Abstract:
:AFM-based Single Molecule Force Spectroscopy provides a new tool for probing the mechanical properties of single molecules. In this chapter we show that the unfolding forces of single protein domains can be directly measured. Unfolding forces give new insight into protein stability that cannot be deduced from thermodynamic measurements. A comparison between the unfolding forces measured in Ig domains of the muscle protein titin and those measured in fibronectin Type III domains reveals an extraordinarily high stability of titin domains.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Rief M,Gautel M,Gaub HEdoi
10.1007/978-1-4615-4267-4_8subject
Has Abstractpub_date
2000-01-01 00:00:00pages
129-36; discussion 137-41eissn
0065-2598issn
2214-8019journal_volume
481pub_type
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abstract::Carbon compounds that are needed in small amounts in the diet because they are not made in the body of vertebrates are defined as vitamins. Excluded from this definition are vitamins D, K, and niacin which can be synthesized by the organism or, as in the case of vitamin K, by the host's intestinal bacteria. Lack of su...
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