Abstract：
:Ascidians possess relatively small and compact genomes. This feature enables us to easily isolate cis-regulatory DNAs of genes of interest. Particularly, cis-regulatory DNAs of genes showing tissue- or cell-type-specific expression are routinely used for the artificial induction of gene expression. This strategy helps us to label cells, tissues, and organs of interest, and to investigate gene functions through overexpression, ectopic expression, and the disruption of functions by dominant-negative forms. Thus, cis-regulatory DNAs provide a powerful tool for tissue-specific genetic manipulation in studies of ascidian development and physiology. This chapter summarizes the types of cis-regulatory DNAs as a genetic manipulation tool, describes the methods used for isolating cis-regulatory DNAs, and provide reported examples of the use of cis-regulatory DNAs as molecular tools for investigating gene functions.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Shimai K,Kusakabe TGdoi
10.1007/978-981-10-7545-2_6subject
Has Abstractpub_date
2018-01-01 00:00:00pages
49-68eissn
0065-2598issn
2214-8019journal_volume
1029pub_type
杂志文章,评审abstract：:Myocarditis is the inflammation of the muscle tissues of the heart (myocardium). After a pathologic cardiac-specific inflammatory process, it may progress to chronic damage and dilated cardiomyopathy. The latter is characterized by systolic dysfunction, whose clinical correlate is heart failure. Nevertheless, other ac...
journal_title：Advances in experimental medicine and biology
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pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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更新日期：2011-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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更新日期：1993-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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更新日期：1977-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/0-306-46840-9_20
更新日期：2000-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-1959-5_12
更新日期：1998-01-01 00:00:00
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doi：
更新日期：1997-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
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更新日期：2016-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4613-0537-8_4
更新日期：1989-01-01 00:00:00
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更新日期：2018-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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更新日期：2016-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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更新日期：1989-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Connexin 43 (Cx43) is the major connexin protein in ventricular cardiomyocytes. Six Cx43 proteins assemble into so-called hemichannels at the sarcolemma and opposing hemichannels form gap junctions, which allow the passage of small molecules and electrical current flow between adjacent cells. Apart from its localizati...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2017-01-01 00:00:00
