Metrizamide density gradients for separation of different developmental stages of malarial parasites.

Abstract:

:Density gradients with metrizamide, a tri-iodinated benzamido derivative of glucose, have been used to separate erythrocytes infected with three species of murine plasmodia. Uninfected erythrocytes separated well from erythrocytes containing parasites in different developmental stages. With metrizamide solutions, the densities required for isopyknic separation can be obtained without hypertonicity or high viscosity, and the viability and metabolism of parasites and erythrocytes are not detectably modified by exposure to these solutions. This type of separation has many possible applications to biochemical and immunological investigations.

authors

Eugui EM,Allison AC

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

181-7

eissn

0042-9686

issn

1564-0604

journal_volume

57 Suppl 1

pub_type

杂志文章
  • The natural history of HIV-1 and HIV-2 infections in adults in Africa: a literature review.

    abstract::About 30 million people in Africa are estimated to be living with human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS), yet data about the natural history of infection on the continent are sparse. We reviewed the literature on the natural history of HIV-1 and HIV-2 infections among African adult...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章,评审

    doi:

    authors: Jaffar S,Grant AD,Whitworth J,Smith PG,Whittle H

    更新日期:2004-06-01 00:00:00

  • Experimental infection of animals with influenzavirus types A and B.

    abstract::The knowledge that domestic cats were susceptible to infection with freshly isolated A/Hong Kong/68 influenzavirus led to studies on the susceptibility of some other animal species to this virus, as well as to studies on the ability of egg-passaged Hong Kong virus and an Asian virus to infect cats. The ability of a re...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Paniker CK,Nair CM

    更新日期:1972-01-01 00:00:00

  • THE FREQUENCY OF VENEREAL DISEASE AMONG SEAFARERS.

    abstract::Venereal diseases are known to occur most frequently in the age-groups of greatest sexual activity and more frequently among people in certain occupations (e.g., migrant labourers, military personnel and seafarers) than among the general population. The Brussels Agreement of 1924 and the great improvements in conditio...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: IDSOE O,GUTHE T

    更新日期:1963-01-01 00:00:00

  • Nomenclature for T-cell receptor (TCR) gene segments of the immune system. WHO-IUIS Nomenclature Sub-Committee on TCR Designation.

    abstract::The recommended procedures and criteria for T-cell receptor (TCR) designations are described. The officially adopted designations are for the TCR A, B, D and G loci and for V, D, J and C segments. ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors:

    更新日期:1993-01-01 00:00:00

  • Preparation of lyophilized rabies street virus material from infective submaxillary glands for challenge purposes.

    abstract::A method is described for the preparation of lyophilized rabies street virus material from infected submaxillary glands for challenge purposes. The lyophilized material gives consistent and reproducible results on intracerebral or intramuscular titration over long periods. ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: VEERARAGHAVAN N

    更新日期:1957-01-01 00:00:00

  • A cross-sectional community study of post-traumatic stress disorder and social support in Lao People's Democratic Republic.

    abstract:OBJECTIVE:To estimate post-traumatic stress disorder (PTSD) symptoms in those injured and not injured by landmines or unexploded ordnance (UXO) in rural Lao People's Democratic Republic and to determine whether the perception of social support was associated with PTSD symptom severity. METHODS:A community survey was c...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.12.115311

    authors: Southivong B,Ichikawa M,Nakahara S,Southivong C

    更新日期:2013-10-01 00:00:00

  • Cholinesterase inhibition by organophosphorus compounds and its clinical effects.

    abstract::The clinical manifestations of acute poisoning by organophosphorus compounds in man are in accord with, initially, the stimulation and, later, the blocking of cholinergic transmission due to acetylcholinesterase inhibition. The manifestations involve mainly the para-sympathetic nerves, the neuromuscular junctions, and...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章,评审

    doi:

    authors: Namba T

    更新日期:1971-01-01 00:00:00

  • Monkeypox virus: ecology and public health significance.

    abstract::Recent investigations have revealed that monkeypox virus infections occur with a high prevalence in several species of squirrels belonging to the genus Funisciurus, less frequently in squirrels of the genus Heliosciurus, and rarely in forest-dwelling primates. These squirrels commonly inhabit the secondary forests aro...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Khodakevich L,Jezek Z,Messinger D

    更新日期:1988-01-01 00:00:00

  • The dose-response lines for diphtheria toxoid fractions precipitated at various concentrations of ammonium sulfate.

    abstract::Three diphtheria toxoid preparations, fractionated at various concentrations of ammonium sulfate, having various grades of purity, and showing striking differences in immunizing potency when compared at the same Lf dose, were examined for similarity of the effective constituents in the fractions. No evidence of deviat...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: KUROKAWA M,NAKANO T,KONDO H

    更新日期:1954-01-01 00:00:00

  • Poliomyelitis in Japan during the period 1962-68 after the introduction of mass vaccination with Sabin vaccine.

    abstract::After the mass vaccination of children of susceptible age groups in Japan in 1961, the incidence of poliomyelitis decreased markedly. From 1962 to 1968 a total of 659 paralytic cases were officially notified. Detailed investigations of 626 cases reported to the Poliomyelitis Surveillance Committee revealed that only 1...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Takatsu T,Tagaya I,Hirayama M

    更新日期:1973-01-01 00:00:00

  • Research synthesis and dissemination as a bridge to knowledge management: the Cochrane Collaboration.

    abstract::In the current information age, research synthesis is a particularly useful tool for keeping track of scientific research and making sense of the large volumes of frequently conflicting data derived from primary studies. The Cochrane Collaboration is a global initiative "to help people make well-informed decisions abo...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章,评审

    doi:

    authors: Volmink J,Siegfried N,Robertson K,Gülmezoglu AM

    更新日期:2004-10-01 00:00:00

  • How important are airfreight rates and vaccine packaging in cost-saving efforts for the Expanded Programme on Immunization?

    abstract::Vaccines constitute the single most important cost factor in the Expanded Programme on Immunization (EPI) in Mozambique and in view of future new disease-control initiatives, the proportional expenditure on vaccines will only increase. Airfreight may contribute up to at least 25% of the total cost of delivered vaccine...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Schreuder B,Arentsen H,Matosse M

    更新日期:1997-01-01 00:00:00

  • The uptake of tritiated hycanthone by male and female Schistosoma mansoni worms and distribution of the drug in plasma and whole blood of mice following a single intramuscular injection.

    abstract::Mice infected with Schistosoma mansoni were given an intramuscular injection of a single 80 mg/kg dose of randomly tritiated hycanthone. The uptake of the drug in male and female worms, as well as its concentration in the red blood cells and plasma of the mice, was followed for a period of 24 hours.Blood and plasma dr...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Yarinsky A,Hernandez P,Dennis EW

    更新日期:1970-01-01 00:00:00

  • SEGREGATION OF GENETIC FACTORS DURING RECOMBINATION IN VIBRIO CHOLERAE, STRAIN 162.

    abstract::In this paper an analysis of genetic recombinants derived from crosses between two mutant stocks of Vibrio cholerae, strain 162, differing from one another in nutritional requirements and other characters, is presented. It is shown that parent strains possessing a fertility factor (designated as the P factor) function...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: BHASKARAN K

    更新日期:1964-01-01 00:00:00

  • Future cooperation in the field of rheumatoid arthritis and related diseases.

    abstract::This Memorandum discusses two areas of research into rheumatic disease in which it is considered international cooperation would be valuable. These are: (a) standardization and cooperative evaluation of immunological tests used in diagnosis, clinico-pathological assessment, and epidemiological work; and (b) standardiz...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors:

    更新日期:1974-01-01 00:00:00

  • A simple system for the assessment of trachoma and its complications.

    abstract::A simple grading system for trachoma, based on the presence or absence of five selected "key" signs, has been developed. The method was tested in the field and showed good observer agreement, the most critical point being the identification of severe cases of the disease. It is expected that the system will facilitate...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Thylefors B,Dawson CR,Jones BR,West SK,Taylor HR

    更新日期:1987-01-01 00:00:00

  • Health transition in Africa: practical policy proposals for primary care.

    abstract::Sub-Saharan Africa is undergoing health transition as increased globalization and accompanying urbanization are causing a double burden of communicable and noncommunicable diseases. Rates of communicable diseases such as HIV/AIDS, tuberculosis and malaria in Africa are the highest in the world. The impact of noncommun...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.10.077891

    authors: Maher D,Smeeth L,Sekajugo J

    更新日期:2010-12-01 00:00:00

  • Observations on phlebotomine sandflies in Iran.

    abstract::A survey of the sandfly population of Iran was conducted in the interests of providing further knowledge of the biology of these vectors of leishmaniasis, sandfly fever and kalaazar. The search for specimens was carried out in the Tabriz, Sarab, Caspian Sea, Teheran and Kazerun areas, both inside buildings and in natu...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: LEWIS DJ,MESGHALI A,DJANBAKHSH B

    更新日期:1961-01-01 00:00:00

  • Reforms for financial protection schemes towards universal health coverage, Senegal.

    abstract::Advancing the public health insurance system is one of the key strategies of the Senegalese government for achieving universal health coverage. In 2013, the government launched a universal health financial protection programme, la Couverture Maladie Universelle. One of the programme's aims was to establish a community...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.19.239665

    authors: Daff BM,Diouf S,Diop ESM,Mano Y,Nakamura R,Sy MM,Tobe M,Togawa S,Ngom M

    更新日期:2020-02-01 00:00:00

  • Natural Brucella infection in Argentine wild foxes.

    abstract::In the course of an investigation in 1962-64 into the natural occurrence of brucellosis among grey foxes in Argentina, agglutination tests were performed on 728 sera of the foxes Dusicyon gymnocercus antiquus and D. griseus griseus, captured in the provinces of Buenos Aires and Rio Negro. Agglutination titres of from ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Szyfres B,González Tomé J

    更新日期:1966-01-01 00:00:00

  • Global cost of correcting vision impairment from uncorrected refractive error.

    abstract:OBJECTIVE:To estimate the global cost of establishing and operating the educational and refractive care facilities required to provide care to all individuals who currently have vision impairment resulting from uncorrected refractive error (URE). METHODS:The global cost of correcting URE was estimated using data on th...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.12.104034

    authors: Fricke TR,Holden BA,Wilson DA,Schlenther G,Naidoo KS,Resnikoff S,Frick KD

    更新日期:2012-10-01 00:00:00

  • Cost and effectiveness analysis of immunization service delivery support in Andhra Pradesh, India.

    abstract::The immunization service delivery support (ISDS) model was initiated in Andhra Pradesh, India, in November 2003 with the aim of strengthening immunization services through supportive supervision. The ISDS model involves a well-established supervision system built upon the existing health infrastructure. The objectives...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/blt.06.039495

    authors: Suraratdecha C,Ramana CV,Kaipilyawar S,Krishnamurthy,Sivalenka S,Ambatipudi N,Gandhi S,Umashankar K,Cheyne J

    更新日期:2008-03-01 00:00:00

  • Development and decline of antiplasmodial indirect fluorescent antibodies in mice infected with Plasmodium berghei (NK65) and treated with drugs.

    abstract::Malaria parasites in mice present a simplified rodent model for the immunological study of malaria. Experiments have been performed to determine the pattern and persistence of malaria antibody as detected by the indirect fluorescent antibody (IFA) test utilizing specific antimouse IgM and IgG conjugates. The antibody ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Waki S,Suzuki M

    更新日期:1974-01-01 00:00:00

  • Validation of community health workers' assessment of neonatal illness in rural Bangladesh.

    abstract:OBJECTIVE:To estimate the validity (sensitivity, specificity, and positive and negative predictive values) of a clinical algorithm as used by community health workers (CHWs) to detect and classify neonatal illness during routine household visits in rural Bangladesh. METHODS:CHWs evaluated breastfeeding and symptoms an...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/blt.07.050666

    authors: Darmstadt GL,Baqui AH,Choi Y,Bari S,Rahman SM,Mannan I,Ahmed AS,Saha SK,Rahman R,Chang S,Winch PJ,Black RE,Santosham M,El Arifeen S,Bangladesh Projahnmo-2 (Mirzapur) Study Group.

    更新日期:2009-01-01 00:00:00

  • Screening of drugs and their assessment for use against the strobilate stage of Echinococcus.

    abstract::From time to time, reports of anthelmintic trials are published indicating that a compound is, or is not, effective against Echinococcus granulosus in dogs. In some of these reports, an attempt is made to assess the efficacy of the compound from inadequate data. This paper reviews the problems associated with selectin...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Gemmell MA

    更新日期:1968-01-01 00:00:00

  • Effect of removing user fees on attendance for curative and preventive primary health care services in rural South Africa.

    abstract::User fees are used to recover costs and discourage unnecessary attendance at primary care clinics in many developing countries. In South Africa, user fees for children aged under 6 years and pregnant women were removed in 1994, and in 1997 all user fees at all primary health care clinics were abolished. The intention ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Wilkinson D,Gouws E,Sach M,Karim SS

    更新日期:2001-01-01 00:00:00

  • ACUTE DIARRHOEAL DISEASE IN LESS DEVELOPED COUNTRIES. I. AN EIDEMIOLOGICAL BASIS FOR CONTROL.

    abstract::Some of the acute diarrhoeas of man are specific infectious diseases. Another and major proportion have no demonstrated infectious agent. No characteristic clinical pattern regularly distinguishes one specific disease entity from another, nor specific from non-specific infections, nor epidemic from endemic disease. Si...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: GORDON JE,BEHAR M,SCRIMSHAW NS

    更新日期:1964-01-01 00:00:00

  • International corporate tax avoidance and domestic government health expenditure.

    abstract:Objective:To compare estimated losses from international corporate tax avoidance in individual countries and domestic government health expenditure, with reference to the annual threshold of 86 United States dollars (US$) per capita required to achieve universal health coverage. Methods:I obtained and compared estimat...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.18.220897

    authors: O'Hare BA

    更新日期:2019-11-01 00:00:00

  • Mobilizing communities to improve maternal health: results of an intervention in rural Zambia.

    abstract:OBJECTIVE:To determine whether a complex community intervention in rural Zambia improved understanding of maternal health and increased use of maternal health-care services. METHODS:The intervention took place in six rural districts selected by the Zambian Ministry of Health. It involved community discussions on safe ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.13.122721

    authors: Ensor T,Green C,Quigley P,Badru AR,Kaluba D,Kureya T

    更新日期:2014-01-01 00:00:00

  • ELISA tests for dapsone and pyrimethamine and their application in a malaria chemoprophylaxis programme.

    abstract::Enzyme-linked immunosorbent asays (ELISAs) are described for determining levels of dapsone and pyrimethamine in urine. Both assays have a sensitivity of about 20 mug/l and are reproducible, but each produces some false positives. The problem of false positive reactions was partially obviated by requiring positive resu...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Greenwood BM,Greenwood AM,Bradley AK,Shenton FC,Smith AW,Snow RW,Williams K,Eggelte TA,Huikeshoven H,de Wit M

    更新日期:1986-01-01 00:00:00