Abstract:
:We present the spatiotemporal metabolic differentiation of yeast cell subpopulations from upper, lower, and margin regions of colonies of different ages, based on comprehensive transcriptomic analysis. Furthermore, the analysis was extended to include smaller cell subpopulations identified previously by microscopy within fully differentiated U and L cells of aged colonies. New data from RNA-seq provides both spatial and temporal information on cell metabolic reprogramming during colony ageing and shows that cells at marginal positions are similar to upper cells, but both these cell types are metabolically distinct from cells localized to lower colony regions. As colonies age, dramatic metabolic reprogramming occurs in cells of upper regions, while changes in margin and lower cells are less prominent. Interestingly, whereas clear expression differences were identified between two L cell subpopulations, U cells (which adopt metabolic profiles, similar to those of tumor cells) form a more homogeneous cell population. The data identified crucial metabolic reprogramming events that arise de novo during colony ageing and are linked to U and L cell colony differentiation and support a role for mitochondria in this differentiation process.
journal_name
Oxid Med Cell Longevjournal_title
Oxidative medicine and cellular longevityauthors
Wilkinson D,Maršíková J,Hlaváček O,Gilfillan GD,Ježková E,Aaløkken R,Váchová L,Palková Zdoi
10.1155/2018/4932905subject
Has Abstractpub_date
2018-01-11 00:00:00pages
4932905eissn
1942-0900issn
1942-0994journal_volume
2018pub_type
杂志文章abstract:Background:Acute kidney injury (AKI) is one of the common complications of sepsis. Heretofore, there is no effective treatment for septic AKI. Recent studies have revealed that besides treating hematological malignancies, human umbilical cord blood mononuclear cells (hUCBMNCs) show good therapeutic effects on other dis...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/6123459
更新日期:2020-07-28 00:00:00
abstract::Hypoxic-ischemic encephalopathy (HIE) is detrimental to newborns and is associated with high mortality and poor prognosis. Thus, the primary aim of the present study was to determine whether glycine could (1) attenuate HIE injury in rats and hypoxic stress in PC12 cells and (2) downregulate mitochondria-mediated autop...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/4248529
更新日期:2019-02-06 00:00:00
abstract:Purpose:Oxidative stress induced by reduced blood circulation is a critical pathological damage to retinal ganglion cells (RGCs) in glaucoma. We previously showed that green tea extract (GTE) and its catechin constituents alleviate sodium iodate-induced retinal degeneration in rats. Here, we investigated the therapeuti...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/8407206
更新日期:2019-07-09 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease that results from the death of upper and lower motor neurons. Due to a lack of effective treatment, it is imperative to understand the underlying mechanisms and processes involved in disease progression. Regulations in cellular reduction/oxidation (red...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2013/408681
更新日期:2013-01-01 00:00:00
abstract::We have demonstrated that neural progenitor cells (NPCs) protect endothelial cells (ECs) from oxidative stress. Since exosomes (EXs) can convey the benefit of parent cells through their carried microRNAs (miRs) and miR-210 is ubiquitously expressed with versatile functions, we investigated the role of miR-210 in the e...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2017/9397631
更新日期:2017-01-01 00:00:00
abstract::Accumulation of advanced glycation end products (AGEs) contributes to ageing and age-related diseases, especially type 2 diabetes. The NLRP3 inflammasome, as a vital component of the innate immune system, is implicated in the pathogenesis of type 2 diabetes. However, the role of the NLRP3 inflammasome in AGE-induced p...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2017/9692546
更新日期:2017-01-01 00:00:00
abstract:Objective:Glaucoma is a leading cause of severe visual impairment and blindness. Although high intraocular pressure (IOP) is an established risk factor for the disease, the role of abnormal ocular vessel function in the pathophysiology of glaucoma gains more and more attention. We tested the hypothesis that elevated in...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/9736047
更新日期:2019-12-29 00:00:00
abstract::Depression is an inflammatory-related condition, with the progression in neuronal damage resulting in major depression disorder. Ginsenoside-Rg1, a sterol extract from the herb Panax ginseng, has been shown to exert neuroprotective effects upon neurodegeneration disorders. However, whether ginsenoside-Rg1 confers anti...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/2325391
更新日期:2020-03-18 00:00:00
abstract::Stem cells derived from elderly donors or harvested by repeated subculture exhibit a marked decrease in proliferative capacity and multipotency, which not only compromises their therapeutic potential but also raises safety concerns for regenerative medicine. NANOG-a well-known core transcription factor-plays an import...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/4286213
更新日期:2019-12-04 00:00:00
abstract::Uncoupling protein 2 (UCP2) has a cardioprotective role under septic conditions, but the underlying mechanism remains unclear. This study aimed at investigating the effects of UCP2 on the oxidative stress and apoptosis of cardiomyocytes induced by lipopolysaccharide (LPS). First, LPS increased UCP2 expression in cardi...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/2758262
更新日期:2019-04-28 00:00:00
abstract::Mitochondrial DNA (mtDNA) damage is associated with the development of cardiovascular diseases. Cardiac aging plays a central role in cardiovascular diseases. There is accumulating evidence linking cardiac aging to mtDNA damage, including mtDNA mutation and decreased mtDNA copy number. Current wisdom indicates that mt...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2020/9423593
更新日期:2020-03-26 00:00:00
abstract::Essential oils of some aromatic plants provide an effective nonmedicinal option to control liver fibrosis. Mentha piperita L. essential oil (MPEO) have been reported to possess protective effects against hepatotoxicity. However, its effect against liver fibrosis remains unknown. The present study investigated the anti...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/4039753
更新日期:2018-04-19 00:00:00
abstract::We have hypothesized that the adaptive response to low doses of ionizing radiation (IR) is mediated by oxidized cell-free DNA (cfDNA) fragments. Here, we summarize our experimental evidence for this model. Studies involving measurements of ROS, expression of the NOX (superoxide radical production), induction of apopto...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2017/9515809
更新日期:2017-01-01 00:00:00
abstract::Inhibition of either P2Y12 receptor or the nucleotide-binding oligomerization domain- (NOD-) like receptor pyrin domain containing 3 (NLRP3) inflammasome provides cardioprotective effects. Here, we investigate whether direct NLRP3 inflammasome inhibition exerts additive effects on myocardial protection induced by the ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/9219825
更新日期:2020-08-03 00:00:00
abstract:Background:Amino acids (AAs) in cerebrospinal fluid (CSF) play a pivotal role in cerebral ischemia (CI). BuChang NaoXinTong Capsules (BNC) are widely prescribed in Chinese medicine for the treatment of cerebrovascular and cardiovascular diseases. Methods:In order to investigate the therapeutic effects and pharmacologi...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/7258624
更新日期:2019-03-20 00:00:00
abstract:Background:Mitochondrial dynamics (mtDYN) has been proposed as a bridge between mitochondrial dysfunction and insulin resistance (IR), which is involved in the pathogenesis of type 2 diabetes (T2D). Our previous study has identified that mitochondrial DNA (mtDNA) haplogroup B4 is a T2D-susceptible genotype. Using trans...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/7514383
更新日期:2018-09-30 00:00:00
abstract::Nitro-oxidative stress (NOS) plays a fundamental role in aging, as well as in the pathogenesis of neurodegenerative disorders, and major depression (MD). The latter is a very frequent psychiatric illness characterized by accelerated aging, neurodegeneration, high comorbidity with age-related disorders, and premature m...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2013/230797
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To explore the protective effects of hydrogen-rich saline on rats with smoke inhalation injury. METHODS:36 healthy male Sprague-Dawley rats were randomly divided into 3 groups (n = 12 per group): sham group (S), inhalation injury plus normal saline treatment group (I+NS), and inhalation injury plus hydrogen-...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2015/106836
更新日期:2015-01-01 00:00:00
abstract::We investigated the effect of high-frequency electromagnetic fields (HF-EMFs) and 17-β-estradiol on connexins (Cxs), integrins (Ints), and estrogen receptor (ER) expression, as well as on ultrastructure of trophoblast-derived HTR-8/SVneo cells. HF-EMF, 17-β-estradiol, and their combination induced an increase of Cx40 ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2013/280850
更新日期:2013-01-01 00:00:00
abstract::Reactive oxygen species (ROS) production in hepatic ischemia-reperfusion injury (IRI) is a complex process where multiple cellular and molecular pathways are involved. Few of those molecular pathways are under the direct influence of SIRT3 and its downstream mediators. SIRT3 plays a major role in the mechanism of IRI,...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2018/2976957
更新日期:2018-11-13 00:00:00
abstract::Detection of free radicals in tissues is challenging. Most approaches rely on incubating excised sections or homogenates with reagents, typically at supraphysiologic oxygen tensions, to finally detect surrogate, nonspecific end products. In the present work, we explored the potential of using intravenously (i.v.) inje...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/7838406
更新日期:2019-05-22 00:00:00
abstract::Plants of the Euphorbia genus present a wide range of therapeutic applications. This study is aimed at investigating new antidigestive enzyme agents from Euphorbia cyparissias through inhibition of lipid and carbohydrate absorption, to evaluate their potential applications for the treatment of metabolic syndrome. Lipa...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/9835167
更新日期:2020-10-29 00:00:00
abstract:Objective:Oxidized cholesterol derivatives are compounds with proven atherogenic and mutagenic effects. However, little is known about the effect of oxidized plant sterol derivatives (oxyphytosterols), whose structure is similar to the one of oxycholesterols. Our previous studies indicate that they have a similar profi...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/4751803
更新日期:2020-06-08 00:00:00
abstract::2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG) is active component of the Chinese medicinal plant Polygonum multiflorum Thunb. (THSG). Pharmacological studies have demonstrated that THSG exhibits numerous biological functions in treating atherosclerosis, lipid metabolism, vascular and cardiac remodeling, vascu...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2016/4973239
更新日期:2016-01-01 00:00:00
abstract::Ischemic stroke remains the fifth cause of death, as reported worldwide annually. Endothelial dysfunction (ED) manifesting with lower nitric oxide (NO) bioavailability leads to increased vascular tone, inflammation, and platelet activation and remains among the major contributors to cardiovascular diseases (CVD). More...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2020/2979260
更新日期:2020-08-28 00:00:00
abstract::Hormone replacement therapy (HRT) can alleviate estrogen deficiency symptoms especially during menopause. The present study aimed at investigating the effect of soy isoflavones as HRT on immunological and bone health-related parameters with a special focus on the interactions between immunological status and metabolis...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/5713606
更新日期:2019-01-10 00:00:00
abstract::The total lifespan of the yeast Saccharomyces cerevisiae may be divided into two phases: the reproductive phase, during which the cell undergoes mitosis cycles to produce successive buds, and the postreproductive phase, which extends from the last division to cell death. These phases may be regulated by a common mecha...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/1898421
更新日期:2018-03-20 00:00:00
abstract::As a core member of p38 MAPK signal transduction pathway, p38 regulated/activated kinase (PRAK) is activated by cellular stresses. However, the function of PRAK and its downstream interacting partner remain undefined. Using a yeast two-hybrid system, we identified DJ-1 as a potential PRAK interacting protein. We furth...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2014/735618
更新日期:2014-01-01 00:00:00
abstract::Benzene is a chemical contaminant widespread in industrial and living environments. The oxidative metabolites of benzene induce toxicity involving oxidative damage. Protecting cells and cell membranes from oxidative damage, glucose-6-phosphate dehydrogenase (G6PD) maintains the reduced state of glutathione (GSH). This...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/3912515
更新日期:2016-01-01 00:00:00
abstract::The generation of reactive oxygen and nitrogen species (RONS) has been found to occur during inflammatory procedures, during cell ischemia, and in various crucial developmental processes such as cell differentiation and along cell signaling pathways. The most common sources of intracellular RONS are the mitochondrial ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2017/7542540
更新日期:2017-01-01 00:00:00