Low-Dose Ionizing Radiation Affects Mesenchymal Stem Cells via Extracellular Oxidized Cell-Free DNA: A Possible Mediator of Bystander Effect and Adaptive Response.

Abstract:

:We have hypothesized that the adaptive response to low doses of ionizing radiation (IR) is mediated by oxidized cell-free DNA (cfDNA) fragments. Here, we summarize our experimental evidence for this model. Studies involving measurements of ROS, expression of the NOX (superoxide radical production), induction of apoptosis and DNA double-strand breaks, antiapoptotic gene expression and cell cycle inhibition confirm this hypothesis. We have demonstrated that treatment of mesenchymal stem cells (MSCs) with low doses of IR (10 cGy) leads to cell death of part of cell population and release of oxidized cfDNA. cfDNA has the ability to penetrate into the cytoplasm of other cells. Oxidized cfDNA, like low doses of IR, induces oxidative stress, ROS production, ROS-induced oxidative modifications of nuclear DNA, DNA breaks, arrest of the cell cycle, activation of DNA reparation and antioxidant response, and inhibition of apoptosis. The MSCs pretreated with low dose of irradiation or oxidized cfDNA were equally effective in induction of adaptive response to challenge further dose of radiation. Our studies suggest that oxidized cfDNA is a signaling molecule in the stress signaling that mediates radiation-induced bystander effects and that it is an important component of the development of radioadaptive responses to low doses of IR.

journal_name

Oxid Med Cell Longev

authors

Sergeeva VA,Ershova ES,Veiko NN,Malinovskaya EM,Kalyanov AA,Kameneva LV,Stukalov SV,Dolgikh OA,Konkova MS,Ermakov AV,Veiko VP,Izhevskaya VL,Kutsev SI,Kostyuk SV

doi

10.1155/2017/9515809

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

9515809

eissn

1942-0900

issn

1942-0994

journal_volume

2017

pub_type

杂志文章
  • Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep Monocytes/Macrophages.

    abstract::Many groups of Gram-negative bacteria cause diseases that are harmful to sheep. Toll-like receptor 4 (TLR4), which is critical for detecting Gram-negative bacteria by the innate immune system, is activated by lipopolysaccharide (LPS) to initiate inflammatory responses and oxidative stress. Oxidation intermediates are ...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2015/359315

    authors: Deng S,Yu K,Zhang B,Yao Y,Wang Z,Zhang J,Zhang X,Liu G,Li N,Liu Y,Lian Z

    更新日期:2015-01-01 00:00:00

  • Ginkgolide B Suppresses TLR4-Mediated Inflammatory Response by Inhibiting the Phosphorylation of JAK2/STAT3 and p38 MAPK in High Glucose-Treated HUVECs.

    abstract:AIM:Ginkgolide B is a Ginkgo biloba leaf extract that has been identified as a natural platelet-activating factor receptor (PAFR) antagonist. We investigated the effect of ginkgolide B on high glucose-induced TLR4 activation in human umbilical vein endothelial cells (HUVECs). METHODS:Protein expression was analyzed by...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2017/9371602

    authors: Chen K,Sun W,Jiang Y,Chen B,Zhao Y,Sun J,Gong H,Qi R

    更新日期:2017-01-01 00:00:00

  • Simvastatin Ameliorates Diabetic Cardiomyopathy by Attenuating Oxidative Stress and Inflammation in Rats.

    abstract::Simvastatin is a lipid-lowering agent used to treat hypercholesterolemia and to reduce the risk of heart disease. This study scrutinized the beneficial effects of simvastatin on experimental diabetic cardiomyopathy (DCM), pointing to the role of hyperglycemia-induced oxidative stress and inflammation. Diabetes was ind...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2017/1092015

    authors: Al-Rasheed NM,Al-Rasheed NM,Hasan IH,Al-Amin MA,Al-Ajmi HN,Mohamad RA,Mahmoud AM

    更新日期:2017-01-01 00:00:00

  • Recovery of Cardiac Remodeling and Dysmetabolism by Pancreatic Islet Injury Improvement in Diabetic Rats after Yacon Leaf Extract Treatment.

    abstract::Yacon (Smallanthus sonchifolius) is a native Andean plant rich in phenolic compounds, and its effects on dysmetabolism and cardiomyopathy in diabetic rats was evaluated. The rats (10/group) were allocated as follows: C, controls; C + Y, controls treated with Yacon leaf extract (YLE); DM, diabetic controls; and DM + Y,...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2018/1821359

    authors: Dos Santos KC,Cury SS,Ferraz APCR,Corrente JE,Gonçalves BM,de Araújo Machado LH,Carvalho RF,de Melo Stevanato Nakamune AC,Fabro AT,Freire PP,Corrêa CR

    更新日期:2018-04-10 00:00:00

  • Integrated Metabolomic and Lipidomic Analysis Reveals the Neuroprotective Mechanisms of Bushen Tiansui Formula in an Aβ1-42-Induced Rat Model of Alzheimer's Disease.

    abstract::Bushen Tiansui Formula (BSTSF) is a traditional Chinese medicine prescription. It has been widely applied to treat Alzheimer's disease (AD) in the clinic; however, the mechanisms underlying its effects remain largely unknown. In this study, we used a rat AD model to study the effects of BSTSF on cognitive performance,...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2020/5243453

    authors: Yi M,Zhang C,Zhang Z,Yi P,Xu P,Huang J,Peng W

    更新日期:2020-06-19 00:00:00

  • Thiol redox sensitivity of two key enzymes of heme biosynthesis and pentose phosphate pathways: uroporphyrinogen decarboxylase and transketolase.

    abstract::Uroporphyrinogen decarboxylase (Hem12p) and transketolase (Tkl1p) are key mediators of two critical processes within the cell, heme biosynthesis, and the nonoxidative part of the pentose phosphate pathway (PPP). The redox properties of both Hem12p and Tkl1p from Saccharomyces cerevisiae were investigated using proteom...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2013/932472

    authors: McDonagh B,Pedrajas JR,Padilla CA,Bárcena JA

    更新日期:2013-01-01 00:00:00

  • Reactive Oxygen Species from NADPH Oxidase and Mitochondria Participate in the Proliferation of Aortic Smooth Muscle Cells from a Model of Metabolic Syndrome.

    abstract::In metabolic diseases, the increased reactive oxygen species (ROS) represents one of the pathogenic mechanisms for vascular disease probably by promoting vascular smooth muscle cell (SMC) proliferation that contributes to the development of arterial remodeling and stenosis, hypertension, and atherosclerosis. Therefore...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2018/5835072

    authors: López-Acosta O,de Los Angeles Fortis-Barrera M,Barrios-Maya MA,Ramírez AR,Aguilar FJA,El-Hafidi M

    更新日期:2018-12-23 00:00:00

  • Effects of Redox Disturbances on Intestinal Contractile Reactivity in Rats Fed with a Hypercaloric Diet.

    abstract::Few studies have associated the effects of changes in caloric intake and redox disturbances in the gastrointestinal tract. Therefore, the present study aimed at evaluating the hypercaloric diet consumption influence on the contractile reactivity of intestinal smooth muscle, morphology, and oxidative stress of rat ileu...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2018/6364821

    authors: de Souza ILL,Ferreira EDS,Diniz AFA,Carvalho MTL,Queiroga FR,Toscano LT,Silva AS,da Silva PM,Cavalcante FA,da Silva BA

    更新日期:2018-10-25 00:00:00

  • REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit.

    abstract::Hemoglobin (Hb) is a family of proteins in red blood cells responsible for oxygen transport and vulnerable for oxidative damage. Hemoglobin δ subunit (HBD), a member of Hb family, is normally expressed by cells of erythroid lineage. Expression of Hb genes has been previously reported in nonerythroid and hematopoietic ...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2017/7295319

    authors: Zuo Q,Cheng S,Huang W,Bhatti MZ,Xue Y,Zhang Y,Zhang B,Li L,Wu L,Fu J,Chen J,Li X

    更新日期:2017-01-01 00:00:00

  • Distribution of Paraoxonase-1 (PON-1) and Lipoprotein Phospholipase A2 (Lp-PLA2) across Lipoprotein Subclasses in Subjects with Type 2 Diabetes.

    abstract::Paraoxonase-1 (PON1) and lipoprotein phospholipase A2 (Lp-PLA2) may exert an important protective role by preventing the oxidative transformation of high- and low-density lipoproteins (HDL and LDL, respectively). The activity of both enzymes is influenced by lipidome and proteome of the lipoprotein carriers. T2DM typi...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2018/1752940

    authors: Passaro A,Vigna GB,Romani A,Sanz JM,Cavicchio C,Bonaccorsi G,Valacchi G,Cervellati C

    更新日期:2018-11-05 00:00:00

  • Nicotine and Cotinine Inhibit Catalase and Glutathione Reductase Activity Contributing to the Impaired Osteogenesis of SCP-1 Cells Exposed to Cigarette Smoke.

    abstract::Cigarette smoking has been identified as a major risk factor for osteoporosis decades ago. Several studies have shown a direct relationship between cigarette smoking, decreased bone mineral density, and impaired fracture healing. However, the mechanisms behind impaired fracture healing and cigarette smoking are yet to...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2018/3172480

    authors: Aspera-Werz RH,Ehnert S,Heid D,Zhu S,Chen T,Braun B,Sreekumar V,Arnscheidt C,Nussler AK

    更新日期:2018-11-06 00:00:00

  • Radioprotective Effect of Grape Seed Proanthocyanidins In Vitro and In Vivo.

    abstract::We have demonstrated that grape seed proanthocyanidins (GSPs) could effectively scavenge hydroxyl radical (•OH) in a dose-dependent manner. Since most of the ionizing radiation- (IR-) induced injuries were caused by •OH, this study was to investigate whether GSPs would mitigate IR-induced injuries in vitro and in vivo...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2016/5706751

    authors: Huang Y,Zhao H,Cao K,Sun D,Yang Y,Liu C,Cui J,Cheng Y,Li B,Cai J,Gao F

    更新日期:2016-01-01 00:00:00

  • Acute exercise induced mitochondrial H₂O₂ production in mouse skeletal muscle: association with p(66Shc) and FOXO3a signaling and antioxidant enzymes.

    abstract::Exercise induced skeletal muscle phenotype change involves a complex interplay between signaling pathways and downstream regulators. This study aims to investigate the effect of acute exercise on mitochondrial H2O2 production and its association with p(66Shc), FOXO3a, and antioxidant enzymes. Male ICR/CD-1 mice were s...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2015/536456

    authors: Wang P,Li CG,Qi Z,Cui D,Ding S

    更新日期:2015-01-01 00:00:00

  • Metabolomic Analysis Reveals That the Mechanism of Astaxanthin Improves the Osteogenic Differentiation Potential in Bone Marrow Mesenchymal Stem Cells.

    abstract::At present, little research has been done on the metabolic phenotype of the differentiation of mesenchymal stem cells (MSCs) into osteoblasts. In this study, the effect of astaxanthin on improving osteogenic differentiation potential of mesenchymal stem cells was studied by metabolomics. Results showed that L-methioni...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2020/3427430

    authors: Zhao G,Zhong H,Rao T,Pan Z

    更新日期:2020-03-25 00:00:00

  • Iron Redox Chemistry and Implications in the Parkinson's Disease Brain.

    abstract::The etiology of Parkinson's disease (PD) is linked with cellular inclusions in the substantia nigra pars compacta region of the brain that are enriched in the misfolded presynaptic protein α-synuclein (αS) and death of the dopaminergic neurons. Brain iron homeostasis governs both neurotransmission and neurodegeneratio...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章,评审

    doi:10.1155/2019/4609702

    authors: Abeyawardhane DL,Lucas HR

    更新日期:2019-10-09 00:00:00

  • The Antiaggregative and Antiamyloidogenic Properties of Nanoparticles: A Promising Tool for the Treatment and Diagnostics of Neurodegenerative Diseases.

    abstract::Due to the progressive aging of the society, the prevalence and socioeconomic burden of neurodegenerative diseases are predicted to rise. The most common neurodegenerative disorders nowadays, such as Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis, can be classified as proteinopathies. They...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章,评审

    doi:10.1155/2020/3534570

    authors: Pichla M,Bartosz G,Sadowska-Bartosz I

    更新日期:2020-10-13 00:00:00

  • Blueberry Extracts as a Novel Approach to Prevent Ozone-Induced Cutaneous Inflammasome Activation.

    abstract::The World Health Organization estimates that 7 million people die every year due to pollution exposure. Among the different pollutants to which living organism are exposed, ozone (O3) represents one of the most toxic, because its location which is the skin is one of the direct tissues exposed to the outdoor environmen...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2020/9571490

    authors: Pambianchi E,Ferrara F,Pecorelli A,Woodby B,Grace M,Therrien JP,Lila MA,Valacchi G

    更新日期:2020-08-13 00:00:00

  • Corrigendum to "Paricalcitol Pretreatment Attenuates Renal Ischemia-Reperfusion Injury via Prostaglandin E2 Receptor EP4 Pathway".

    abstract::[This corrects the article DOI: 10.1155/2017/5031926.]. ...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 已发布勘误

    doi:10.1155/2017/1913954

    authors: Hong YA,Yang KJ,Jung SY,Park KC,Choi H,Oh JM,Lee SJ,Chang YK,WheePark C,Yang CW,Kim SY,Hwang HS

    更新日期:2017-01-01 00:00:00

  • The Use of Nutraceuticals to Counteract Atherosclerosis: The Role of the Notch Pathway.

    abstract::Despite the currently available pharmacotherapies, today, thirty percent of worldwide deaths are due to cardiovascular diseases (CVDs), whose primary cause is atherosclerosis, an inflammatory disorder characterized by the buildup of lipid deposits on the inside of arteries. Multiple cellular signaling pathways have be...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章,评审

    doi:10.1155/2019/5470470

    authors: Aquila G,Marracino L,Martino V,Calabria D,Campo G,Caliceti C,Rizzo P

    更新日期:2019-05-02 00:00:00

  • Influence of Insulin Resistance and TNF-α on the Inflammatory Process, Oxidative Stress, and Disease Activity in Patients with Rheumatoid Arthritis.

    abstract::The aim of this study was to evaluate the involvement of TNF-α and insulin resistance (IR) in the inflammatory process, oxidative stress, and disease activity in patients with rheumatoid arthritis (RA). This cross-sectional study included 270 subjects (control group, n = 97) and RA patients (n = 173). RA patients were...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2016/8962763

    authors: Costa NT,Veiga Iriyoda TM,Kallaur AP,Delongui F,Alfieri DF,Lozovoy MA,Amin RB,Delfino VD,Dichi I,Simão AN

    更新日期:2016-01-01 00:00:00

  • Mitochondrial DNA instability in cells lacking aconitase correlates with iron citrate toxicity.

    abstract::Aconitase, the second enzyme of the tricarboxylic acid cycle encoded by ACO1 in the budding yeast Saccharomyces cerevisiae, catalyzes the conversion of citrate to isocitrate. aco1Δ results in mitochondrial DNA (mtDNA) instability. It has been proposed that Aco1 binds to mtDNA and mediates its maintenance. Here we prop...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2013/493536

    authors: Farooq MA,Pracheil TM,Dong Z,Xiao F,Liu Z

    更新日期:2013-01-01 00:00:00

  • Increased Oxidative Damage of RNA in Early-Stage Nephropathy in db/db Mice.

    abstract::To evaluate RNA oxidation in the early stage of diabetic nephropathy, we applied an accurate method based on isotope dilution high-performance liquid chromatography-triple quadruple mass spectrometry to analyze the oxidatively generated guanine nucleosides in renal tissue and urine from db/db mice of different ages. W...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2017/2353729

    authors: Wang WX,Luo SB,Jiang P,Xia MM,Hei AL,Mao YH,Li CB,Hu GX,Cai JP

    更新日期:2017-01-01 00:00:00

  • Calenduloside E Ameliorates Myocardial Ischemia-Reperfusion Injury through Regulation of AMPK and Mitochondrial OPA1.

    abstract::Calenduloside E (CE) is a natural triterpenoid saponin isolated from Aralia elata (Miq.) Seem., a well-known traditional Chinese medicine. Our previous studies have shown that CE exerts cardiovascular protective effects both in vivo and in vitro. However, its role in myocardial ischemia/reperfusion injury (MIRI) and t...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2020/2415269

    authors: Wang M,Wang RY,Zhou JH,Xie XH,Sun GB,Sun XB

    更新日期:2020-08-31 00:00:00

  • Xanthine Oxidoreductase-Derived Reactive Species: Physiological and Pathological Effects.

    abstract::Xanthine oxidoreductase (XOR) is the enzyme that catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid and is widely distributed among species. In addition to this housekeeping function, mammalian XOR is a physiological source of superoxide ion, hydrogen peroxide, and nitric oxide, which can fu...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章,评审

    doi:10.1155/2016/3527579

    authors: Battelli MG,Polito L,Bortolotti M,Bolognesi A

    更新日期:2016-01-01 00:00:00

  • Corrigendum to "Protective Effects and Possible Mechanisms of Ergothioneine and Hispidin against Methylglyoxal-Induced Injuries in Rat Pheochromocytoma Cells".

    abstract::[This corrects the article DOI: 10.1155/2017/4824371.]. ...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 已发布勘误

    doi:10.1155/2019/2029096

    authors: Song TY,Yang NC,Chen CL,Thi TLV

    更新日期:2019-08-06 00:00:00

  • Role of peroxiredoxin III in the pathogenesis of pre-eclampsia as evidenced in mice.

    abstract::As a member of peroxiredoxin (Prx) family, PrxIII has been demonstrated to play an important role in scavenging intracellular reactive oxygen species (ROS). Since PrxIII knockout mice exhibited oxidative stress in placentas resembling pathophysiologic changes in placentas of human pre-eclampsia, we measured blood pres...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.4161/oxim.3.1.10094

    authors: Li L,Obinata M,Hori K

    更新日期:2010-01-01 00:00:00

  • Counteraction of Oxidative Stress by Vitamin E Affects Epigenetic Regulation by Increasing Global Methylation and Gene Expression of MLH1 and DNMT1 Dose Dependently in Caco-2 Cells.

    abstract::Obesity- or diabetes-induced oxidative stress is discussed as a major risk factor for DNA damage. Vitamin E and many polyphenols exhibit antioxidative activities with consequences on epigenetic regulation of inflammation and DNA repair. The present study investigated the counteraction of oxidative stress by vitamin E ...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2018/3734250

    authors: Zappe K,Pointner A,Switzeny OJ,Magnet U,Tomeva E,Heller J,Mare G,Wagner KH,Knasmueller S,Haslberger AG

    更新日期:2018-03-22 00:00:00

  • Mito-TEMPO Alleviates Renal Fibrosis by Reducing Inflammation, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress.

    abstract:Background:Renal fibrosis is a common pathological symptom of chronic kidney disease (CKD). Many studies support that mitochondrial dysfunction and endoplasmic reticulum (ER) stress are implicated in the pathogenesis of CKD. In our study, we investigated the benefits and underlying mechanisms of Mito-TEMPO on renal fib...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2018/5828120

    authors: Liu Y,Wang Y,Ding W,Wang Y

    更新日期:2018-03-25 00:00:00

  • Mitochondrial dysfunction: different routes to Alzheimer's disease therapy.

    abstract::Mitochondria are dynamic ATP-generating organelle which contribute to many cellular functions including bioenergetics processes, intracellular calcium regulation, alteration of reduction-oxidation potential of cells, free radical scavenging, and activation of caspase mediated cell death. Mitochondrial functions can be...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章,评审

    doi:10.1155/2014/780179

    authors: Picone P,Nuzzo D,Caruana L,Scafidi V,Di Carlo M

    更新日期:2014-01-01 00:00:00

  • The Degree of Helicobacter pylori Infection Affects the State of Macrophage Polarization through Crosstalk between ROS and HIF-1α.

    abstract:Methods:The expression of CD86, CD206, and HIF-1α in the gastric mucosa was evaluated through immunohistochemistry. RAW 264.7 cells were cocultured with H. pylori at various multiplicities of infection (MOIs), and iNOS, CD86, Arg-1, CD206, and HIF-1α expression was detected by Western blot, PCR, and ELISA analyses. ROS...

    journal_title:Oxidative medicine and cellular longevity

    pub_type: 杂志文章

    doi:10.1155/2020/5281795

    authors: Lu Y,Rong J,Lai Y,Tao L,Yuan X,Shu X

    更新日期:2020-12-08 00:00:00