Abstract:
:Small activating RNA (saRNA)-mediated gene activation has opened a new avenue for upregulating the expression of target genes by promoting endogenous transcription, a phenomenon known as RNA activation (RNAa). RNAa is distinct from the established RNAi mechanistic framework, although AGO2 is required by both. The precise mechanism of RNAa is currently disputable and has become a bottleneck in the development of this new technology. saRNA may achieve activation of target genes by directly binding to DNA targets in promoter, or interacting with antisense transcripts transcribed from overlapping promoter sequences, or by silencing other genes. In this chapter, we focused on recent development in our understanding of the target-recognition mechanism in RNAa. Conflicting results on saRNA targets are also discussed. Despite that the target mechanism of RNAa is more complex than expected and not completely understood so far, independent lines of evidence have suggested that saRNAs work by an "on-site" mechanism by binding to target genomic DNA in a "seed-region"-dependent manner. Finally, "off-target" effects of saRNA are observed and should be carefully controlled in designing experiments for and interpreting results from RNAa-related studies.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Cao H,Meng X,Wang X,Liang Zdoi
10.1007/978-981-10-4310-9_3subject
Has Abstractpub_date
2017-01-01 00:00:00pages
41-51eissn
0065-2598issn
2214-8019journal_volume
983pub_type
杂志文章abstract::Studies have been cazrried out to determine the in vivo and in vitro effects of lithium carbonate in various neutropenic disorders. Addition of lithium to culture of bone marrow from patients with a variety of neutropenic disorders and ANLL in which normal or elevated serum and/or urinary CSA levels were present did n...
journal_title:Advances in experimental medicine and biology
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journal_title:Advances in experimental medicine and biology
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
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更新日期:2016-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
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更新日期:2016-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:1991-01-01 00:00:00