Abstract：
:In order to determine the degree of mechanochemical coupling in actomyosin in vitro motility assays, it is desirable to measure the sliding velocity and the associated ATP turnover simultaneously at the single filament level. Actin sliding over tracks of immobilised heavy meromyosin (HMM) has been initiated by flash photolysis of caged ATP. Flash photolysis has also been used to displace fluorescent Cy3-EDA-nucleotides from HMM tracks to monitor the ATPase activity. These assays are now being combined using total internal reflectance fluorescence (TIRF) microscopy with a dual-view detection system for Cy3/Cy5 labels on ATP and actin respectively. In other experiments, we are exploring the use of the single molecule kinetic technique developed by Funatsu et al. (Nature 374, 555-559, 1995) to scale down ATPase assays of Dictyostelium myosin fragments and to elucidate the mechanism of regulation of the molluscan (scallop) myosin ATPase. Although fluctuations occur from the binding and release of Cy3-EDA-nucleotides during turnover and might provide a measure of the ATPase activity, other sources of fluctuations also need to be considered.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Conibear PB,Kuhlman PA,Bagshaw CRdoi
10.1007/978-1-4684-6039-1_3subject
Has Abstractpub_date
1998-01-01 00:00:00pages
15-26; discussion 26-7eissn
0065-2598issn
2214-8019journal_volume
453pub_type
杂志文章abstract：:Key autonomic functions are in continuous development during adolescence which can be assessed using the heart rate variability (HRV). However, the influence of different demographic and physiological factors on HRV indices has not been fully explored in adolescents. In this study we aimed to assess the effect of age,...
journal_title：Advances in experimental medicine and biology
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abstract：:Percutaneous vertebroplasty is a treatment option in vertebral compression fractures (VCF). The aim of the study was to propose the mathematical calculation of the "optimum volume" of acrylic cement filling of the vertebral body, depending on the severity of a fracture. Two hundred computed tomography (CT) scans of ve...
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-9373-7_19
更新日期：1984-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5679-0_24
更新日期：1989-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-94-024-1079-2_62
更新日期：2017-01-01 00:00:00
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更新日期：2010-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-319-91287-5_46
更新日期：2018-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5143-6_55
更新日期：1986-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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doi：10.1007/978-3-319-67577-0_10
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journal_title：Advances in experimental medicine and biology
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更新日期：2019-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-67358-5_4
更新日期：2017-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-48382-5_18
更新日期：2017-01-01 00:00:00
abstract：:A series of experiments compared the uptake of 5,10,15,20 tetrakis(4-carboxyphenyl) porphinato [67Cu] copper (II), 67CuTCPP, by the lymph nodes of inflamed and two sets or control rats. The results demonstrate that 67CuTCPP localizes in greater concentration in inflamed lymph nodes than in noninflamed control lymph no...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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更新日期：1989-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-0629-4_8
更新日期：2002-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-1243-1_13
更新日期：2001-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-981-13-8023-5_60
更新日期：2019-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
abstract：:The basic physical and functional unit of heredity; made up of DNA, they act as instructions to make proteins. There are about 20,000-25,000 genes in humans, with size varying from a few hundred DNA bases to more than 2 million bases. Most genes are the same in all people; less than 1% differ. Each person has two copi...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-95046-4_7
更新日期：2018-01-01 00:00:00
