increasing evidence shows that ATL-I has an anti-inflammatory effect, the anti-inflammatory molecular mechanism of ATL-I is still unknown. In this study, we investigated the effect of ATL-I on cell viability by 3-(4, 5-D...
journal_title:Immunopharmacology and immunotoxicology
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abstract::The Scrophularia buergeriana (SB) has long been used to treat various diseases an account of its antimicrobial and anti-virus activity. However, it is unclear how SB regulates the immune responses. This study investigated the effect of SB on the production of cytokines in a human T-cell line, MOLT-4 cells, and mouse p...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1080/08923970802432048
更新日期:2009-06-01 00:00:00
abstract::Natural killer (NK) activity plays an important role in host defense against tumors, especially once augmented by immunomodulators. It is likely that the modulation of NK cells is a reflection of the environment in which they reside. The current study was undertaken to characterize the response profile of lung interst...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923979109019703
更新日期:1991-01-01 00:00:00
abstract::We previously showed that formation of pulmonary granulomas in mice in response to a mycobacterial glycolipid, trehalose 6,6'-dimycolate (TDM) is due to the action of TNF-α and not of IFN-γ. However, the mechanisms of formation and maintenance of pulmonary granulomas are not yet clear. The purpose of the present study...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2012.658922
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journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.3109/08923973.2012.697470
更新日期:2012-12-01 00:00:00
abstract::The aim of the present study was to investigate putative mechanisms implicated in the impaired phagocytic response of spontaneously hypertensive rats (SHR)1. The effect of in vitro treatment with isoproterenol (ISO), a beta-adrenergic drug, on phagocytosis and respiratory burst by splenic macrophages (SpMø) from normo...
journal_title:Immunopharmacology and immunotoxicology
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doi:10.3109/08923979609052735
更新日期:1996-05-01 00:00:00
abstract:OBJECTIVE:Ipomoea obscura (L.) Ker-Gawl, is a medicinal herb with indole alkaloids as an active constituent. In this study, we investigated the anti-angiogenic activity of I. obscura extract and one of its major compounds Ipobscurine-A (IPO-A). METHODS:In vivo angiogenesis was induced by injecting B16F10 melanoma cell...
journal_title:Immunopharmacology and immunotoxicology
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更新日期:2011-09-01 00:00:00
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journal_title:Immunopharmacology and immunotoxicology
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abstract::Association between Crohn's disease (CD) and lupus nephritis is very rare and, to the best of our knowledge, it has been described only once. We report here a clinical case of CD occurred in a young woman 8 years after a diagnosis of lupus nephritis according to clinical, laboratory and histological criteria. CD was u...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
doi:10.1081/iph-120037721
更新日期:2004-05-01 00:00:00
abstract::To investigate the role of CD4(+) T cells in neurotrophin production following toluene exposure, male C3H mice were exposed to filtered air (control) or 9 ppm of toluene in a nose-only exposure chamber for 30 min on 3 consecutive days followed by weekly sessions for 4 weeks. All the mice were immunized with ovalbumin ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
abstract::In this study we investigated the ability of GM-1/P, a calcium mediated processed form of monosialoganglioside GM-1, of in vivo augmenting mouse T and B-lymphocyte blastogenesis induced by mitogens. We have also determined its effect on IL-2 responsiveness by analyzing the induction of the expression of IL-2 receptor ...
journal_title:Immunopharmacology and immunotoxicology
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00