Abstract：
:Articular cartilage has limited capacity for regeneration and when damaged cannot be repaired with currently available metallic or synthetic implants. We aim to bioengineer a microfibre-reinforced hydrogel that can capture the zonal depth-dependent mechanical properties of native cartilage, and simultaneously support neo-cartilage formation. With this goal, a sophisticated bi-layered microfibre architecture, combining a densely distributed crossed fibre mat (superficial tangential zone, STZ) and a uniform box structure (middle and deep zone, MDZ), was successfully manufactured via melt electrospinning and combined with a gelatin-methacrylamide hydrogel. The inclusion of a thin STZ layer greatly increased the composite construct's peak modulus under both incongruent (3.2-fold) and congruent (2.1-fold) loading, as compared to hydrogels reinforced with only a uniform MDZ structure. Notably, the stress relaxation response of the bi-layered composite construct was comparable to the tested native cartilage tissue. Furthermore, similar production of sulphated glycosaminoglycans and collagen II was observed for the novel composite constructs cultured under mechanical conditioning w/o TGF-ß1 supplementation and in static conditions w/TGF-ß1 supplementation, which confirmed the capability of the novel composite construct to support neo-cartilage formation upon mechanical stimulation. To conclude, these results are an important step towards the design and manufacture of biomechanically competent implants for cartilage regeneration. STATEMENT OF SIGNIFICANCE: Damage to articular cartilage results in severe pain and joint disfunction that cannot be treated with currently available implants. This study presents a sophisticated bioengineered bi-layered fibre reinforced cell-laden hydrogel that can approximate the functional mechanical properties of native cartilage. For the first time, the importance of incorporating a viable superficial tangential zone (STZ) - like structure to improve the load-bearing properties of bioengineered constructs, particularly when in-congruent surfaces are compressed, is demonstrated. The present work also provides new insights for the development of implants that are able to promote and guide new cartilaginous tissue formation upon physiologically relevant mechanical stimulation.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Castilho M,Mouser V,Chen M,Malda J,Ito Kdoi
10.1016/j.actbio.2019.06.030subject
Has Abstractpub_date
2019-09-01 00:00:00pages
297-306eissn
1742-7061issn
1878-7568pii
S1742-7061(19)30443-Xjournal_volume
95pub_type
杂志文章abstract：:One of the challenges of using bioactive bone cements is adjusting their handling properties for clinical application. To resolve the poorer handling properties of bioactive bone cements we developed a novel bioactive bone cement containing a unique polymethylmethacrylate (PMMA) powder, termed SPD-PMMA (40 μm in diame...
journal_title：Acta biomaterialia
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更新日期：2016-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2010-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-01-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.10.008
更新日期：2008-05-01 00:00:00
abstract：:Vascular stiffening is associated with the prognosis of cardiovascular disease (CVD). Endothelial dysfunction, as shown by reduced vasodilation and increased vasoconstriction, not only affects vascular tone, but also accelerates the progression of CVD. However, the precise effect of vascular stiffening on endothelial ...
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pub_type： 杂志文章
doi：10.1016/j.actbio.2019.10.013
更新日期：2019-12-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2007.06.008
更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-03-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
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更新日期：2018-03-15 00:00:00
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更新日期：2012-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-02-01 00:00:00
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更新日期：2016-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
abstract：:Over the past decades, numerous tissue-engineered constructs have been investigated for the osteochondral repair. However, it still remains a challenge to regenerate the functionalized calcified layer. In this study, the potential of icariin (Ica) conjugated hyaluronic acid/collagen (Ica-HA/Col) hydrogel to promote th...
journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
abstract：:Tissue-engineered temporomandibular joint (TMJ) discs offer a viable treatment option for patients with severe joint internal derangement. To date, only a handful of TMJ tissue engineering studies have been carried out and all have incorporated the use of synthetic scaffold materials. These current scaffolds have show...
journal_title：Acta biomaterialia
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更新日期：2008-07-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2018-09-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.04.014
更新日期：2013-08-01 00:00:00
