Abstract:
:Cellular proliferation, differentiation, and morphogenesis are shaped by multiple signaling cascades, and their dysregulation plays an integral role in cancer progression. Three cascades that contribute to oncogenic potential are those mediated by Wnt proteins and the receptor Frizzled (FZD), growth factor receptor tyrosine kinases (RTKs), and heterotrimeric G proteins and associated GPCRs. Daple is a guanine nucleotide exchange factor (GEF) for the G protein Gαi Daple also binds to FZD and the Wnt/FZD mediator Dishevelled (Dvl), and it enhances β-catenin-independent Wnt signaling in response to Wnt5a-FZD7 signaling. We identified Daple as a substrate of multiple RTKs and non-RTKs and, hence, as a point of convergence for the three cascades. We found that phosphorylation near the Dvl-binding motif in Daple by both RTKs and non-RTKs caused Daple/Dvl complex dissociation and augmented the ability of Daple to bind to and activate Gαi, which potentiated β-catenin-independent Wnt signals and stimulated epithelial-mesenchymal transition (EMT) similarly to Wnt5a/FZD7 signaling. Although Daple acts as a tumor suppressor in the healthy colon, the concurrent increased abundance of Daple and epidermal growth factor receptor (EGFR) in colorectal tumors was associated with poor patient prognosis. Thus, the Daple-dependent activation of Gαi and the Daple-dependent enhancement of β-catenin-independent Wnt signals are not only stimulated by Wnt5a/FZD7 to suppress tumorigenesis but also hijacked by growth factor-activated RTKs to enhance tumor progression. These findings identify a cross-talk paradigm among growth factor RTKs, heterotrimeric G proteins, and the Wnt/FZD pathway in cancer.
journal_name
Sci Signaljournal_title
Science signalingauthors
Aznar N,Ear J,Dunkel Y,Sun N,Satterfield K,He F,Kalogriopoulos NA,Lopez-Sanchez I,Ghassemian M,Sahoo D,Kufareva I,Ghosh Pdoi
10.1126/scisignal.aao4220subject
Has Abstractpub_date
2018-02-27 00:00:00issue
519eissn
1945-0877issn
1937-9145pii
11/519/eaao4220journal_volume
11pub_type
杂志文章abstract::The prevalence, presentation, and progression of Alzheimer's disease (AD) differ between men and women, although β-amyloid (Aβ) deposition is a pathological hallmark of AD in both sexes. Aβ-induced activation of the neuronal glutamate receptor mGluR5 is linked to AD progression. However, we found that mGluR5 exhibits ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.abd2494
更新日期:2020-12-15 00:00:00
abstract::In this issue of Science Signaling, Lu et al reveal a role for Parkin-mediated mitophagy in beige-to-white adipocyte transition. In the absence of Parkin, mitochondria in thermogenic beige adipocytes are preserved even after the elimination of cold mimetic stimuli-in contrast to their typical elimination during the wh...
journal_title:Science signaling
pub_type: 评论,杂志文章,评审
doi:10.1126/scisignal.aat1082
更新日期:2018-04-24 00:00:00
abstract::The protein kinase ATM is a master regulator of the DNA damage response but also responds directly to oxidative stress. Loss of ATM causes ataxia telangiectasia, a neurodegenerative disorder with pleiotropic symptoms that include cerebellar dysfunction, cancer, diabetes, and premature aging. We genetically separated t...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aan5598
更新日期:2018-01-09 00:00:00
abstract::The prefibrillar form of soluble amyloid-β (sAβ1-42) impairs synaptic function and is associated with the early phase of Alzheimer's disease (AD). We investigated how sAβ1-42 led to presynaptic defects using a quantum dot-based, single particle-tracking method to monitor synaptic vesicle (SV) trafficking along axons. ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aam8661
更新日期:2017-07-11 00:00:00
abstract::This week, Science Signaling highlights research that uses computational and cell biology approaches to uncover the molecular mechanisms underlying diseases of the central nervous system. In particular, research featured explores the neuronal response to stress due to excess excitatory stimuli, multiple roles for pres...
journal_title:Science signaling
pub_type: 杂志文章,评审
doi:10.1126/scisignal.2004456
更新日期:2013-07-09 00:00:00
abstract::Insulin secretion from pancreatic β cells is controlled by nutrients, hormones, and neurotransmitters. Unlike the latter, which work through classic receptors, glucose and most other nutrients do not interact with membrane receptors but must be metabolized by β cells to induce insulin secretion. Studies have revealed ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2003325
更新日期:2012-08-28 00:00:00
abstract::Educated natural killer (NK) cells have inhibitory receptors specific for self major histocompatibility complex (MHC) class I molecules and kill cancer cells more efficiently than do NK cells that do not have such receptors (hyporesponsive NK cells). The mechanism behind this functional empowerment through education h...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aai9200
更新日期:2018-02-13 00:00:00
abstract::The transcription factor FOXP1 (forkhead box protein P1) is a master regulator of stem and progenitor cell biology. In diffuse large B cell lymphoma (DLBCL), copy number amplifications and chromosomal translocations result in overexpression of FOXP1. Increased abundance of FOXP1 in DLBCL is a predictor of poor prognos...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2005654
更新日期:2015-02-03 00:00:00
abstract::The TRAF [tumor necrosis factor receptor-associated factor] family of cytoplasmic adaptor proteins link cell-surface receptors to intracellular signaling pathways that regulate innate and adaptive immune responses. In response to activation of RIG-I (retinoic acid-inducible gene I), a component of a pattern recognitio...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2003152
更新日期:2012-11-13 00:00:00
abstract::Exosomes are paracrine regulators of the tumor microenvironment and contain complex cargo. We previously reported that exosomes released from acute myeloid leukemia (AML) cells can suppress residual hematopoietic stem and progenitor cell (HSPC) function indirectly through stromal reprogramming of niche retention facto...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aaf2797
更新日期:2016-09-06 00:00:00
abstract::Postsynaptic organizational protein complexes play central roles both in orchestrating synapse formation and in defining the functional properties of synaptic transmission that together shape the flow of information through neuronal networks. A key component of these organizational protein complexes is the family of s...
journal_title:Science signaling
pub_type: 杂志文章,评审
doi:10.1126/scisignal.abd8379
更新日期:2020-12-22 00:00:00
abstract::This two-issue series focuses on how understanding how signaling pathways are altered in cancer cells can enable researchers to develop more effective therapies. Cancer is a pleiotropic disease. Tumor cells change over time and, even within a specific tumor, cells may have different mutations and dependencies on diffe...
journal_title:Science signaling
pub_type: 社论
doi:10.1126/scisignal.2004149
更新日期:2013-03-26 00:00:00
abstract::Manipulating ligand interactions with the activating receptor NKG2D modulates natural killer cell responses. ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aan8369
更新日期:2017-05-30 00:00:00
abstract::Fragile X syndrome (FXS) mental retardation is caused by loss-of-function mutations in an RNA-binding protein, fragile X mental retardation protein (FMRP). Previous studies in patients or animal models of FXS have identified alterations in dendritic spine structure, as well as synaptic plasticity induced by metabotrop...
journal_title:Science signaling
pub_type: 杂志文章,评审
doi:10.1126/stke.15pe6
更新日期:2008-02-05 00:00:00
abstract::The CXC chemokine CXCL12 mediates the chemoattraction of T cells and enhances the stimulation of T cells through the T cell receptor (TCR). The adaptor SLP-76 [Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD] has two key tyrosine residues, Tyr(113) and Tyr(128), that mediate signaling downstream of t...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2004018
更新日期:2013-07-30 00:00:00
abstract::A major endpoint of the canonical Wnt signaling pathway is a change in the transcription of target genes. The transcription factors lymphoid enhancer factor (LEF) and T cell factor (TCF) serve as the main gatekeepers of these changes by selecting genes to be targeted by the transcriptional coregulator beta-catenin and...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.139pe43
更新日期:2008-09-30 00:00:00
abstract::Insight into the signaling pathways that regulate de novo lipogenesis may lead to new treatments for hepatic steatosis. ...
journal_title:Science signaling
pub_type: 杂志文章,评审
doi:10.1126/scisignal.aam9988
更新日期:2017-02-21 00:00:00
abstract::In a mouse model of Parkinson's disease, new evidence shows that l-DOPA, which is used to treat the symptoms of the disease but also causes dyskinesia, results in a persistent activation of the protein kinase mTOR (mammalian target of rapamycin) in a subset of striatal medium spiny neurons. Moreover, blockade of a spe...
journal_title:Science signaling
pub_type: 杂志文章,评审
doi:10.1126/scisignal.280pe42
更新日期:2009-07-21 00:00:00
abstract::How cells recover from a DNA damage-induced arrest is currently poorly understood. We performed large-scale quantitative phosphoproteomics to identify changes in protein phosphorylation that occurred during recovery from arrest in the G2 phase of the cell cycle caused by DNA damage. We identified 154 proteins that wer...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2003664
更新日期:2013-04-23 00:00:00
abstract::Birth defects of the heart and face are common, and most have no known genetic cause, suggesting a role for environmental factors. Maternal fever during the first trimester is an environmental risk factor linked to these defects. Neural crest cells are precursor populations essential to the development of both at-risk...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aal4055
更新日期:2017-10-10 00:00:00
abstract::Skeletal muscle mass is regulated by activity, metabolism, and the availability of nutrients. During muscle atrophy, MNK2 expression increases. We found that MNK2 (mitogen-activated protein kinase-interacting kinase 2), but not MNK1, inhibited proteins involved in promoting protein synthesis, including eukaryotic tran...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2002466
更新日期:2012-02-14 00:00:00
abstract::The extracellular Ca(2+)-sensing receptor (CaSR) plays a nonredundant role in the functions of the parathyroid gland (PTG) and the kidney. Severe hyperparathyroidism, premature death, and incomplete gene excision in Casr(-/-) mice have precluded the assessment of CaSR function in other tissues. We generated mice with ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.1159945
更新日期:2008-09-02 00:00:00
abstract::Store-operated Ca2+ entry (SOCE) channels are highly selective Ca2+ channels activated by the endoplasmic reticulum (ER) sensors STIM1 and STIM2. Their direct interaction with the pore-forming plasma membrane ORAI proteins (ORAI1, ORAI2, and ORAI3) leads to sustained Ca2+ fluxes that are critical for many cellular fun...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aav4663
更新日期:2019-04-23 00:00:00
abstract::Fgr is a member of the Src family of nonreceptor tyrosine kinases, which are overexpressed and constitutively active in many human cancers. Fgr expression is restricted to myeloid hematopoietic cells and is markedly increased in a subset of bone marrow samples from patients with acute myeloid leukemia (AML). Here, we ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aat5916
更新日期:2018-10-23 00:00:00
abstract::The LKB1 serine-threonine kinase is a tumor suppressor that is inactivated in a large number of sporadic human lung non-small cell carcinomas (NSCLCs) and cervical cancers. Genetic deletion of LKB1 in various mouse tissues results in tumorigenesis, and loss of LKB1 increases metastasis in a mouse model of NSCLC. LKB1 ...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.286pe55
更新日期:2009-09-01 00:00:00
abstract::Degrons are the elements that are used by E3 ubiquitin ligases to target proteins for degradation. Most degrons are short linear motifs embedded within the sequences of modular proteins. As regulatory sites for protein abundance, they are important for many different cellular processes, such as progression through the...
journal_title:Science signaling
pub_type: 杂志文章,评审
doi:10.1126/scisignal.aak9982
更新日期:2017-03-14 00:00:00
abstract::Interleukin-10 (IL-10) is an important anti-inflammatory cytokine that is produced primarily by macrophages. We investigated mechanisms by which the timing of IL-10 production was controlled in macrophages and found that cyclin-dependent kinase 5 (CDK5) activity was markedly increased in lipopolysaccharide (LPS)-stimu...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aab3156
更新日期:2015-11-24 00:00:00
abstract::Regulatory factor X (Rfx) homologs regulate the transcription of genes necessary for ciliogenesis in invertebrates and vertebrates. Primary cilia are necessary for Hedgehog signaling and regulation of the activity of the transcriptional regulators known as Gli proteins, which are targets of Hedgehog signaling. Here, w...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2000602
更新日期:2009-11-03 00:00:00
abstract::The PAR-1-MARK pathway controls cell polarity through the phosphorylation of microtubule-associated proteins. Rho-Rac guanine nucleotide exchange factor 2 (ARHGEF2), which activates Ras homolog family member A (RHOA), is anchored to the microtubule network and sequestered in an inhibited state through binding to dynei...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.aan3286
更新日期:2017-10-31 00:00:00
abstract::TRPM2 is a Ca2+-permeable cation channel that is specifically activated by adenosine diphosphoribose (ADPR). Channel activation in the plasma membrane leads to Ca2+ influx and has been linked to apoptotic mechanisms. The primary agonist, ADPR, is produced both extra- and intracellularly and causes increases in intrace...
journal_title:Science signaling
pub_type: 杂志文章
doi:10.1126/scisignal.2000278
更新日期:2009-05-19 00:00:00