Prognostic markers in Chronic Lymphocytic Leukaemia - A flow cytometric analysis.

Abstract:

Objective:To find out the frequency of ZAP-70, CD38 and CD49d in patients diagnosed with CLL in our population. Methods:This is a cross sectional study conducted in Army Medical College in collaboration with Armed Forces Institute of Pathology and Military Hospital Rawalpindi from 1st January 2018 to 30th November 2018. Permission from Institutional Ethical Committee was obtained. Blood samples were collected by non-probability consecutive sampling technique and analyzed for blood counts and flow cytometry was done for ZAP-70, CD38 and CD49d. Manufacturer's instructions for the kits were strictly followed. Results:Fifty-one newly diagnosed patients with CLL were studied for the prognostic markers in CLL. CD 38 was expressed in 25(49%) and CD49d in 21(41.2%). ZAP-70 expression was not detected in our series of patients. Conclusion:We conclude that CD38 and CD49d expression was detected in almost half of the patients of CLL in our series. CD49d showed statistically positive correlation with CD38, showing that it is a more pragmatic choice for reliable prognostication of CLL along with CD38.

journal_name

Pak J Med Sci

authors

Haq H,Uddin N,Khan SA,Ghaffar S

doi

10.12669/pjms.36.3.541

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

338-343

issue

3

eissn

1682-024X

issn

1681-715X

pii

PJMS-39-338

journal_volume

36

pub_type

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