Abstract:
BACKGROUND AND OBJECTIVES:Helicobacter pylori (H. pylori) infection is cause of several gastrointestinal diseases in humans. Virulence genes of H. pylori are associated with severity of disease and vary geographically. The aim of present study was to detect H. pylori in formalin-fixed paraffin-embedded (FFPE) tissues and further investigate prevalence of babA2, cagA, iceA1, iceA2, vacA s1/s2 and vacA m1/m2 genotypes in H. pylori from gastric cancer (GC) and gastric ulcer (GU) patients' biopsy samples. METHODS:We used FFPE tissues of 35 GC and 10 GU patients' biopsy samples. Using Polymerase Chain Reaction (PCR), detection of H. pylori strain was performed by using specific primers targeting 16S rRNA and ureC encodes for phosphoglucosamine mutase genes. We have identified different virulence genes of H. pylori by PCR. RESULTS:Of all the 45 samples tested, 20 GC and all 10 GU samples were positive for identification of H. pylori using specific genes (16S rRNA and ureC). The prevalence of babA2 (100%) was significantly higher in GC as compared to GU (40%) samples. The rate of virulence genes vacAs1 was higher in both GU 8 (80%) and GC (100%). CONCLUSIONS:Our study finds that vacAs1am1 and babA2 are most prominent genotypes and may play role in development of Gastric cancer.
journal_name
Pak J Med Scijournal_title
Pakistan journal of medical sciencesauthors
Bibi F,Alvi SA,Sawan SA,Yasir M,Sawan A,Jiman-Fatani AA,Azhar EIdoi
10.12669/pjms.332.12024subject
Has Abstractpub_date
2017-03-01 00:00:00pages
320-324issue
2eissn
1682-024Xissn
1681-715Xpii
PJMS-33-320journal_volume
33pub_type
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.325.11217
更新日期:2016-09-01 00:00:00
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更新日期:2014-01-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.35.6.1322
更新日期:2019-11-01 00:00:00
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pub_type: 杂志文章
doi:10.12669/pjms.345.15418
更新日期:2018-09-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章,评审
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更新日期:2020-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-05-01 00:00:00
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pub_type: 杂志文章
doi:10.12669/pjms.35.2.334
更新日期:2019-03-01 00:00:00
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doi:10.12669/pjms.344.14391
更新日期:2018-07-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.341.14354
更新日期:2018-01-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.345.15224
更新日期:2018-09-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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更新日期:2016-05-01 00:00:00
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pub_type: 杂志文章
doi:10.12669/pjms.37.1.3721
更新日期:2021-01-01 00:00:00
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doi:10.12669/pjms.35.2.679
更新日期:2019-03-01 00:00:00
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pub_type: 杂志文章
doi:10.12669/pjms.344.14602
更新日期:2018-07-01 00:00:00
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journal_title:Pakistan journal of medical sciences
pub_type: 杂志文章
doi:10.12669/pjms.36.3.541
更新日期:2020-03-01 00:00:00