Abstract:
:Scaffolds porosity has an important role in in vitro and in vivo differentiation process of stem cells with given the amount of space available to the cells to proliferate and differentiate. In the present study, chitosan with three porosities including 10%, 15%, and 20% that created by gelatin were used for investigation of the proliferation and osteogenic differentiation potential of adipose-derived stem cells (ADSCs). In order to be more like the scaffold to natural bone tissue, freeze-drying method was used in the scaffold preparation. Scaffold morphology, cell attachment, and toxicity were evaluated using scanning electron microscopy and MTT assay. Then, osteogenic differentiation potential of ADSCs cultured on chitosan with different porosities was evaluated by common osteogenic markers such as Alizarin red staining, ALP activity, calcium content, and osteogenic-related genes expression via real-time RT-PCR. Although all scaffolds supported the proliferation and differentiation of ADSCs, but 10% scaffold demonstrated higher amount of osteogenic markers in comparison with the other porosities and control groups. Taking together, it can be concluded that osteogenic differentiation well done in the scaffolds with lower porosity because density of the cells will increase by forcing resulted from the scaffold, so osteogenic differentiation of the stem cells have an inverse association with scaffold porosity. J. Cell. Biochem. 119: 625-633, 2018. © 2017 Wiley Periodicals, Inc.
journal_name
J Cell Biochemjournal_title
Journal of cellular biochemistryauthors
Ardeshirylajimi A,Delgoshaie M,Mirzaei S,Khojasteh Adoi
10.1002/jcb.26223subject
Has Abstractpub_date
2018-01-01 00:00:00pages
625-633issue
1eissn
0730-2312issn
1097-4644journal_volume
119pub_type
杂志文章abstract::Metastatic cancer is a complex positive feedback loop system. Such as system has a tendency to acquire extreme robustness. Signaling pathways controlling that robustness can fail completely if an essential element from the signaling is removed. That element is a locus of fragility. Targeting that locus represents the ...
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