Abstract:
:Hyaluronic acid (HA) preparations are widely used in clinical practice and recent data suggest that commercially available HA-based compounds promote ulcer re-epithelialization and induce pain relief. However, the pathophysiological basis of these effects remains poorly understood. In the present study, we investigated the biophysical, biomolecular and functional properties of a HA preparation combined with a pool of collagen precursor synthetic aminoacids, namely l-proline, l-leucine, l-lysine and glycine (Aminogam®). Hydrodynamic characterization of Aminogam® by size exclusion chromatography-triple detector array (SEC-TDA) revealed an average molecular weight in the range of 700-1700 kDa. Rheological measurements of the 1700kDa Mw lot showed a pseoudoplastic behaviour with a zero-shear viscosity (η0) equal to 90 ± 9 Pa∙s at 25°C and 55 ± 6 Pa∙s at 37°C. Automated time-lapse videomicroscopy studies in a fibroblast-free system demonstrated that 1% (v/v) Aminogam® significantly reduced the healing time of wounded keratinocyte monolayers. In AKGOS assays, Aminogam® stimulated cellular locomotion (chemokinesis) and directional migration (chemotaxis) of keratinocytes. Analysis of microarray data suggested that keratinocytes had a functional neuroendocrine machinery, and this was confirmed by testing the secretion of six neuroactive molecules by ELISA, namely α-MSH, β-endorphins, melatonin, substance P, cortisol, and neurotensin. Interestingly, Aminogam® regulated the production of several neuropeptides, including β-endorphins. In conclusion, our data shed light on the epithelial-dependent mechanisms that underlie the efficacy of Aminogam®, particularly in reference to wound healing and nociception.
journal_name
Cell Adh Migrjournal_title
Cell adhesion & migrationauthors
La Gatta A,D'Agostino A,Schiraldi C,Colella G,Cirillo Ndoi
10.1080/19336918.2018.1494997subject
Has Abstractpub_date
2019-12-01 00:00:00pages
23-32issue
1eissn
1933-6918issn
1933-6926journal_volume
13pub_type
杂志文章abstract::Eph signaling, which arises following stimulation by ephrins, is known to induce opposite cell behaviors such as promoting and inhibiting cell adhesion as well as promoting cell-cell adhesion and repulsion by altering the organization of the actin cytoskeleton and influencing the adhesion activities of integrins. Howe...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.1080/19336918.2015.1107693
更新日期:2015-01-01 00:00:00
abstract::All T cell functions require establishing contacts with other cells. In the last ten years, the immunological synapse, the contact-site between T cells and their partners, has been the object of numerous investigations and recent advances in imaging technologies have provided significant insights into the mechanism of...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:
更新日期:2007-01-01 00:00:00
abstract::Lipocalin-type prostaglandin D synthase (L-PGDS) is one of the most abundant proteins in the cerebrospinal fluid. Nevertheless, its role in the central nervous system is far from clear. Here, we present evidence that L-PGDS induces glial cell migration and morphological changes in vitro and in vivo. We also identified...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.20251
更新日期:2012-05-01 00:00:00
abstract::Renal cell carcinoma (RCC) is the third most frequent malignancy within urological oncology. However, the mechanisms responsible for RCC metastasis are still needed further illustration. Our present study revealed that a seven-transmembrane receptor G-protein coupled estrogen receptor (GPER) was highly detected in var...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/19336918.2014.990781
更新日期:2018-03-04 00:00:00
abstract::Trophoblast migration and invasion through the decidua and maternal uterine spiral arteries are crucial events in placentation. During this process, invasive trophoblast replace vascular endothelial cells as the uterine arteries are remodeled to form more permissive vessels that facilitate adequate blood flow to the g...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.1080/19336918.2015.1120397
更新日期:2016-03-03 00:00:00
abstract::The stroma in human carcinomas consists of extracellular matrix and various types of non-carcinoma cells, mainly leukocytes, endothelial cells, fibroblasts, myofibroblasts and bone marrow-derived progenitors. The tumor-associated stroma actively supports tumor growth by stimulating neo-angiogenesis, as well as prolife...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.20631
更新日期:2012-05-01 00:00:00
abstract::Growth factors regulate a diverse array of cellular functions including proliferation, survival, movement, and the ability to do this often involves interactions with the extracellular matrix (ECM) and particularly heparan sulfate proteoglycans (HSPGs). HSPGs have been shown to sequester growth factors, and to act as ...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.4.4.12427
更新日期:2010-10-01 00:00:00
abstract::Discovered decades ago, extracellular vesicles (EVs) emerge as dedicated organelles, able to deliver protected, specific cellular cues throughout the organism. While virtually every cell can release EVs, cancer cells co-opted this feature and efficiently unleashed them both in the tumor microenvironment and toward hea...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.1080/19336918.2016.1247145
更新日期:2017-03-04 00:00:00
abstract::Angiogenesis, the growth of new blood vessels from pre-existing vessels, is a process involving endothelial cell proliferation, migration and vascular tube formation. One of the key molecules that regulate this process is the integrin beta3 subunit, a cell adhesion receptor that forms a heterodimer with the integrin a...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.3.1.7252
更新日期:2009-01-01 00:00:00
abstract::A deterministic model of dermal wound invasion, which accounts for the platelet-derived growth factor (PDGF) gradient sensing mechanism in fibroblasts mediated by cell surface receptors and the phosphoinositide 3-kinase (PI3K) signal transduction pathway, was previously described (Biophys J 2006; 90:2297-308). Here, w...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.2.2.6511
更新日期:2008-04-01 00:00:00
abstract::The aim of this study was to compare uniaxial traction forces exerted by different cell types using a novel sensor design and to test the dependence of measured forces on cytoskeletal integrity. The sensor design detects forces generated between 2 contact points by cells spanning a gap. The magnitude of these forces v...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.1080/19336918.2015.1120398
更新日期:2016-05-03 00:00:00
abstract::Familial dysautonomia (FD) is a hereditary neuronal disease characterized by poor development and progressive degeneration of the sensory and autonomic nervous system. Majority of FD (99.5%) results from a single nucleotide point mutation in the IKBKAP gene encoding IKAP, also known as elongation protein 1 (ELP1). The...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.2.4.6630
更新日期:2008-10-01 00:00:00
abstract::The proper establishment and organogenesis of the placenta is crucial for intrauterine fetal growth and development. Endometrial invasion by the extravillous trophoblast cells, as well as formation of the syncytiotrophoblast (STB), are of vital importance for placental function. Trophoblast migration and invasion is o...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.1080/19336918.2015.1098800
更新日期:2016-03-03 00:00:00
abstract::Laminins (LM) are extracellular matrix molecules that contribute to and are required for the formation of basement membranes. They participate in the modulation of epithelial/mesenchymal interactions and are implicated in organogenesis and maintenance of organ homeostasis. Among the LM molecules, the LM α5 chain (LMα5...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.22236
更新日期:2013-01-01 00:00:00
abstract::During brain development, billions of neurons organize into highly specific circuits. To form specific circuits, neurons must build the appropriate types of synapses with appropriate types of synaptic partners while avoiding incorrect partners in a dense cellular environment. Defining the cellular and molecular rules ...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.1080/19336918.2014.1000072
更新日期:2015-01-01 00:00:00
abstract::Adherens junctions (AJs) are essential for the maintenance of epithelial homeostasis and a key factor in the regulation of cell migration and tumor progression. AJs maintain cell-cell adhesion by linking transmembrane proteins to the actin cytoskeleton. Additionally, they participate in recruitment of signaling recept...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.21326
更新日期:2012-07-01 00:00:00
abstract::Cell migration requires a highly coordinated interplay between specialized plasma membrane adhesion complexes and the cytoskeleton. Protein phosphorylation/dephosphorylation modifications regulate many aspects of the integrin-cytoskeleton interdependence, including their coupling, dynamics, and organization to support...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.26375
更新日期:2013-09-01 00:00:00
abstract::Cellular motility is a fundamental process essential for embryonic development, wound healing, immune responses, and tissues development. Cells are mostly moving by crawling on external, or inside, substrates which can differ in their surface composition, geometry, and dimensionality. Cells can adopt different migrati...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.1080/19336918.2016.1170260
更新日期:2016-09-02 00:00:00
abstract::Eph receptors and their membrane-bound ligands are intimately involved in the control of morphogenic processes during embryonic development and adult tissue homeostasis. By their ability to orchestrate cell migration, pattern formation and tissue integrity they are also prone to be involved in carcinogenic growth. In ...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.20154
更新日期:2012-03-01 00:00:00
abstract::The leukocyte adhesion cascade is of critical importance for both the maintenance of immune homeostasis and the ability of immune cells to perform effector functions. Here, we present data showing CD4+ T cells migrate upstream (against the direction of flow) after completing the leukocyte adhesion cascade on surfaces ...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.1080/19336918.2019.1587269
更新日期:2019-12-01 00:00:00
abstract::The critical role of migration and invasion in cancer metastasis warrants new therapeutic approaches targeting the machinery regulating cell migration and invasion. While 2-dimensional (2D) models have helped identify a range of adhesion molecules, cytoskeletal components and regulators that are potentially important ...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.21559
更新日期:2012-09-01 00:00:00
abstract::It has long been thought that the same molecules guide both trunk neural crest cells and motor axons as these cell types grow and extend to their target regions in developing embryos. There are common territories that are navigated by these cell types: both cells grow through the rostral portion of the somitic sclerot...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.4.4.13594
更新日期:2010-10-01 00:00:00
abstract::Visceral adipose tissue (VAT) may play a critical role in atherosclerotic cardiovascular disease. The goal of this study was to determine the effect of human VAT-released pro‑inflammatory cytokines on monocyte adhesion to the endothelium. The cytokine effects on monocyte adhesion to the endothelial cells (ECs) were te...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.1080/19336918.2019.1644856
更新日期:2019-12-01 00:00:00
abstract::Changes in cell shape are associated with a variety of processes including cell migration, axon outgrowth, cell division and vesicle trafficking. C. elegans UNC-53 and its vertebrate homologs, the Navigators, are required for the migration of cells and the outgrowth of neuronal processes. The identification of novel m...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.3.4.9451
更新日期:2009-10-01 00:00:00
abstract::Activation of platelet derived growth factor (PDGF) receptors causes context-dependent cellular responses, including proliferation and migration, and studies in model organisms have demonstrated that this receptor family (PDGFRα and PDGFRβ) is required in many mesenchymal and migratory cell populations during embryoni...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.4.4.12829
更新日期:2010-10-01 00:00:00
abstract::In the cortex of a motile cell, membrane-anchored actin filaments assemble into structures of varying shape and function. Filopodia are distinguished by a core of bundled actin filaments within finger-like extensions of the membrane. In a recent paper by Medalia et al(1) cryo-electron tomography has been used to recon...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.1.3.4662
更新日期:2007-07-01 00:00:00
abstract::Cancer exosomes are gaining considerable amount of attention in basic and applied clinical research for their established role in the modulation of the tumor niche, and their broad-range contribution to tumor-host cross-talk. Supporting evidence to their role in tumorigenesis comes from the observation that exosome se...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.1080/19336918.2016.1225632
更新日期:2017-03-04 00:00:00
abstract::The class-specific transcription factors Knot and Cut act during dendrite arbor development to define the characteristic dendrite branching pattern of the Drosophila class IV dendritic arborisation sensory neurons. Knot mediates dendrite arbor outgrowth and branching via a microtubule-based program that includes upreg...
journal_title:Cell adhesion & migration
pub_type: 杂志文章
doi:10.4161/cam.2.2.6395
更新日期:2008-04-01 00:00:00
abstract::The hypotheses emerging from basic research on colorectal liver metastases must be tested in clinical situations for the adaptation of current treatment strategies. Pre-metastatic niches have been shown to exist in human colorectal synchronous metastases, with the liver parenchyma adjacent to the synchronous liver met...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.23247
更新日期:2012-11-01 00:00:00
abstract::Cellular studies have long been performed on the bench top, within Petri dishes and flasks that expose cells to surroundings that differ greatly from their native environment. The complexity of a human tissue is such that to truly replicate a cell's physiologic microenvironment in vitro is currently impossible. It is ...
journal_title:Cell adhesion & migration
pub_type: 杂志文章,评审
doi:10.4161/cam.21076
更新日期:2012-05-01 00:00:00