Abstract:
:[3H]5-HT exhibited specific binding in membrane preparations of Hymenolepis diminuta. The specific binding was saturable, reversible and temperature dependent. A non-linear Scatchard plot was obtained in a concentration range of 11 nM - 1000 nM [3H]5-HT, which could be resolved into sites having apparent dissociation constants (KD) of 0.10 microM and 6.25 microM for the high-affinity and low-affinity components, respectively. The latter could be selectively eliminated by binding [3H]5-HT to H. diminuta membranes in the presence of 10(-3) M nitroimipramine. Drug displacement studies, using 0.20 microM and 2.0 microM [3H]5-HT, revealed that while low-affinity [3H]5-HT binding was displaced by unlabelled 5-HT and inhibitors of 5-HT uptake, high affinity [3H]5-HT binding was affected only by tryptamine derivatives and, to a lesser extent, methysergide. In addition, high-affinity binding was stimulated by MgCl2 while low-affinity binding showed sodium-dependency. The data implicate the low-affinity site as a putative 5-HT transporter and the high-affinity site as a putative 5-HT 1 receptor. Exposure of H. diminuta membranes to 5-HT resulted in a 3-4 fold stimulation of cAMP levels. The EC 50 for the 5-HT-induced activation of adenylate cyclase (0.76 microM) was of the same order of magnitude as the apparent KD for high-affinity binding. Furthermore, the order of drug potency for the elevation of cAMP levels by 5-HT agonists and reversal by 5-HT antagonists was identical to the order of drug potency for the inhibition of high-affinity binding, suggesting linkage of the putative 5-HT 1 receptor to adenylate cyclase in H. diminuta.
journal_name
Life Scijournal_title
Life sciencesauthors
Ribeiro P,Webb RAdoi
10.1016/0024-3205(87)90303-1subject
Has Abstractpub_date
1987-02-23 00:00:00pages
755-68issue
8eissn
0024-3205issn
1879-0631pii
0024-3205(87)90303-1journal_volume
40pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:We investigated the involvement of the renin angiotensin system (RAS) on the cardiorespiratory control in rats from dams fed with a low-protein diet. MAIN METHODS:Male offspring were obtained from dams fed a normoprotein diet (NP, 17% casein) and low-protein diet (LP, 8% casein) during pregnancy and lactation. Di...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118574
更新日期:2020-12-15 00:00:00
abstract:AIMS:This study investigates the effect of a new combination of glucosamine hydrochloride, chondroitin sulfate, methylsulfonylmethane, Harpagophytum procumbens root extract (standardized to 3% harpagoside) and bromelain extract (GCMHB) on formalin-induced damage to cartilage tissue in the rat knee joint and evaluates t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.03.012
更新日期:2015-06-01 00:00:00
abstract:AIMS:microRNA-96 (miR-96) is considered as a tumor suppressor in multiple malignancies. Some studies have indicated that EZRIN could be regulated by the miR-96 in several kinds of cancer cells. However, the function of miR-96 in human osteosarcoma has not been investigated intensively so far. In this study, we mainly e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.04.012
更新日期:2018-06-15 00:00:00
abstract::The object of this study was to develop an assay for platelet activating factor (PAF) in rat plasma, and to utilise this to determine the effects of dietary fish oil on PAF in normotensive and spontaneously hypertensive rats. Measurement of platelet activating factor in blood plasma has proved difficult because of its...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90143-8
更新日期:1986-05-19 00:00:00
abstract::Tissue-type plasminogen activator (tPA) is a key enzyme in the fibrinolysis system and the regulation of its expression has been extensively studied in cultured vascular endothelial cells. Many kinds of supplements including growth factors are needed, however, to keep endothelial cells viable, which leads the culture ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01513-2
更新日期:2002-02-08 00:00:00
abstract:AIMS:The aim of the present study was to evaluate the oral resveratrol effects associated with diet and physical training changes on anthropometric and biochemical parameters. MAIN METHODS:25 individuals aged from 30 to 60 years old; with Body Mass Index (BMI) ≥ 30 kg/m2 were included in the study. Following the prima...
journal_title:Life sciences
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.lfs.2020.117962
更新日期:2020-09-01 00:00:00
abstract::This paper gives a short account of the mechanisms regulating choline production. Although choline is absorbed from the GI tract and biosynthesized in the liver, its subsequent metabolism to choline esters and phospholipids seems to dominate in importance as to its regulatory role in maintaining a constant source of f...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(96)00181-6
更新日期:1996-01-01 00:00:00
abstract::Cardiovascular effects of the essential oil of Croton zehntneri (EOCZ) were investigated in conscious rats. In these preparations, intravenous (i.v.) injections of EOCZ (1-20 mg kg(-1)) and its main constituents anethole and estragole (both at 1-10 mg kg(-1)) elicited brief and dose-dependent hypotension and bradycard...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.09.042
更新日期:2006-04-11 00:00:00
abstract::In the present study, we elucidated the possible role of hemodynamic parameters and chemical factors in the development of ventricular hypertrophy (VH) following chronic nitric oxide (NO) deprivation with Nomega-nitro-L-arginine methyl ester (L-NAME). Impedance spectral analysis was used to obtain the arterial hemodyn...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.04.061
更新日期:2005-11-26 00:00:00
abstract:AIMS:Parathyroid hormone-related protein (PTHrP) is a peptide growth factor produced in a wide range of tissues from brain and parathyroid, to kidney and uterus. The purpose of this study was to determine whether the adrenal cortical hormones, hydrocortisone (cortisol), modulate PTHrP expression and glucocorticoid rece...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.08.001
更新日期:2011-10-24 00:00:00
abstract:AIM:Exosomes, the nano-units (<200 nm), released from diverse cell types in the extracellular body fluid, possess non-immunogenic property and ability to cross the blood-brain barrier (BBB). Since exosomes carry biological information from their cells of origin, we hypothesize that priming cells with potential therapeu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.04.018
更新日期:2014-06-27 00:00:00
abstract:AIM:Although constitutive autophagy is linked to redox state and participates in cell homeostasis, it is scarcely known if redox state, autophagy, and lysosomal function depend on sex, a factor that largely influences health and diseases. Therefore, we evaluated the existence of sex differences in redox state and const...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.06.001
更新日期:2013-08-06 00:00:00
abstract::Years of researches have demonstrated that the imbalance of Th17 and Tregs contribute to the thyroid autoimmunity and the severity of autoimmune thyroid disease (AITD). The underlying mechanism comprises inherent genetic predisposition, abnormality of Th17 and Treg related biological molecules, and gut microbiota diso...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2017.11.026
更新日期:2018-01-01 00:00:00
abstract::U-54494A, a 1,2-diamine anticonvulsant, and U-50488H, a structurally related agonist for opiate kappa receptors, were tested for effects on spontaneous and glutamate-evoked firing rates in cerebral cortex of urethane-anesthetized male Sprague-Dawley rats. Iontophoretic application of 1,2-diamines, glutamate diethyl et...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90322-g
更新日期:1992-01-01 00:00:00
abstract::The ATP-binding cassette (ABC) and solute carrier (SLC) transporter families consist of common drug transporters that mediate the efflux and uptake of drugs, respectively, and play an important role in the absorption, distribution, metabolism and excretion of drugs in vivo. Rheumatoid arthritis (RA) is an autoimmune d...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118907
更新日期:2021-01-08 00:00:00
abstract:AIMS:In this study, we conducted a meta-analysis to systematically compare the diagnostic accuracy of IGRAs performed for extrasanguinous body fluids with that performed for blood in the diagnosis of TB. MAIN METHODS:Multiple English and Chinese databases were searched up to November 2017. Studies that complied with t...
journal_title:Life sciences
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.lfs.2018.02.004
更新日期:2018-03-15 00:00:00
abstract::Obesity leads to various changes in the body. Among them, the existing inflammatory process may lead to an increase in the production of reactive oxygen species (ROS) and cause oxidative stress. Oxidative stress, in turn, can trigger mitochondrial changes, which is called mitochondrial dysfunction. Moreover, excess nu...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2017.11.019
更新日期:2018-01-01 00:00:00
abstract:AIMS:The purposes of this study were to determine whether Cervi Pantotrichum Cornu (CPC) has osteogenic activities in human osteoblastic MG-63 cells and to investigate the underlying molecular mechanism. MAIN METHODS:The effects of CPC on alkaline phosphatase activity, collagen synthesis, and calcium deposits were mea...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.11.002
更新日期:2014-01-14 00:00:00
abstract:AIMS:Despite the advanced cancer treatments, there is increased resistance to chemotherapy and subsequent mortality. In lack of reliable data in monolayer cultures and animal models, researchers are shifting to 3D cancer spheroids, which represents the in vivo robust tumour morphology. Calcium is essential in cell sign...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118291
更新日期:2020-11-01 00:00:00
abstract:AIMS:Head and neck squamous cell carcinoma (HNSCC) is an highly aggressive tumor with heterogeneous prognosis. We here report that immune-related genes (IRGs) could effectively distinguish prognostically different HNSCC patients. MATERIALS AND METHODS:MRNA levels of 1333 IRGs that from ImmPort database in HNSCC sample...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117906
更新日期:2020-09-01 00:00:00
abstract::The nephrotic syndrome is a renal disease characterized by proteinuria, hypoproteinemia, edema and hyperlipidemia. It has been reported that female nephrotic rats are characterized by loss of the oestrus cycle, follicle atresia, low gonadotropin and steroid concentrations; particularly, undetectable estradiol levels. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.02.023
更新日期:2006-07-10 00:00:00
abstract::Increase of intracellular reactive oxygen species (ROS) has been proposed to cause endothelial injury, and oxidized LDL (oxLDL) actions are associated with an early increase of ROS. Estrogen protects vascular cells partly via its antioxidant effects and by preventing lipid peroxidation. However, whether it can inhibit...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01486-2
更新日期:2001-12-14 00:00:00
abstract::Losartan, a recently developed nonpeptide angiotensin II (Ang II) receptor antagonist, was administered orally to 10-week-old spontaneously hypertensive rats (SHR) for 2 weeks. Cardiac weight and tissue Ang II, as well as plasma renin activity (PRA) and Ang II, were determined. Treatment with Losartan (10 mg/kg per da...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90589-h
更新日期:1992-01-01 00:00:00
abstract::The effects of aging on contractile response to angiotensin II and tachyphylaxis to it were investigated using aortic strips from rats aged 1.5, 4 and 22 months. Whether the endothelium was present or not, the contractile response to angiotensin II was greater and tachyphylaxis to it was less in 1.5-month-old rats tha...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90549-7
更新日期:1990-01-01 00:00:00
abstract::A phencyclidine (PCP) receptor binding site has been solubilized in an active ligand-binding state from rat cerebral cortical membranes with sodium deoxycholate. Optimal receptor solubilization occurs at a detergent/protein ratio of 0.5 (w/w); for 5 mg protein/ml solubilized with 0.25% sodium deoxycholate, about 60% o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90567-5
更新日期:1989-01-01 00:00:00
abstract:AIM:A variety of evidence suggests that vitamin D can prevent the development of multiple sclerosis (MS). TGF-β pathway genes also play important roles in MS. Here, we aim to study whether vitamin D affects TGF-β pathway gene expression and Expanded Disability Status Scale (EDSS) scores in MS patients. MAIN METHODS:A ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.05.017
更新日期:2015-08-01 00:00:00
abstract::The effect of prior incubation with a single concentration of isoproterenol (10(-4) M) for 2 hours at 37 degrees C on isoproterenol-stimulated cyclic AMP accumulation in intact lymphocytes from young, old and subjects with Alzheimer's disease was studied. In lymphocytes from all three subjects groups prior incubation ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90277-7
更新日期:1984-10-22 00:00:00
abstract::Although both the incidence and the mortality rate of breast cancer is rising, there is no potent and practical option for the treatment of these patients, particularly in advanced stages. One of the most critical challenges for treatment is the presence of complicated and extensive tumor escape mechanisms in the tumo...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.117437
更新日期:2020-04-15 00:00:00
abstract::1,25-dihydroxyvitamin D3 has important physiological effects such as calcium transport and cell growth and differentiation. These biological effects are mediated by their binding to specific intracellular receptor termed vitamin D receptor (VDR). VDR mRNA expression has been demonstrated in several tissues, but to dat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00586-3
更新日期:1997-01-01 00:00:00
abstract::Phosphatidylinositol (PI) specific phospholipase C treatment of rabbit platelets caused 95% release of acetylcholinesterase in the supernatant and 4 to 6% hydrolysis of membrane PI in 2 min. Under these conditions there was no cell lysis as monitored by lack of lactate dehydrogenase activity in the medium. The phospho...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90590-4
更新日期:1986-02-24 00:00:00