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Novel methods for the molecular discrimination of Fasciola spp. on the basis of nuclear protein-coding genes.

Abstract：

:Fasciolosis is an economically important disease of livestock caused by Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola flukes. The aspermic Fasciola flukes have been discriminated morphologically from the two other species by the absence of sperm in their seminal vesicles. To date, the molecular discrimination of F. hepatica and F. gigantica has relied on the nucleotide sequences of the internal transcribed spacer 1 (ITS1) region. However, ITS1 genotypes of aspermic Fasciola flukes cannot be clearly differentiated from those of F. hepatica and F. gigantica. Therefore, more precise and robust methods are required to discriminate Fasciola spp. In this study, we developed PCR restriction fragment length polymorphism and multiplex PCR methods to discriminate F. hepatica, F. gigantica, and aspermic Fasciola flukes on the basis of the nuclear protein-coding genes, phosphoenolpyruvate carboxykinase and DNA polymerase delta, which are single locus genes in most eukaryotes. All aspermic Fasciola flukes used in this study had mixed fragment pattern of F. hepatica and F. gigantica for both of these genes, suggesting that the flukes are descended through hybridization between the two species. These molecular methods will facilitate the identification of F. hepatica, F. gigantica, and aspermic Fasciola flukes, and will also prove useful in etiological studies of fasciolosis.

journal_name

Parasitol Int

journal_title

Parasitology international

authors

Shoriki T,Ichikawa-Seki M,Suganuma K,Naito I,Hayashi K,Nakao M,Aita J,Mohanta UK,Inoue N,Murakami K,Itagaki T

doi

10.1016/j.parint.2015.12.002

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

180-3

issue

3

eissn

1383-5769

issn

1873-0329

pii

S1383-5769(15)00197-X

journal_volume

65

pub_type

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