Abstract：
:Thaumatin-like proteins (TLPs) are the products of a large, highly complex gene family involved in host defense. TLPs also belong to the pathogenesis-related family 5 (PR-5) of plant defense proteins. Most TLPs exhibit potential antifungal activities, and their accumulation in the plant is related to many physiological processes. In this study, a gene encoding TLP named permatin with an open reading frame of 678 bp encoding a protein of 225 amino acids with a calculated molecular mass of 23.5 kDa was cloned from naked oat leaves. Phylogenetic analysis revealed that permatin shares high homology with a number of other TLPs among diverse taxa. Model of structure by homology modeling showed that permatin consists of an acidic cleft region consistent with most TLPs. Recombinant NusA-permatin was overexpressed in Escherichia coli strain BL21 and purified by Heparin column combined with Sephacryl S-200 column. The protein exhibited antifungal activity to Fusarium oxysporum (half maximal inhibitory concentration, IC50 = 21.42 μM). Morphological observation showed that NusA-permatin can induce mycelium deformation of F. oxysporum, the cell membrane is blurred, and the diaphragm is not obvious. NusA-permatin also causes membrane permeabilization and reactive oxygen species accumulation in the mycelium of F. oxysporum. Permatin may play an important role in the disease resistance responses of plants against pathogen attacks through its antifungal activity.
journal_name
Probiotics Antimicrob Proteinsjournal_title
Probiotics and antimicrobial proteinsauthors
Liu J,Han D,Shi Ydoi
10.1007/s12602-018-9422-ysubject
Has Abstractpub_date
2019-03-01 00:00:00pages
299-309issue
1eissn
1867-1306issn
1867-1314pii
10.1007/s12602-018-9422-yjournal_volume
11pub_type
杂志文章abstract：:It has been recently shown, that certain strains/isolates of Bacillus subtilis can be used as a probiotic for humans. The production of the macrocyclic sactibiotic subtilosin in B. subtilis ATCC 6633 is highly regulated. To improve the subtilosin productivity of B. subtilis, different growth conditions were compared f...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09547-4
更新日期：2020-06-01 00:00:00
abstract：:Information regarding cellular anti-senescence attributes of probiotic bacteria vis-à-vis modulation of senescence-associated secretory phenotype (SASP) and mTOR signaling is very limited. The present study assessed anti-senescence potential of secretory metabolites of probiotic Lactobacillus fermentum (Lact. fermentu...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09576-z
更新日期：2020-06-01 00:00:00
abstract：:Newly hatched broiler chickens are the most susceptible to Salmonella infections, especially during the first 24 h. At this age, the gut microbiome is not fully developed and offers little protection in the form of competitive exclusion. In this study, one group of newly hatched, Salmonella-free broilers were colonise...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09593-y
更新日期：2020-09-01 00:00:00
abstract：:The aim of this work is to characterize the potential probiotic properties of 14 antifungal Lactobacillus strains isolated from traditional fermenting Moroccan green olives. The molecular identification of strains indicated that they are composed of five Lactobacillus brevis, two Lactobacillus pentosus, and seven Lact...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09543-8
更新日期：2020-06-01 00:00:00
abstract：:The effect of two putative probiotic strains, Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74, on the control of cholesterol efflux in enterocytes was assessed by focusing on the promotion of ATP-binding cassette sub-family G members 5 and 8 (ABCG5 and ABCG8). Differentiated Caco-2 enterocytes were tr...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-011-9086-3
更新日期：2011-12-01 00:00:00
abstract：:It is well established that bacteria communicate between each other by using different mechanisms; among which, quorum sensing (QS) is the best known one. Indeed, intra- and intercellular communications of microorganisms, as well as the regulation of metabolism and reaction to the surrounding environmental conditions,...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章,评审
doi：10.1007/s12602-019-09555-4
更新日期：2020-03-01 00:00:00
abstract：:The study reports the growth, acidification and proteolysis of eight selected lactic acid bacteria in skim and soy milk. Angiotensin-converting enzyme inhibition and antimicrobial profiles of skim and soy milk fermented by the lactic acid bacteria were also determined. Among eight lactic cultures (S. thermophilus MD2,...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-017-9279-5
更新日期：2018-06-01 00:00:00
abstract：:Probiotics are a nutritional tool for disease prevention. It has been proposed that stimulation of immune response could affect the growth-promoting properties of antimicrobial growth promoters as well as the control of foodborne pathogens. The current study compares immune response in the blood of 280 non-infected an...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-010-9054-3
更新日期：2010-10-01 00:00:00
abstract：:The effects of treatment with probiotics on the immunological and hematobiochemical changes in Trypanosoma brucei infection were investigated. Probiotic strains used are Bifidobacterium BB-12, Lactobacillus acidophilus LA-5, Lactobacillus delbrueckii LBY-27, Lactobacillus paracasei LC-01, and Streptococcus thermophilu...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09592-z
更新日期：2020-09-01 00:00:00
abstract：:The neutral outcome of the recently reported school-based trial of probiotic K12 (The effect of the oral probiotic Streptococcus salivarius K12 on group A streptococcus pharyngitis: a pragmatic trial in schools) can be attributed at least partially to several readily identifiable confounding factors. Mainly, the execu...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-018-9398-7
更新日期：2019-03-01 00:00:00
abstract：:Microbial diseases in fish, plant, animal and human are rising constantly; thus, discovery of their antidote is imperative. The use of antibiotic in aquaculture further compounds the problem by development of resistance and consequent consumer health risk by bio-magnification. Antimicrobial peptides (AMPs) have been h...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-016-9215-0
更新日期：2016-09-01 00:00:00
abstract：:A total of 145 lactic acid bacteria isolated from a variety of Turkish red wines during malolactic fermentation were screened to find bacteriocin-producing strains. Among them, 14 isolates of Enterococcus faecium were identified to produce bacteriocins. PCR screening revealed that some isolates harbored entA and entB ...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-016-9222-1
更新日期：2016-09-01 00:00:00
abstract：:High-fat diet (HFD) consumption induces obesity and increases blood glucose, insulin resistance, and metabolic disorders. Recent studies suggest that probiotics might be a novel approach to counteract these effects in the treatment of obesity. Here, we evaluated the effect of Leuconostoc mesenteroides subsp. mesentero...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09556-3
更新日期：2020-06-01 00:00:00
abstract：:Three halotolerant lactobacilli (Lactobacillus plantarum, L. pentosus, and L. acidipiscis) isolated from a ripened Mexican tropical cheese (double cream Chiapas cheese) were evaluated as potential probiotics and compared with two commercial probiotic strains (L. casei Shirota and L. plantarum 299v) from human origin. ...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-013-9144-0
更新日期：2013-12-01 00:00:00
abstract：:Synbiotics are known to exert multiple beneficial effects, including anti-inflammatory and antioxidant actions. The aim of this study was to evaluate the effects of synbiotic supplementation on carotid intima-media thickness (CIMT), biomarkers of inflammation, and oxidative stress in people with overweight, diabetes, ...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章,随机对照试验
doi：10.1007/s12602-017-9343-1
更新日期：2019-03-01 00:00:00
abstract：:In the present study, the ability of bovine lactoferrin hydrolysate (LfH) to disrupt the cytoplasmic membrane of Escherichia coli O157:H7 was investigated. Lactoferrin and LfH antimicrobial activities were compared against E. coli O157:H7 and E. coli O157:H7 spheroplasts. The effect of LfH on the cytoplasmic membrane ...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-010-9039-2
更新日期：2010-06-01 00:00:00
abstract：:Food-grade yeasts make significant contributions to flavor development in fermented foods. Some yeast species also inhibit undesirable bacteria, yeasts and molds, apparently by producing antimicrobial compounds called mycocins. The aim of this study was to evaluate the ability of wild yeasts, isolated from raw milk an...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-012-9109-8
更新日期：2013-03-01 00:00:00
abstract：:Twenty-nine Lactobacillus plantarum strains isolated from different types of Polish regional cheeses (Oscypek and Korycinski) were assessed for selected probiotic properties and anti-staphylococcal activity. Most of the tested L. plantarum strains were considered safe. Whole bacterial cultures (WBC) and cell-free supe...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09587-w
更新日期：2020-09-01 00:00:00
abstract：:Dental diseases are among the common health issues experienced around the world. Dental caries is one of the most predominant oral diseases worldwide. Major factors associated with caries development include poor oral hygiene, the content of specific carbohydrates in the diet, dental biofilm formation, the cariogenic ...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章,评审
doi：10.1007/s12602-020-09652-9
更新日期：2020-12-01 00:00:00
abstract：:Lipopolysaccharide (LPS) is a toxic and immunogenic agent for human. Additionally, LPS is a good target for some antimicrobial compounds, including antimicrobial peptides (AMPs). LPS-binding peptides (LBPs) can recognize and neutralize LPS. Rabbit and human cathelicidins are AMPs with LPS-binding activity. In this stu...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-020-09648-5
更新日期：2020-12-01 00:00:00
abstract：:Ovarian cancer (OC) is a leading cause of death among women worldwide. Various evidences suggest that oncomiRs and Toll-like receptor 4 (TLR-4) signaling pathways appear to be key players in the initiation and progression of OC. It seems there exists a continuous intercommunication between cancer cells and normal micr...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09596-9
更新日期：2020-09-01 00:00:00
abstract：:In this work, we performed the rational design of a cationic antimicrobial peptide, GIBIMPY4, using the software DEPRAMPs developed at the GIBIM research group. GIBIMPY4 has a length of 17 amino acids, it is amphipathic, its structure is α-helix and it has a net charge of (+5). Solid-phase peptide synthesis was perfor...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-016-9219-9
更新日期：2016-09-01 00:00:00
abstract：:The present study was conducted to evaluate the effects of dietary supplementation of recombinant plectasin (Ple) on the growth performance, intestinal health, and serum immune parameters in broilers. A total of 288 1-day-old male broilers (Arbor Acres) were randomly allotted to four dietary treatments including the b...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-9515-2
更新日期：2020-03-01 00:00:00
abstract：:Urinary tract infections (UTIs) are the most common infectious diseases in infants and the elderly; they are also the most common among nosocomial infections. The treatment of UTIs usually involves a short-term course of antibiotics. The purpose of this study was to identify the strains of lactic acid bacteria (LAB) t...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-017-9302-x
更新日期：2018-06-01 00:00:00
abstract：:Probiotics offer numerous health benefits, including digestive and immune health. Improved digestive health is linked to a more efficient absorption of important nutrients from our diet. This review focused on the rationale of using the probiotic Bacillus coagulans GBI-30, 6086 to aid protein absorption and utilizatio...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章,评审
doi：10.1007/s12602-017-9354-y
更新日期：2018-12-01 00:00:00
abstract：:Probiotics have always been considered as a supplementary therapy for many diseases especially gut disorders. The absorption and barrier function of the gut play a vital role in the maintenance of body homeostasis. This study was to investigate the protective effects of Bacillus amyloliquefaciens SC06 (Ba) on H2O2-ind...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-019-09538-5
更新日期：2020-06-01 00:00:00
abstract：:IL-17A is a cytokine which is produced by several immune and non-immune cells. The cytokine plays dual roles from protection from microbes and protection from pro-inflammatory based diseases to induction of the pro-inflammatory based diseases. The main mechanisms which lead to the controversial roles of IL-17A are yet...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章,评审
doi：10.1007/s12602-017-9329-z
更新日期：2019-03-01 00:00:00
abstract：:Lactiplantibacillus plantarum subsp. plantarum 299v (L. plantarum 299v) is one of the most important probiotic strains in animal health, but the molecular mechanisms of how it exerts health benefits remain unclear. The purpose of this study was to explore the changes in miRNA expression profiles in the intestinal tiss...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-021-09743-1
更新日期：2021-01-24 00:00:00
abstract：:Axone (Akhuni) is a homemade synbiotic (Nagamese fermented soybean product) served as side dish in North Eastern India. In this study, effects of Axone feeding on growth, weight gain, sexual maturity and egg production on Vanaraja birds (a strain of poultry bird developed at PDP Hyderabad for villages and backyard pou...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-014-9160-8
更新日期：2014-06-01 00:00:00
abstract：:The purpose of this article is to reveal the role of the lactic acid bacteria (LAB) in the beebread transformation/preservation, biochemical properties of 25 honeybee endogenous LAB strains, particularly: antifungal, proteolytic, and amylolytic activities putatively expressed in the beebread environment have been stud...
journal_title：Probiotics and antimicrobial proteins
pub_type： 杂志文章
doi：10.1007/s12602-017-9379-2
更新日期：2018-12-01 00:00:00
