Distribution of Major Pilin Subunit Genes Among Atypical Enteropathogenic Escherichia coli and Influence of Growth Media on Expression of the ecp Operon.

Abstract:

:Atypical enteropathogenic Escherichia coli (aEPEC) strains are unable to produce the bundle-forming pilus (BFP), which is responsible for the localized adherence pattern, a characteristic of the pathogenicity of typical EPEC strains. The lack of BFP in aEPEC strains suggests that other fimbrial or non-fimbrial adhesins are involved in their adhesion to the host cells. The aim of this study was to investigate the distribution of major subunit fimbrial genes known to be important adherence factors produced by several E. coli pathotypes in a collection of 72 aEPEC strains. Our results demonstrate that a high percentage (94-100%) of aEPEC strains harbored ecpA, fimA, hcpA, and lpfA fimbrial genes. Other fimbrial genes including pilS, pilV, sfpA, daaC, papA, and sfa were detected at lower frequencies (1-8%). Genes encoding fimbrial subunits, which are characteristic of enteroaggregative E. coli or enterotoxigenic E. coli were not found. No correlation was found between fimbrial gene profiles and adherence phenotypes. Since all aEPEC strains contained ecpA, the major pilin gene of the E. coli common pilus (ECP), a subset of ecpA+ strains was analyzed for transcription of ecpRABCDE and production of ECP upon growth in three different culture conditions at 37°C. Transcription of ecpRABCDE occurred in all conditions; however, ECP production was medium dependent. In all, the data suggest that aEPEC strains are highly heterogeneous in terms of their fimbrial gene profiles. Despite lacking BFP production, other mechanisms of cell adherence exist in aEPEC strains to ensure host colonization, e.g., mediated by other prevalent pili such as ECP. Moreover, the production of ECP by aEPEC strains might be influenced by yet unknown post-transcriptional factors.

journal_name

Front Microbiol

authors

Munhoz DD,Nara JM,Freitas NC,Moraes CTP,Nunes KO,Yamamoto BB,Vasconcellos FM,Martínez-Laguna Y,Girón JA,Martins FH,Abe CM,Elias WP,Piazza RMF

doi

10.3389/fmicb.2018.00942

subject

Has Abstract

pub_date

2018-05-15 00:00:00

pages

942

issn

1664-302X

journal_volume

9

pub_type

杂志文章
  • Invasion of Nostocales (cyanobacteria) to Subtropical and Temperate Freshwater Lakes - Physiological, Regional, and Global Driving Forces.

    abstract::Similar to the increased number of studies on invasive plants and animals in terrestrial and aquatic ecosystems, many reports were recently published on the invasion of Nostocales (cyanobacteria) to freshwater environments worldwide. Invasion and proliferation of Nostocales in new habitats have the potential to signif...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00086

    authors: Sukenik A,Hadas O,Kaplan A,Quesada A

    更新日期:2012-03-09 00:00:00

  • SesI May Be Associated with the Invasiveness of Staphylococcus epidermidis.

    abstract::Staphylococcus epidermidis is a commensal bacterium which widely colonizes in human skin and mucous membrane and rarely causes clinically manifested infections. S. epidermidis surface protein I (SesI) is considered to be the major virulence factor of S. epidermidis infection, but its pathogenesis is not clear. Here, w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02574

    authors: Qi X,Jin Y,Duan J,Hao Z,Wang S,Guo Y,Lv J,Hu L,Wang L,Yu F

    更新日期:2018-01-04 00:00:00

  • Analysis of Difference in Microbial Community and Physicochemical Indices Between Surface and Central Parts of Chinese Special-Flavor Baijiu Daqu.

    abstract::Special-flavor Baijiu is a unique Baijiu in Jiangxi Province, China, whose uniqueness mainly depends on the unique production process of special-flavor Baijiu Daqu. However, the microbial structure and physicochemical indices of different parts of the special-flavor Baijiu Daqu are still unknown. This greatly reduces ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.592421

    authors: Chen Y,Li K,Liu T,Li R,Fu G,Wan Y,Zheng F

    更新日期:2021-01-14 00:00:00

  • The Major Immediate-Early Protein IE2 of Human Cytomegalovirus Is Sufficient to Induce Proteasomal Degradation of CD83 on Mature Dendritic Cells.

    abstract::Human cytomegalovirus (HCMV) is the prototypic beta-herpesvirus and widespread throughout the human population. While infection is asymptomatic in healthy individuals, it can lead to high morbidity and mortality in immunocompromised persons. Importantly, HCMV evolved multiple strategies to interfere with immune cell f...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00119

    authors: Heilingloh CS,Grosche L,Kummer M,Mühl-Zürbes P,Kamm L,Scherer M,Latzko M,Stamminger T,Steinkasserer A

    更新日期:2017-02-01 00:00:00

  • Potential for Prebiotics as Feed Additives to Limit Foodborne Campylobacter Establishment in the Poultry Gastrointestinal Tract.

    abstract::Campylobacter as an inhabitant of the poultry gastrointestinal tract has proven to be difficult to reduce with most feed additives. In-feed antibiotics have been taken out of poultry diets due to the negative reactions of consumers along with concerns regarding the generation of antibiotic resistant bacteria. Conseque...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.00091

    authors: Kim SA,Jang MJ,Kim SY,Yang Y,Pavlidis HO,Ricke SC

    更新日期:2019-01-31 00:00:00

  • xCT, not just an amino-acid transporter: a multi-functional regulator of microbial infection and associated diseases.

    abstract::Expression of xCT, a component of the xc (-) amino-acid transporter, is essential for the uptake of cystine required for intracellular glutathione (GSH) synthesis and maintenance of the intracellular redox balance. Therefore, xCT plays an important role not only in the survival of somatic and immune cells, but also in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00120

    authors: Dai L,Noverr MC,Parsons C,Kaleeba JA,Qin Z

    更新日期:2015-02-19 00:00:00

  • Functional Agents to Biologically Control Deoxynivalenol Contamination in Cereal Grains.

    abstract::Mycotoxins, as microbial secondary metabolites, frequently contaminate cereal grains and pose a serious threat to human and animal health around the globe. Deoxynivalenol (DON), a commonly detected Fusarium mycotoxin, has drawn utmost attention due to high exposure levels and contamination frequency in the food chain....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00395

    authors: Tian Y,Tan Y,Liu N,Liao Y,Sun C,Wang S,Wu A

    更新日期:2016-03-30 00:00:00

  • Single-Molecule Sequencing (PacBio) of the Staphylococcus capitis NRCS-A Clone Reveals the Basis of Multidrug Resistance and Adaptation to the Neonatal Intensive Care Unit Environment.

    abstract::The multi-resistant Staphylococcus capitis clone NRCS-A has recently been described as a major pathogen causing nosocomial, late-onset sepsis (LOS) in preterm neonates worldwide. NRCS-A representatives exhibit an atypical antibiotic resistance profile. Here, the complete closed genome (chromosomal and plasmid sequence...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01991

    authors: Simões PM,Lemriss H,Dumont Y,Lemriss S,Rasigade JP,Assant-Trouillet S,Ibrahimi A,El Kabbaj S,Butin M,Laurent F

    更新日期:2016-12-15 00:00:00

  • Same, same but different: symbiotic bacterial associations in GBR sponges.

    abstract::Symbioses in marine sponges involve diverse consortia of microorganisms that contribute to the health and ecology of their hosts. The microbial communities of 13 taxonomically diverse Great Barrier Reef (GBR) sponge species were assessed by DGGE and 16S rRNA gene sequencing to determine intra and inter species variati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00444

    authors: Webster NS,Luter HM,Soo RM,Botté ES,Simister RL,Abdo D,Whalan S

    更新日期:2013-01-18 00:00:00

  • Degradation of Chrysene by Enriched Bacterial Consortium.

    abstract::Chrysene is a high molecular weight (HMW), polycyclic aromatic hydrocarbon (PAH) known for its recalcitrance and carcinogenic properties and sparsely soluble (0.003 mg/L) in aqueous medium. Due to these refractory properties, bioavailability of chrysene is very low and therefore is persistence in the environment escap...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01333

    authors: Vaidya S,Devpura N,Jain K,Madamwar D

    更新日期:2018-06-26 00:00:00

  • iTRAQ Proteomic Analysis Reveals That Metabolic Pathways Involving Energy Metabolism Are Affected by Tea Tree Oil in Botrytis cinerea.

    abstract::Tea tree oil (TTO) is a volatile essential oil obtained from the leaves of the Australian tree Melaleuca alternifolia by vapor distillation. Previously, we demonstrated that TTO has a strong inhibitory effect on Botrytis cinerea. This study investigates the underlying antifungal mechanisms at the molecular level. A pr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01989

    authors: Xu J,Shao X,Wei Y,Xu F,Wang H

    更新日期:2017-10-12 00:00:00

  • Epstein-Barr virus-driven lymphomagenesis in the context of human immunodeficiency virus type 1 infection.

    abstract::Epstein-Barr virus (EBV) is a ubiquitous human γ-herpes virus which establishes a life-long asymptomatic infection in immunocompetent hosts. In human immunodeficiency virus type 1 (HIV-1) infected patients, the impaired immunosurveillance against EBV may favor the development of EBV-related diseases, ranging from lymp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2013.00311

    authors: Petrara MR,Freguja R,Gianesin K,Zanchetta M,De Rossi A

    更新日期:2013-10-18 00:00:00

  • Cell aggregation promotes pyoverdine-dependent iron uptake and virulence in Pseudomonas aeruginosa.

    abstract::In Pseudomonas aeruginosa the Gac signaling system and the second messenger cyclic diguanylate (c-di-GMP) participate in the control of the switch between planktonic and biofilm lifestyles, by regulating the production of the two exopolysaccharides Pel and Psl. The Gac and c-di-GMP regulatory networks also coordinatel...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00902

    authors: Visaggio D,Pasqua M,Bonchi C,Kaever V,Visca P,Imperi F

    更新日期:2015-08-28 00:00:00

  • Component Identification and Functional Analysis of Outer Membrane Vesicles Released by Avibacterium paragallinarum.

    abstract::Avibacterium paragallinarum, the causative agent of infectious coryza, is known to release outer membrane vesicles (OMVs). In the present study, we investigated the composition, bioactivities, and functional properties of the OMVs of A. paragallinarum. Following extraction and purification, the OMVs were observed to b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.518060

    authors: Mei C,Sun AH,Blackall PJ,Xian H,Li SF,Gong YM,Wang HJ

    更新日期:2020-09-25 00:00:00

  • Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein From Halobacillus andaensis and Functional Analysis of Its Conserved Arginine/Aspartate Residues.

    abstract::Arginine-aspartate-aspartate (RDD) family, representing a category of transmembrane proteins containing one highly conserved arginine and two highly conserved aspartates, has been functionally uncharacterized as yet. Here we present the characterization of a member of this family designated RDD from the moderate halop...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00807

    authors: Shao L,Abdel-Motaal H,Chen J,Chen H,Xu T,Meng L,Zhang Z,Meng F,Jiang J

    更新日期:2018-04-25 00:00:00

  • A Novel MSCRAMM Subfamily in Coagulase Negative Staphylococcal Species.

    abstract::Coagulase negative staphylococci (CoNS) are important opportunistic pathogens. Staphylococcus epidermidis, a coagulase negative staphylococcus, is the third leading cause of nosocomial infections in the US. Surface proteins like Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) are major vi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00540

    authors: Arora S,Uhlemann AC,Lowy FD,Hook M

    更新日期:2016-04-29 00:00:00

  • Increased Numbers of Culturable Inhibitory Bacterial Taxa May Mitigate the Effects of Batrachochytrium dendrobatidis in Australian Wet Tropics Frogs.

    abstract::Symbiotic bacterial communities resident on amphibian skin can benefit their hosts. For example, antibiotic production by community members can control the pathogen Batrachochytrium dendrobatidis (Bd) and it is possible for these community members to be used as probiotics to reduce infection levels. In the early 1990s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01604

    authors: Bell SC,Garland S,Alford RA

    更新日期:2018-07-18 00:00:00

  • Oral Immunization with Recombinant Lactobacillus acidophilus Expressing espA-Tir-M Confers Protection against Enterohemorrhagic Escherichia coli O157:H7 Challenge in Mice.

    abstract::Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) causes hemorrhagic colitis and the formation of characteristic attaching and effacing (A/E) lesions in humans. Given the severe sequelae of EHEC O157:H7 infection, it is critical to develop effective vaccines for human use. However, for achieving this goal many...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00417

    authors: Lin R,Zhang Y,Long B,Li Y,Wu Y,Duan S,Zhu B,Wu X,Fan H

    更新日期:2017-03-15 00:00:00

  • Surface Proteins of Staphylococcus epidermidis.

    abstract::Staphylococcus epidermidis is a ubiquitous commensal of human skin. The widespread use of indwelling medical devices in modern medicine provides an opportunity for it to cause infections. Disease causing isolates can come from many different genetic backgrounds. Multiply antibiotic resistant strains have spread global...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01829

    authors: Foster TJ

    更新日期:2020-07-29 00:00:00

  • Detection of Viral Pathogens With Multiplex Nanopore MinION Sequencing: Be Careful With Cross-Talk.

    abstract::Metagenomic sequencing with the Oxford Nanopore MinION sequencer offers potential for point-of-care testing of infectious diseases in clinical settings. To improve cost-effectiveness, multiplexing of several, barcoded samples upon a single flow cell will be required during sequencing. We generated a unique sequencing ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02225

    authors: Xu Y,Lewandowski K,Lumley S,Pullan S,Vipond R,Carroll M,Foster D,Matthews PC,Peto T,Crook D

    更新日期:2018-09-19 00:00:00

  • Structural and molecular determinants of HIV-1 Gag binding to the plasma membrane.

    abstract::Targeting of the Gag polyprotein to the plasma membrane (PM) for assembly is a critical event in the late phase of immunodeficiency virus type-1 (HIV-1) infection. Gag binding to the PM is mediated by interactions between the myristoylated matrix (MA) domain and PM lipids. Despite the extensive biochemical and in vitr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00232

    authors: Vlach J,Saad JS

    更新日期:2015-03-20 00:00:00

  • Salmonella Interaction with and Passage through the Intestinal Mucosa: Through the Lens of the Organism.

    abstract::Salmonella enterica serotypes are invasive enteric pathogens spread through fecal contamination of food and water sources, and represent a constant public health threat around the world. The symptoms associated with salmonellosis and typhoid disease are largely due to the host response to invading Salmonella, and to t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00088

    authors: Hallstrom K,McCormick BA

    更新日期:2011-04-29 00:00:00

  • A brief history of bacterial growth physiology.

    abstract::Arguably, microbial physiology started when Leeuwenhoek became fascinated by observing a Vorticella beating its cilia, my point being that almost any observation of microbes has a physiological component. With the advent of modern microbiology in the mid-19th century, the field became recognizably distinctive with suc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00289

    authors: Schaechter M

    更新日期:2015-04-21 00:00:00

  • Isolation and Genomic Characterization of an Acinetobacter johnsonii Bacteriophage AJO2 From Bulking Activated Sludge.

    abstract::A novel Podoviridae lytic phage AJO2, specifically infecting Acinetobacter johnsonii, was isolated from bulking activated sludge. The one-step growth experiment showed that the latent period and burst size of AJO2 were estimated to be 30 min and 78.1 phages per infected cell, respectively. The viability test indicated...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00266

    authors: Fan N,Yang M,Jin R,Qi R

    更新日期:2019-02-20 00:00:00

  • Variant Signal Peptides of Vaccine Antigen, FHbp, Impair Processing Affecting Surface Localization and Antibody-Mediated Killing in Most Meningococcal Isolates.

    abstract::Meningococcal lipoprotein, Factor H binding protein (FHbp), is the sole antigen of the Trumenba vaccine (Pfizer) and one of four antigens of the Bexsero vaccine (GSK) targeting Neisseria meningitidis serogroup B isolates. Lipidation of FHbp is assumed to occur for all isolates. We show in the majority of a collection ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02847

    authors: da Silva RAG,Karlyshev AV,Oldfield NJ,Wooldridge KG,Bayliss CD,Ryan A,Griffin R

    更新日期:2019-12-19 00:00:00

  • Complementarity of Selective Culture and qPCR for Colistin Resistance Screening in Fresh and Frozen Pig Cecum Samples.

    abstract::Retrospective studies involving the screening of frozen stored collections of samples are commonplace when a new threat emerges, but it has been demonstrated that the freeze-thaw process can affect bacterial viability. The study of colistin-resistant bacteria in human and animal samples is an example of this issue. In...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.572712

    authors: Miguela-Villoldo P,Moreno MA,Hernández M,Rodríguez-Lázaro D,Gallardo A,Borge C,Quesada A,Domínguez L,Ugarte-Ruiz M

    更新日期:2020-11-09 00:00:00

  • LAM Genes Contribute to Environmental Stress Tolerance but Sensibilize Yeast Cells to Azoles.

    abstract::Lam proteins transport sterols between the membranes of different cellular compartments. In Saccharomyces cerevisiae, the LAM gene family consists of three pairs of paralogs. Because the function of paralogous genes can be redundant, the phenotypes of only a small number of LAM gene deletions have been reported; thus,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00038

    authors: Sokolov SS,Vorobeva MA,Smirnova AI,Smirnova EA,Trushina NI,Galkina KV,Severin FF,Knorre DA

    更新日期:2020-01-28 00:00:00

  • Antibiotic Resistance Genes and Bacterial Communities of Farmed Rainbow Trout Fillets (Oncorhynchus mykiss).

    abstract::The rise of antibiotic resistance is not only a challenge for human and animal health treatments, but is also posing the risk of spreading among bacterial populations in foodstuffs. Farmed fish-related foodstuffs, the food of animal origin most consumed worldwide, are suspected to be a reservoir of antibiotic resistan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.590902

    authors: Helsens N,Calvez S,Prevost H,Bouju-Albert A,Maillet A,Rossero A,Hurtaud-Pessel D,Zagorec M,Magras C

    更新日期:2020-12-03 00:00:00

  • Plant and Microbial Responses to Repeated Cu(OH)2 Nanopesticide Exposures Under Different Fertilization Levels in an Agro-Ecosystem.

    abstract::The environmental fate and potential impacts of nanopesticides on agroecosystems under realistic agricultural conditions are poorly understood. As a result, the benefits and risks of these novel formulations compared to the conventional products are currently unclear. Here, we examined the effects of repeated realisti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01769

    authors: Simonin M,Colman BP,Tang W,Judy JD,Anderson SM,Bergemann CM,Rocca JD,Unrine JM,Cassar N,Bernhardt ES

    更新日期:2018-07-31 00:00:00

  • Molecular Targets for Coevolutionary Interactions Between Pacific Oyster Larvae and Their Sympatric Vibrios.

    abstract::Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02067

    authors: Wegner KM,Piel D,Bruto M,John U,Mao Z,Alunno-Bruscia M,Petton B,Le Roux F

    更新日期:2019-09-06 00:00:00