Abstract:
:Sponges are a potential alternative model species to bivalves in pollution biomonitoring and environmental risk assessment in the aquatic ecosystem. In the present study, a novel in vivo exposure sponge culture model was developed from field-collected and cryopreserved sponge (Hymeniacidon perleve) cells to investigate the genotoxic effects of environmentally relevant metals in the laboratory. Sponge cell aggregates were cultured and exposed to noncytotoxic concentrations (0-0.4 mg/L) of cadmium chloride, nickel chloride, and sodium dichromate as quantified by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and DNA-strand breaks assessed by the comet assay. Reactive oxygen species (ROS) formation was quantified by oxidation of 2',7'-dichlorofluorescin diacetate in sponge cell aggregates exposed to the same concentrations of Cd, Cr, and Ni. There was a statistically significant (p < 0.05) concentration-dependent increase in the level of DNA-strand breaks and ROS formation in all of the metals investigated. To the best of our knowledge, we have utilized for the first time the alkaline comet assay to detect DNA-strand breaks in marine sponge cells and demonstrated that exposure to noncytotoxic concentrations of Cd, Cr, and Ni for 12 h results in a concentration-dependent increase in DNA damage and levels of ROS production. In conclusion, we have developed a novel in vivo model based on culture of cryopreserved sponge cells that is compatible with the alkaline comet assay. Genotoxicity in marine sponges measured by the comet assay technique may be a useful tool for biomonitoring research and risk assessment in aquatic ecosystems. Environ Toxicol Chem 2017;36:3314-3323. © 2017 SETAC.
journal_name
Environ Toxicol Chemjournal_title
Environmental toxicology and chemistryauthors
Akpiri RU,Konya RS,Hodges NJdoi
10.1002/etc.3907subject
Has Abstractpub_date
2017-12-01 00:00:00pages
3314-3323issue
12eissn
0730-7268issn
1552-8618journal_volume
36pub_type
杂志文章abstract::A microbial consortium that degrades ethyl-tert-butyl ether (ETBE) as the sole source of carbon and energy under aerobic conditions was selected from a gasoline-polluted soil. This consortium consists of a variety of microorganisms with a predominance of filamentous morphology. Degradation of ETBE was found to be sole...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::This study evaluated the effects of dietary methylmercury (MeHg) on immune system development in captive-reared nestling American kestrels (Falco sparverius) to determine whether T cell-mediated and antibody-mediated adaptive immunity are targets for MeHg toxicity at environmentally relevant concentrations. Nestlings ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.519
更新日期:2011-06-01 00:00:00
abstract::In our study, we aim to characterize the estrogenicity of 18 independent rivers that receive effluent from sewage treatment works. During the winter and summer of 2003, we collected multiple water samples and measured environmental estrogens with an in vitro yeast-based reporter gene assay; estrogenicity was expressed...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/04-305r.1
更新日期:2005-09-01 00:00:00
abstract::Photosynthesis-inhibiting phenylurea derivatives, such as diuron, are widely used as herbicides. Diuron concentrations clearly exceeding the predicted-no-effect concentration have been regularly measured in European freshwater systems. The frequently observed exposure to mixtures of phenylureas additionally increases ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/02-497
更新日期:2004-02-01 00:00:00
abstract::Laboratory bioassays were conducted to evaluate the effects on honeybee behavior of sublethal doses of insecticides chronically administered orally or by contact. Emergent honeybees received a daily dose of insecticide ranging from one-fifth to one-five-hundredth of the median lethal dose (LD50) during 11 d. After exp...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/08-110.1
更新日期:2009-01-01 00:00:00
abstract::A meta-analysis was conducted of studies that reported behavior and chemo/mechanosensory responses by fish, amphibians, and aquatic invertebrates in Cu-containing waters and also reported sufficient water chemistry for calculation of hardness-based and biotic ligand model (BLM)-based water quality criteria (WQC) for C...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章,评审
doi:10.1002/etc.4096
更新日期:2018-05-01 00:00:00
abstract::In the present study, electron paramagnetic resonance coupled with spin-trapping technique was used, with alpha-phenyl-N-tert-butylnitrone (PBN) as a spin-trapping agent, to investigate free radical generation in freshwater fish with acute 2,4-dichlorophenol (2,4-DCP) poisoning. The PBN-radical adducts were detected i...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/04-640r.1
更新日期:2005-09-01 00:00:00
abstract::In the present study, microbial community responses to exposure to unweathered Macondo Well crude oil and conventional diesel in a sandy beach environment were determined. Biodegradation was assessed in mesocosm experiments with differing fuel amounts (2,000 and 4,000 mg/kg) and with or without inorganic nutrient amen...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1770
更新日期:2012-05-01 00:00:00
abstract::Toxicity testing with Antarctic species is required for risk assessment of fuel spills in Antarctic coastal waters. The lethal and sublethal (movement behavior) sensitivities of adults and juveniles of the Antarctic amphipod Paramoera walkeri to the water accommodated fractions (WAFs) of 3 fuels were estimated in exte...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.3778
更新日期:2017-09-01 00:00:00
abstract::Few studies have investigated the potential reproductive effects of toxicants on Australian freshwater fish species. The present study uses the Australian rainbowfish (Melanotaenia fluviatilis) as a model for testing the potential effects of 17beta-estradiol. Groups of reproductively active rainbowfish were exposed to...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/06-603R.1
更新日期:2007-10-01 00:00:00
abstract::The influence of seasonal changes in water conditions and parameters on several major pharmacologically active compounds (PhACs) and s-triazine herbicides was assessed in the wastewater and sewage treatment plant (WSTP) effluent as well as the downstream surface water from sites on the Canadian side of the upper Detro...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-571r.1
更新日期:2006-09-01 00:00:00
abstract::The development of sensitive, biologically based indicators of contaminant exposure (i.e., biomarkers) is an ongoing topic of research. These indicators have been proposed as a first-tier method of identifying contaminant exposure. The primary objective of this research was to implement a biomarker-based method of exp...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-137r.1
更新日期:2005-12-01 00:00:00
abstract::Synthetic pyrethroids (SPs) are a group of hydrophobic compounds with significant aquatic toxicity. Their strong affinity to suspended solids and humic materials suggests that SPs in natural surface water are distributed in solid-adsorbed, dissolved organic matter (DOM)-adsorbed, and freely dissolved phases. The freel...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/03-183
更新日期:2004-01-01 00:00:00
abstract::The toxicities of chemical mixtures containing 10 compounds, detected in wastewater treatment plant (WWTP) effluents, were investigated using Daphnia magna in a two-step prediction (TSP) model. The 10 chemicals determined by gas chromatography/ mass spectrometry in WWTP effluents included three groups: Three acetylcho...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/05-484r.1
更新日期:2006-08-01 00:00:00
abstract::During the last four years, significant effort has been devoted to understanding the effects that Hurricanes Katrina and Rita had on contaminant distribution and redistribution in New Orleans, Louisiana, USA, and the surrounding Gulf Coast area. Elevated concentrations were found for inorganic contaminants (including ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.205
更新日期:2010-07-01 00:00:00
abstract::The toxic effects of cadmium (Cd) on the green alga Chlorella vulgaris were investigated by following the response to Cd of various toxicity endpoints (cell growth, cell size, photochemical efficiency of PSII in the light or Φ(PSII), maximal photochemical efficiency or Fv/Fm, chlorophyll a fluorescence, esterase activ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.2362
更新日期:2013-12-01 00:00:00
abstract::Concentrations of (alpha- and gamma-hexachlorocyclohexane (HCH), alpha/gamma-HCH ratios, and enantiomer ratios (ER) of alpha-HCH were measured in lakes in the arctic, subarctic, Great Lakes, Canada, and temperate regions, and temperate and arctic wetlands and streams. The highest concentrations of alpha-HCH were found...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::Neonicotinoid insecticides have been used globally on a wide range of crops through seed treatment as well as soil and foliar applications and have been increasingly studied in relation to the potential risk to bees because of their detection in pollen and nectar of bee-attractive crops. The present article reports th...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.4018
更新日期:2018-03-01 00:00:00
abstract::Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (Leuciscus cephalus), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hy...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::Heavy metal contamination may have adverse effects on wetland biota, particularly on amphibians. Severe immunotoxic effects elicited in Euphlyctis hexadactylus (Indian green frog) because of metal exposure (Cd, Cr, Cu, Pb, and Zn) in the Bellanwila-Attidiya Sanctuary, a polluted urban wetland in Sri Lanka, provided th...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.3848
更新日期:2017-10-01 00:00:00
abstract::Flexible, rapid, and predictive approaches that do not require the use of large numbers of vertebrate test animals are needed because the chemical universe remains largely untested for potential hazards. Development of robust new approach methodologies and nontesting approaches requires the use of existing information...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.4382
更新日期:2019-05-01 00:00:00
abstract::Pharmaceuticals and personal care products (PPCPs) such as caffeine and sulfamethoxazole have been detected in the estuarine environment. The present study characterized effects of a maternal exposure of these compounds on the development of the daggerblade grass shrimp Palaemonetes pugio from embryo to juvenile life ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.2669
更新日期:2014-09-01 00:00:00
abstract::Wetlands are important habitats and spawning grounds to a diverse wildlife population and are particularly vulnerable to adverse effects of endocrine disruptors. Many pesticides possess hormonal activity and have thus been classified as endocrine disruptors. A new analytical method based on ultrasonic-assisted extract...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/07-511.1
更新日期:2008-05-01 00:00:00
abstract::Herbicides are commonly used in agriculture and silviculture to reduce interspecific competition among plants and thereby enhance crop growth, quality, and volume. Internationally, glyphosate-based herbicides are the most widely used herbicides in both of these sectors. Laboratory and mesocosm studies have demonstrate...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1956
更新日期:2012-10-01 00:00:00
abstract::Locomotor behavior in terrestrial organisms is crucial for burrowing, avoiding predators, food seeking, migration, and reproduction; therefore, it is a parameter with ecological relevance. Acetylcholinesterase (AChE) is a nervous system enzyme inhibited by several compounds and widely used as an exposure biomarker in ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/04-131r.1
更新日期:2005-03-01 00:00:00
abstract::Surface sediments collected from the coastal region off South China were analyzed for persistent organic pollutants (POPs), including polybrominated diphenyl ethers (PBDEs), polycyclic aromatic hydrocarbons (PAHs), and organochlorine pesticides (OCPs). The concentrations of BDE-209, Σ(12)PBDE, Σ(15)PAH, Σ(7)PAH, and Σ...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1807
更新日期:2012-06-01 00:00:00
abstract::Benzotriazole ultra violet stabilizers (BZT-UVs) are compounds used in many applications and products to prevent photochemical degradation. Despite their widespread presence in aquatic ecosystems and persistence in the environment, there are very limited data on their effects and toxicity, and their modes of action re...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.3908
更新日期:2017-12-01 00:00:00
abstract::Polybrominated diphenyl ethers (PBDEs) are flame retardants used in a variety of consumer products. The solid vapor pressures of BDE 15 and BDE 209 were determined by using the Knudsen effusion method, and the values measured extrapolated to 298.15 K are 3.12 × 10(-3) and 9.02 × 10(-13) Pa, respectively. The enthalpie...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.621
更新日期:2011-10-01 00:00:00
abstract::In Australia, the collection of estuarine invertebrates from the field for whole-sediment toxicity tests is hindered because of temporal variability in their population densities and distributions. The present study aimed to develop culturing procedures for Melita plumulosa (Zeidler), an epibenthic and intertidal, dep...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1897/04-409r1.1
更新日期:2005-08-01 00:00:00
abstract::Polybrominated diphenyl ethers (PBDEs) are bioaccumulative, persistent organic pollutants used as flame retardants in consumer goods. Concentrations of PBDEs in North American wildlife have been increasing for decades and been shown to have estrogenic effects on sexual development. No studies, however, have examined t...
journal_title:Environmental toxicology and chemistry
pub_type: 杂志文章
doi:10.1002/etc.1702
更新日期:2012-02-01 00:00:00