Abstract:
:For mammals, oxalate enters the body through the diet or is endogenously produced by the liver; it is removed by microbial oxalate metabolism in the gut and/or excretion in feces or urine. Deficiencies in any one of the these pathways can lead to complications, such as calcium oxalate urinary stones. While considerable research has been conducted on individual oxalate-degrading bacterial isolates, interactions between oxalate and the gut microbiota as a whole are unknown. We examined the reduction in oxalate excretion in a rat model following oral administration of fecal microbes from a mammalian herbivore adapted to a high oxalate diet or to fecal transplants consisting of two different formulations of mixed oxalate-degrading isolates. While all transplants elicited a significant reduction in oxalate excretion initially, the greatest effect was seen with fecal microbial transplants, which persisted even in the absence of dietary oxalate. The reduction in oxalate excretion in animals given fecal transplants corresponded with the establishment of diverse bacteria, including known oxalate-degrading bacteria and a cohesive network of bacteria centered on oxalate-degrading specialists from the Oxalobacteraceae family. Results suggested that the administration of a complete community of bacteria facilitates a cohesive balance in terms of microbial interactions. Our work offers important insights into the development of targeted bacteriotherapies intended to reduce urinary oxalate excretion in patients at risk for recurrent calcium oxalate stones as well as bacteriotherapies targeting other toxins for elimination. IMPORTANCE Oxalate is a central component in 80% of kidney stones. While mammals do not possess the enzymes to degrade oxalate, many gastrointestinal bacteria are efficient oxalate degraders. We examined the role of cohesive microbial networks for oxalate metabolism, using Sprague-Dawley rats as a model host. While the transplantation of oxalate-degrading bacteria alone to the Sprague-Dawley hosts did increase oxalate metabolism, fecal transplants from a wild mammalian herbivore, Neotoma albigula, had a significantly greater effect. Furthermore, the boost for oxalate metabolism persisted only in animals that received fecal transplants. Animals receiving fecal transplants had a more diverse and cohesive network of bacteria associated with the Oxalobacteraceae, a family known to consist of specialist oxalate-degrading bacteria, than did animals that received oxalate-degrading bacteria alone. Our results indicate that fecal transplants are more effective at transferring specific functions than are microbial specialists alone, which has broad implications for the development of bacteriotherapies.
journal_name
mSystemsjournal_title
mSystemsauthors
Miller AW,Dale C,Dearing MDdoi
10.1128/mSystems.00088-17subject
Has Abstractpub_date
2017-09-26 00:00:00issue
5issn
2379-5077pii
mSystems00088-17journal_volume
2pub_type
杂志文章相关文献
mSystems文献大全abstract::Globally, dental caries is the most prevalent chronic oral disease and affects roughly half of all children. The aim of this report was to use metagenomic analyses to investigate the relationship between the oral microbiome and caries in preschool children. A total of 25 preschoolers, aged 3 to 5 years old with severe...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00450-19
更新日期:2019-11-05 00:00:00
abstract::Classified as a biosafety level 4 (BSL4) select agent, Nipah virus (NiV) is a deadly henipavirus in the Paramyxoviridae family, with a nearly 75% mortality rate in humans, underscoring its global and animal health importance. Elucidating the process of viral particle production in host cells is imperative both for tar...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00194-19
更新日期:2019-09-24 00:00:00
abstract::Trauma patients (TPs) are highly susceptible to infections, which often lead to sepsis. Among the numerous causative agents, Pseudomonas aeruginosa is especially important, as P. aeruginosa sepsis is often fatal. Understanding the mechanism of its pathogenesis in bloodstream infections is imperative; however, this mec...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00312-18
更新日期:2019-05-07 00:00:00
abstract::The intestinal microbiota and the expanded endocannabinoid (eCB) system, or endocannabinoidome (eCBome), have both been implicated in diet-induced obesity and dysmetabolism. These systems were recently suggested to interact during the development of obesity. We aimed at identifying the potential interactions between g...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00407-19
更新日期:2019-12-17 00:00:00
abstract::Arctic regions, which are changing rapidly as they warm 2 to 3 times faster than the global average, still retain microbial habitats that serve as natural laboratories for understanding mechanisms of microbial adaptation to extreme conditions. Seawater-derived brines within both sea ice (sea-ice brine) and ancient lay...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00246-20
更新日期:2020-06-16 00:00:00
abstract::Polymyxins are a last-line therapy against multidrug-resistant Pseudomonas aeruginosa; however, resistance to polymyxins has been increasingly reported. Therefore, understanding the mechanisms of polymyxin activity and resistance is crucial for preserving their clinical usefulness. This study employed comparative meta...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00149-18
更新日期:2019-01-08 00:00:00
abstract::Interactions between plant pathogens and root-associated microbes play an important role in determining disease outcomes. While several studies have suggested that steering these interactions may improve plant health, such approaches have remained challenging in practice. Because of low iron availability in most soils...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00811-19
更新日期:2020-06-30 00:00:00
abstract::The reactive intermediate deaminase RidA (EC 3.5.99.10) is conserved across all domains of life and deaminates reactive enamine species. When Salmonella entericaridA mutants are grown in minimal medium, 2-aminoacrylate (2AA) accumulates, damages several pyridoxal 5'-phosphate (PLP)-dependent enzymes, and elicits an ob...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00843-19
更新日期:2020-03-10 00:00:00
abstract::Simplified microbial communities, or "benchtop microbiomes," enable us to manage the profound complexity of microbial ecosystems. Widespread activities aiming to design and control communities result in novel resources for testing ecological theories and also for realizing new biotechnologies. There is much to be gain...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00106-19
更新日期:2019-05-28 00:00:00
abstract::Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients, but the factors that de...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00015-18
更新日期:2018-06-26 00:00:00
abstract::Microbial heterotopic metabolism in the ocean is fueled by a supply of essential nutrients acquired via exoenzymes catalyzing depolymerization of high-molecular-weight compounds. Although the rates of activity for a variety of exoenzymes across various marine environments are well established, the factors regulating t...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00290-20
更新日期:2020-04-14 00:00:00
abstract::Many pathogenic bacteria of the family Enterobacteriaceae use type III secretion systems to inject virulence proteins, termed "effectors," into the host cell cytosol. Although host-cellular activities of several effectors have been demonstrated, the function and host-targeted pathways of most of the effectors identifi...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00032-15
更新日期:2016-07-12 00:00:00
abstract::The intestinal microbiota contains trillions of commensal microorganisms that shape multiple aspects of host physiology and disease. In contrast to the host's genome, the microbiome is amenable to change over the course of an organism's lifetime, providing an opportunity to therapeutically modulate the microbiome's im...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00216-18
更新日期:2019-02-19 00:00:00
abstract::In pregnant and lactating sows, metabolism and immunity undergo drastic changes, which can lead to constipation, abortion, and intrauterine growth restriction (IUGR) and reduce production performance. Dietary fiber can regulate animal gut microbiota, alleviate inflammatory responses, and improve performance. Here, 48 ...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00591-20
更新日期:2021-01-26 00:00:00
abstract::Fecal microbiota transplantation (FMT) is a proposedly useful strategy for the treatment of gastrointestinal (GI) disorders through remediation of the patient gut microbiota. However, its therapeutic success has been variable, necessitating research to uncover mechanisms that improve patient response. Antibiotic pretr...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00404-19
更新日期:2020-01-28 00:00:00
abstract::Ambient temperature (Ta ) is an important factor in shaping phenotypic plasticity. Plasticity is generally beneficial for animals in adapting to their environments. Gut microbiota are crucial in regulating host physiological and behavioral processes. However, whether the gut microbiota play a role in regulating host p...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00514-20
更新日期:2020-10-20 00:00:00
abstract::The identification of ancestral traits is essential to understanding the evolution of any group. In the case of parasitic groups, this helps us understand the adaptation to this lifestyle and a particular host. Most diplomonads are parasites, but there are free-living members of the group nested among the host-associa...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00774-20
更新日期:2020-12-22 00:00:00
abstract::Improvements in growth performance and health are key goals in broiler chicken production. Inclusion of prebiotic galacto-oligosaccharides (GOS) in broiler feed enhanced the growth rate and feed conversion of chickens relative to those obtained with a calorie-matched control diet. Comparison of the cecal microbiota id...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00827-19
更新日期:2020-01-14 00:00:00
abstract::The mechanisms by which microbes interact in communities remain poorly understood. Here, we interrogated specific interactions between photoautotrophic and heterotrophic members of a model consortium to infer mechanisms that mediate metabolic coupling and acclimation to partnership. This binary consortium was composed...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00181-16
更新日期:2017-03-07 00:00:00
abstract::Thaumarchaeota are frequently reported to associate with marine sponges (phylum Porifera); however, little is known about the features that distinguish them from their free-living thaumarchaeal counterparts. In this study, thaumarchaeal metagenome-assembled genomes (MAGs) were reconstructed from metagenomic data sets ...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00288-19
更新日期:2019-08-13 00:00:00
abstract::ABSRTACTKlebsiella pneumoniae is a common cause of human-pneumonia-derived sepsis with high morbidity and mortality. The microbiota promotes and maintains host immune homeostasis. The mechanisms by which the gut microbiota affects the host defenses in the respiratory system systematically, however, remain poorly under...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00587-20
更新日期:2020-11-03 00:00:00
abstract::GapMind is a Web-based tool for annotating amino acid biosynthesis in bacteria and archaea (http://papers.genomics.lbl.gov/gaps). GapMind incorporates many variant pathways and 130 different reactions, and it analyzes a genome in just 15 s. To avoid error-prone transitive annotations, GapMind relies primarily on a dat...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00291-20
更新日期:2020-06-23 00:00:00
abstract::Insights into the interaction between phages and their bacterial hosts are crucial for the development of phage therapy. However, only one study has investigated global gene expression of Clostridioides (formerly Clostridium) difficile carrying prophage, and transcriptional reprogramming during lytic infection has not...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00017-20
更新日期:2020-05-05 00:00:00
abstract::Pullorum disease is one of the most common diarrhea-related diseases caused by Salmonella enterica subspecies enterica serovar Gallinarum biovar Pullorum (S Pullorum); it negatively affects the poultry industry. However, limited studies have explored the association between the gut microbiota and S Pullorum infection ...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.01192-20
更新日期:2021-01-05 00:00:00
abstract::Antimicrobial therapies against cystic fibrosis (CF) lung infections are largely aimed at the traditional, well-studied CF pathogens such as Pseudomonas aeruginosa and Burkholderia cepacia complex, despite the fact that the CF lung harbors a complex and dynamic polymicrobial community. A clinical focus on the dominant...
journal_title:mSystems
pub_type: 杂志文章,评审
doi:10.1128/mSystems.00809-20
更新日期:2020-12-01 00:00:00
abstract::A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host, Mycobacterium smegmatis mc2155. In that context, we unveil a novel defense mechanism in cluster N prophage Butters. By using bioinformatics analy...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00534-20
更新日期:2020-10-06 00:00:00
abstract::Networks encode the interactions between the components in complex systems and play an essential role in understanding complex systems. Microbial ecological networks provide a system-level insight for comprehensively understanding complex microbial interactions, which play important roles in microbial community assemb...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00124-19
更新日期:2019-05-21 00:00:00
abstract::Microbiome studies typically focus on characterizing the taxonomic and functional profiles of the microbes within a community. Functional profiling is generally thought to be superior to taxonomic profiling for investigating human-microbe interactions, but there are several limitations and challenges to existing appro...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00163-17
更新日期:2018-03-27 00:00:00
abstract::Advances in sequencing technologies have enabled novel insights into microbial niche differentiation, from analyzing environmental samples to understanding human diseases and informing dietary studies. However, identifying the microbial taxa that differentiate these samples can be challenging. These issues stem from t...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00162-16
更新日期:2017-01-17 00:00:00
abstract::Nutrient acquisition from the host environment is crucial for the survival of intracellular pathogens, but conceptual and technical challenges limit our knowledge of pathogen diets. To overcome some of these technical roadblocks, we exploited an experimentally accessible model for early infection of human macrophages ...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00057-17
更新日期:2017-08-22 00:00:00