Multiplexed color-coded probe-based gene expression assessment for clinical molecular diagnostics in formalin-fixed paraffin-embedded human renal allograft tissue.

Abstract:

:Histopathologic diagnoses in transplantation can be improved with molecular testing. Preferably, molecular diagnostics should fit into standard-of-care workflows for transplant biopsies, that is, formalin-fixed paraffin-embedded (FFPE) processing. The NanoString(®) gene expression platform has recently been shown to work with FFPE samples. We aimed to evaluate its methodological robustness and feasibility for gene expression studies in human FFPE renal allograft samples. A literature-derived antibody-mediated rejection (ABMR) 34-gene set, comprised of endothelial, NK cell, and inflammation transcripts, was analyzed in different retrospective biopsy cohorts and showed potential to molecularly discriminate ABMR cases, including FFPE samples. NanoString(®) results were reproducible across a range of RNA input quantities (r = 0.998), with different operators (r = 0.998), and between different reagent lots (r = 0.983). There was moderate correlation between NanoString(®) with FFPE tissue and quantitative reverse transcription polymerase chain reaction (qRT-PCR) with corresponding dedicated fresh-stabilized tissue (r = 0.487). Better overall correlation with histology was observed with NanoString(®) (r = 0.354) than with qRT-PCR (r = 0.146). Our results demonstrate the feasibility of multiplexed gene expression quantification from FFPE renal allograft tissue. This represents a method for prospective and retrospective validation of molecular diagnostics and its adoption in clinical transplantation pathology.

journal_name

Clin Transplant

journal_title

Clinical transplantation

authors

Adam B,Afzali B,Dominy KM,Chapman E,Gill R,Hidalgo LG,Roufosse C,Sis B,Mengel M

doi

10.1111/ctr.12689

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

295-305

issue

3

eissn

0902-0063

issn

1399-0012

journal_volume

30

pub_type

杂志文章
  • Non-invasive ventilation in prone position for refractory hypoxemia after bilateral lung transplantation.

    abstract::Temporary graft dysfunction with gas exchange abnormalities is a common finding during the postoperative course of a lung transplant and is often determined by the post-reimplantation syndrome. Supportive measures including oxygen by mask, inotropes, diuretics, and pulmonary vasodilators are usually effective in non-s...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2009.01050.x

    authors: Feltracco P,Serra E,Barbieri S,Persona P,Rea F,Loy M,Ori C

    更新日期:2009-09-01 00:00:00

  • Intra-operative cardiac arrests during liver transplantation - a retrospective review of the first 15 yr in Asian population.

    abstract::Although many report intra-operative cardiac arrests (ICAs) in liver transplantation (LT), the incidence, major causes, and outcome remain unclear. We aimed to investigate retrospectively, the incidence, nature, and outcome of ICA in Asian population and to identify risk factors for ICA. Consecutive 1071 LTs in an ins...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/ctr.12085

    authors: Lee SH,Gwak MS,Choi SJ,Shin YH,Ko JS,Kim GS,Lee SY,Kim MH,Park HG,Lee SK,Jeon HJ

    更新日期:2013-03-01 00:00:00

  • Heparin-induced thrombocytopenia following pancreatectomy and islet auto-transplantation.

    abstract::We report the case of a female with chronic pancreatitis who underwent multiple surgical procedures leading to total resection of the pancreas with successful islet auto-transplantation. Postoperatively, she developed heparin-induced thrombocytopenia. Heparinization prior to islet infusion is currently recommended to ...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2005.00457.x

    authors: Rastellini C,Brown ML,Cicalese L

    更新日期:2006-03-01 00:00:00

  • Methamphetamine use in deceased kidney donors impairs one-yr graft function.

    abstract:BACKGROUND:Methamphetamine (MA) has cumulative deleterious effects on multiple organ systems. A history of MA exposure in kidney donors may affect adversely graft function in recipients. METHODS:Between September 2000 and March 2004, all deceased kidney donors were identified from the local registry (97 donors). Twent...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2007.00703.x

    authors: Inouye DS,Kickertz K,Wong LL

    更新日期:2007-09-01 00:00:00

  • HMGCoA reductase inhibitors and omega-3 fatty acids as adjunctive agents in maintenance immunosuppression after solid organ transplantation.

    abstract::Omega-3 fatty acids have been shown to play a role in preventing development of both acute and chronic rejection. New evidence also suggests that HMGCoA reductase inhibitors may have an immunomodulatory effect and may decrease the incidence of rejection in both animal and human transplant models. Omega-3 fatty acids a...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:

    authors: Katznelson S,Wilkinson AH

    更新日期:1995-06-01 00:00:00

  • Analysis of local versus imported expanded criteria donor kidneys: A single-center experience with 497 ECD kidney transplants.

    abstract:BACKGROUND:The value of importing expanded criteria donor (ECD) kidneys is uncertain. METHODS:We retrospectively reviewed our single-center experience with ECD kidney transplants (KT). RESULTS:Over 12.8 years, we performed 497 ECD KTs including 247 local and 250 imported from other donor service areas. The import ECD...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/ctr.13029

    authors: Khan MA,El-Hennawy H,Farney AC,Rogers J,Orlando G,Reeves-Daniel A,Palanisamy A,Gautreaux M,Iskandar S,Doares W,Kaczmorski S,Stratta RJ

    更新日期:2017-08-01 00:00:00

  • Interstitial alterations in renal cortex in acute tubular necrosis (ATN) post-renal transplantation and in patients with ATN not related to renal transplant.

    abstract:BACKGROUND:Abnormalities of renal function with long-term implications can persist after acute tubular necrosis (ATN), probably because of permanent loss of nephrons. Residual areas of fibrosis are also observed in the renal cortex post-ATN. In this study, we investigate the interstitial alterations post-ATN using hist...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1046/j.1399-0012.2003.00140.x

    authors: Moyses Neto M,Costa RS,Volpini RA,Garcia TM,Rodrigues FF,Coimbra TM

    更新日期:2004-04-01 00:00:00

  • Need for tracheostomy after lung transplant predicts decreased mid- and long-term survival.

    abstract:BACKGROUND:Tracheostomy is an important adjunct for lung transplant patients requiring prolonged ventilation. We explored the effects of post-transplant tracheostomy on survival and bronchiolitis obliterans syndrome after lung transplant. METHODS:A retrospective, single center analysis was performed on all lung transp...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/ctr.13766

    authors: Huddleston SJ,Brown R,Rudser K,Goswami U,Tomic R,Lemke NT,Shaffer AW,Soule M,Hertz M,Shumway S,Kelly R,Loor G

    更新日期:2020-01-01 00:00:00

  • Successful transplantation of 50 single unit pediatric kidneys ages 11 to 48 months into adult recipients.

    abstract::There is currently an imbalance between the need for cadaveric kidneys for transplantation and the supply. The medical criteria for accepting cadaveric donors are changing and organs that were originally thought to be unacceptable have functioned well. Previous reports have discussed the problems with transplanting ...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:

    authors: Jacoby K,Lippman HR,Hura CE,McFarlin L,Jones CF,Banowsky LH

    更新日期:1992-12-01 00:00:00

  • Quality of life assessment in renal transplant: review and future directions.

    abstract::We performed a systematic search of the literature using the MEDLINE (through 2006) database to identify the patient-reported outcome measures of quality of life (QOL) used most often in the renal transplant literature. After applying limits and reviewing references, we identified 338 renal transplant articles with a ...

    journal_title:Clinical transplantation

    pub_type: 杂志文章,评审

    doi:10.1111/j.1399-0012.2007.00784.x

    authors: Butt Z,Yount SE,Caicedo JC,Abecassis MM,Cella D

    更新日期:2008-05-01 00:00:00

  • Does comfort therapy during controlled donation after circulatory death shorten the life of potential donors?

    abstract:INTRODUCTION:Controlled donation after circulatory death (DCD) remains ethically controversial. The authors developed a controlled DCD protocol in which comfort therapy is regularly used. The aim of this study was to determine whether this policy shortens the DCD donors' life. METHODS:The authors retrospectively analy...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/ctr.12276

    authors: Ledoux D,Delbouille MH,Deroover A,Lambermont B,Meurisse M,Damas P,Joris J,Detry O

    更新日期:2014-01-01 00:00:00

  • Organ donation in Hawaii: impact of the final rule.

    abstract:BACKGROUND:In an effort to increase organ donation, the Department of Health and Human Services issued the Final Rule in 1998. The Health Care Financing Administration (HCFA) later required hospitals to notify organ procurement organizations (OPO) of all deaths and imminent deaths in order to remain eligible for Medica...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1034/j.1399-0012.2002.01102.x

    authors: Higashigawa KH,Carroll C,Wong LL,Wong LM

    更新日期:2002-06-01 00:00:00

  • High-dose busulfan and the risk of pulmonary mortality after autologous stem cell transplant.

    abstract::The non-relapse mortality of autologous stem cell transplant is low enough that the procedure has been extended to older patients with non-Hodgkin's lymphoma. We treated 537 non-Hodgkin's lymphoma patients with high-dose chemotherapy consisting of busulfan, cyclophosphamide, and etoposide followed by autologous stem c...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2006.00581.x

    authors: Kalaycio M,Pohlman B,Kuczkowski E,Rybicki L,Andresen S,Sobecks R,Bolwell B

    更新日期:2006-11-01 00:00:00

  • Mycobacterium tuberculosis infections in solid organ transplantation: Guidelines from the infectious diseases community of practice of the American Society of Transplantation.

    abstract::These updated guidelines from the Infectious Diseases Community of Practice of the American Society of Transplantation review the diagnosis, prevention, and management of tuberculosis in the pre- and post-transplant period. The challenges of screening for both latent and active TB in the setting of transplantation are...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/ctr.13513

    authors: Subramanian AK,Theodoropoulos NM,Infectious Diseases Community of Practice of the American Society of Transplantation.

    更新日期:2019-09-01 00:00:00

  • A comparison of daclizumab to ATGAM induction in simultaneous pancreas-kidney transplant recipients on triple maintenance immunosuppression.

    abstract::Daclizumab (DAC) is a molecularly engineered humanized IgGa monoclonal Ab directed against the alpha chain of the interleukin-2 receptor (IL2R). Inhibiting the amplification of the immune response by blocking IL2R can reduce the frequency of acute rejection without the attendant risk of infection. The purpose of this ...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1034/j.1399-0012.2000.14040902.x

    authors: Rasaiah SB,Light JA,Sasaki TM,Currier CB

    更新日期:2000-08-01 00:00:00

  • Current principles and practice in autologous intraportal islet transplantation: a meta-analysis of the technical considerations.

    abstract:OBJECTIVES:Autologous islet transplantation (IAT) following pancreatectomy is now a recognized, albeit highly specialized procedure carried out in a small number of centers worldwide. Current clinical principles and best practice with emphasis on examining the technical aspects of surgery in centers with significant IA...

    journal_title:Clinical transplantation

    pub_type: 杂志文章,meta分析,评审

    doi:10.1111/ctr.12695

    authors: Kumar R,Chung WY,Dennison AR,Garcea G

    更新日期:2016-04-01 00:00:00

  • Older living donors provide excellent quality kidneys: a single center experience (older living donors).

    abstract:BACKGROUND:The role of advanced age live donors remains controversial because of decline in glomerular filtration rate and perceived increased risks of perioperative complications. METHODS:A retrospective review of all live donor transplants performed from January 2000 to December 2003. RESULTS:Seventy-eight live don...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2004.00328.x

    authors: Johnson SR,Khwaja K,Pavlakis M,Monaco AP,Hanto DW

    更新日期:2005-10-01 00:00:00

  • Orthotopic liver transplantation for adults with Alagille syndrome.

    abstract:INTRODUCTION:Alagille syndrome (AGS) is an inherited multisystem disorder, and liver transplantation (LT) may be required in pediatric patients with AGS (P-AGS). There are limited data regarding the outcomes of LT in adults with AGS (A-AGS). AIM:To determine and compare the outcomes of LT in A-AGS vs. P-AGS as well as...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2011.01574.x

    authors: Arnon R,Annunziato R,Schiano T,Miloh T,Baisley M,Sogawa H,Contreras AG,Lee S,Kerkar N

    更新日期:2012-03-01 00:00:00

  • Two-stage liver transplantation: an effective procedure in urgent conditions.

    abstract::Temporary portocaval shunt and total hepatectomy is a technique used in the presence of toxic liver syndrome because of fulminant hepatic failure, hepatic trauma, primary non-function (PNF), and eclampsia. We performed this technique on four patients. An indication for anhepatic state was severe hemodynamic instabilit...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2009.01118.x

    authors: Montalti R,Busani S,Masetti M,Girardis M,Di Benedetto F,Begliomini B,Rompianesi G,Rinaldi L,Ballarin R,Pasetto A,Gerunda GE

    更新日期:2010-01-01 00:00:00

  • Reviews on mysterious vascular endothelial cells in renal allografts.

    abstract::Endothelial cells (ECs) are involved in allograft rejection and are prime targets, but also key players. HLA antigens are the most prominent targets of alloantibodies in transplantation. Alloantibodies against other antigens such as ABO blood group antigens and non-HLA antigens could also be demonstrated. Alloantibodi...

    journal_title:Clinical transplantation

    pub_type: 杂志文章,评审

    doi:10.1111/j.1399-0012.2012.01675.x

    authors: Yamaguchi Y

    更新日期:2012-07-01 00:00:00

  • Peripheral endothelial dysfunction in heart transplant recipients: possible role of proinflammatory cytokines.

    abstract::Endothelium-dependent vasodilation in the peripheral circulation may be impaired in heart transplant recipients (HTx rec). Conflicting results have been obtained and the mechanisms involved have not been examined. In the present study, we examined whether long-time survivors of heart transplantation (Tx) show signs of...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1034/j.1399-0012.2000.140307.x

    authors: Holm T,Aukrust P,Andreassen AK,Ueland T,Brosstad F,Frøland SS,Simonsen S,Gullestad L

    更新日期:2000-06-01 00:00:00

  • Growth of a nation part I: impact of organ donor obesity on whole-organ pancreas transplantation.

    abstract::Obesity has reached epidemic proportions in the USA. Consequently, there are an increasing number of potential organ donors that are obese, but would otherwise be appropriate donors for pancreas transplantation (PTx). This is a retrospective study of all PTx performed at Indiana University between 2003 and 2009 (n = 3...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2011.01419.x

    authors: Fridell JA,Mangus RS,Taber TE,Goble ML,Milgrom ML,Good J,Vetor R,Powelson JA

    更新日期:2011-05-01 00:00:00

  • Tuberculosis in southern Chinese renal-transplant recipients.

    abstract:OBJECTIVES:To analyze the characteristics of tuberculosis (TB) in Southern Chinese renal transplant recipients, and summarize the corresponding experiences in diagnosis and management. METHOD:Retrospectively study 41 documented post-transplant TB cases out of the 2333 patients who received kidney transplantation in th...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2008.00878.x

    authors: Chen SY,Wang CX,Chen LZ,Fei JG,Deng SX,Qiu J,Li J,Chen GO,Fu HM,Xie CM

    更新日期:2008-11-01 00:00:00

  • CXCL10 levels identify individuals with rapid fibrosis at 12 months post-transplant for hepatitis C virus and predict treatment response.

    abstract:BACKGROUNDS:Recurrent hepatitis C virus (HCV) infection is universal post-transplantation. Fibrosis (F) stage ≥2 at 12 months identifies patients with rapid fibrosis progression. Antiviral therapy (AVT) remains the only option to attenuate fibrosis progression. We hypothesized that CXCL10 levels can distinguish between...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/ctr.12354

    authors: Joshi D,Carey I,Foxton M,Al-Freah M,Bruce M,Heaton N,Quaglia A,O'Grady J,Aluvihare V,Agarwal K

    更新日期:2014-05-01 00:00:00

  • Clinical relevance of endoscopy with histopathological assessment in children with suspected gastrointestinal graft-versus-host disease.

    abstract::Endoscopy with histopathological assessment is an established practice to confirm gastrointestinal graft-versus-host disease (GI-GVHD). However, the clinical relevance of this approach in children is incompletely evaluated. In a retrospective cohort study, we investigated the frequency of treatment changes in response...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/ctr.13867

    authors: Mårtensson T,Mellgren K,Toporski J,Arvidson J,Szakos A,Casswall TH,Gustafsson B

    更新日期:2020-07-01 00:00:00

  • Surgical strategies for liver transplantation in the case of portal vein thrombosis--current role of cavoportal hemitransposition and renoportal anastomosis.

    abstract::Portal vein thrombosis (PVT), a common complication of end stage liver disease, is no longer considered a definite contraindication for liver transplantation (LTx). The clinical decision to perform an LTx in the case of PVT depends on the degree of PVT and the experience of the surgeon. Eversion thromboendovenectomy w...

    journal_title:Clinical transplantation

    pub_type: 杂志文章,评审

    doi:10.1111/j.1399-0012.2006.00560.x

    authors: Paskonis M,Jurgaitis J,Mehrabi A,Kashfi A,Fonouni H,Strupas K,Büchler MW,Kraus TW

    更新日期:2006-09-01 00:00:00

  • Limitations of pediatric donor kidneys for transplantation.

    abstract:BACKGROUND:The shortage of grafts for kidney transplantation (Tx) has resulted in an expansion of criteria for pediatric donors. However, the limitations of pediatric donors for kidney Tx are not yet clearly established. We investigated the outcomes of recipients with pediatric kidney grafts from Colorado to elucidate ...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:

    authors: Yagisawa T,Kam I,Chan L,Springer JW,Dunn S

    更新日期:1998-12-01 00:00:00

  • The complication rate of intravascular ultrasound (IVUS) in a multicenter pediatric heart transplant population: A study of the international pediatric IVUS consortium.

    abstract:BACKGROUND:Our purpose was to determine the complication rate from intravascular ultrasound (IVUS) in a large, multicenter cohort of pediatric heart transplant (PHT) patients. METHODS:We retrospectively reviewed all PHT who underwent IVUS at 5 institutions (2006-2014). Rates of major and minor complications were calcu...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/ctr.13981

    authors: Auerbach SR,Fenton MJ,Grutter G,Albert DC,Di-Filippo S,Burch M,Kuhn MA

    更新日期:2020-09-01 00:00:00

  • Direct pressure measurement in the hepatic artery during liver transplantation: can it prevent the "steal" syndrome?

    abstract::Splenic artery "steal" syndrome after orthotopic liver transplantation (OLT) is an important cause of graft dysfunction. Direct pressure measurement in the hepatic (HA) and radial artery (RA) may identify patients at risk allowing its prevention. This observational study compared radial and hepatic mean arterial press...

    journal_title:Clinical transplantation

    pub_type: 杂志文章

    doi:10.1111/j.1399-0012.2011.01478.x

    authors: Wojcicki M,Pakosz-Golanowska M,Lubikowski J,Post M,Jarosz K,Milkiewicz P

    更新日期:2012-03-01 00:00:00

  • Liver cell transplantation in children.

    abstract::With increasing shortage of donor organs and changes in organ allocation systems, there is a considerable need for alternative or supportive techniques in the field of liver transplantation. Liver cell transplantation (LCT) is such an innovative technique that is especially promising for use in children, because it is...

    journal_title:Clinical transplantation

    pub_type: 杂志文章,评审

    doi:10.1111/j.1399-0012.2009.01113.x

    authors: Meyburg J,Schmidt J,Hoffmann GF

    更新日期:2009-12-01 00:00:00