Glycerol Dehydratases: Biochemical Structures, Catalytic Mechanisms, and Industrial Applications in 1,3-Propanediol Production by Naturally Occurring and Genetically Engineered Bacterial Strains.

abstract：:An efficient regeneration system was established for an ethnomedicinal shrub Rhinacanthus nasutus from root-derived callus organogenesis. The root segments were cultured on MS medium supplemented with various concentrations of Kn (1.0-4.0 μM) alone or in combination with IBA (0.2-0.6 μM) or 2, 4-D (0.5-1.5 μM). The op...

journal_title：Applied biochemistry and biotechnology

authors： Cheruvathur MK,Thomas TD

更新日期：2014-02-01 00:00:00

abstract：:Different initial cell concentrations of a recombinant flocculent Saccharomyces cerevisiae MA-R4 were evaluated for their effects on xylose fermentation and glucose-xylose cofermentation. A high initial cell concentration greatly increased both the substrate utilization and ethanol production rates. During xylose ferm...

journal_title：Applied biochemistry and biotechnology

authors： Matsushika A,Sawayama S

更新日期：2010-11-01 00:00:00

abstract：:A Bacillus subtilis strain isolated from contaminated soil from a refinery has been screened for biosurfactant production in crystal sugar (sucrose) with different nitrogen sources (NaNO(3), (NH(4))(2)SO(4), urea, and residual brewery yeast). The highest reduction in surface tension was achieved with a 48-h fermentati...

journal_title：Applied biochemistry and biotechnology

authors： Fonseca RR,Silva AJ,De França FP,Cardoso VL,Sérvulo EF

更新日期：2007-04-01 00:00:00

abstract：:Fluorescent proteins from marine organisms represent potential candidates for biosensor development. In this paper, we described the isolation of a native green fluorescent protein from Anemonia sulcata and the cloning and purification of its equivalent as a recombinant protein in Escherichia coli. Furthermore, the sp...

journal_title：Applied biochemistry and biotechnology

authors： Masullo T,Puccio R,Di Pierro M,Tagliavia M,Censi P,Vetri V,Militello V,Cuttitta A,Colombo P

更新日期：2014-02-01 00:00:00

abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...

journal_title：Applied biochemistry and biotechnology

authors： Filiz E,Koc I,Ozyigit II

更新日期：2014-07-01 00:00:00

abstract：:Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

journal_title：Applied biochemistry and biotechnology

authors： Liu K,Chua H,Lo WH,Lawford H,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:The increase of multidrug-resistant pathogens and the restriction on the use antibiotics due to its side effects have drawn attention to the search for possible alternatives. Bacteriocins are small antimicrobial peptides produced by numerous bacteria. Much interest has been focused on bacteriocins because they exhibit...

journal_title：Applied biochemistry and biotechnology

authors： Jena PK,Trivedi D,Chaudhary H,Sahoo TK,Seshadri S

更新日期：2013-04-01 00:00:00

abstract：:Sweet sorghum (Sorghum sp.) has high biomass yield. Hydrolysis of lignocellulosic sweet sorghum bagasse (SSB) to fermentable sugar could be useful for manufacture of biofuel or other fermentation products. Pretreatment of lignocellulosic biomass to degrade lignin before enzymatic hydrolysis is a key step. Fungal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Mishra V,Jana AK

更新日期：2017-09-01 00:00:00

abstract：:Streptomyces coelicolor A3(2) sco5931 gene was predicted to encode a putative xylanase A, a 477 amino acid protein belonging to glycoside hydrolase family 10. The entire sco5931 coding region was cloned and overexpressed in Streptomyces lividans TK24. Mature SCO5931 protein comprising 436 amino acids (47 kDa) was puri...

journal_title：Applied biochemistry and biotechnology

authors： Enkhbaatar B,Lee CR,Hong YS,Hong SK

更新日期：2016-09-01 00:00:00

abstract：:A newly isolated bacterial strain, Bacillus sp. MX47, was actively producing extracellular xylanase only in xylan-containing medium. The xylanase was purified from the culture broth by two chromatographic steps. The xylanase had an apparent molecular weight of 26.4 kDa with an NH(2)-terminal sequence (Gln-Gly-Gly-Asn-...

journal_title：Applied biochemistry and biotechnology

authors： Chi WJ,Park DY,Chang YK,Hong SK

更新日期：2012-10-01 00:00:00

abstract：:Colour and COD removals of the azo dyes Congo Red (CR) and Reactive Black 5 (RB5) were individually evaluated in a sequential anaerobic/aerobic treatment system. Additionally, dye toxicity was assessed by using acute ecotoxicity tests with Daphnia magna as the indicator-organism. The anaerobic reactor was operated at ...

journal_title：Applied biochemistry and biotechnology

authors： Silva ME,Firmino PI,Sousa MR,Santos AB

更新日期：2012-02-01 00:00:00

abstract：:Sugarcane bagasse is the major by-product of the sugar industry. It has a great potential for the production of biofuels and chemicals due to its considerable amount of cellulose and hemicellulose. In this study, we investigated a simple and economic pretreatment process using dilute ammonia for the storage of sugarca...

journal_title：Applied biochemistry and biotechnology

authors： Kim M,Aita G,Day DF

更新日期：2010-05-01 00:00:00

abstract：:Bacillus amyloliquefaciens LL3 is a glutamate-independent poly-γ-glutamic acid (γ-PGA) producing strain which consists of a circular chromosome (3,995,227 bp) and an endogenous plasmid pMC1 (6,758 bp). The study of the function of native plasmid and the genome-size reduction of the B. amyloliquefaciens LL3 strain requ...

journal_title：Applied biochemistry and biotechnology

authors： Feng J,Gu Y,Wang J,Song C,Yang C,Xie H,Zhang W,Wang S

更新日期：2013-09-01 00:00:00

abstract：:The microheterogeneous native amylolytic complex secreted by the isolate A6 of Lactobacillus plantarum revealed a selective enzyme specificity loss when submitted to a limited proteolysis under a suboptimum pH condition. A clear electrophoretic profile change toward just one shorter, more acidic, and equally active po...

journal_title：Applied biochemistry and biotechnology

authors： Florêncio JA,Eiras-Stofella DR,Soccol CR,Raimbault M,Guyot JP,Fontana JD

更新日期：2000-04-01 00:00:00

abstract：:Batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. The best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g...

journal_title：Applied biochemistry and biotechnology

authors： Rodrigues DC,Silva SS,Prata AM,Felipe MG

更新日期：1998-04-01 00:00:00

abstract：:Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented fo...

journal_title：Applied biochemistry and biotechnology

authors： Rudolf A,Galbe M,Lidén G

更新日期：2004-04-01 00:00:00

abstract：:Fatty acid methyl esters (FAMEs) show large potential applications as diesel substitutes, also known as biodiesel fuel. Biodiesel fuel as renewable energy is an alternative that can reduce energy dependence on petroleum as well as air pollution. Several processes for the production of biodiesel fuel have been develope...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH,Kang CH,Lee WT,Sunwoo CS,Yoon CH,Choi BC,Kim HS,Kim SW,Lee UT

更新日期：2004-04-01 00:00:00

abstract：:The polysaccharides extracted from the achenes of jelly fig, Ficus awkeotsang Makino, were mainly composed of low methyl pectin and used as a novel shell material for encapsulating lipophilic bioactives in the core of microcapsule. The polysaccharide microcapsules with oil core were prepared using a novel acrylic-base...

journal_title：Applied biochemistry and biotechnology

authors： Ponrasu T,Yang RF,Chou TH,Wu JJ,Cheng YS

更新日期：2020-05-01 00:00:00

abstract：:This study investigated methane production in an anaerobic sequencing batch biofilm reactor (AnSBBR) by co-digesting sugarcane vinasse and cheese whey. The assessment was based on the influence of feed strategy, interaction between cycle time and influent concentration, applied volumetric organic load (OLRA), and temp...

journal_title：Applied biochemistry and biotechnology

authors： Sousa SP,Lovato G,Albanez R,Ratusznei SM,Rodrigues JAD

更新日期：2019-11-01 00:00:00

abstract：:Empty palm fruit bunch fiber (EPFBF), a readily available cellulosic biomass from palm processing facilities, is investigated as a potential carbohydrate source for cellulosic ethanol production. This feedstock was pretreated using ammonia fiber expansion (AFEX) and enzymatically hydrolyzed. The best tested AFEX condi...

journal_title：Applied biochemistry and biotechnology

authors： Lau MJ,Lau MW,Gunawan C,Dale BE

更新日期：2010-11-01 00:00:00

abstract：:The semi-continuous anaerobic digestion of food waste was investigated in 1-L and 20-L continuously stirred tank reactors (CSTRs), to identify the optimum operation condition and the methane production of the semi-continuous anaerobic process. Results from a 1-L digester indicated that the optimum organic loading rate...

journal_title：Applied biochemistry and biotechnology

authors： Zhang C,Su H,Wang Z,Tan T,Qin P

更新日期：2015-04-01 00:00:00

abstract：:A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively...

journal_title：Applied biochemistry and biotechnology

authors： Takuma S,Nakashima N,Tantirungkij M,Kinoshita S,Okada H,Seki T,Yoshida T

更新日期：1991-04-01 00:00:00

abstract：:To determine the influence of various culture conditions, transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were grown in nine cultures with four variable conditions (storage of inoculated broth at 4 degrees C prior to incubation, agitation speed, isopropyl-beta-D-thiogalac...

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Ishii M,Junior AP,de Oliveira Nascimento L,de Souza LC,Cholewa O

更新日期：2004-04-01 00:00:00

abstract：:Fructose-1,6-bisphosphate (F-1,6-P2) is an allosteric activator of two key enzymes of glycolysis: phosphofructokinase and pyruvate kinase. Regulation of glycolysis in a wild-type Saccharomyces cerevisiae and a recombinant Escherichia coli by a dead-end structural analog of F-1,6-P2 was studied. 2,5-Anhydromannitol (2,...

journal_title：Applied biochemistry and biotechnology

authors： Nghiem NP,Cofer TM

更新日期：2007-05-01 00:00:00

abstract：:Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Marques M,Machoshvili IA,Ishii M

更新日期：2002-04-01 00:00:00

abstract：:A strain of Clostridium thermoaceticum (ATCC 49707) was evaluated for its homoacetate potential. This thermophilic anaerobe best produces acetate from glucose at pH 6.0 and 59 degrees C with a yield of 83% of theoretical. Enzyme hydrolysis of two substrates, a-cellulose and a pulp mill sludge, yielded 68% and 70% dige...

journal_title：Applied biochemistry and biotechnology

authors： Borden JR,Lee YY,Yoon HH

更新日期：2000-04-01 00:00:00

abstract：:Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...

journal_title：Applied biochemistry and biotechnology

authors： Bollók M,Réczey K,Zacchi G

更新日期：2000-04-01 00:00:00

abstract：:Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enzyme properties by i...

journal_title：Applied biochemistry and biotechnology

authors： Wei J,Zhou Y,Xu T,Lu B

更新日期：2010-09-01 00:00:00

abstract：:Using microalgae to capture CO2 from flue gas is an ideal way to reduce CO2 emission, but this is challenged by the high cost of carbon capture and transportation. To address this problem, a bicarbonate-based integrated carbon capture and algae production system (BICCAPS) has been proposed, in which bicarbonate is use...

journal_title：Applied biochemistry and biotechnology

authors： Chi Z,Elloy F,Xie Y,Hu Y,Chen S

更新日期：2014-01-01 00:00:00

abstract：:Bioethanol is considered a potential liquid fuel that can be produced from biomass by fermentation and distillation. Although most of the water is removed by distillation, the purity of ethanol is limited to 95-96 % due to the formation of a low-boiling point, water-ethanol azeotrope. To improve the use of ethanol as ...

journal_title：Applied biochemistry and biotechnology

authors： Tang B,Bi W,Row KH

更新日期：2013-02-01 00:00:00