Abstract：
:Antimicrobial peptides (AMPs), which are produced by multicellular organisms as a defense mechanism against competing pathogenic microbes, appear to be excellent candidates for the development of novel antimicrobial agents. Amphipathicity is traditionally believed to be crucial to the de novo design or systematic optimization of AMPs. In this study, we designed a series of short α-helical AMPs with imperfect amphipathicity to augment the arsenal of strategies and to gain further insights into their antimicrobial and hemolytic activity. These imperfectly amphipathic α-helical AMPs were designed by replacing the paired charged amino acid residues on the polar face of an amphipathic peptide with tryptophan residues on the basis of α-helical protein folding principles. PRW4, an imperfectly amphipathic α-helical AMP with hydrogen bonds formed by paired tryptophan residues, was observed to be more selective towards bacterial cells than toward human red blood cells. PRW4 was also effective against Gram-negative and Gram-positive bacteria, and fluorescence spectroscopy, flow cytometry, scanning electron microscopy and transmission electron microscopy indicated that PRW4 killed microbial cells by permeabilizing the cell membrane and damaging their membrane integrity. Therefore, disruptive amphipathicity has excellent potential for the rational design and optimization of AMPs with promising antimicrobial activities.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Zhu X,Dong N,Wang Z,Ma Z,Zhang L,Ma Q,Shan Adoi
10.1016/j.actbio.2013.08.043subject
Has Abstractpub_date
2014-01-01 00:00:00pages
244-57issue
1eissn
1742-7061issn
1878-7568pii
S1742-7061(13)00435-2journal_volume
10pub_type
杂志文章abstract：:A major challenge to the successful clinical application of bioengineered cartilage remains its integration to surrounding tissues upon implantation. One way to address this consists of generating biphasic constructs composed of articular cartilage formed in vitro on the top surface and integrated with the porous sub-...
journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2010-08-01 00:00:00
abstract：:Poloxamers, or pluronics, have been proposed as biomimetic substitutes for physiological gels. Concern regarding their ability to resist swelling under fluid flows has impeded their implementation. Using a combination of techniques including cryo-TEM and rapid X-ray imaging, we found that rapid flow rates stabilized t...
journal_title：Acta biomaterialia
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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abstract：:Small intestine submucosa (SIS) has emerged as one of a number of naturally derived extracellular matrix (ECM) biomaterials currently in clinical use. In addition to clinical applications, ECM materials form the basis for a variety of approaches within tissue engineering research. In our preliminary work it was found ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.08.024
更新日期：2014-12-01 00:00:00
abstract：UNLABELLED:Despite advances in polymeric nanoparticles (NPs) as effective delivery systems for anticancer drugs, rapid clearance from blood and poor penetration capacity in heterogeneous tumors still remain to be addressed. Here, a dual coating of poly (ethylene glycol)-poly (d,l-lactic acid) (PEG-PDLLA) and water-solu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.10.017
更新日期：2016-01-01 00:00:00
abstract：UNLABELLED:Biomimetic materials were developed to regulate stem cell behaviour. We have analyzed the influence of polycaprolactone (PCL) films, functionalized with adhesive peptides derived from fibronectin (pFibro) or bone sialoprotein (pBSP), on the response of murine multipotent C3H10T1/2 cells to bone morphogenetic...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.12.005
更新日期：2016-02-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.05.033
更新日期：2011-09-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.11.003
更新日期：2009-06-01 00:00:00
abstract：:Metal-binding peptides have attracted attention for their usefulness in biomineralization processes. In this work screening of high affinity peptides against ZnO was performed using a combinatorial library approach with a peptide array and computational analysis. The computationally assisted peptide screening and desi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.12.025
更新日期：2010-06-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.03.050
更新日期：2019-07-15 00:00:00
abstract：:Understanding the influence of a controlled spatial distribution of biological cues on cell activities can be useful to design "cell instructive" materials, able to control and guide the formation of engineered tissues in vivo and in vitro. To this purpose, biochemical and mechanical properties of the resulting biomat...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.12.050
更新日期：2010-07-01 00:00:00
abstract：:Myocardial infarction (MI) leads to the loss of cardiomyocytes, followed by left ventricular (LV) remodeling and cardiac dysfunction. The authors hypothesize that an elastic, biodegradable nanofibrous cardiac patch loaded with mesenchymal stem cells (MSC) could restrain LV remodeling and improve cardiac function after...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.02.030
更新日期：2014-06-01 00:00:00
abstract：:Controlling the release of recombinant adeno-associated virus (rAAV) vectors from biocompatible materials is a novel, attractive approach to increase the residence time and effectiveness of a gene carrier at a defined target site. Self-assembling peptides have an ability to form stable hydrogels and encapsulate cells ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.02.013
更新日期：2015-05-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.07.001
更新日期：2019-09-15 00:00:00
abstract：UNLABELLED:The irritation/discomfort associated with the use of contact lenses (CLs) is often related to the eyelid-lens friction. Although the use of such devices is widespread, the information about the influence of the lacrimal fluid biomolecules on the tribological behavior of the CLs hydrogels is scarce. In this w...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.08.011
更新日期：2015-10-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.11.018
更新日期：2009-05-01 00:00:00
abstract：:The present work is an amalgamation of computation and experimental approach to gain an insight into composition-structure-bioactivity relationships of alkali-free bioactive glasses in the CaO-MgO-SiO2-P2O5 system. The glasses have been designed in the diopside (CaO·MgO·2SiO2; Di)-tricalcium phosphate (3CaO·P2O5; TCP)...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.01.001
更新日期：2015-03-01 00:00:00
abstract：:Antibiotic resistance is a major challenge to the treatment of bacterial infections associated with medical devices and biomaterials. One important intrinsic mechanism of such resistance is the formation of persister cells that are phenotypic variants of microorganisms and highly tolerant to antibiotics. Recently, we ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.12.053
更新日期：2017-03-01 00:00:00
abstract：:Surface functionalization of teeth with fluoride-incorporated apatite layers displays great potential in treatments and prevention of dental disorders. In this study, we used a sintered hydroxyapatite (sHA) substrate as a model material of teeth, and established a rapid and area-specific coating technique of fluoride-...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.08.025
更新日期：2018-10-01 00:00:00
abstract：:Human pluripotent stem cells (hPSCs) represent a promising cell source for the development of β-cells for use in therapies for type 1 diabetes. Current culture approaches provide signals to mimic a temporal control of organogenesis to drive the differentiation towards β-cells. However, spatial control may represent an...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.06.032
更新日期：2019-09-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.12.003
更新日期：2010-06-01 00:00:00
abstract：:This paper presents a strain-mediated phenomenological corrosion model, based on the discrete finite element modelling method which was developed for use with the ANSYS Implicit finite element code. The corrosion model was calibrated from experimental data and used to simulate the corrosion performance of a WE43 magne...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.04.020
更新日期：2017-06-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.01.004
更新日期：2009-06-01 00:00:00
abstract：UNLABELLED:Injectable biomaterials have been evaluated as potential new therapies for myocardial infarction (MI) and heart failure. These materials have improved left ventricular (LV) geometry and ejection fraction, yet there remain concerns that biomaterial injection may create a substrate for arrhythmia. Since studie...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.08.004
更新日期：2015-10-01 00:00:00
abstract：UNLABELLED:Poly(3,4-ethylenedioxythiophenes) (PEDOT) have been extensively explored as materials for biomedical implants such as biosensors, tissue engineering scaffolds and microelectronic devices. Considerable effort has been made to incorporate biologically active molecules into the conducting polymer films in order...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.05.016
更新日期：2016-09-01 00:00:00
abstract：:The mechanical and electrical properties of polycaprolactone fumarate-polypyrrole (PCLF-PPy) scaffolds were studied under physiological conditions to evaluate their ability to maintain the material properties necessary for application as conductive nerve conduits. PC12 cells cultured on PCLF-PPy scaffolds were stimula...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.10.013
更新日期：2011-03-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.02.052
更新日期：2014-07-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.09.024
更新日期：2017-11-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.02.018
更新日期：2020-04-01 00:00:00
abstract：:We developed a novel titanium coating that has applications for preventing infection-related implant failures in dentistry and orthopedics. The coating incorporates an antimicrobial peptide, GL13K, derived from parotid secretory protein, which has been previously shown to be bactericidal and bacteriostatic in solution...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.06.017
更新日期：2013-09-01 00:00:00
