Abstract:
:Oleuropein (OP) is a bioactive compound derived from plants of the genus Oleaceae exhibiting antitumor properties in several human cancers, including non-small-cell lung cancer (NSCLC). Recent evidence suggests that OP has proapoptotic effects on NSCLC cells via the mitochondrial apoptotic pathway. However, the exact molecular mechanisms behind the apoptogenic action of OP in NSCLC are still largely unknown. Glyoxalase 2 (Glo2) is an ancient enzyme belonging to the glyoxalase system involved in the detoxification of glycolysis-derived methylglyoxal. However, emerging evidence suggests that Glo2 may have also nonenzymatic roles in some malignant cells. In the present study, we evaluated whether and how Glo2 participated in the proapoptotic effects of OP in NSCLC A549 cells. Our results indicate that OP is able to induce apoptosis in A549 cells through the upregulation of mitochondrial Glo2 (mGlo2), mediated by the superoxide anion and Akt signaling pathway. Moreover, our data shows that the proapoptotic role of mGlo2, observed following OP exposure, occurs via the interaction of mGlo2 with the proapoptotic Bax protein. Conversely, OP does not alter the behavior of nonmalignant human BEAS-2B cells or mGlo2 expression, thus suggesting a specific anticancer role for this bioactive compound in NSCLC. Our data identify a novel pathway through which OP exerts a proapoptotic effect in NSCLC and suggest, for the first time, a novel, nonenzymatic antiapoptotic role for this ancient enzyme in NSCLC.
journal_name
Oxid Med Cell Longevjournal_title
Oxidative medicine and cellular longevityauthors
Antognelli C,Frosini R,Santolla MF,Peirce MJ,Talesa VNdoi
10.1155/2019/8576961subject
Has Abstractpub_date
2019-07-22 00:00:00pages
8576961eissn
1942-0900issn
1942-0994journal_volume
2019pub_type
杂志文章abstract::Some evidence indicated that chemoresistance associates with the acquisition of cancer stem-like properties. Recent studies suggested that chemokines can promote the chemoresistance and stem cell properties in various cancer cells, while the underling mechanism is still not completely illustrated. In our study, we fou...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/5874127
更新日期:2016-01-01 00:00:00
abstract:Objective:Cholesterol oxidation products have an established proatherogenic and cytotoxic effect. An increased exposure to these substances may be associated with the development of atherosclerosis and cancers. Relatively little, though, is known about the effect of phytosterol oxidation products, although phytosterols...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/1983975
更新日期:2019-11-15 00:00:00
abstract::Oxidative stress plays a crucial role in the pathogenesis of acute pancreatitis (AP). Isoliquiritigenin (ISL) is a flavonoid monomer with confirmed antioxidant activity. However, the specific effects of ISL on AP have not been determined. In this study, we aimed to investigate the protective effect of ISL on AP using ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/7161592
更新日期:2018-04-26 00:00:00
abstract::Barley grass powder is the best functional food that provides nutrition and eliminates toxins from cells in human beings; however, its functional ingredients have played an important role as health benefit. In order to better cognize the preventive and therapeutic role of barley grass for chronic diseases, we carried ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2018/3232080
更新日期:2018-04-04 00:00:00
abstract::Hypoxic-ischemic encephalopathy (HIE) is detrimental to newborns and is associated with high mortality and poor prognosis. Thus, the primary aim of the present study was to determine whether glycine could (1) attenuate HIE injury in rats and hypoxic stress in PC12 cells and (2) downregulate mitochondria-mediated autop...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/4248529
更新日期:2019-02-06 00:00:00
abstract::Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery tightened for 6 h the...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/6012636
更新日期:2018-04-02 00:00:00
abstract::Cardiolipin interacts with many proteins of the mitochondrial inner membrane and, together with cytochrome C and creatine kinase, activates them. It can be considered as an integrating factor for components of the mitochondrial respiratory chain, which provides for an efficient transfer of electrons and protons. The m...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/2901057
更新日期:2019-10-24 00:00:00
abstract::Ulcerative colitis (UC) is a common inflammatory bowel disease that can destroy the integrity of the colon and increase the risk of colorectal cancer. Oxidative stress is one of the critical pathogenic factors for UC, further impairing the entire affected colon. The Nrf2-ARE signaling pathway plays an important role i...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/3271617
更新日期:2018-05-20 00:00:00
abstract::Oxidative stress reflects an imbalance between oxidants and antioxidants in favor of the oxidants capable of evoking tissue damage. Like hydrogen sulfide (H2S) and nitric oxide (NO), carbon monoxide (CO) is an endogenous gaseous mediator recently implicated in the physiology of the gastrointestinal (GI) tract. CO is p...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2020/5083876
更新日期:2020-04-15 00:00:00
abstract::Aging leads to a number of disadvantageous changes in the cardiovascular system. Deterioration of vascular homoeostasis with increase in oxidative stress, chronic low-grade inflammation, and impaired nitric oxide bioavailability results in endothelial dysfunction, increased vascular stiffness, and compromised arterial...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2015/658264
更新日期:2015-01-01 00:00:00
abstract::Introduction. Aerodigestive squamous cell carcinomas (ASCC) constitute a major source of global cancer deaths. Patients typically present with advanced, incurable disease, so new means of detecting early disease are a research priority. Metabolite quantitation is amenable to point-of-care analysis and can be performed...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2020/2930347
更新日期:2020-02-21 00:00:00
abstract::The skin covers the outer surface of the body, so the epidermal keratinocytes within it are susceptible to reactive oxygen species (ROS) generated by environmental pollutants such as benzo(a)pyrene (BaP), a potent activator of aryl hydrocarbon receptor (AHR). Antioxidant activity is generally mediated by the nuclear f...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/9524657
更新日期:2018-02-14 00:00:00
abstract::Hypoxia-inducible gene domain family member 1A (Higd1a) has recently been reported to protect cells from hypoxia by helping to maintain normal mitochondrial function. The potential induction of Higd1a under high-fat exposure and whether it could protect cells from oxidative stress attracted our attention. Initially, 0...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/6051262
更新日期:2019-04-08 00:00:00
abstract::Non-thermal plasma (NTP), an ionized gas generated at ambient pressure and temperature, has been an emerging technology for medical applications. Through controlled delivery of reactive oxygen and nitrogen species (ROS/RNS), NTP can elicit hormetic cellular responses, thus stimulating broad therapeutic effects. To ena...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/9750206
更新日期:2020-12-03 00:00:00
abstract::Background. The combination antiretroviral therapy (cART) increases the oxidative stress in HIV-infected people, which in turn favors the onset and aggravation of non-AIDS comorbidities, a common situation affecting these individuals. We aimed to evaluate the influence of cART initiation on oxidative stress parameters...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2017/9834803
更新日期:2017-01-01 00:00:00
abstract::Hyperthyroidism is an endocrine disorder characterized by excessive secretion of thyroid hormones T3 and T4. Thyroid hormones exert pleiotropic actions on numerous tissues and induce an overall increase in metabolism, with an increase in energy demand and oxygen consumption. Therefore, the purpose of this study was to...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/7471890
更新日期:2019-06-02 00:00:00
abstract::Proteomic studies have suggested a biochemical interaction between α subunit of the large conductance, voltage- and Ca2+-activated potassium channel (BKCaα), and annexin A5 (ANXA5), which we verify here by coimmunoprecipitation and double labelling immunocytochemistry. The observation that annexin is flipped to the ou...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/1607092
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Chronic alcohol-related liver disease (ALD) is mediated by insulin resistance, mitochondrial dysfunction, inflammation, oxidative stress, and DNA damage. Recent studies suggest that dysregulated lipid metabolism with accumulation of ceramides, together with ER stress potentiate hepatic insulin resistance and...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2012/479348
更新日期:2012-01-01 00:00:00
abstract::Neutrophils play a critical role in the initiation and maintenance of intestinal inflammation. However, conventional neutrophil-targeted therapies can impair normal host defense. Tanshinone IIA has been recently revealed to act directly on neutrophils. Hence, we aimed at investigating whether Tanshinone IIA can protec...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/7916763
更新日期:2016-01-01 00:00:00
abstract::Ascorbate requiring Fe2+/2-oxoglutarate-dependent dioxygenases located in the nucleoplasm have been shown to participate in epigenetic regulation of gene expression via histone and DNA demethylation. Transport of dehydroascorbic acid is impaired in the endomembranes of fibroblasts from arterial tortuosity syndrome (AT...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/8156592
更新日期:2019-01-13 00:00:00
abstract::Neural stem/progenitor cells (NSPCs) are self-renewing, multipotent cells located in the embryonic and adult central nervous system (CNS). Extensive preclinical and clinical studies have shed light on the potential of stem cell replacement therapy for various neurodegenerative diseases. The key prerequisite for the su...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/5963037
更新日期:2020-04-25 00:00:00
abstract::Based on the role of oxidative stress in the pathophysiological mechanisms of sepsis and the importance of PCT as a clinically applicable biomarker for early detection of inflammatory response initiation, we aimed this study at examining the correlation between PCT levels and oxidative stress parameters (prooxidants a...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/5147364
更新日期:2020-09-04 00:00:00
abstract::The placenta plays a vital role in fetal development during pregnancy. Dysfunction of the placenta can be caused by oxidative stress and can lead to abnormal fetal development. Preventing oxidative stress of the placenta is thus an important measure to ensure positive birth outcomes. Research shows that tryptophan and...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2018/1054797
更新日期:2018-07-22 00:00:00
abstract::Chronic unpredictable mild stress (CUMS) induces hippocampal oxidative stress. H2S functions as a neuroprotectant against oxidative stress in brain. We have previously shown the upregulatory effect of H2S on BDNF protein expression in the hippocampus of rats. Therefore, we hypothesized that H2S prevents CUMS-generated...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2016/2153745
更新日期:2016-01-01 00:00:00
abstract::Cellular senescence is a state of irreversible cell cycle arrest in response to an array of cellular stresses. An important role for senescence has been shown for a number of pathophysiological conditions that include cardiovascular disease, pulmonary fibrosis, and diseases of the skin. However, whether senescence con...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2020/2583601
更新日期:2020-03-09 00:00:00
abstract:Methods:The expression of CD86, CD206, and HIF-1α in the gastric mucosa was evaluated through immunohistochemistry. RAW 264.7 cells were cocultured with H. pylori at various multiplicities of infection (MOIs), and iNOS, CD86, Arg-1, CD206, and HIF-1α expression was detected by Western blot, PCR, and ELISA analyses. ROS...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2020/5281795
更新日期:2020-12-08 00:00:00
abstract::Age-related hearing loss (ARHL), the progressive loss of hearing associated with aging, is the most common sensory disorder in the elderly population. The pathology of ARHL includes the hair cells of the organ of Corti, stria vascularis, and afferent spiral ganglion neurons as well as the central auditory pathways. Ma...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2014/582849
更新日期:2014-01-01 00:00:00
abstract::Oxidative stress has been proven as one of the most critical regulatory mechanisms involved in fine Particulate Matter- (PM2.5-) mediated toxicity. For a better understanding of the underlying mechanisms that enable oxidative stress to participate in PM2.5-induced toxic effects, the current study explored the effects ...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2019/2492368
更新日期:2019-12-07 00:00:00
abstract::Virulence of enterohemorrhagic Escherichia coli (EHEC) strains depends on production of Shiga toxins. These toxins are encoded in genomes of lambdoid bacteriophages (Shiga toxin-converting phages), present in EHEC cells as prophages. The genes coding for Shiga toxins are silent in lysogenic bacteria, and prophage indu...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章,评审
doi:10.1155/2016/3578368
更新日期:2016-01-01 00:00:00
abstract::Platelet-rich plasma (PRP) has grown as an attractive biologic instrument in regenerative medicine for its powerful healing properties. It is considered as a source of growth factors that may induce tissue repairing and improve fibrosis. This product has proven its efficacy in multiple studies, but its effect on cispl...
journal_title:Oxidative medicine and cellular longevity
pub_type: 杂志文章
doi:10.1155/2018/9658230
更新日期:2018-07-18 00:00:00