Development of osteopromotive poly (octamethylene citrate glycerophosphate) for enhanced bone regeneration.

Abstract:

:The design and development of bioactive materials that are inherently conducive for osteointegration and bone regeneration with tunable mechanical properties and degradation remains a challenge. Herein, we report the development of a new class of citrate-based materials with glycerophosphate salts, β-glycerophosphate disodium (β-GP-Na) and glycerophosphate calcium (GP-Ca), incorporated through a simple and convenient one-pot condensation reaction, which might address the above challenge in the search of suitable orthopedic biomaterials. Tensile strength of the resultant poly (octamethylene citrate glycerophosphate), POC-βGP-Na and POC-GP-Ca, was as high as 28.2 ± 2.44  MPa and 22.76 ± 1.06  MPa, respectively. The initial modulus ranged from 5.28 ± 0.56  MPa to 256.44 ± 22.88  MPa. The mechanical properties and degradation rate of POC-GP could be controlled by varying the type of salts, and the feeding ratio of salts introduced. Particularly, POC-GP-Ca demonstrated better cytocompatibility and the corresponding composite POC-GP-Ca/hydroxyapatite (HA) also elicited improved osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro, as compared to POC-βGP-Na/HA and POC/HA. The superior in-vivo performance of POC-GP-Ca/HA microparticle scaffolds in promoting bone regeneration over POC-βGP-Na/HA and POC/HA was further confirmed in a rabbit femoral condyle defect model. Taken together, the tunability of mechanical properties and degradation rates, together with the osteopromotive nature of POC-GP polymers make these materials, especially POC-GP-Ca well suited for bone tissue engineering applications. STATEMENT OF SIGNIFICANCE: The design and development of bioactive materials that are inherently conducive for osteointegration and bone regeneration with tunable mechanical properties and degradation remains a challenge. Herein, we report the development of a new class of citrate-based materials with glycerophosphate salts, β-glycerophosphate disodium (β-GPNa) and glycerophosphate calcium (GPCa), incorporated through a simple and convenient one-pot condensation reaction. The resultant POC-GP polymers showed significantly improved mechanical property and tunable degradation rate. Within the formulation investigated, POC-GPCa/HA composite further demonstrated better bioactivity in favoring osteogenic differentiation of hMSCs in vitro and promoted bone regeneration in rabbit femoral condyle defects. The development of POC-GP expands the repertoire of the well-recognized citrate-based biomaterials to meet the ever-increasing needs for functional biomaterials in tissue engineering and other biomedical applications.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

He Y,Li Q,Ma C,Xie D,Li L,Zhao Y,Shan D,Chomos SK,Dong C,Tierney JW,Sun L,Lu D,Gui L,Yang J

doi

10.1016/j.actbio.2019.03.050

subject

Has Abstract

pub_date

2019-07-15 00:00:00

pages

180-191

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(19)30226-0

journal_volume

93

pub_type

杂志文章
  • Dual-functional 3D-printed composite scaffold for inhibiting bacterial infection and promoting bone regeneration in infected bone defect models.

    abstract::Infection is one of the pivotal causes of nonunion in large bone defect after trauma or tumor resection. Three-dimensional (3D) composite scaffold with multifunctional-therapeutic properties offer many advantages over allogenic or xenogenic bone grafting for the restoration of challenging infected bone defects. In the...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.08.015

    authors: Yang Y,Chu L,Yang S,Zhang H,Qin L,Guillaume O,Eglin D,Richards RG,Tang T

    更新日期:2018-10-01 00:00:00

  • Lifetime assessment of atomic-layer-deposited Al2O3-Parylene C bilayer coating for neural interfaces using accelerated age testing and electrochemical characterization.

    abstract::The lifetime and stability of insulation are critical features for the reliable operation of an implantable neural interface device. A critical factor for an implanted insulation's performance is its barrier properties that limit access of biological fluids to the underlying device or metal electrode. Parylene C is a ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.10.031

    authors: Minnikanti S,Diao G,Pancrazio JJ,Xie X,Rieth L,Solzbacher F,Peixoto N

    更新日期:2014-02-01 00:00:00

  • Mechanism and kinetics of apatite formation on nanocrystalline TiO2 coatings: a quartz crystal microbalance study.

    abstract::Apatite (Ca5(PO4)3OH) has long been considered as an excellent biomaterial to promote bone repairs and implant. Apatite formation induced by negatively charged nanocrystalline TiO2 coatings soaked in simulated body fluid (SBF) was investigated using in situ quartz crystal microbalance (QCM), scanning electron microsco...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2007.10.003

    authors: Yang Z,Si S,Zeng X,Zhang C,Dai H

    更新日期:2008-05-01 00:00:00

  • Fibrin glue mesh fixation combined with mesenchymal stem cells or exosomes modulates the inflammatory reaction in a murine model of incisional hernia.

    abstract::Surgical meshes are effective and frequently used to reinforce soft tissues. Fibrin glue (FG) has been widely used for mesh fixation and is also considered an optimal vehicle for stem cell delivery. The aim of this preclinical study was to evaluate the therapeutic effect of MSCs and their exosomes combined with FG for...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.02.014

    authors: Blázquez R,Sánchez-Margallo FM,Álvarez V,Usón A,Marinaro F,Casado JG

    更新日期:2018-04-15 00:00:00

  • Thermally triggered injectable chitosan/silk fibroin/bioactive glass nanoparticle hydrogels for in-situ bone formation in rat calvarial bone defects.

    abstract::Copper-containing bioactive glass nanoparticles (Cu-BG NPs) with designed compositions and sizes were synthesized and incorporated into chitosan (CH)/silk fibroin (SF)/glycerophosphate (GP) composites to prepare injectable hydrogels for cell-free bone repair. The resulting Cu-BG/CH/SF/GP gels were found to exhibit wel...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.04.023

    authors: Wu J,Zheng K,Huang X,Liu J,Liu H,Boccaccini AR,Wan Y,Guo X,Shao Z

    更新日期:2019-06-01 00:00:00

  • Electrospun thermosensitive hydrogel scaffold for enhanced chondrogenesis of human mesenchymal stem cells.

    abstract::Hydrogels have shown great potential for cartilage tissue engineering applications due to their capability to encapsulate cells within biomimetic, 3-dimensional (3D) microenvironments. However, the multi-step fabrication process that is necessary to produce cell/scaffold constructs with defined dimensions, limits thei...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.11.020

    authors: Brunelle AR,Horner CB,Low K,Ico G,Nam J

    更新日期:2018-01-15 00:00:00

  • Tumor microenvironment targeting with dual stimuli-responsive nanoparticles based on small heat shock proteins for antitumor drug delivery.

    abstract::Tumour microenvironment (TME)-targeting nanoparticles (NPs) were developed based on Methanococcus jannaschii small heat shock proteins (Mj-sHSPs). Transactivator of transcription (TAT) were modified on the surface of Mj-sHSPs (T-HSPs) to enhance their cellular internalization ability (CIA), and a pH/enzyme dual sensit...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2020.07.031

    authors: Shi K,Wang Y,Zhou X,Gui H,Xu N,Wu S,He C,Zhao Z

    更新日期:2020-09-15 00:00:00

  • Mineralized nanofiber segments coupled with calcium-binding BMP-2 peptides for alveolar bone regeneration.

    abstract::Bone loss around tooth extraction sites can occur, thus making future placement of dental implants difficult. Alveolar bone regeneration can be guided by the application of a nanofibrous bone graft coupled with osteoinductive proteins/peptides, following tooth loss or tooth extraction. In the present study, we demonst...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.12.051

    authors: Boda SK,Almoshari Y,Wang H,Wang X,Reinhardt RA,Duan B,Wang D,Xie J

    更新日期:2019-02-01 00:00:00

  • Synthesis, mechanical and biological characterization of ionic doped carbonated hydroxyapatite/β-tricalcium phosphate mixtures.

    abstract::The influence of ionic substituents in calcium phosphates intended for bone and tooth replacement biomedical applications is an important research topic, owing to the essential roles played by trace elements in biological processes. The present study investigates the mechanical and biological evaluation of ionic doped...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.12.009

    authors: Kannan S,Vieira SI,Olhero SM,Torres PM,Pina S,da Cruz e Silva OA,Ferreira JM

    更新日期:2011-04-01 00:00:00

  • Strain specificity in antimicrobial activity of silver and copper nanoparticles.

    abstract::The antimicrobial properties of silver and copper nanoparticles were investigated using Escherichia coli (four strains), Bacillus subtilis and Staphylococcus aureus (three strains). The average sizes of the silver and copper nanoparticles were 3 nm and 9 nm, respectively, as determined through transmission electron mi...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2007.11.006

    authors: Ruparelia JP,Chatterjee AK,Duttagupta SP,Mukherji S

    更新日期:2008-05-01 00:00:00

  • bFGF-containing electrospun gelatin scaffolds with controlled nano-architectural features for directed angiogenesis.

    abstract::Current therapeutic angiogenesis strategies are focused on the development of biologically responsive scaffolds that can deliver multiple angiogenic cytokines and/or cells in ischemic regions. Herein, we report on a novel electrospinning approach to fabricate cytokine-containing nanofibrous scaffolds with tunable arch...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.12.008

    authors: Montero RB,Vial X,Nguyen DT,Farhand S,Reardon M,Pham SM,Tsechpenakis G,Andreopoulos FM

    更新日期:2012-05-01 00:00:00

  • Immunogenicity in xenogeneic scaffold generation: antigen removal vs. decellularization.

    abstract::Decades of research have been undertaken towards the goal of tissue engineering using xenogeneic scaffolds. The primary advantages associated with use of xenogeneic tissue-derived scaffolds for in vitro development of replacement tissues and organs stem from the inherent extracellular matrix (ECM) composition and arch...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章,评审

    doi:10.1016/j.actbio.2014.01.028

    authors: Wong ML,Griffiths LG

    更新日期:2014-05-01 00:00:00

  • Injectable, self-healable zwitterionic cryogels with sustained microRNA - cerium oxide nanoparticle release promote accelerated wound healing.

    abstract::Diabetics are prone to chronic wounds that have slower healing, and methods of accelerating the wound closure and to ensure protection from infections are critically needed. MicroRNA-146a gets dysregulated in diabetic wounds and injection of this microRNA combined with reactive oxygen species-scavenging cerium oxide n...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.11.014

    authors: Sener G,Hilton SA,Osmond MJ,Zgheib C,Newsom JP,Dewberry L,Singh S,Sakthivel TS,Seal S,Liechty KW,Krebs MD

    更新日期:2020-01-01 00:00:00

  • Screening of rat mesenchymal stem cell behaviour on polydimethylsiloxane stiffness gradients.

    abstract::Substrate stiffness is emerging as an effective tool for the regulation of cell behaviours such as locomotion, proliferation and differentiation. In order to explore the potential application of this biophysical tool, material platforms displaying lateral and continuously graded stiffness are advantageous since they a...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.09.030

    authors: Wang PY,Tsai WB,Voelcker NH

    更新日期:2012-02-01 00:00:00

  • Study and evaluation of mechanisms of dual targeting drug delivery system with tumor microenvironment assays compared with normal assays.

    abstract::A dual targeting delivery system was developed to completely conquer the two barriers that glioma treatment faces: the blood-brain barrier (BBB) and the brain-glioma barrier. Recently, a system comprising AS1411 aptamer (for glioma targeting) and TGN peptide (for BBB targeting) modified nanoparticles (AsTNPs) was deve...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.11.003

    authors: Gao H,Yang Z,Zhang S,Pang Z,Liu Q,Jiang X

    更新日期:2014-02-01 00:00:00

  • Multilayer mediated forward and patterned siRNA transfection using linear-PEI at extended N/P ratios.

    abstract::Gene delivery from a substrate depends, in part, on the vector-nucleic acid complex that is bound to the surface and the cell adhesive properties of the surface. Here, we present a method to deliver patterns of small interfering RNA (siRNA) that capitalize on a forward transfection method (transfection by introducing ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.01.004

    authors: Mehrotra S,Lee I,Chan C

    更新日期:2009-06-01 00:00:00

  • Electrospun fibrous scaffolds with continuous gradations in mineral contents and biological cues for manipulating cellular behaviors.

    abstract::Challenges remain in the generation of heterogeneous tissues and the repairing of interfacial tissue between soft and hard tissues. The development of tissue engineering scaffolds with gradients in composition, structure, mechanical and chemical properties is essential to modulate cellular behaviors in a graded way an...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.01.003

    authors: Zou B,Liu Y,Luo X,Chen F,Guo X,Li X

    更新日期:2012-04-01 00:00:00

  • Accelerated wound closure of pressure ulcers in aged mice by chitosan scaffolds with and without bFGF.

    abstract::Pressure ulcers are a significant healthcare concern, especially for elderly populations. Our work served to ameliorate the chronicity of these ulcers by addressing ischemia-reperfusion injury mediated by neutrophils and the concomitant loss of vasculature in these wounds. To this end, chitosan scaffolds loaded with b...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.03.002

    authors: Park CJ,Clark SG,Lichtensteiger CA,Jamison RD,Johnson AJ

    更新日期:2009-07-01 00:00:00

  • Enzymatic conjugation of a bioactive peptide into an injectable hyaluronic acid-tyramine hydrogel system to promote the formation of functional vasculature.

    abstract::In this study, one-step enzyme-mediated preparation of a multi-functional injectable hyaluronic-acid-based hydrogel system is reported. Hydrogel was formed through the in situ coupling of phenol moieties by horseradish peroxidase (HRP) and hydrogen peroxide (H2O2), and bioactive peptides were simultaneously conjugated...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.02.022

    authors: Wang LS,Lee F,Lim J,Du C,Wan AC,Lee SS,Kurisawa M

    更新日期:2014-06-01 00:00:00

  • A novel hydrogel-collagen composite improves functionality of an injectable extracellular matrix.

    abstract::Cellular transplantation is now closer to becoming a practical clinical strategy to repair, regenerate or restore the function of skin, muscle, nerves and pancreatic islets. In this study we sought to develop a simple injectable collagen matrix that would preserve the normal cellular organization of skin cells. Three ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.04.024

    authors: Hartwell R,Leung V,Chavez-Munoz C,Nabai L,Yang H,Ko F,Ghahary A

    更新日期:2011-08-01 00:00:00

  • Hydroxyapatite coating affects the Wnt signaling pathway during peri-implant healing in vivo.

    abstract::Owing to its bio- and osteoconductivity, hydroxyapatite (HA) is a widely used implant material, but its osteogenic properties are only partly evaluated in vitro and in vivo. The present study focused on bone healing adjacent to HA-coated titanium (Ti) implants, with or without incorporated lithium ions (Li(+)). Specia...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.12.012

    authors: Thorfve A,Lindahl C,Xia W,Igawa K,Lindahl A,Thomsen P,Palmquist A,Tengvall P

    更新日期:2014-03-01 00:00:00

  • Crosslinked collagen hydrogels as corneal implants: effects of sterically bulky vs. non-bulky carbodiimides as crosslinkers.

    abstract::We have previously shown that recombinant human collagen can be crosslinked with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) to fabricate transparent hydrogels possessing the shape and dimensions of the human cornea. These corneal implants have been tested in a Phase I human clinical study. Although these hyd...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.04.014

    authors: Ahn JI,Kuffova L,Merrett K,Mitra D,Forrester JV,Li F,Griffith M

    更新日期:2013-08-01 00:00:00

  • Characterization of polyethylene wear particle: The impact of methodology.

    abstract::Due to the prevalence of problems caused by wear particles, the reduced durability of total joint replacements is well documented. The characterization of wear debris enables the size and morphology of these wear particles to be measured and provides an assessment of the biological response in vivo. However, the impac...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.07.039

    authors: Schröder C,Reinders J,Zietz C,Utzschneider S,Bader R,Kretzer JP

    更新日期:2013-12-01 00:00:00

  • Monolithic and assembled polymer-ceramic composites for bone regeneration.

    abstract::The rationale for the use of polymer-ceramic composites for bone regeneration stems from the natural composition of bone, with collagen type I and biological apatite as the main organic and inorganic constituents, respectively. In the present study composite materials of PolyActive™ (PA), a poly(ethylene oxide terepht...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.10.044

    authors: Nandakumar A,Cruz C,Mentink A,Tahmasebi Birgani Z,Moroni L,van Blitterswijk C,Habibovic P

    更新日期:2013-03-01 00:00:00

  • Quantitative ultrasound imaging of cell-laden hydrogels and printed constructs.

    abstract::In the present work we have revisited the application of quantitative ultrasound imaging (QUI) to cellular hydrogels, by using the reference phantom method (RPM) in combination with a local attenuation compensation algorithm. The investigated biological samples consisted of cell-laden collagen hydrogels with PC12 neur...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.04.055

    authors: Ruland A,Gilmore KJ,Daikuara LY,Fay CD,Yue Z,Wallace GG

    更新日期:2019-06-01 00:00:00

  • Micropillar arrays as potential drug screens: Inhibition of micropillar-mediated activation of the FAK-Src-paxillin signaling pathway by the CK2 inhibitor CX-4945.

    abstract:UNLABELLED:Here, we demonstrate the possible applications of micropillar arrays in screening anti-metastasis drugs. Human lung adenocarcinoma A549 cells incubated in multiwell plates containing micropillars exhibited markedly different physical/biochemical behavior depending on pillar dimensions. In particular, A549 ce...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2015.08.041

    authors: Kim J,Choi WJ,Moon SH,Jung J,Park JK,Kim SH,Lee JO

    更新日期:2015-11-01 00:00:00

  • Gadolinium-based bimodal probes to enhance T1-Weighted magnetic resonance/optical imaging.

    abstract::Gd3+-based contrast agents have been extensively used for signal enhancement of T1-weighted magnetic resonance imaging (MRI) due to the large magnetic moment and long electron spin relaxation time of the paramagnetic Gd3+ ion. The key requisites for the development of Gd3+-based contrast agents are their relaxivities ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章,评审

    doi:10.1016/j.actbio.2020.03.047

    authors: Yang CT,Hattiholi A,Selvan ST,Yan SX,Fang WW,Chandrasekharan P,Koteswaraiah P,Herold CJ,Gulyás B,Aw SE,He T,Ng DCE,Padmanabhan P

    更新日期:2020-07-01 00:00:00

  • Core-shell PVA/gelatin electrospun nanofibers promote human umbilical vein endothelial cell and smooth muscle cell proliferation and migration.

    abstract:UNLABELLED:Cardiovascular disease is the leading cause of death in the world. In this study, coaxial electrospinning is employed to fabricate fibers in a core-shell structure with polyvinyl alcohol (PVA) in the core and gelatin in the shell for evaluation as a potential vascular tissue engineering construct. PVA, a syn...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2015.08.044

    authors: Merkle VM,Tran PL,Hutchinson M,Ammann KR,DeCook K,Wu X,Slepian MJ

    更新日期:2015-11-01 00:00:00

  • Self-assembling peptide amphiphile nanofiber matrices for cell entrapment.

    abstract::We have developed a class of peptide amphiphile (PA) molecules that self-assemble into three-dimensional nanofiber networks under physiological conditions in the presence of polyvalent metal ions. The assembly can be triggered by adding PA solutions to cell culture media or other synthetic physiological fluids contain...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2005.04.002

    authors: Beniash E,Hartgerink JD,Storrie H,Stendahl JC,Stupp SI

    更新日期:2005-07-01 00:00:00

  • Influence of nanostructural environment and fluid flow on osteoblast-like cell behavior: a model for cell-mechanics studies.

    abstract::Introducing nanoroughness on various biomaterials has been shown to profoundly effect cell-material interactions. Similarly, physical forces act on a diverse array of cells and tissues. Particularly in bone, the tissue experiences compressive or tensile forces resulting in fluid shear stress. The current study aimed t...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.02.011

    authors: Prodanov L,Semeins CM,van Loon JJ,te Riet J,Jansen JA,Klein-Nulend J,Walboomers XF

    更新日期:2013-05-01 00:00:00