Abstract：
:Circular RNAs characterize a class of widespread and diverse endogenous RNAs which are non-coding RNAs that are made by back-splicing events and have covalently closed loops with no polyadenylated tails. Various indications specify that circular RNAs (circRNAs) are plentiful in the human transcriptome. However, their participation in biological processes remains mostly undescribed. To date thousands of circRNAs have been revealed in organisms ranging from Drosophila melanogaster to Homo sapiens. Functional studies specify that these transcripts control expression of protein-coding linear transcripts and thus encompass a key component of gene expression regulation. This chapter provide a comprehensive overview on functional validation of circRNAs. Furthermore, we discuss the recent modern methodologies for the functional validation of circRNAs such as RNA interference (RNAi) gene silencing assay, luciferase reporter assays, circRNA gain-of-function investigation via overexpression of circular transcript assay, RT-q-PCR quantification, and other latest applicable assays. The methods described in this chapter are demonstrated on the cellular model.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Shanmugapriya,Huda HA,Vijayarathna S,Oon CE,Chen Y,Kanwar JR,Ng ML,Sasidharan Sdoi
10.1007/978-981-13-1426-1_8subject
Has Abstractpub_date
2018-01-01 00:00:00pages
95-105eissn
0065-2598issn
2214-8019journal_volume
1087pub_type
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