Abstract：
OBJECTIVE:To clarify the clinical utility of urinary DNA methylation for detection of intravesical recurrence of non-muscle invasive BCa (NMIBC), we performed a 2-center prospective study. PATIENTS AND METHODS:A series of 207 self-voided urine samples were prospectively collected from 132 patients with NMIBC who had undergone transurethral resection of BCa. Methylation of miRNA genes (miR-9-3, miR-124-2, miR-124-3, and miR-137) was analyzed using bisulfite pyrosequencing. The primary end point was detection of intravesical recurrence; the secondary end point was prediction of late recurrence. The number of methylated genes (M-score) or quantitative level of methylation were compared with outcomes. RESULTS:Twenty-six urine specimens were collected on the same day intravesical recurrence was detected, and 14 were collected from patients whose recurrences were found during the subsequent follow-up period (0-632 days, mean, 342.2 days). For detection of current recurrence, M-scores achieved 61.5% sensitivity and 74.0% specificity, and the area under the ROC curve was 0.71. Regarding prediction of late recurrence, patients with a high M-score (≥3) showed worse recurrence-free survival (P <.01). Multivariate analysis revealed that high M-scores were independently associated with current (P = .028) and late recurrence (P = .026). Elevated levels of urinary DNA methylation were also strongly associated with recurrence and radical cystectomy. CONCLUSION:Our data suggest that urinary methylation of miRNA genes may be a useful marker for detecting and predicting BCa recurrence.
journal_name
Urologyjournal_title
Urologyauthors
Shindo T,Shimizu T,Nojima M,Niinuma T,Maruyama R,Kitajima H,Kai M,Itoh N,Suzuki H,Masumori Ndoi
10.1016/j.urology.2017.11.025subject
Has Abstractpub_date
2018-03-01 00:00:00pages
71-78eissn
0090-4295issn
1527-9995pii
S0090-4295(17)31223-2journal_volume
113pub_type
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