Abstract：
:We conducted quantitative cellular respiration analysis on samples taken from human breast cancer (HBC) and human colorectal cancer (HCC) patients. Respiratory capacity is not lost as a result of tumor formation and even though, functionally, complex I in HCC was found to be suppressed, it was not evident on the protein level. Additionally, metabolic control analysis was used to quantify the role of components of mitochondrial interactosome. The main rate-controlling steps in HBC are complex IV and adenine nucleotide transporter, but in HCC, complexes I and III. Our kinetic measurements confirmed previous studies that respiratory chain complexes I and III in HBC and HCC can be assembled into supercomplexes with a possible partial addition from the complex IV pool. Therefore, the kinetic method can be a useful addition in studying supercomplexes in cell lines or human samples. In addition, when results from culture cells were compared to those from clinical samples, clear differences were present, but we also detected two different types of mitochondria within clinical HBC samples, possibly linked to two-compartment metabolism. Taken together, our data show that mitochondrial respiration and regulation of mitochondrial membrane permeability have substantial differences between these two cancer types when compared to each other to their adjacent healthy tissue or to respective cell cultures.
journal_name
Oxid Med Cell Longevjournal_title
Oxidative medicine and cellular longevityauthors
Koit A,Shevchuk I,Ounpuu L,Klepinin A,Chekulayev V,Timohhina N,Tepp K,Puurand M,Truu L,Heck K,Valvere V,Guzun R,Kaambre Tdoi
10.1155/2017/1372640subject
Has Abstractpub_date
2017-01-01 00:00:00pages
1372640eissn
1942-0900issn
1942-0994journal_volume
2017pub_type
杂志文章abstract：:Diabetes is a disease that can be treated with oral antidiabetic agents and/or insulin. However, patients' metabolic control is inadequate in a high percentage of them and a major cause of chronic diseases like diabetic retinopathy. Approximately 15% of patients have some degree of diabetic retinopathy when diabetes i...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2019/4940825
更新日期：2019-11-11 00:00:00
abstract：:Obesity is a state of chronic low-level inflammation closely associated with oxidative stress. Childhood obesity is associated with endothelial dysfunction, inflammation, and oxidative stress markers individually. This study was aimed at determining the association between the biomarkers of inflammation, oxidative str...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/9604740
更新日期：2019-10-16 00:00:00
abstract：Background:Obesity is a growing epidemic with limited effective treatments and an important risk factor for several diseases such as metabolic syndrome (MetS). In this study, we aimed to investigate the effect of 3-amino-1,2,4-triazole (ATZ), an inhibitor of catalase and heme synthesis, in a murine model for MetS. Met...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/3025361
更新日期：2020-04-13 00:00:00
abstract：:Aluminum ions are especially toxic to plants in acidic soils. Here we present evidences that SO2 protects germinating wheat grains against aluminum stress. SO2 donor (NaHSO3/Na2SO3) pretreatment at 1.2 mM reduced the accumulation of superoxide anion, hydrogen peroxide, and malondialdehyde, enhanced the activities of g...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/612363
更新日期：2015-01-01 00:00:00
abstract：:Serine-threonine kinase receptor-associated protein (STRAP) is a transforming growth factor β (TGF-β) receptor-interacting protein that has been implicated in both cell proliferation and cell death in response to various stresses. However, the precise roles of STRAP in these cellular processes are still unclear. The m...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2018/5241524
更新日期：2018-04-19 00:00:00
abstract：:Curcumin has anti-inflammatory, antioxidative, anticarcinogenic, and cardiovascular protective effects. Our study is aimed at evaluating the effects of pretreatment with curcumin nanoparticles (CCNP) compared to conventional curcumin (CC) on isoproterenol (ISO) induced myocardial infarction (MI) in rats. Fifty-six Wis...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/7847142
更新日期：2019-05-07 00:00:00
abstract：:Recent reports indicate that oxidative stress is involved in the pathobiology of acute spinal cord injury or compression myelopathy. We conducted an observational study to determine levels of oxidative stress markers in serum from 80 patients who underwent spinal surgery to treat neurological symptoms related to lumba...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2020/5649767
更新日期：2020-12-24 00:00:00
abstract：:Organophosphate pesticides (OPs) are among the most widely used synthetic chemicals for the control of a wide variety of pests, and reactive oxygen species (ROS) caused by OPs may be involved in the toxicity of various pesticides. Previous studies have demonstrated that a reactivation of latent Epstein-Barr virus (EBV...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/309125
更新日期：2015-01-01 00:00:00
abstract：:Doxorubicin (DOX) is an efficient chemotherapeutic agent, but its clinical application is limited by its cardiotoxicity associated with increased oxidative stress. Thus, the combination of DOX and antioxidants has been encouraged. In this study, we evaluated (I) the chemical composition and antioxidant capacity of aqu...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/2935051
更新日期：2018-06-28 00:00:00
abstract：Introduction:The omega-3 polyunsaturated fatty acids, as docosahexaenoic acid (DHA), are considered mediators regulating the resolution of inflammation during cancer and may be associated with better outcomes. Epoxydocosapentaenoic acids (EDPs), metabolites of the DHA, are hypothesized to be responsible for some benefi...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/1280987
更新日期：2019-03-05 00:00:00
abstract：:Postoperative cognitive dysfunction (POCD) is a common sequela following surgery and hospitalization. The prevention and management of POCD are important during clinical practice. POCD more commonly affects elderly patients who have undergone major surgery and can result in major decline in quality of life for both pa...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2020/9641904
更新日期：2020-02-13 00:00:00
abstract：:2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG) was isolated from Polygonum multiflorum, a plant which is traditionally used as an anti-ageing drug. We have analysed ageing-related effects of TSG in the model organism C. elegans in comparison to resveratrol. TSG exerted a high antioxidative capacity both in a ce...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/124357
更新日期：2015-01-01 00:00:00
abstract：:Oxidative stress plays a crucial role in the pathogenesis of acute pancreatitis (AP). Isoliquiritigenin (ISL) is a flavonoid monomer with confirmed antioxidant activity. However, the specific effects of ISL on AP have not been determined. In this study, we aimed to investigate the protective effect of ISL on AP using ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/7161592
更新日期：2018-04-26 00:00:00
abstract：:Hippocampal neurogenesis is changed by brain injury. When neuroinflammation accompanies injury, activation of resident microglial cells promotes the release of inflammatory cytokines and reactive oxygen/nitrogen species like nitric oxide (NO). In these conditions, NO promotes proliferation of neural stem cells (NSC) i...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2015/451512
更新日期：2015-01-01 00:00:00
abstract：Purpose:Oxidative stress induced by reduced blood circulation is a critical pathological damage to retinal ganglion cells (RGCs) in glaucoma. We previously showed that green tea extract (GTE) and its catechin constituents alleviate sodium iodate-induced retinal degeneration in rats. Here, we investigated the therapeuti...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/8407206
更新日期：2019-07-09 00:00:00
abstract：:Metabolic disorders are severe and chronic impairments of the health of many people and represent a challenge for the society as a whole that has to deal with an ever-increasing number of affected individuals. Among common metabolic disorders are Alzheimer's disease, obesity, and type 2 diabetes. These disorders do no...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2019/7210892
更新日期：2019-06-16 00:00:00
abstract：:Hepatocellular carcinoma (HCC) accounts for over 80% of liver cancer cases and is highly malignant, recurrent, drug-resistant, and often diagnosed in the advanced stage. It is clear that early diagnosis and a better understanding of molecular mechanisms contributing to HCC progression is clinically urgent. Metabolic a...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2018/7512159
更新日期：2018-11-04 00:00:00
abstract：:The role of altered redox status and high reactive oxygen species (ROS) is still controversial in cancer development and progression. Intracellular levels of ROS are elevated in cancer cells suggesting a role in cancer initiation and progression; on the contrary, ROS elevated levels may induce programmed cell death an...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2017/2597581
更新日期：2017-01-01 00:00:00
abstract：:As the involvement of oxidative stress (OS) in autoimmune thrombocytopenia (AITP) has been reported, a fast and rapid test for the reliable measurement of OS and antioxidant capacities (AOCs) might be a useful tool in extending current diagnostic possibilities. The free oxygen radical test (FORT) and free oxygen radic...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2014/720347
更新日期：2014-01-01 00:00:00
abstract：:A disturbed homeostasis of cellular lipids and the resulting lipotoxicity are considered to be key contributors to many human pathologies, including obesity, metabolic syndrome, type 2 diabetes, cardiovascular diseases, and cancer. The yeast Saccharomyces cerevisiae has been successfully used for uncovering molecular ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章,评审
doi：10.1155/2018/3074769
更新日期：2018-01-31 00:00:00
abstract：:As one of the major risks for urolithiasis, hyperoxaluria can be caused by genetic defect or dietary intake. And high oxalate induced renal epithelial cells injury is related to oxidative stress and mitochondrial dysfunction. Here, we investigated whether MitoTEMPO, a mitochondria-targeted antioxidant, could protect a...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2017/7528090
更新日期：2017-01-01 00:00:00
abstract：:Ascorbate requiring Fe2+/2-oxoglutarate-dependent dioxygenases located in the nucleoplasm have been shown to participate in epigenetic regulation of gene expression via histone and DNA demethylation. Transport of dehydroascorbic acid is impaired in the endomembranes of fibroblasts from arterial tortuosity syndrome (AT...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/8156592
更新日期：2019-01-13 00:00:00
abstract：:Purpose. To investigate the preventive effect of resveratrol (RES) on the formation of portal vein system thrombosis (PVST) in a rat fibrosis model. Methods. A total of 64 male SD rats, weighing 200-300 g, were divided into five groups: Sham operation, Splenectomy I, Splenectomy II, RES, and low molecular weight hepar...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/7453849
更新日期：2016-01-01 00:00:00
abstract：:Three cosmetically important skin lightening agents, hydroquinone (HQ), kojic acid (KA), and niacinamide (NA), consume the bulk of successful skin lightening ingredients in cosmetic applications. However, the mechanisms by which these ingredients work are still unclear. In this study, melanocytes and keratinocytes wer...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2013/702120
更新日期：2013-01-01 00:00:00
abstract：:We investigated the effect of high-frequency electromagnetic fields (HF-EMFs) and 17-β-estradiol on connexins (Cxs), integrins (Ints), and estrogen receptor (ER) expression, as well as on ultrastructure of trophoblast-derived HTR-8/SVneo cells. HF-EMF, 17-β-estradiol, and their combination induced an increase of Cx40 ...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2013/280850
更新日期：2013-01-01 00:00:00
abstract：:Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery tightened for 6 h the...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2018/6012636
更新日期：2018-04-02 00:00:00
abstract：Objective:Cholesterol oxidation products have an established proatherogenic and cytotoxic effect. An increased exposure to these substances may be associated with the development of atherosclerosis and cancers. Relatively little, though, is known about the effect of phytosterol oxidation products, although phytosterols...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2019/1983975
更新日期：2019-11-15 00:00:00
abstract：:Fruits, such as grapes, are essential food of the Mediterranean diet. Grape extracts have potent antioxidant and chemopreventive properties in vitro. Numerous studies have examined the effects of plant extract administration on redox status at rest in animals and humans but their results are controversial. However, th...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2012/185867
更新日期：2012-01-01 00:00:00
abstract：:Oxidative stress plays an important role in the pathogenesis of endothelial dysfunction, which is found to precede the development of diverse cardiovascular diseases (CVDs). The aim of this study was to observe the protective effects of PD against H2O2-induced oxidative stress injury (OSI) in human umbilical vein endo...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/5139458
更新日期：2016-01-01 00:00:00
abstract：:The vascular endothelium plays an essential role in the control of the blood flow. Pharmacological agents like quinone (menadione) at various doses modulate this process in a variety of ways. In this study, Q7, a 2-phenylamino-1,4-naphthoquinone derivative, significantly increased oxidative stress and induced vascular...
journal_title：Oxidative medicine and cellular longevity
pub_type： 杂志文章
doi：10.1155/2016/3939540
更新日期：2016-01-01 00:00:00
