Development of cultures of the marine sponge Hymeniacidon perleve for genotoxicity assessment using the alkaline comet assay.

Abstract:

:Sponges are a potential alternative model species to bivalves in pollution biomonitoring and environmental risk assessment in the aquatic ecosystem. In the present study, a novel in vivo exposure sponge culture model was developed from field-collected and cryopreserved sponge (Hymeniacidon perleve) cells to investigate the genotoxic effects of environmentally relevant metals in the laboratory. Sponge cell aggregates were cultured and exposed to noncytotoxic concentrations (0-0.4 mg/L) of cadmium chloride, nickel chloride, and sodium dichromate as quantified by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and DNA-strand breaks assessed by the comet assay. Reactive oxygen species (ROS) formation was quantified by oxidation of 2',7'-dichlorofluorescin diacetate in sponge cell aggregates exposed to the same concentrations of Cd, Cr, and Ni. There was a statistically significant (p < 0.05) concentration-dependent increase in the level of DNA-strand breaks and ROS formation in all of the metals investigated. To the best of our knowledge, we have utilized for the first time the alkaline comet assay to detect DNA-strand breaks in marine sponge cells and demonstrated that exposure to noncytotoxic concentrations of Cd, Cr, and Ni for 12 h results in a concentration-dependent increase in DNA damage and levels of ROS production. In conclusion, we have developed a novel in vivo model based on culture of cryopreserved sponge cells that is compatible with the alkaline comet assay. Genotoxicity in marine sponges measured by the comet assay technique may be a useful tool for biomonitoring research and risk assessment in aquatic ecosystems. Environ Toxicol Chem 2017;36:3314-3323. © 2017 SETAC.

journal_name

Environ Toxicol Chem

authors

Akpiri RU,Konya RS,Hodges NJ

doi

10.1002/etc.3907

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

3314-3323

issue

12

eissn

0730-7268

issn

1552-8618

journal_volume

36

pub_type

杂志文章
  • Aerobic degradation of ethyl-tert-butyl ether by a microbial consortium: selection and evaluation of biodegradation ability.

    abstract::A microbial consortium that degrades ethyl-tert-butyl ether (ETBE) as the sole source of carbon and energy under aerobic conditions was selected from a gasoline-polluted soil. This consortium consists of a variety of microorganisms with a predominance of filamentous morphology. Degradation of ETBE was found to be sole...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Kharoune M,Kharoune L,Lebault JM,Pauss A

    更新日期:2002-10-01 00:00:00

  • Toxic effects of dietary methylmercury on immune system development in nestling American kestrels (Falco sparverius).

    abstract::This study evaluated the effects of dietary methylmercury (MeHg) on immune system development in captive-reared nestling American kestrels (Falco sparverius) to determine whether T cell-mediated and antibody-mediated adaptive immunity are targets for MeHg toxicity at environmentally relevant concentrations. Nestlings ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.519

    authors: Fallacara DM,Halbrook RS,French JB

    更新日期:2011-06-01 00:00:00

  • Characterization of the estrogenicity of Swiss midland rivers using a recombinant yeast bioassay and plasma vitellogenin concentrations in feral male brown trout.

    abstract::In our study, we aim to characterize the estrogenicity of 18 independent rivers that receive effluent from sewage treatment works. During the winter and summer of 2003, we collected multiple water samples and measured environmental estrogens with an in vitro yeast-based reporter gene assay; estrogenicity was expressed...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/04-305r.1

    authors: Vermeirssen EL,Burki R,Joris C,Peter A,Segner H,Suter MJ,Burkhardt-Holm P

    更新日期:2005-09-01 00:00:00

  • Joint algal toxicity of phenylurea herbicides is equally predictable by concentration addition and independent action.

    abstract::Photosynthesis-inhibiting phenylurea derivatives, such as diuron, are widely used as herbicides. Diuron concentrations clearly exceeding the predicted-no-effect concentration have been regularly measured in European freshwater systems. The frequently observed exposure to mixtures of phenylureas additionally increases ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/02-497

    authors: Backhaus T,Faust M,Scholze M,Gramatica P,Vighi M,Grimme LH

    更新日期:2004-02-01 00:00:00

  • Subchronic exposure of honeybees to sublethal doses of pesticides: effects on behavior.

    abstract::Laboratory bioassays were conducted to evaluate the effects on honeybee behavior of sublethal doses of insecticides chronically administered orally or by contact. Emergent honeybees received a daily dose of insecticide ranging from one-fifth to one-five-hundredth of the median lethal dose (LD50) during 11 d. After exp...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/08-110.1

    authors: Aliouane Y,El Hassani AK,Gary V,Armengaud C,Lambin M,Gauthier M

    更新日期:2009-01-01 00:00:00

  • Protectiveness of Cu water quality criteria against impairment of behavior and chemo/mechanosensory responses: An update.

    abstract::A meta-analysis was conducted of studies that reported behavior and chemo/mechanosensory responses by fish, amphibians, and aquatic invertebrates in Cu-containing waters and also reported sufficient water chemistry for calculation of hardness-based and biotic ligand model (BLM)-based water quality criteria (WQC) for C...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章,评审

    doi:10.1002/etc.4096

    authors: Meyer JS,DeForest DK

    更新日期:2018-05-01 00:00:00

  • Electron paramagnetic resonance investigation of in vivo free radical formation and oxidative stress induced by 2,4-dichlorophenol in the freshwater fish Carassius auratus.

    abstract::In the present study, electron paramagnetic resonance coupled with spin-trapping technique was used, with alpha-phenyl-N-tert-butylnitrone (PBN) as a spin-trapping agent, to investigate free radical generation in freshwater fish with acute 2,4-dichlorophenol (2,4-DCP) poisoning. The PBN-radical adducts were detected i...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/04-640r.1

    authors: Luo Y,Wang XR,Shi HH,Mao DQ,Sui YX,Ji LL

    更新日期:2005-09-01 00:00:00

  • Responses of microbial community from northern Gulf of Mexico sandy sediments following exposure to Deepwater Horizon crude oil.

    abstract::In the present study, microbial community responses to exposure to unweathered Macondo Well crude oil and conventional diesel in a sandy beach environment were determined. Biodegradation was assessed in mesocosm experiments with differing fuel amounts (2,000 and 4,000 mg/kg) and with or without inorganic nutrient amen...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.1770

    authors: Horel A,Mortazavi B,Sobecky PA

    更新日期:2012-05-01 00:00:00

  • Lethal and behavioral impacts of diesel and fuel oil on the Antarctic amphipod Paramoera walkeri.

    abstract::Toxicity testing with Antarctic species is required for risk assessment of fuel spills in Antarctic coastal waters. The lethal and sublethal (movement behavior) sensitivities of adults and juveniles of the Antarctic amphipod Paramoera walkeri to the water accommodated fractions (WAFs) of 3 fuels were estimated in exte...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3778

    authors: Brown KE,King CK,Harrison PL

    更新日期:2017-09-01 00:00:00

  • Use of the Australian crimson-spotted rainbowfish (Melanotaenia fluviatilis) as a model test species for investigating the effects of endocrine disruptors.

    abstract::Few studies have investigated the potential reproductive effects of toxicants on Australian freshwater fish species. The present study uses the Australian rainbowfish (Melanotaenia fluviatilis) as a model for testing the potential effects of 17beta-estradiol. Groups of reproductively active rainbowfish were exposed to...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/06-603R.1

    authors: Pollino CA,Georgiades E,Holdway DA

    更新日期:2007-10-01 00:00:00

  • Seasonality effects on pharmaceuticals and s-triazine herbicides in wastewater effluent and surface water from the Canadian side of the upper Detroit River.

    abstract::The influence of seasonal changes in water conditions and parameters on several major pharmacologically active compounds (PhACs) and s-triazine herbicides was assessed in the wastewater and sewage treatment plant (WSTP) effluent as well as the downstream surface water from sites on the Canadian side of the upper Detro...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/05-571r.1

    authors: Hua WY,Bennett ER,Maio XS,Metcalfe CD,Letcher RJ

    更新日期:2006-09-01 00:00:00

  • Gene expression in caged fish as a first-tier indicator of contaminant exposure in streams.

    abstract::The development of sensitive, biologically based indicators of contaminant exposure (i.e., biomarkers) is an ongoing topic of research. These indicators have been proposed as a first-tier method of identifying contaminant exposure. The primary objective of this research was to implement a biomarker-based method of exp...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/05-137r.1

    authors: Roberts AP,Oris JT,Burton GA Jr,Clements WH

    更新日期:2005-12-01 00:00:00

  • Phase distribution of synthetic pyrethroids in runoff and stream water.

    abstract::Synthetic pyrethroids (SPs) are a group of hydrophobic compounds with significant aquatic toxicity. Their strong affinity to suspended solids and humic materials suggests that SPs in natural surface water are distributed in solid-adsorbed, dissolved organic matter (DOM)-adsorbed, and freely dissolved phases. The freel...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/03-183

    authors: Liu W,Gan JJ,Lee S,Kabashima JN

    更新日期:2004-01-01 00:00:00

  • Estimating the combined toxicity by two-step prediction model on the complicated chemical mixtures from wastewater treatment plant effluents.

    abstract::The toxicities of chemical mixtures containing 10 compounds, detected in wastewater treatment plant (WWTP) effluents, were investigated using Daphnia magna in a two-step prediction (TSP) model. The 10 chemicals determined by gas chromatography/ mass spectrometry in WWTP effluents included three groups: Three acetylcho...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/05-484r.1

    authors: Ra JS,Lee BC,Chang NI,Kim SD

    更新日期:2006-08-01 00:00:00

  • Lead distributions and risks in New Orleans following Hurricanes Katrina and Rita.

    abstract::During the last four years, significant effort has been devoted to understanding the effects that Hurricanes Katrina and Rita had on contaminant distribution and redistribution in New Orleans, Louisiana, USA, and the surrounding Gulf Coast area. Elevated concentrations were found for inorganic contaminants (including ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.205

    authors: Abel MT,Cobb GP,Presley SM,Ray GL,Rainwater TR,Austin GP,Cox SB,Anderson TA,Leftwich BD,Kendall RJ,Suedel BC

    更新日期:2010-07-01 00:00:00

  • Photosynthetic and cellular toxicity of cadmium in Chlorella vulgaris.

    abstract::The toxic effects of cadmium (Cd) on the green alga Chlorella vulgaris were investigated by following the response to Cd of various toxicity endpoints (cell growth, cell size, photochemical efficiency of PSII in the light or Φ(PSII), maximal photochemical efficiency or Fv/Fm, chlorophyll a fluorescence, esterase activ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.2362

    authors: Ou-Yang HL,Kong XZ,Lavoie M,He W,Qin N,He QS,Yang B,Wang R,Xu FL

    更新日期:2013-12-01 00:00:00

  • Factors affecting the occurrence and enantiomeric degradation of hexachlorocyclohexane isomers in northern and temperate aquatic systems.

    abstract::Concentrations of (alpha- and gamma-hexachlorocyclohexane (HCH), alpha/gamma-HCH ratios, and enantiomer ratios (ER) of alpha-HCH were measured in lakes in the arctic, subarctic, Great Lakes, Canada, and temperate regions, and temperate and arctic wetlands and streams. The highest concentrations of alpha-HCH were found...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Law SA,Diamond ML,Helm PA,Jantunen LM,Alaee M

    更新日期:2001-12-01 00:00:00

  • Thiamethoxam honey bee colony feeding study: Linking effects at the level of the individual to those at the colony level.

    abstract::Neonicotinoid insecticides have been used globally on a wide range of crops through seed treatment as well as soil and foliar applications and have been increasingly studied in relation to the potential risk to bees because of their detection in pollen and nectar of bee-attractive crops. The present article reports th...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.4018

    authors: Overmyer J,Feken M,Ruddle N,Bocksch S,Hill M,Thompson H

    更新日期:2018-03-01 00:00:00

  • Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus, L.

    abstract::Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (Leuciscus cephalus), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hy...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:

    authors: Machala M,Dusek L,Hilscherová K,Kubínová R,Jurajda P,Neca J,Ulrich R,Gelnar M,Studnicková Z,Holoubek I

    更新日期:2001-05-01 00:00:00

  • Heavy metal-induced toxicity in the Indian green frog: Biochemical and histopathological alterations.

    abstract::Heavy metal contamination may have adverse effects on wetland biota, particularly on amphibians. Severe immunotoxic effects elicited in Euphlyctis hexadactylus (Indian green frog) because of metal exposure (Cd, Cr, Cu, Pb, and Zn) in the Bellanwila-Attidiya Sanctuary, a polluted urban wetland in Sri Lanka, provided th...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3848

    authors: Jayawardena UA,Angunawela P,Wickramasinghe DD,Ratnasooriya WD,Udagama PV

    更新日期:2017-10-01 00:00:00

  • Creation of a Curated Aquatic Toxicology Database: EnviroTox.

    abstract::Flexible, rapid, and predictive approaches that do not require the use of large numbers of vertebrate test animals are needed because the chemical universe remains largely untested for potential hazards. Development of robust new approach methodologies and nontesting approaches requires the use of existing information...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.4382

    authors: Connors KA,Beasley A,Barron MG,Belanger SE,Bonnell M,Brill JL,de Zwart D,Kienzler A,Krailler J,Otter R,Phillips JL,Embry MR

    更新日期:2019-05-01 00:00:00

  • Individual and mixture effects of caffeine and sulfamethoxazole on the daggerblade grass shrimp Palaemonetes pugio following maternal exposure.

    abstract::Pharmaceuticals and personal care products (PPCPs) such as caffeine and sulfamethoxazole have been detected in the estuarine environment. The present study characterized effects of a maternal exposure of these compounds on the development of the daggerblade grass shrimp Palaemonetes pugio from embryo to juvenile life ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.2669

    authors: Garcia RN,Chung KW,DeLorenzo ME,Curran MC

    更新日期:2014-09-01 00:00:00

  • Occurrence of endocrine-disrupting pesticide residues in wetland sediments from Beijing, China.

    abstract::Wetlands are important habitats and spawning grounds to a diverse wildlife population and are particularly vulnerable to adverse effects of endocrine disruptors. Many pesticides possess hormonal activity and have thus been classified as endocrine disruptors. A new analytical method based on ultrasonic-assisted extract...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/07-511.1

    authors: Xue N,Li F,Hou H,Li B

    更新日期:2008-05-01 00:00:00

  • A silviculture application of the glyphosate-based herbicide VisionMAX to wetlands has limited direct effects on amphibian larvae.

    abstract::Herbicides are commonly used in agriculture and silviculture to reduce interspecific competition among plants and thereby enhance crop growth, quality, and volume. Internationally, glyphosate-based herbicides are the most widely used herbicides in both of these sectors. Laboratory and mesocosm studies have demonstrate...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.1956

    authors: Edge CB,Thompson DG,Hao C,Houlahan JE

    更新日期:2012-10-01 00:00:00

  • Influence of dimethoate on acetylcholinesterase activity and locomotor function in terrestrial isopods.

    abstract::Locomotor behavior in terrestrial organisms is crucial for burrowing, avoiding predators, food seeking, migration, and reproduction; therefore, it is a parameter with ecological relevance. Acetylcholinesterase (AChE) is a nervous system enzyme inhibited by several compounds and widely used as an exposure biomarker in ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/04-131r.1

    authors: Engenheiro EL,Hankard PK,Sousa JP,Lemos MF,Weeks JM,Soares AM

    更新日期:2005-03-01 00:00:00

  • Persistent organic pollutants in coastal sediment off South China in relation to the importance of anthropogenic inputs.

    abstract::Surface sediments collected from the coastal region off South China were analyzed for persistent organic pollutants (POPs), including polybrominated diphenyl ethers (PBDEs), polycyclic aromatic hydrocarbons (PAHs), and organochlorine pesticides (OCPs). The concentrations of BDE-209, Σ(12)PBDE, Σ(15)PAH, Σ(7)PAH, and Σ...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.1807

    authors: Liu LY,Wang JZ,Qiu JW,Liang Y,Zeng EY

    更新日期:2012-06-01 00:00:00

  • Transcriptional and cellular effects of benzotriazole UV stabilizers UV-234 and UV-328 in the freshwater invertebrates Chlamydomonas reinhardtii and Daphnia magna.

    abstract::Benzotriazole ultra violet stabilizers (BZT-UVs) are compounds used in many applications and products to prevent photochemical degradation. Despite their widespread presence in aquatic ecosystems and persistence in the environment, there are very limited data on their effects and toxicity, and their modes of action re...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.3908

    authors: Giraudo M,Cottin G,Esperanza M,Gagnon P,Silva AO,Houde M

    更新日期:2017-12-01 00:00:00

  • Vapor pressure of solid polybrominated diphenyl ethers determined via Knudsen effusion method.

    abstract::Polybrominated diphenyl ethers (PBDEs) are flame retardants used in a variety of consumer products. The solid vapor pressures of BDE 15 and BDE 209 were determined by using the Knudsen effusion method, and the values measured extrapolated to 298.15 K are 3.12 × 10(-3) and 9.02 × 10(-13) Pa, respectively. The enthalpie...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.621

    authors: Fu J,Suuberg EM

    更新日期:2011-10-01 00:00:00

  • Laboratory culture and life-cycle experiments with the benthic amphipod Melita plumulosa (Zeidler).

    abstract::In Australia, the collection of estuarine invertebrates from the field for whole-sediment toxicity tests is hindered because of temporal variability in their population densities and distributions. The present study aimed to develop culturing procedures for Melita plumulosa (Zeidler), an epibenthic and intertidal, dep...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1897/04-409r1.1

    authors: Hyne RV,Gale SA,King CK

    更新日期:2005-08-01 00:00:00

  • Effects of chronic polybrominated diphenyl ether exposure on gonadal development in the northern leopard frog, Rana pipiens.

    abstract::Polybrominated diphenyl ethers (PBDEs) are bioaccumulative, persistent organic pollutants used as flame retardants in consumer goods. Concentrations of PBDEs in North American wildlife have been increasing for decades and been shown to have estrogenic effects on sexual development. No studies, however, have examined t...

    journal_title:Environmental toxicology and chemistry

    pub_type: 杂志文章

    doi:10.1002/etc.1702

    authors: Van Schmidt ND,Cary TL,Ortiz-Santaliestra ME,Karasov WH

    更新日期:2012-02-01 00:00:00