The walking dead: is hydroethidine a suitable viability dye for intra-erythrocytic Plasmodium falciparum?

Abstract:

:Hydroethidine has been used as a viability dye in various haemoparasites, including Plasmodium species. We compared flow cytometric quantification by hydroethidine and thiazole orange in P. falciparum. Dead parasites that did not develop or replicate exhibited high levels of DNA fragmentation and abnormal microscopic morphology, but were detected as viable ring-stage parasites by hydroethidine. Hydroethidine quantification was similar to thiazole orange, a DNA-binding dye that stains live and dead parasites. Data obtained cast concerns on hydroethidine as a suitable viability dye in P. falciparum and highlight the necessity of proper gating in flow cytometric studies quantifying parasitaemia.

journal_name

Parasitol Int

authors

Engelbrecht D,Coetzer TL

doi

10.1016/j.parint.2012.07.002

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

731-4

issue

4

eissn

1383-5769

issn

1873-0329

pii

S1383-5769(12)00095-5

journal_volume

61

pub_type

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