Abstract:
:Hydroethidine has been used as a viability dye in various haemoparasites, including Plasmodium species. We compared flow cytometric quantification by hydroethidine and thiazole orange in P. falciparum. Dead parasites that did not develop or replicate exhibited high levels of DNA fragmentation and abnormal microscopic morphology, but were detected as viable ring-stage parasites by hydroethidine. Hydroethidine quantification was similar to thiazole orange, a DNA-binding dye that stains live and dead parasites. Data obtained cast concerns on hydroethidine as a suitable viability dye in P. falciparum and highlight the necessity of proper gating in flow cytometric studies quantifying parasitaemia.
journal_name
Parasitol Intjournal_title
Parasitology internationalauthors
Engelbrecht D,Coetzer TLdoi
10.1016/j.parint.2012.07.002subject
Has Abstractpub_date
2012-12-01 00:00:00pages
731-4issue
4eissn
1383-5769issn
1873-0329pii
S1383-5769(12)00095-5journal_volume
61pub_type
杂志文章abstract::The clinical features of neurocysticercosis (NCC) largely depend on the number, type, size, localization and stage of development of cysticerci, as well as on the host immune response against the parasite. Seizures are widely reported to be the most common symptom, occurring in 70-90% of patients, while NCC is conside...
journal_title:Parasitology international
pub_type: 杂志文章,评审
doi:10.1016/j.parint.2005.11.016
更新日期:2006-01-01 00:00:00
abstract::Visceral pentastomiasis (porocephalosis) caused by Armillifer armillatus larvae was incidentally diagnosed in a female striped hyena (Hyaena hyaena) of unknown age which died unexpectedly in 2013. The hyena had been imported from Tanzania 8years earlier and have been since then in a zoo in Chiang Mai, northern Thailan...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2015.10.004
更新日期:2016-02-01 00:00:00
abstract::Leishmaniasis is one of the major infectious diseases affecting the poorest regions of the world. The present study evaluated the antileishmanial activity of a guaianolide purified from the hydroalcoholic extract of aerial parts of Tanacetum parthenium (L.) Schultz Bip. The isolated compound showed activity against th...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2010.08.005
更新日期:2010-12-01 00:00:00
abstract::Diphyllobothrium hottai Yazaki, Fukumoto & Abe, 1988 was described based on the morphology of adult worms recovered from golden hamsters that had been experimentally infected with plerocercoids obtained from Japanese surf smelts (Hypomesus pretiosus japonicus) and olive rainbow smelts (Osmerus eperlanus mordax). Altho...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2016.06.011
更新日期:2016-10-01 00:00:00
abstract::Three species of the genus Allocreadium were collected from cyprinid fish in the territory of the Russian Far East: Allocreadium khankaiensis sp.nov; Allocreadium hemibarbi Roitman, 1963, and Allocreadium sp. 1. It is established that, according to morphometric values, the species A. khankaiensis sp.nov. has high morp...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2020.102102
更新日期:2020-06-01 00:00:00
abstract::Brasicystis bennetti Thatcher, 1979 was first described from specimens obtained from the subcutaneous tissues of the mouth and operculum of Plagioscyion squamosissimus from the Amazon River in Brazil, however since 2008, Brasicystis has been considered a genus inquirendum. This study reviews some of the diagnostic cha...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2013.01.001
更新日期:2013-04-01 00:00:00
abstract::Brazilian sardine is emerging as a promising species in Aquaculture. This study describes for the first time a case of parasitic infestation by Amyloodinium in Brazilian sardines Sardinella brasiliensis obtained from natural spawning in captivity. The sardines kept in nurseries were naturally parasitized by the amylod...
journal_title:Parasitology international
pub_type:
doi:10.1016/j.parint.2020.102091
更新日期:2020-06-01 00:00:00
abstract::The role of neutrophils in mediating host inflammation was examined in mice vaccinated with living third-stage infective hookworm larvae (L3). Mice were vaccinated by oral immunization with 500 L3 (Ancylostoma caninum) once every 2 weeks for a total of three immunizations. The vaccinated mice were then challenged intr...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/s1383-5769(01)00081-2
更新日期:2001-11-01 00:00:00
abstract::Schistosomiasis is endemic in Indonesia in two isolated areas, Lindu valley and Napu valley, both located in the Province of Central Sulawesi. In 1940, a prevalence survey was initiated in Lake Lindu, which indicated a Schistosoma japonicum infection prevalence of 56% among the population of Anca, Tomado and Langko vi...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2008.04.008
更新日期:2008-09-01 00:00:00
abstract::The third-stage (L3) larvae of Anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into Anisakis Type I larvae and Anisakis Type II larvae. Genetic analysis has allowed easy identification of these larvae: Anisakis Type I larvae include the species Anisakis simplex se...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2011.02.008
更新日期:2011-06-01 00:00:00
abstract::We report here the molecular characterization and possible function of a cysteine protease (termed HlCPL-A) identified in the midgut of the hard tick Haemaphysalis longicornis. HlCPL-A is a 333 amino acid protein belonging to the papain family of the cysteine protease. A construct encoding proHlCPL-A was expressed in ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2009.05.003
更新日期:2009-09-01 00:00:00
abstract::Granuloma modulation induced by antigen is an attractive model for vaccination studies of experimental schistosomiasis to test the effect of anti-pathology vaccine. We describe here an immunization procedure with culture derived macrophages-pulsed PIII, a known anionic antigen purified from S. mansoni adult worm, invo...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/s1383-5769(02)00031-4
更新日期:2002-09-01 00:00:00
abstract::In this study, we genetically characterised Monogenea found on common carp in Iran. In total, 5603 parasites were recovered from 112 fish. The parasites were first identified morphologically as Dactylogyrus extensus, D. anchoratus, D. vastator, D. minutus, D. achmerowi and Gyrodactylus sprostonae. Representative sampl...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2019.101977
更新日期:2019-12-01 00:00:00
abstract::A cat excreting Echinococcus multilocularis eggs was recently identified in Hokkaido, representing the first such observation in Japan. The cat was raised free-range and frequently ate rodents. Fecal egg examination revealed eggs of taeniids (EPG: 440) and Spirometra spp. (EPG: >1000). PCR targeting part of the mitoch...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2008.07.001
更新日期:2008-12-01 00:00:00
abstract::Serum samples from 2970 (1400 sows, 1570 fattening) pigs, from 100 farms in the 10 main swine production regions in Spain were tested for antibodies against T. gondii by the modified agglutination test (MAT). Antibodies to T. gondii (MAT 1:25 or higher) were detected in 492 pigs (16.6%, 9.7% in fattening pigs and 24.2...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2010.06.001
更新日期:2010-09-01 00:00:00
abstract::Canine hepatozoonosis caused by Hepatozoon canis is a worldwide distributed tick-borne disease of domestic and wild canids that is transmitted by ingestion of Rhipicephalus sanguineus sensu lato (s.l.) ticks. The present study was aimed to determine the prevalence of Hepatozoon infections in 80 stray dogs from Havana ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2020.102200
更新日期:2021-02-01 00:00:00
abstract::Dientamoeba fragilis is an intestinal protozoan, an inhabitant of the human gastrointestinal tract, with a worldwide distribution. The reported prevalence of D. fragilis varies worldwide in different populations between 0.3% and 82.9%, and its role as a pathogen is still unclear. The parasite has been identified in th...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2020.102227
更新日期:2021-02-01 00:00:00
abstract::Neospora caninum is an intracellular protozoan that infects many domestic and wild animals. Domestic dogs and other canids function as definitive hosts, while other mammals serve as natural intermediate hosts. In the present study, the brain tissues of bats collected in Yunnan Province, Southern China were tested by N...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2018.03.002
更新日期:2018-08-01 00:00:00
abstract::The aim of the present study was to examine the frequency and genetic diversity of Blastocystis in cancer patients of a Medical Oncology Department in Aydın, Turkey. Patients' stool samples were examined between January 2013 and February 2014 by both microscopy and culture methods. Culture positive samples were subjec...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2016.02.010
更新日期:2016-12-01 00:00:00
abstract::We have previously reported a significant decrease in serum PON1 activity after Nippostrongylus brasiliensis infection in Wistar rats in association with the inflammatory response mounted against the parasite in the migratory phase of infection. However, the roles of intestinal phase and the associated oxidative stres...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2009.03.001
更新日期:2009-06-01 00:00:00
abstract::The studies on Cryptosporidium infections of animals in Turkey mostly rely on microscopic observation. Few data are available regarding the prevalence of Cryptosporidium genotypes and subtypes infection. The aim of this study is to analyse the detection of Cryptosporidium genotypes and subtypes from young ruminants. A...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2020.102163
更新日期:2020-12-01 00:00:00
abstract::Dioctophymosis is the disease caused by the nematode Dioctophyme renale, normally found parasitizing the right kidney of dogs. The absence of symptoms is frequent in parasitized animals. The surgical procedures are commonly performed to treat this disease. This work describes a case involving a canine with renal and e...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2020.102211
更新日期:2021-02-01 00:00:00
abstract::The objective of this study was to determine the level of resistance of Haemonchus contortus and Trichostrongylus colubriformis in sheep to levamisole, albendazole, ivermectin, moxidectin, closantel and trichlorfon. The parasites were isolated from sheep naturally infected by gastrointestinal nematodes and were then k...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2010.09.006
更新日期:2010-12-01 00:00:00
abstract::Trophozoites of the free-living amoeba, Naegleria fowleri, were isolated from the cerebrospinal fluid of meningoencephalitis patient. The infecting agent was identified as N. fowleri based on morphologic, serologic and molecular techniques carried out on the isolated organisms. ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2005.06.001
更新日期:2005-12-01 00:00:00
abstract::This report describes a rare case of ophthalmic dirofilariasis in a 2-year-old boy with redness, irritation, pain and foreign body sensation in the right eye. Slit lamp examination demonstrated a thread-like whitish nematode in the anterior chamber of the right eye that twisted around itself. The nematode worm (35mm l...
journal_title:Parasitology international
pub_type: 杂志文章,评审
doi:10.1016/j.parint.2016.10.022
更新日期:2017-02-01 00:00:00
abstract::European liver fluke (Opisthorchis felineus) and Asian liver fluke (Opisthorchis viverrini) are similar in morphology but comparative pathology of the infections has not been described. We therefore did comparative histopathology of both parasites in an experimental animal model. The study was conducted in 3 groups of...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2011.08.005
更新日期:2012-03-01 00:00:00
abstract::This study was conducted both to assess the prevalence of parasitic infections in two neighboring villages of Manisa province in Turkey, with different socioeconomic levels and investigate the influences of some individual and environmental factors on these infections. A total of 100 school children were enrolled and ...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2007.01.005
更新日期:2007-06-01 00:00:00
abstract::Leishmaniases are a complex of sand fly-borne diseases that are considered a public health issue in several countries. Brazil presents high leishmaniases rates. The South of Ceará State, known as Cariri region, shows worrying statistics mainly on American tegumentary leishmaniasis. In Barbalha, which is one of the mun...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2020.102245
更新日期:2021-02-01 00:00:00
abstract::A survey was carried out to investigate the occurrence of Sarcocystis infection in the loin (Musculus longissimus) of Japanese and imported beef. In all, the muscle tissue of 482 samples were examined by histological method. The prevalence of Sarcocystis unspecified species cysts was lower in Japanese beef (total 6.31...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/s1383-5769(98)00041-5
更新日期:1999-03-01 00:00:00
abstract::A series of sixteen benzoylthioureas derivatives were initially evaluated in vitro against the epimastigote form of Trypanosoma cruzi. All of the tested compounds inhibited the growth of this form of the parasite, and due to the promising anti-epimastigote activity from three of these compounds, they were also assayed...
journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2020.102225
更新日期:2021-02-01 00:00:00