[In vitro sensitivity testing of human kidney tumors to cytostatic drugs with a rapid in vitro test].

Abstract:

:We present a new modified in vitro culture assay for primary human renal cell carcinoma similar to the 'soft agar clonogenic assay'. However, the carrying out and expense of metrology are more simplified, allowing tumor-specific chemotherapeutic drug sensitivity testing under easier conditions. Twelve different samples of human renal carcinoma and one sample of a transitional cell carcinoma of the renal pelvis were tested for in vitro chemotherapy sensitivity using this modified colony-forming assay. The rate of tumor cells establishing in culture was 100%. Corresponding to clinical experience we observed a nearly complete resistance to the cytotoxic effects of standard chemotherapeutic agents at usual plasma concentrations in man. Only higher concentrated chemotherapeutic drugs showed in vitro therapeutic effects. The assay described here lasts about 7 days, which is beneficial from the clinical point of view. The modification of this in vitro chemotherapeutic drug treatment is unlimited for plasma concentration and duration of standard and experimental chemotherapeutic agents, drug combination and so on. So we have interesting scientific steps which could not have been undertaken under usual clinical-empirical conditions.

journal_name

Urol Int

journal_title

Urologia internationalis

authors

de Riese W,Aeikens B,Lenis G,Schindler E

doi

10.1159/000281178

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

112-6

issue

2

eissn

0042-1138

issn

1423-0399

journal_volume

41

pub_type

杂志文章
  • Pubovaginal cutaneous fascial sling procedure for stress urinary incontinence: 10 years' experience.

    abstract:OBJECTIVES:There is a considerable lack of consensus regarding indications and long-term efficacy of the many techniques for treating urinary stress incontinence. We report the long-term results of a modified pubovaginal sling procedure. METHODS:From 1989 to 1998, 129 consecutive patients underwent a pubovaginal sling...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000077685

    authors: Reichelt O,Weirich T,Wunderlich H,Schubert J

    更新日期:2004-01-01 00:00:00

  • Does ischemia-induced prostate damage during cardiac surgery involving cardiopulmonary bypass cause bladder outlet obstruction?

    abstract:OBJECTIVE:This study sought to investigate whether ischemia-induced prostate damage during cardiac surgery involving cardiopulmonary bypass causes bladder outlet obstruction. MATERIALS AND METHODS:The study involved 37 men who underwent elective cardiac surgery involving cardiopulmonary bypass. Prostate-specific antig...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000084434

    authors: Guvel S,Turkoz R,Egilmez T,Kilinc F,Yaycioglu O,Atalay H,Ozkardes H

    更新日期:2005-01-01 00:00:00

  • Clinical predictors of stone fragmentation using slow-rate shock wave lithotripsy.

    abstract:AIM:To determine efficacy and clinical predictors of stone fragmentation when using slow-rate shock wave lithotripsy (SWL). PATIENTS AND METHODS:In total, 116 patients with radiopaque urinary tract stones were randomized into two groups: 57 patients were treated with SWL at 90 shocks/min, and 59 patients were treated ...

    journal_title:Urologia internationalis

    pub_type: 杂志文章,随机对照试验

    doi:10.1159/000106324

    authors: Li WM,Wu WJ,Chou YH,Liu CC,Wang CJ,Huang CH,Lee YC

    更新日期:2007-01-01 00:00:00

  • Virtual bladder tumor transurethral resection: an objective evaluation tool to overcome learning curves with and without photodynamic diagnostics.

    abstract:INTRODUCTION:Virtual reality (VR) simulation has been known to shorten learning curves. The aim of this research was to determine whether VR transurethral bladder tumor resection (TURBT) with and without photodynamic diagnostic (PDD) can improve endourological skills in novice and trained endoscopists. MATERIALS AND M...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000328218

    authors: Kruck S,Bedke J,Hennenlotter J,Amend B,Merseburger A,Stenzl A,Sievert KD

    更新日期:2011-01-01 00:00:00

  • Application of dorsal preputial island flap in meatoplasty.

    abstract:INTRODUCTION AND OBJECTIVES:Meatal stenosis usually presents in adults with a history of urethral trauma or inflammation or as a complication after hypospadias repair. The objective of this study is to present the correction of meatal stenosis using a preputial island flap, created on the dorsal surface of the penis. ...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000102905

    authors: Radopoulos D,Dimitriadis G,Tahmatzopoulos A,Vasilakakis I,Malioris A

    更新日期:2007-01-01 00:00:00

  • Bilateral percutaneous nephrolithotomy in a patient with hemophilia A disorder.

    abstract::A 50-year-old man with hemophilia A presented with recurrent hematuria due to renal stone disease. He was receiving approximately 50,000 units of recombinant factor (rF) VIII concentrate every year due to hematuria. Between 1996 and 2002, his serum creatinine level increased from 0.7 to 1.2 ng/ml. In an effort to reso...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000100846

    authors: Kural AR,Demirkesen O,Alici B,Obek C,Tunc B,Ozkan B

    更新日期:2007-01-01 00:00:00

  • Accuracy of magnetic resonance imaging compared to computerized tomography and renal selective angiography in preoperatively staging renal cell carcinoma.

    abstract::During 1986 and 1987, 47 patients with renal cell carcinoma were evaluated preoperatively with CT, angiography and MRI. The preoperative tumor stage (T), lymph node metastases and venous involvement determined with the three methods were compared to the operative and histopathological findings. For T stage, angiograph...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000282219

    authors: Constantinides C,Recker F,Bruehlmann W,von Schulthess G,Goebel N,Zollikofer C,Jaeger P,Hauri D

    更新日期:1991-01-01 00:00:00

  • Health-related quality of life in patients with hormone refractory prostate cancer receiving gefitinib.

    abstract:OBJECTIVES:Improvements in quality of life (QoL) and disease-related symptoms are key goals in the treatment of hormone refractory prostate cancer (HRPC). Our aim was to evaluate the impact of gefitinib on QoL of patients with HRPC. METHODS:Patients with HRPC received gefitinib 250 mg daily in addition to antiandrogen...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000200800

    authors: Curigliano G,Spitaleri G,De Cobelli O,Scardino E,Sbanotto A,de Braud F

    更新日期:2009-01-01 00:00:00

  • Childhood urolithiasis: urological management of upper tract calculi in the era of extracorporeal shock-wave lithotripsy.

    abstract:OBJECTIVE:To evaluate the management of urolithiasis in children since the development of extracorporeal shock-wave lithotripsy (ESWL). METHODS:Between 1988 and 1994, 37 children, aged from 2 to 15 years (mean 10), with upper tract urolithiasis were evaluated and treated. Lithogenic metabolic disorders or anomalies of...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000282882

    authors: Robert M,Drianno N,Guiter J,Averous M,Grasset D

    更新日期:1996-01-01 00:00:00

  • Clinicopathological study on end-stage reflux nephropathy in renal-transplanted children.

    abstract::Five children with end-stage reflux nephropathy underwent kidney transplantation at our clinic. Reflux nephropathy was studied clinically and histologically. All children had proteinuria before starting hemodialysis, and hypertension was present in 2 cases. Three children underwent antireflux operations prior to trans...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000281672

    authors: Kunikata S,Ishii T,Nishioka T,Uemura T,Wakabayashi A,Kanda H,Matsuura T,Kohri K,Akiyama T,Kurita T

    更新日期:1990-01-01 00:00:00

  • Does Determining Tumor Markers from the Testicular Vein Enable Better Diagnosis and Prognosis?

    abstract:INTRODUCTION:β-HCG has been the only tumor marker evaluated in testicular vein (VT) blood until now. OBJECTIVE:To evaluate the correlation between the tumor markers β-HCG, AFP, PLAP, and LDH from the VT and peripheral blood as well as their significance in predicting tumor recurrence and tumor stage. METHODS:Patients...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000511649

    authors: Zauner T,Heidler S,Lusuardi L,Albrecht W,Wimpissinger F

    更新日期:2020-12-17 00:00:00

  • Postoperative Quality of Life in Patients with Ileal Neobladder at Short-, Intermediate- and Long-Term Follow-Up.

    abstract:BACKGROUND:To evaluate the difference at different steps of follow-up of the postoperative quality of life (QoL) in patients who had undergone radical cystectomy and ileal orthotopic neobladder derivation. PATIENTS AND METHODS:A multicentric, cross-qualitative study was performed in 5 Italian centers of reference for ...

    journal_title:Urologia internationalis

    pub_type: 杂志文章,多中心研究

    doi:10.1159/000443185

    authors: D''Agostino D,Racioppi M,Pugliese D,Ragonese M,Di Gianfrancesco L,Filianoti A,Palermo G,Belgrano E,Siracusano S,Niero M,Imbimbo C,Iafrate M,Artibani W,Cerruto MA,Talamini R,Bassi P

    更新日期:2016-01-01 00:00:00

  • Laparoscopic pelvic lymphadenectomy in prostatic cancer: an analysis of seventy consecutive cases.

    abstract::From July 1992 to April 1994, 70 patients with prostatic cancer underwent laparoscopic pelvic lymph-node dissection (LPLD) before retropubic radical prostatectomy or afterloading therapy in our clinic. After an initial learning phase, the accuracy of LPLD was excellent, with only 3.0% residual nodes and no false-negat...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000282756

    authors: Klän R,Meier T,Knispel HH,Wegner HE,Miller K

    更新日期:1995-01-01 00:00:00

  • Managing mesh erosion after abdominal pelvic organ prolapse repair: ten years' experience in a single center.

    abstract:OBJECTIVE:To report conservative and surgical strategies for treatment of mesh erosion after pelvic organ prolapse (POP) repair. METHODS:Between 1998 and 2008, 179 patients underwent integral pelvic floor reconstruction for advanced POP in our tertiary urogynecological unit. Patients' charts and follow-up data were en...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000324243

    authors: Costantini E,Zucchi A,Lazzeri M,Del Zingaro M,Vianello A,Porena M

    更新日期:2011-01-01 00:00:00

  • Transrectal ultrasound versus magnetic resonance imaging in the estimation of prostate volume as compared with radical prostatectomy specimens.

    abstract:INTRODUCTION:There are relatively few studies that compare the use of transrectal ultrasound (TRUS) to magnetic resonance imaging (MRI) to estimate the prostate volume. In this study, we compared the prostate volumes measured with MRI and TRUS with a surgical specimen volume. PATIENTS AND METHODS:Seventy-three patient...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000100836

    authors: Lee JS,Chung BH

    更新日期:2007-01-01 00:00:00

  • Value of serum laminin P1 as a diagnostic and monitoring parameter in transitional cell carcinoma of the bladder.

    abstract::In a combined (cross-sectional and longitudinal) study, the serum concentration of laminin P1 was measured by radioimmunoassay in 16 patients with benign inflammatory bladder disorders and 47 patients with transitional cell carcinoma of the bladder. The results were compared with the serum laminin P1 values in 50 heal...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000282410

    authors: AbouFarha KM,Menheere PP,Nieman FH,Arends JW,Janknegt RA

    更新日期:1992-01-01 00:00:00

  • Perioperative complications and 90-day mortality of radical cystectomy in the elderly (75+): a retrospective, multicentre study.

    abstract:OBJECTIVE:To assess perioperative complications and 90-day mortality of radical cystectomy (RC) in elderly patients with muscle-invasive bladder cancer (MIBC). MATERIALS AND METHODS:This is a retrospective, multicentre (n = 11) study of a consecutive series of patients ≥75 years who underwent RC for MIBC between 2006 ...

    journal_title:Urologia internationalis

    pub_type: 杂志文章,多中心研究

    doi:10.1159/000357127

    authors: Berger I,Martini T,Wehrberger C,Comploj E,Ponholzer A,Wolfgang M,Breinl E,Dunzinger M,Hofbauer J,Höltl W,Jeschke K,Krause S,Kugler W,Pauer W,Rauchenwald M,Pycha A,Madersbacher S

    更新日期:2014-01-01 00:00:00

  • Current information on sacral neuromodulation and botulinum toxin treatment for refractory idiopathic overactive bladder syndrome: a review.

    abstract::The overactive bladder syndrome (OAB) negatively affects the daily life of many people. Conservative treatments, such as antimuscarinics, do not always lead to sufficient improvement of the complaints and/or are often associated with considerable side effects resulting in treatment failure. In the case of failure or i...

    journal_title:Urologia internationalis

    pub_type: 杂志文章,评审

    doi:10.1159/000288223

    authors: Leong RK,De Wachter SG,van Kerrebroeck PE

    更新日期:2010-01-01 00:00:00

  • Evaluation of seminal vesicle characteristics by ultrasonography before and after ejaculation.

    abstract::Twenty males underwent transrectal ultrasonography before and after ejaculation to examine possible alterations that could influence interpretation of seminal vesicle ultrasonography. The preejaculation length of 35 mm was significantly (p < 0.05) decreased to 30 mm after ejaculation. The mean width of the seminal ves...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000282404

    authors: Fuse H,Okumura A,Satomi S,Kazama T,Katayama T

    更新日期:1992-01-01 00:00:00

  • Klinefelter's syndrome associated with unilateral cryptorchidism and chordee without hypospadia.

    abstract::We report a case of Klinefelter's syndrome associated with unilateral cryptorchidism and chordee without hypospadia. The literature is reviewed, and hormonal conditions of Klinefelter's syndrome during childhood are discussed. ...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000282369

    authors: Sasagawa I,Nakada T,Hashimoto T,Ishigooka M,Adachi Y,Kubota Y,Watanabe H

    更新日期:1992-01-01 00:00:00

  • Diagnosing the location of carcinoma in situ (CIS) of the urinary bladder using pirarubicin hydrochloride.

    abstract:INTRODUCTION:No established technique for locating solitary carcinoma in situ (CIS) of the urinary bladder or CIS accompanying bladder cancer has been determined. Here we investigated whether the location of CIS of the urinary bladder can be macroscopically ascertained by instilling pirarubicin hydrochloride (THP) into...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000083555

    authors: Uchikoba T,Horiuchi K,Oka F,Sato M,Tsuboi N,Ohaki Y,Nishimura T

    更新日期:2005-01-01 00:00:00

  • Comparison of lumbar flank approach and transperitoneal approach for radical nephrectomy.

    abstract::A retrospective analysis based on Robson's tumor stage classification was performed on 56 patients with renal cell carcinoma who had undergone radical nephrectomy through a lumbar flank approach and 35 who had through a transperitoneal approach. The 5-year survival rates of patients with nephrectomy through the lumbar...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000281772

    authors: Sugao H,Matsuda M,Nakano E,Seguchi T,Sonoda T

    更新日期:1991-01-01 00:00:00

  • Ipsilaterality of motor innervation of canine urethral sphincter.

    abstract::The functional activity of the sphincter muscle of the urethra is known to be controlled largely by the hypogastric and pudendal nerves. It remains unknown, however, whether innervation of the muscle by these peripheral nerves is ipsi- or bilateral. In an attempt to answer this question urethral closure pressure was d...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000281328

    authors: Morita T,Kizu N,Kondo S,Dohkita S,Tsuchida S

    更新日期:1988-01-01 00:00:00

  • Verapamil attenuates renal tubular apoptosis in response to partial unilateral ureteral obstruction.

    abstract:INTRODUCTION:Partial unilateral ureteral obstruction (PUUO) is the type of obstruction that is most often encountered in pediatric clinical practice. The majority of our knowledge on PUUO has been derived from experimental studies and the effects of PUUO on the kidney have still been a source of continual investigation...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000111736

    authors: Topcu SO,Erbagci A,Erturhan S,Yagci F,Ucak R

    更新日期:2008-01-01 00:00:00

  • Development of Predictive Risk Models of Postpartum Stress Urinary Incontinence for Primiparous and Multiparous Women.

    abstract:OBJECTIVES:To develop risk predictive models of postpartum stress urinary incontinence (SUI) for both primiparous and multiparous women. MATERIALS AND METHODS:From July 2016 to July 2017, 815 singleton pregnant women without incontinence before pregnancy who were 18 years or older and admitted to 2 hospitals in Shenzh...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000508416

    authors: Chen L,Luo D,Chen X,Jin M,Yu X,Cai W

    更新日期:2020-01-01 00:00:00

  • da Vinci and Open Radical Prostatectomy: Comparison of Clinical Outcomes and Analysis of Insurance Costs.

    abstract:PURPOSE:To assess clinical outcomes and reimbursement costs of open and robotic-assisted radical prostatectomies in Germany. METHODS:Perioperative data of 499 open (2003-2006) and 932 (2008-2010) robotic-assisted radical prostatectomies as well as longitudinal reimbursement costs of an anonymized health insurance rese...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000431104

    authors: Niklas C,Saar M,Berg B,Steiner K,Janssen M,Siemer S,Stöckle M,Ohlmann CH

    更新日期:2016-01-01 00:00:00

  • Comparison of the xenon-133 washout curve and radioactive microsphere distribution in the normal and hypoperfused dog kidney.

    abstract::Intrarenal blood flow distribution was measured in normal and hypoperfused dog kidneys by xenon-133 washout measurement in one kidney vary slightly in contrast to those done in different kidneys. In normal kidneys the ratio of compartment I (CI) flow to whole renal flow -- i.e., the CI fraction--as measured by the xen...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000280818

    authors: Huland H,Leichtweiss HP,Schröder H,Jeschkies R

    更新日期:1982-01-01 00:00:00

  • Predicting the risk of high-grade bladder cancer using noninvasive data.

    abstract:AIM:To examine the hypothesis that the risk of high-grade bladder cancer can be predicted using noninvasively obtained data. PATIENTS AND METHODS:We retrospectively analyzed the database of 431 patients that had transurethral resection of first-time bladder tumors between June 1998 and December 2009. Pre-operative par...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000328635

    authors: Shapur N,Pode D,Katz R,Shapiro A,Yutkin V,Pizov G,Appelbaum L,Zorn KC,Duvdevani M,Landau EH,Gofrit ON

    更新日期:2011-01-01 00:00:00

  • Alpha-fetoprotein-producing renal cell carcinoma.

    abstract::Alpha-fetoprotein (AFP) is recognized as a tumor marker of yolk sac tumors, liver cancer and some other cancers of the digestive organs. Renal cell carcinoma (RCC) producing AFP is a rare entity. A case of AFP-producing RCC with solitary bone metastasis, but without liver involvement, is reported. The stain specific t...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000050982

    authors: Poulakis V,Witzsch U,de Vries R,Becht E,Altmannsberger HM,Störkel S

    更新日期:2001-01-01 00:00:00

  • Repair of inguinal hernias using the mesh technique during extraperitoneal pelvic lymph node dissection.

    abstract:PURPOSE:This article describes our experience of using a totally extraperitoneal approach for endoscopic pelvic lymphadenectomy and inguinal hernia repair with the mesh technique in one procedure. MATERIALS AND METHODS:A total of 52 patients underwent modified pelvic lymph node dissection for the staging of prostate c...

    journal_title:Urologia internationalis

    pub_type: 杂志文章

    doi:10.1159/000050938

    authors: Stolzenburg JU,Pfeiffer H,Neuhaus J,Sommerfeld M,Dorschner W

    更新日期:2001-01-01 00:00:00